Browse AMR Genes
Explore antimicrobial resistance genes from the literature
Explore antimicrobial resistance genes from the literature
inhibitor-resistant carbapenem-hydrolyzing class A beta-lactamase KPC-100
Overview
| Protein Change | Nucleotide Change | Mechanism | Organism | Resistance To | Database | Validation Status |
|---|---|---|---|---|---|---|
| R164P | - | - | Enterobacter cloacae, E. coli | ceftazidime avibactamceftazidime|avibactam | Reslit | Candidate |
| D179H | - | - | Enterobacter cloacae, E. coli | ceftazidime avibactam | Reslit | Candidate |
| D179Y | - | - | Enterobacter cloacae, E. coli, Klebsiella pneumoniae | ceftazidime avibactamceftazidime|avibactam | Reslit | Candidate |
| T243M | - | - | Enterobacter cloacae, E. coli | ceftazidime avibactam | Reslit | Candidate |
| P104R | - | - | - | ceftazidime | Reslit | Candidate |
| L169M | - | - | - | ceftazidime|avibactam | Reslit | Candidate |
| A172D | - | - | - | ceftazidime|avibactam | Reslit | Candidate |
| V240G | - | - | Klebsiella pneumoniae | ceftazidime|avibactamceftazidime avibactam | Reslit | Candidate |
| Y241H | - | - | - | ceftazidime|avibactam | Reslit | Candidate |
| T243S | - | - | - | ceftazidime|avibactam | Reslit | Candidate |
| Y264H | - | - | - | ceftazidime|avibactam | Reslit | Candidate |
| H274N | - | - | - | ceftazidime|avibactam | Reslit | Candidate |
| K167R | - | decreased susceptibility to β-lactams, including the carbapenems, and meropenem-vaborbactam (MEM-VAB) | Klebsiella pneumoniae | imipenem relebactam|ceftazidime avibactam | Reslit | Candidate |
| - | - | Enterobacteriaceae, E. coli, Klebsiella pneumoniae | ceftazidime avibactam | Reslit | Candidate | |
| - | - | Enterobacteriaceae, E. coli, Klebsiella pneumoniae | ceftazidime avibactam | Reslit | Candidate | |
| - | - | Enterobacteriaceae, E. coli, Klebsiella pneumoniae | ceftazidime avibactam | Reslit | Candidate | |
| - | - | Enterobacteriaceae, E. coli, Klebsiella pneumoniae | ceftazidime avibactam | Reslit | Candidate |
| Allele | Database | Papers | Drug Classes | Organisms | Countries | Years | Sequence Accession | Protein Accession |
|---|---|---|---|---|---|---|---|---|
| blaKPC-100 | Card DatabaseReference Gene CatalogReslit | 3 | CARBAPENEM, ceftazidime avibactam | Klebsiella pneumoniae | China | 2024 | ON521726.1 | URC16703.1 |
| blaKPC-101 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OK086805.1 | UBJ91322.1 |
| blaKPC-102 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OK652013.1 | UDU33959.1 |
| blaKPC-103 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae subsp. pneumoniae | - | - | OL445423.1 | UFK32670.1 |
| blaKPC-105 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae subsp. pneumoniae | - | - | OL445426.1 | UFK32673.1 |
| blaKPC-109 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae subsp. pneumoniae | - | - | OL744263.1 | UGY69815.1 |
| blaKPC-110 | Card DatabaseReference Gene CatalogReslit | 3 | CEPHALOSPORIN, ceftazidime avibactam | Klebsiella pneumoniae +1 | Italy | 2023 | CP100313.1 | UIH96895.1 |
| blaKPC-111 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae subsp. pneumoniae | - | - | OL744330.1 | UIX50813.1 |
| blaKPC-115 | Card DatabaseReference Gene CatalogReslit | 3 | CEPHALOSPORIN, ceftazidime avibactam | Klebsiella pneumoniae subsp. pneumoniae +1 | Argentina | 2022 | OM714909.1 | ULU82668.1 |
| blaKPC-116 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OM729575.1 | ULU82669.1 |
| blaKPC-117 | Card DatabaseReference Gene CatalogReslit | 3 | CARBAPENEM, carbapenems | Klebsiella pneumoniae +1 | - | 2025 | OM933711.1 | UNN26047.1 |
| blaKPC-118 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OM933712.1 | UNN26048.1 |
| blaKPC-119 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OM933713.1 | UNN26049.1 |
| blaKPC-120 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Pseudomonas aeruginosa | - | - | OM933715.1 | UNN26051.1 |
| blaKPC-122 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Enterobacter cloacae | - | - | OM933720.1 | UNN26056.1 |
| blaKPC-124 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | ON221403.1 | UOX08352.1 |
| blaKPC-126 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OM830488.1 | UPY21302.1 |
| blaKPC-127 | Card DatabaseReference Gene CatalogReslit | 3 | CARBAPENEM, ceftazidime avibactam | Klebsiella pneumoniae | China | 2024 | ON521725.1 | URC16702.1 |
| blaKPC-128 | Card DatabaseReference Gene CatalogReslit | 3 | CARBAPENEM, ceftazidime avibactam | Klebsiella pneumoniae | China | 2024 | ON521727.1 | URC16704.1 |
| blaKPC-129 | Card DatabaseReference Gene CatalogReslit | 3 | CARBAPENEM, ceftazidime avibactam | Klebsiella pneumoniae | China | 2024 | ON751738.1 | USG13864.1 |
| blaKPC-130 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | ON794466.1 | USK12023.1 |
| blaKPC-131 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae subsp. pneumoniae | - | - | ON823194.1 | USQ89943.1 |
| blaKPC-132 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OP081092.1 | UUF81222.1 |
| blaKPC-135 | Card DatabaseReference Gene CatalogReslit | 4 | CARBAPENEM, ceftazidime avibactam | Klebsiella pneumoniae | China | 2024 | OP205646.1 | UVV34299.1 |
