Mutation Information

Protein Change

E50A, D75A, W117A, Q26A, Q116A, K201A, R212A, K194A, K215A

Organisms

Acinetobacter baumannii, Klebsiella pneumoniae

Confers Resistance To

polymyxins

Effect on Function

The E50A mutation disrupts SAM binding and reduces enzymatic activity, indicating a critical role in BioC function.; The D75A mutation disrupts SAM binding and reduces enzymatic activity, indicating a critical role in BioC function.; The W117A mutation disrupts SAM binding and reduces enzymatic activity, indicating a critical role in BioC function.; The Q26A mutation disrupts the neutralization of the malonyl group, affecting substrate binding and enzymatic activity.; The Q116A mutation disrupts the neutralization of the malonyl group, affecting substrate binding and enzymatic activity.; The K201A mutation affects the interaction with the ACP carrier, reducing enzymatic activity.; The R212A mutation affects the interaction with the ACP carrier, reducing enzymatic activity.; The K194A mutation affects the interaction with the ACP carrier, reducing enzymatic activity.; The K215A mutation affects the interaction with the ACP carrier, reducing enzymatic activity.

Validation Method

functional cloning and protein expression

Source Database

Reslit