The study identified an intrinsic resistome in Mycolicibacterium fortuitum comprising genes such as aph(3''-Ic), aac(2')-Ib, arr, blaF, erm39, rbpA, rox, and tap, which confer resistance to various antibiotics including macrolides, aminoglycosides, carbapenems, cephalosporins, and rifampicin. Additionally, a class 1 integron carrying qac/sul1 gene cassette was detected in one genome.