| blaKPC-136 | Card DatabaseReference Gene CatalogReslit | 3 | CARBAPENEM, ceftazidime avibactam | Klebsiella pneumoniae | China | 2024 | OQ579152.1 | WEG44273.1 |
| blaKPC-137 | Card DatabaseReference Gene CatalogReslit | 3 | CARBAPENEM, ceftazidime avibactam | Klebsiella pneumoniae | China | 2024 | OR596987.1 | WNR58488.1 |
| blaKPC-138 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | OP432320.1 | UXO98494.1 |
| blaKPC-139 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OP503887.1 | UXO99692.1 |
| blaKPC-140 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OP503888.1 | UXO99693.1 |
| blaKPC-141 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OP503889.1 | UXO99694.1 |
| blaKPC-142 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OP503890.1 | UXO99695.1 |
| blaKPC-143 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OP503891.1 | UXO99696.1 |
| blaKPC-144 | Card DatabaseReference Gene CatalogReslit | 4 | CARBAPENEM, ceftazidime avibactam | Klebsiella pneumoniae | China, Europe | 2024, 2025 | OP559533.1 | UXP87111.1 |
| blaKPC-146 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Citrobacter freundii | - | - | OP696903.1 | UZC76861.1 |
| blaKPC-147 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OP696904.1 | UZC76862.1 |
| blaKPC-148 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | JAOZYA010000028.1 | MCW0253507.1 |
| blaKPC-149 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OP778041.1 | UZF98429.1 |
| blaKPC-150 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OP787490.1 | UZH56920.1 |
| blaKPC-151 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | OP823148.1 | UZQ18806.1 |
| blaKPC-152 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae subsp. pneumoniae | - | - | OP856888.1 | UZT70951.1 |
| blaKPC-153 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | OP884096.1 | UZZ47414.1 |
| blaKPC-155 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | OQ139542.1 | WBG96004.1 |
| blaKPC-156 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OQ390084.1 | WCS94746.1 |
| blaKPC-157 | Card DatabaseReference Gene CatalogReslit | 3 | CARBAPENEM, imipenem relebactam | Klebsiella huaxiensis +2 | Europe | 2025 | JAPQEX020000004.1 | MDG1646356.1 |
| blaKPC-159 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | OQ450354.1 | WDQ79090.1 |
| blaKPC-160 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OQ579136.1 | WEG44274.1 |
| blaKPC-161 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OQ579137.1 | WEG44275.1 |
| blaKPC-162 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OQ579138.1 | WEG44276.1 |
| blaKPC-164 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OQ579140.1 | WEG44278.1 |
| blaKPC-165 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OQ579141.1 | WEG44279.1 |
| blaKPC-166 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OQ592369.1 | WEM34776.1 |
| blaKPC-178 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OQ926587.1 | WGW18280.1 |
| blaKPC-180 | Card DatabaseReference Gene CatalogReslit | 3 | CARBAPENEM, ceftazidime avibactam | Klebsiella pneumoniae | China | 2024 | OR206047.1 | WJR95055.1 |
| blaKPC-181 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OR282795.1 | WKT28610.1 |
| blaKPC-182 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OR282796.1 | WKT28611.1 |
| blaKPC-183 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OR282800.1 | WKT28615.1 |
| blaKPC-184 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OR282801.1 | WKT28616.1 |
| blaKPC-185 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | OR359279.1 | WLF01972.1 |
| blaKPC-186 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | OR466746.1 | WMI40670.1 |
| blaKPC-187 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OR466751.1 | WMI45073.1 |
| blaKPC-188 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OR483145.1 | WMP15088.1 |
| blaKPC-190 | Card DatabaseReference Gene CatalogReslit | 3 | CARBAPENEM, ceftazidime avibactam +1 | Klebsiella pneumoniae +1 | China | 2025 | OR499110.1 | WMQ75103.1 |
| blaKPC-191 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OR499111.1 | WMQ75104.1 |
| blaKPC-192 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OR529436.1 | WMZ17075.1 |
| blaKPC-193 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OR568565.1 | WNH41912.1 |
| blaKPC-194 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | OR578928.1 | WNI39959.1 |
| blaKPC-195 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OR590804.1 | WNR58177.1 |
| blaKPC-196 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OR612035.1 | WNV60940.1 |
| blaKPC-199 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae subsp. pneumoniae | - | - | OR672047.1 | WOF01111.1 |
| blaKPC-20 | Reference Gene Catalog | 1 | CARBAPENEM | Citrobacter youngae | - | - | JBPQFE010000002.1 | MGK4321900.1 |
| blaKPC-200 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae subsp. pneumoniae | - | - | OR672048.1 | WOF01112.1 |
| blaKPC-201 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Pseudomonas aeruginosa | - | - | ON297687.1 | USV27793.1 |
