The study identifies a variety of acquired antimicrobial resistance (AMR) genes in diarrhoeagenic Escherichia coli (DEC) strains from India, highlighting the prevalence of resistance to multiple antibiotic classes, including aminoglycosides, beta-lactams, sulfonamides, and tetracyclines. Key AMR genes include blaTEM-105, strB, strA, mphA, sul1, sul2, addA1-pm, tetA, tetB, blaCTX-M15, blaCTX-M27, and blaDHA-1. Additionally, mutations in the quinolone resistance-determining regions (QRDRs) of gyrA and parC were found to contribute to fluoroquinolone resistance.