| blaKPC-202 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OR839185.1 | WPM47153.1 |
| blaKPC-204 | Card DatabaseReference Gene CatalogReslit | 3 | CARBAPENEM, carbapenems +1 | Klebsiella pneumoniae +1 | - | 2025 | OR979533.1 | WQQ44875.1 |
| blaKPC-206 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Enterobacter cloacae | - | - | PP051499.1 | WQY95155.1 |
| blaKPC-207 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | PP125175.1 | WRW33974.1 |
| blaKPC-208 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | PP125176.1 | WRW33975.1 |
| blaKPC-209 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Pseudomonas aeruginosa | - | - | PP165529.1 | WVD92252.1 |
| blaKPC-210 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | PP140442.1 | WVD92251.1 |
| blaKPC-211 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | PP194258.1 | WVD92244.1 |
| blaKPC-212 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | PP194259.1 | WVD92245.1 |
| blaKPC-213 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | PP194260.1 | WVD92246.1 |
| blaKPC-214 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | PP194261.1 | WVD92247.1 |
| blaKPC-215 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | PP194263.1 | WVD92249.1 |
| blaKPC-217 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Escherichia coli | - | - | PP693362.1 | WZW61255.1 |
| blaKPC-218 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | PP693363.1 | WZW61256.1 |
| blaKPC-219 | Reference Gene Catalog | 1 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | PP683429.1 | WZW61415.1 |
| blaKPC-22 | Card DatabaseReference Gene CatalogResFinder Database | 4 | AZTREONAM, MEROPENEM +15 | Klebsiella pneumoniae | - | - | KM379100.1 | AIX87991.1 |
| blaKPC-220 | Reference Gene Catalog | 1 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | PP683430.1 | WZW61416.1 |
| blaKPC-221 | Reference Gene Catalog | 1 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | PP683431.1 | WZW61417.1 |
| blaKPC-222 | Reference Gene Catalog | 1 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | PP683432.1 | WZW61418.1 |
| blaKPC-223 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | PP701811.1 | WZW71004.1 |
| blaKPC-225 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Escherichia coli | - | - | PP723735.1 | XAM74258.1 |
| blaKPC-226 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | PP770481.1 | XAR02267.1 |
| blaKPC-227 | Card DatabaseReference Gene CatalogReslit | 3 | CARBAPENEM, carbapenems +1 | Klebsiella pneumoniae +1 | - | 2025 | PP770482.1 | XAR02237.1 |
| blaKPC-228 | Card DatabaseReference Gene CatalogResFinder Database | 4 | AZTREONAM, MEROPENEM +15 | Klebsiella pneumoniae | - | 2025 | PP860922.1 | XBP46894.1 |
| blaKPC-229 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | PP870933.1 | XBP46906.1 |
| blaKPC-230 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | PP988013.1 | XCN99469.1 |
| blaKPC-231 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | PQ117760.1 | XFH17878.1 |
| blaKPC-232 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | PQ117762.1 | XFH17880.1 |
| blaKPC-233 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | PQ287843.1 | XGD00488.1 |
| blaKPC-234 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | PQ311675.1 | XHE66866.1 |
| blaKPC-236 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | PQ394549.1 | XHO32892.1 |
| blaKPC-237 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | PQ394550.1 | XHO32893.1 |
| blaKPC-238 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | PQ394551.1 | XHO32894.1 |
| blaKPC-239 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | PQ394552.1 | XHO32895.1 |
| blaKPC-240 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | PQ394553.1 | XHO32896.1 |
| blaKPC-241 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | PQ394554.1 | XHO32897.1 |
| blaKPC-242 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | PQ394555.1 | XHO32898.1 |
| blaKPC-243 | Reference Gene Catalog | 1 | CARBAPENEM | Pseudomonas aeruginosa | - | - | PQ571658.1 | XKQ46068.1 |
| blaKPC-244 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | ABEZTZ040000033.1 | MFH7848025.1 |
| blaKPC-245 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | JBJGWA010000036.1 | MFL3882384.1 |
| blaKPC-246 | Reference Gene Catalog | 1 | CARBAPENEM | Pseudomonas aeruginosa | - | - | PQ666868.1 | XMD53573.1 |
| blaKPC-247 | Reference Gene Catalog | 1 | CARBAPENEM | Pseudomonas aeruginosa | - | - | PQ666870.1 | XMD53575.1 |
| blaKPC-248 | Reference Gene Catalog | 1 | CEPHALOSPORIN | Klebsiella michiganensis | - | - | PQ666871.1 | XMD53576.1 |
| blaKPC-249 | Reference Gene Catalog | 1 | CARBAPENEM | Pseudomonas aeruginosa | - | - | PQ763404.1 | XLV50109.1 |
| blaKPC-250 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | JBJJSZ010000066.1 | MFL1755427.1 |
| blaKPC-251 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | PQ825746.1 | XLZ96863.1 |
| blaKPC-252 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | PQ881806.1 | XNX56389.1 |
| blaKPC-255 | Reference Gene Catalog | 1 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | PV068515.1 | XOD94989.1 |
| blaKPC-256 | Reference Gene Catalog | 1 | CARBAPENEM | Escherichia coli | - | - | PV075146.1 | XOU30610.1 |
| blaKPC-257 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | PV075145.1 | XOU30609.1 |
| blaKPC-258 | Reference Gene Catalog | 1 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | PV103143.1 | XOU30689.1 |
| blaKPC-259 | Reference Gene Catalog | 1 | CEPHALOSPORIN | Pseudomonas aeruginosa | - | - | PV103221.1 | XPD06450.1 |
| blaKPC-260 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | PV105721.1 | XPA07738.1 |
| blaKPC-261 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | PV107385.1 | XPA07742.1 |
| blaKPC-262 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | JBLMKQ010000073.1 | MFU1763767.1 |
| blaKPC-263 | Reference Gene CatalogReslit | 2 | CARBAPENEM, ceftazidime | Klebsiella pneumoniae +1 | China | 2025 | PV219192.1 | XPO98277.1 |
| blaKPC-265 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | PV290226.1 | XQH02550.1 |
| blaKPC-266 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | PV335540.1 | XQJ32191.1 |
| blaKPC-267 | Reference Gene Catalog | 1 | CEPHALOSPORIN | Pseudomonas aeruginosa | - | - | PV357208.1 | XQU44397.1 |
| blaKPC-268 | Reference Gene Catalog | 1 | CEPHALOSPORIN | Pseudomonas aeruginosa | - | - | PV357209.1 | XQU44398.1 |
| blaKPC-269 | Reference Gene Catalog | 1 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | PV454371.1 | XRI57705.1 |
| blaKPC-270 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | PV487508.1 | XRI57713.1 |
| blaKPC-271 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | PV530466.1 | XRP75571.1 |
| blaKPC-272 | Reference Gene Catalog | 1 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | PV615266.1 | XTK91176.1 |
| blaKPC-273 | Reference Gene Catalog | 1 | CARBAPENEM | Pseudomonas aeruginosa | - | - | PV626809.1 | XTP75245.1 |
| blaKPC-274 | Reference Gene Catalog | 1 | CARBAPENEM | Pseudomonas aeruginosa | - | - | PV626810.1 | XTP75246.1 |
| blaKPC-275 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | PV626811.1 | XTP75247.1 |
| blaKPC-277 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | PV666438.1 | XTZ51667.1 |
| blaKPC-278 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | PV666439.1 | XTZ51668.1 |
| blaKPC-279 | Reference Gene Catalog | 1 | CARBAPENEM | Pseudomonas aeruginosa | - | - | PV759336.1 | XVC13674.1 |
| blaKPC-280 | Reference Gene Catalog | 1 | CARBAPENEM | Pseudomonas aeruginosa | - | - | PV764590.1 | XVF92898.1 |
| blaKPC-281 | Reference Gene Catalog | 1 | CARBAPENEM | Escherichia coli | - | - | PV791199.1 | XWJ61549.1 |
| blaKPC-282 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae subsp. pneumoniae | - | - | PX119674.1 | XZC91449.1 |
| blaKPC-283 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | PV809687.1 | XXM93802.1 |
| blaKPC-284 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | PV809688.1 | XXM93803.1 |
| blaKPC-285 | Reference Gene Catalog | 1 | CARBAPENEM | Pseudomonas aeruginosa | - | - | PX640693.1 | YBZ79746.1 |
| blaKPC-286 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | PX710159.1 | YCB57839.1 |
| blaKPC-287 | Reference Gene Catalog | 1 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | PX776553.1 | YCJ22072.1 |
| blaKPC-288 | Reference Gene Catalog | 1 | CARBAPENEM | Pseudomonas aeruginosa | - | - | PX845592.1 | YCN21071.1 |
| blaKPC-289 | Reference Gene Catalog | 1 | CEPHALOSPORIN | Pseudomonas aeruginosa | - | - | PX854775.1 | YCO05609.1 |
| blaKPC-290 | Reference Gene Catalog | 1 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | PX880058.1 | YCQ72179.1 |
| blaKPC-291 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | JBUCKA010000075.1 | MHC9504171.1 |
| blaKPC-292 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | PX970391.1 | YCZ31967.1 |
| blaKPC-293 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | JBUPVD010000036.1 | MHF0017214.1 |
| blaKPC-294 | Reference Gene Catalog | 1 | CARBAPENEM | Enterobacter cloacae | - | - | PZ017069.1 | YDC19163.1 |
| blaKPC-295 | Reference Gene Catalog | 1 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | JBUAPU010000030.1 | MHC6674589.1 |
| blaKPC-296 | Reference Gene Catalog | 1 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | JBUPVG010000037.1 | MHF0091084.1 |
| blaKPC-297 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | - | PX910544.1 | YDO78657.1 |
| blaKPC-298 | Reference Gene Catalog | 1 | CARBAPENEM | Pseudomonas aeruginosa | - | - | PZ259408.1 | YDV00491.1 |
| blaKPC-299 | Reference Gene Catalog | 1 | CARBAPENEM | Pseudomonas aeruginosa | - | - | PZ335140.1 | YEM37956.1 |
| blaKPC-37 | Card DatabaseReference Gene CatalogResFinder Database | 4 | AZTREONAM, MEROPENEM +15 | Klebsiella pneumoniae | - | - | MH718730.1 | AXL13972.1 |
| blaKPC-38 | Card DatabaseReference Gene CatalogResFinder Database | 4 | AZTREONAM, MEROPENEM +15 | Klebsiella pneumoniae | - | - | MK098861.1 | AYP70144.1 |
| blaKPC-42 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | MK467612.1 | QBC75465.1 |
| blaKPC-43 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | MK628511.1 | QBO66649.1 |
| blaKPC-45 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Enterobacter cloacae | - | - | MN104596.1 | QDM39441.1 |
| blaKPC-54 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | MN854706.1 | QHA33651.1 |
| blaKPC-56 | Card DatabaseReference Gene CatalogReslit | 3 | CARBAPENEM, carbapenems | Klebsiella pneumoniae | United States | 2022 | MT040751.1 | QIC04092.1 |
| blaKPC-58 | Card DatabaseReference Gene CatalogReslit | 4 | CARBAPENEM, carbapenems | Klebsiella pneumoniae | United States | 2022 | MT463289.1 | QJX44695.1 |
| blaKPC-59 | Card DatabaseReference Gene CatalogReslit | 4 | CARBAPENEM, carbapenems | Klebsiella pneumoniae | United States | 2022 | MT463290.1 | QJX44696.1 |
| blaKPC-60 | Card DatabaseReference Gene Catalog | 3 | CARBAPENEM | Escherichia coli | - | - | MT482411.1 | QJX44698.1 |
| blaKPC-73 | Card DatabaseReference Gene Catalog | 3 | CEPHALOSPORIN | Klebsiella pneumoniae | - | - | MT833886.1 | QNC69633.1 |
| blaKPC-75 | Card DatabaseReference Gene Catalog | 3 | CARBAPENEM | Klebsiella pneumoniae | - | - | MT920645.1 | QNL15886.1 |
| blaKPC-77 | Card DatabaseReference Gene Catalog | 3 | CARBAPENEM | Klebsiella pneumoniae | - | - | MW030519.1 | QOJ39190.1 |
| blaKPC-80 | Card DatabaseReference Gene Catalog | 3 | CARBAPENEM | Klebsiella pneumoniae | - | - | MW444845.1 | QQL91944.1 |
| blaKPC-81 | Card DatabaseReference Gene Catalog | 3 | CARBAPENEM | Klebsiella pneumoniae | - | - | MW444846.1 | QQL91945.1 |
| blaKPC-83 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Aeromonas caviae | - | - | MW581775.1 | QRM14288.1 |
| blaKPC-85 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | MW896839.1 | QTV25836.1 |
| blaKPC-89 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | MZ401141.1 | QWW21252.1 |
| blaKPC-91 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | MZ404505.1 | QWW21254.1 |
| blaKPC-97 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | - | OK086971.1 | UAY85937.1 |
| blaKPC-99 | Card DatabaseReference Gene CatalogReslit | 3 | CARBAPENEM, carbapenems | Klebsiella pneumoniae +1 | China | 2025 | OK086803.1 | UBJ91320.1 |
| blaKPC-125 | Card DatabaseReference Gene Catalog | 3 | CARBAPENEM | Klebsiella pneumoniae | - | 2022 | NG_080778.1 | WP_248496376.1 |
| blaKPC-21 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 6 | carbapenems, AZTREONAM +17 | Escherichia coli +1 | Portugal | 2018 | NG_049254.1 | WP_063860711.1 |
| blaKPC-23 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 5 | carbapenems, cephalosporins +17 | Klebsiella pneumoniae +1 | Greece | 2019 | MH450213.1 | AWU66461.1 |
| blaKPC-25 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 7 | AZTREONAM, MEROPENEM +18 | Klebsiella pneumoniae +1 | China | 2017, 2024, 2025 | NG_051167.1 | WP_065419571.1 |
| blaKPC-44 | Card DatabaseReference Gene CatalogReslit | 4 | CARBAPENEM, ceftazidime +1 | Klebsiella pneumoniae +1 | - | 2019, 2024 | NG_065427.1 | WP_140423311.1 |
| blaKPC-61 | Card DatabaseReference Gene Catalog | 3 | CEPHALOSPORIN | Klebsiella pneumoniae | - | 2021 | NG_070180.1 | WP_179284328.1 |
| blaKPC-62 | Card DatabaseReference Gene CatalogReslit | 4 | ceftazidime avibactam, CEFIDEROCOL +1 | Klebsiella pneumoniae | Italy|Puerto Rico|USA|Greece|France|China|Switzerland|Spain | 2021 | NG_073465.1 | WP_204376229.1 |
| blaKPC-63 | Card DatabaseReference Gene CatalogReslit | 4 | ceftazidime avibactam, CEPHALOSPORIN | Klebsiella pneumoniae | Italy|Puerto Rico|USA|Greece|France|China|Switzerland|Spain | 2021 | NG_073466.1 | WP_204376230.1 |
| blaKPC-64 | Card DatabaseReference Gene CatalogReslit | 4 | ceftazidime avibactam, CEPHALOSPORIN | Klebsiella pneumoniae | Italy|Puerto Rico|USA|Greece|France|China|Switzerland|Spain | 2021 | NG_073467.1 | WP_204376231.1 |
| blaKPC-65 | Card DatabaseReference Gene CatalogReslit | 4 | ceftazidime avibactam, CEPHALOSPORIN | Klebsiella pneumoniae | Italy|Puerto Rico|USA|Greece|France|China|Switzerland|Spain | 2021 | NG_073468.1 | WP_204376232.1 |
| blaKPC-66 | Card DatabaseReference Gene CatalogReslit | 5 | ceftazidime avibactam, CEPHALOSPORIN | Klebsiella pneumoniae | Italy, Spain | 2021, 2022 | NG_070739.1 | WP_188331871.1 |
| blaKPC-71 | Card DatabaseReference Gene CatalogReslit | 8 | ceftazidime avibactam, CEPHALOSPORIN | Klebsiella pneumoniae +5 | China, Europe, China|Jiangxi, China | 2021, 2022, 2025 | NG_070895.1 | WP_194293134.1 |
| blaKPC-72 | Card DatabaseReference Gene Catalog | 3 | CEPHALOSPORIN | Klebsiella pneumoniae | - | 2024 | NG_070740.1 | WP_188331872.1 |
| blaKPC-74 | Card DatabaseReference Gene CatalogReslit | 4 | ceftazidime avibactam, CEPHALOSPORIN | Klebsiella pneumoniae +1 | China | 2021 | NG_070742.1 | WP_188331874.1 |
| blaKPC-76 | Card DatabaseReference Gene CatalogReslit | 4 | ceftazidime avibactam, CEPHALOSPORIN | Klebsiella pneumoniae | China | 2021 | NG_070896.1 | WP_194293135.1 |
| blaKPC-78 | Card DatabaseReference Gene CatalogReslit | 4 | ceftazidime avibactam, CARBAPENEM | Klebsiella pneumoniae | China | 2021 | NG_071204.1 | WP_197749403.1 |
| blaKPC-79 | Card DatabaseReference Gene CatalogReslit | 4 | ceftazidime avibactam, CEPHALOSPORIN | Klebsiella pneumoniae | China | 2021 | NG_071205.1 | WP_197749404.1 |
| blaKPC-82 | Card DatabaseReference Gene CatalogReslit | 4 | ceftazidime avibactam, CEPHALOSPORIN | Citrobacter koseri +1 | - | 2021 | NG_073471.1 | WP_202781289.1 |
| blaKPC-9 | Card DatabaseResFinder DatabaseReslit | 4 | carbapenems, ceftazidime +16 | Escherichia coli +1 | Israel, Italy | 2012, 2021 | FJ624872.1 | ACM91559.1 |
| blaKPC-90 | Card DatabaseReference Gene CatalogReslit | 6 | ceftazidime avibactam, CEPHALOSPORIN +2 | Pseudomonas aeruginosa +3 | China | 2022, 2025 | MZ570431.1 | QXT58056.1 |
| blaKPC-1 | Reslit | 10 | imipenem, meropenem +5 | Klebsiella pneumoniae +5 | New York City, Mexico, United States, Brazil, Japan|France|United Kingdom|North America|South America|Europe|Asia/Oceania|Africa, Denmark|China|Japan|United States|Spain|Portugal|Thailand|UK, China, United Arab Emirates | 2001, 2006, 2015, 2019, 2020, 2022, 2023, 2025 | AF297554 | - |
| blaKPC-2 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 621 | imipenem, ertapenem +42 | Klebsiella pneumoniae +97 | United States, Maryland, New York City, Israel, United States|Colombia|Greece, China, France|Israel, Eastern Europe|India|Latin America|Asia Pacific|North America|Western Europe, Eastern USA, Europe|USA, New Jersey|Ohio, Northeast Ohio, USA|Greece|Sweden|Colombia|Brazil|Israel, New York|New Jersey, Europe, Greece|Spain|Taiwan|Turkey|Colombia|Puerto Rico|USA|Argentina|Chile|Germany|Italy|Philippines|Thailand, Korea, South Africa, Brazil, Florida|Colorado|Oklahoma|New Jersey, Greece, Europe|Asia|North America|South America, Taiwan, Norway, USA|Colombia|Israel|Brazil, Zhejiang Province, Switzerland, India|Greece|Israel|Ecuador, New Jersey|New York, Americas, Western Pennsylvania, Germany, Uruguay, Cuba, Venezuela, Ontario, Canada, Netherlands, Italy|South America, northeastern Ohio, United States|Boston, MA, India, Middle East|Afghanistan|Iraq|Egypt|Pakistan|Lebanon|Iran|Jordan|Persian Gulf Countries|United Arab Emirates|Kuwait|Qatar|Bahrain|Oman|Saudi Arabia|Turkey, United States|Spain|Italy|China, UK, USA|Global, Mainland China, Northern China, Puerto Rico, United States|Europe|Asia|Africa|South America|India|United Kingdom|Egypt|Belgium|Italy|China|United Arab Emirates, United States|New York|Florida|Illinois|Gaza|Colombia|South America|Mediterranean region, United States|Europe|Asia-Pacific|Latin America, Houston, Texas|Texas, Eastern China, Vietnam, Europe|Spain, South Korea, Oltrepò Pavese Area, Northern Italy|Italy, Singapore, China|France|Brazil|Italy, Victoria, Australia, Jordan, Japan|Taiwan, Hong Kong, Shanghai, China, United States|Colombia|Australia|India|China|France|Germany|Egypt|Switzerland|Pakistan|Morocco|Tunisia|Taiwan, Mexico, Northwest China, South Carolina, Gothenburg, Spain, France, St. Louis, MO, USA, Japan, Colombia, Italy, Finland, Tokyo Bay|Japan, US, Puerto Rico|Dominican Republic, New York State, Thailand, Saudi Arabia, United States|China|Hong Kong, Recife-PE, Brazil, Italy|Colombia|United States|South Africa|India|Greece|North Africa, Portugal, global, China|Taiwan, Africa|Asia|South Asia, Europe|Poland, Northeast China, Eastern Europe|Western Europe, Czech Republic, Southwest China, Brasília, Brazil, Argentina, Cleveland Clinic, OH, Hanoi, Vietnam|Vietnam, Japan|New York|Montreal, Europe|United States|Latin America|Asia-Pacific, China|Henan province, China, Barcelona, Spain, Anhui province, Egypt, Beijing, China, Milwaukee, Wisconsin, USA|USA, Kuwait, Boston, MA|Orange, CA, Southwestern China, central Adriatic Sea|Croatia|Croatian marine environment, Northeastern Ohio, Zhejiang Province, China, United States|France|Italy|Switzerland|Greece|China|Germany|Spain|Japan|UK|Canada|Australia, East Asia|Middle East, South America, Houston, Texas|United States, Croatia, Romania|Canada, southeastern China, Zhejiang, China|China, Bulgaria, Montevideo, Brazilian Amazon, various countries|Global, Zagreb, Croatia, Rio de Janeiro, Guangdong, China, Europe|Asia|North America|Western and South-Eastern Asia, China|Colombia|Lebanon|Singapore|United States, Chile, Maryland|California|New York|Pennsylvania, China|Thailand|United States|United Kingdom|Europe|Northern Africa|Middle East, Lower Saxony, Germany, New Orleans metropolitan area, Mexico|Mexico City, Global, South China, Qinghai Lake, China, South Africa|Spain|Netherlands|Switzerland|India|China|Germany|Russia|USA|Australia|Other countries, Shanxi Province|Shanxi Province, China, Wuhan, China, Saint-Petersburg, USA|China|India|Thailand|Brazil|Hungary|South Africa|Egypt|Tunisia|Europe|Asia, Austria, Beijing, China|Poland, Beijing|China, Saudi Arabia|hospital, Peru|Spain, Bangladesh|Brazil|Greece|Italy|Kenya|South Africa|Thailand|Uganda|Vietnam, Northern Italy, Portugal|Spain, Brazil|Egypt, Asia/South Pacific|Europe|Latin America|Middle East/Africa|North America, Shenzhen, China, Ecuador, Gaza Strip, USA|China|Japan|Spain|United Kingdom|Germany|France|South Korea|Netherlands|Viet Nam|Laos|Canada|Czech Republic|Argentina|Brazil|Belgium|Denmark|Iran|Australia|Croatia|Ghana|Mexico|Missing, northern China, Peru, Europe|North America|Asia|Southern Europe|Western Asia, Anhui Province, China|Singapore|Vietnam|USA|Ireland|Russia|Bangladesh|Thailand|Germany, Europe|Italy, Ningbo, Europe|Switzerland, Europe|China|Thailand, Philippines, China|Southwest China, Northeast India|India, Southern China, Europe|United States, Argentina|Canada|Germany|United States, China|Europe|Americas|Asia|North Africa|Middle East, China|Egypt, India|United States, Quebec|Shenzhen Bay, China|Europe|India|United Arab Emirates|Greece|Switzerland, Asia|Europe, New Zealand|United Kingdom|United States|Tunisia|Czechia, Henan, China, China|Australia, Europe|Austria, Europe|Asia|North America, Central China, Brazil|Thailand, Dhaka|Cox's Bazar|Bangladesh | 2002, 2003, 2005, 2006, 2007, 2008, 2009, 2010, 2011, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021, 2022, 2023, 2024, 2025, 2026 | AF055067|X77455|X91840|U58495|X04730 | AAK70220.1 |
| blaKPC-3 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 177 | carbapenems, AZTREONAM +34 | Klebsiella pneumoniae +45 | New York, New York City, Eastern USA, New Jersey|Ohio, Israel, Northeast Ohio, New York|New Jersey, China, Greece|Spain|Taiwan|Turkey|Colombia|Puerto Rico|USA|Argentina|Chile|Germany|Italy|Philippines|Thailand, United States, New Jersey|New York, India|Greece|Israel|Ecuador, Italy, Western Pennsylvania, Germany, North Dakota, USA, Puerto Rico, Ontario, Canada, United States|Boston, MA, Poland, Minnesota|North Dakota, UK, United States|Europe|Asia|Africa|South America|India|United Kingdom|Egypt|Belgium|Italy|China|United Arab Emirates, North Carolina, United States|New York|Florida|Illinois|Gaza|Colombia|South America|Mediterranean region, United States|India|China|Belgium|Brazil|Canada|Colombia|Greece|Turkey, Afghanistan|Italy|Germany|United States, Oltrepò Pavese Area, Northern Italy|Italy, South Korea, Sweden, Portugal, Eastern China, Greece, Spain, Europe|Switzerland, Latium region, US, Colombia, New York State, United States|China|Hong Kong, Italy|Colombia|United States|South Africa|India|Greece|North Africa, global, Dominican Republic, Porto, Portugal, Peru, Eastern Europe|Western Europe, Europe, Europe|United States|Latin America|Asia-Pacific, Europe|United States|Taiwan, Barcelona, Spain, Boston, MA|Orange, CA, Europe|Portugal, Korea, Washington State, United States|France|Italy|Switzerland|Greece|China|Germany|Spain|Japan|UK|Canada|Australia, Spain|Portugal, Switzerland, Beijing, China, various countries|Global, Europe|Asia|North America|Western and South-Eastern Asia, Maryland|California|New York|Pennsylvania, Boston|California|New York, Russia|Switzerland|Italy|USA|Norway|Spain|Ghana, Mexico|Mexico City, Sardinia, Italy, China|Germany|USA, Global, India, China|Poland, Northern Italy, Ningbo|China, Shenzhen, China, Ecuador, Mexico, Europe|Czech Republic, Brazil, France, Europe|Italy, Europe|United States, Argentina, Canada, India|United States, Europe|Austria, Europe|Asia|North America | 2004, 2006, 2009, 2010, 2011, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021, 2022, 2023, 2024, 2025 | AF395881|AF482468 | AAL05630.1 |
| blaKPC | Reslit | 471 | carbapenems, imipenem +25 | Klebsiella pneumoniae +130 | United States, Argentina, Israel, New York City, Canada, USA, Trinidad and Tobago, South Florida, Italy, France, Michigan, China, Europe, Washington|Alaska|New Jersey|Malaysia|Mexico|Israel, Singapore, Brazil, São Paulo|Brazil, Worldwide, United States|Spain, India, Iran, New Jersey, Japan, Germany, Los Angeles, California, Brazil|United States, Quebec, Canada, United States|Italy|Israel|Greece|New York|New Jersey, Belgium, Korea, Middle East|Afghanistan|Iraq|Egypt|Pakistan|Lebanon|Iran|Jordan|Persian Gulf Countries|United Arab Emirates|Kuwait|Qatar|Bahrain|Oman|Saudi Arabia|Turkey, USA|Global, Argentina|Colombia|Ecuador|Guatemala|Mexico|Peru|Venezuela, United States|United Kingdom|Spain, United States|Italy|Israel|India|Turkey, Medellín, Colombia, Sweden, United States|Europe|Asia|Africa|South America|India|United Kingdom|Egypt|Belgium|Italy|China|United Arab Emirates, United States|New York|Florida|Illinois|Gaza|Colombia|South America|Mediterranean region, Spain, Saudi Arabia, global, Bangladesh, Norway, Victoria, Australia, Turkey, United Kingdom, New York City|Boston|United Kingdom|Minnesota|North Dakota|New Jersey|Pennsylvania|Michigan|United States, India|United States, Heilongjiang Province, China, Egypt, Japan|Myanmar|Singapore|Thailand, Toronto|Canada, Mexico City, Northern California, Italy|Germany, UK, La Paz, Bolivia|La Paz River basin, Lebanon, Thailand, Taiwan, Global, Ireland, Shenzhen, southern China, South Korea, Henan, China, Philippines, United States|India|Turkey|Europe, Poland, Shanghai, China, North Carolina|Florida, Brazilian Amazon|Brazil, Khartoum, Sudan, Nepal, Brazil|Czech Republic|Germany|India|Japan|Kenya|Kuwait|Nigeria|Philippines|South Korea|Thailand|United States, Colombia, South Africa, Eastern Cape Province, Republic of South Africa|South Africa, Paraíba, Brazil, Dominican Republic, Taiwan|Taipei, Taiwan, Pakistan, Guangzhou, China, Europe|Israel, Nigeria, Chile, UK|USA|France|Spain|Italy|Europe, Germany|Arabian Peninsula, Europe|Iraq, Asia|Pacific, Asia|Hong Kong, New Zealand, Brasília, Brazil, Europe|Greece, Mexico, Ethiopia, North America|Europe|Asia|Africa|Oceania|United States|India|Brazil, New York|New Jersey, Europe|Portugal, Finland, Zhejiang Province, China, Europe|USA|Portugal|Germany|Australia|China|India|Pakistan|Sweden|Colorado, Malawi, Shanghai, Jordan, various countries|Global, China|global, Colombia|India|Spain|France|Greece|Germany|Argentina|Croatia|China|Brazil|Mexico|Romania|Philippines|United States, Ohio, USA, New Caledonia, Colombia|Chile|Argentina|Mexico|Brazil, Central Texas|Texas, Faisalabad, Pakistan, Northern Pacific Ocean|Norway Sea|Europe, Virginia, Maryland|California|New York|Pennsylvania, South Africa|Poland|Czech Republic|Romania|China|United States|Spain|Germany|France|Canada|Japan|Brazil|India|Croatia|Hungary|Slovenia|Slovakia|Czechia|Austria|Puerto Rico|Switzerland|Nigeria|Norway|Finland|Denmark|Sweden|Italy|South Korea|USA|UK|Australia|New Zealand|Mexico|Russia|Turkey|Egypt|Kenya|Ethiopia|Uganda|Tanzania|Malawi|Zambia|Zimbabwe|Mozambique|Madagascar|Comoros|Mayotte|Réunion|Guadeloupe|Martinique|Saint Martin|Saint Barthélemy|French Guiana|Suriname|Guyana|Argentina|Chile|Peru|Bolivia|Paraguay|Uruguay|Colombia|Venezuela|Ecuador|Costa Rica|Panama|Nicaragua|Honduras|El Salvador|Guatemala|Belize|Alaska|Hawaii|Maine|Massachusetts|New York|California|Texas|Florida|Illinois|Pennsylvania|Ohio|Georgia|North Carolina|Michigan|Virginia|Washington|Arizona|Colorado|Connecticut|Delaware|District of Columbia|Idaho|Indiana|Iowa|Kansas|Kentucky|Louisiana|Maryland|Minnesota|Mississippi|Missouri|Montana|Nebraska|Nevada|New Hampshire|New Jersey|New Mexico|North Dakota|Oklahoma|Oregon|Rhode Island|South Carolina|South Dakota|Tennessee|Utah|Vermont|West Virginia|Wisconsin|Wyoming, Ivory Coast|Ghana, Chongqing|Southwestern China, Portugal, Florida, Italy|Greece, USA|Europe|Asia|Africa|South America|North and South America, Palestine, Europe|Ukraine, Europe|North America, Kenya, Republic of Korea, Chile|Europe, USA|China|Japan|Spain|United Kingdom|Germany|France|South Korea|Netherlands|Viet Nam|Laos|Canada|Czech Republic|Argentina|Brazil|Belgium|Denmark|Iran|Australia|Croatia|Ghana|Mexico|Missing, United States|China|Colombia|Argentina|Chile|Australia|Singapore, Algeria, Europe|North America|Asia|Southern Europe|Western Asia, Croatia, Europe|North America|Asia|China, England, Northern Italy, Boston, USA|Vellore, India, Southwest Nigeria, Atlanta-metro area, Western Nepal, Calgary, Canada|Calgary, Alberta, Canada, Iraq, Vietnam, Europe|Pakistan, Peru, UK|Ireland, India|Sweden|China|Hong Kong, Central Adriatic Sea, Europe|Asia|North America|South America, Europe|Asia|North America, Romania | 2006, 2008, 2009, 2010, 2011, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021, 2022, 2023, 2024, 2025 | AF313472|X75562|AY008291|AF300985|Z21956|Z49084|Z30178|AF427133|AF227204|X77455 | - |
| blaKPC-5 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 8 | carbapenems, AZTREONAM +18 | Pseudomonas aeruginosa +2 | Puerto Rico, New Jersey, China | 2009, 2013, 2020, 2022 | EU400222 | ABY91240.1 |
| blaKPC-4 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 16 | AZTREONAM, MEROPENEM +21 | Klebsiella pneumoniae +8 | New Jersey, United States|Boston, MA, UK, United States|New York|Florida|Illinois|Gaza|Colombia|South America|Mediterranean region, South Korea, Boston, MA|Orange, CA, United States, various countries|Global, Ohio | 2009, 2012, 2013, 2015, 2016, 2018, 2020, 2022, 2023 | EU447304.1 | ACA34343.1 |
| blaKPC-7 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 5 | AZTREONAM, MEROPENEM +17 | Klebsiella pneumoniae +1 | - | 2009, 2010, 2015 | EU729727.1 | ACE62798.1 |
| blaKPC-10 | Card DatabaseReference Gene CatalogResFinder Database | 4 | AZTREONAM, MEROPENEM +15 | Acinetobacter baumannii | - | 2010 | GQ140348.1 | ACS35345.1 |
| blaKPC-8 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 8 | AZTREONAM, MEROPENEM +19 | Klebsiella pneumoniae +1 | Houston, Europe, Colombia | 2010, 2015, 2020, 2025 | FJ234412.1 | ACI95258.1 |
| blaKPC-11 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 7 | ertapenem, AZTREONAM +18 | Klebsiella pneumoniae | Greece|Spain|Taiwan|Turkey|Colombia|Puerto Rico|USA|Argentina|Chile|Germany|Italy|Philippines|Thailand, Italy|Greece, Asia|Latin America|Middle East|North America|South Pacific|Africa|Europe|South Africa|China | 2011, 2012, 2013 | HM066995 | ADH95186.1 |
| blaKPC-6 | Card DatabaseReference Gene CatalogResFinder Database | 4 | AZTREONAM, MEROPENEM +15 | Klebsiella pneumoniae | - | 2012 | EU555534.1 | ACB71165.1 |
| bla(kpc) | Reslit | 1 | ertapenem, imipenem | Klebsiella pneumoniae | Brazil | 2013 | - | - |
| blaKPC-2a | Reslit | 1 | aztreonam, ceftazidime +2 | Escherichia coli +1 | New York|France | 2013 | EU176011.1|EU176014.1 | - |
| blaKPC-2b | Reslit | 1 | aztreonam, ceftazidime +2 | Escherichia coli +1 | New York|France | 2013 | EU176011.1|EU176014.1 | - |
| blaKPC-2c | Reslit | 1 | aztreonam, ceftazidime +2 | Escherichia coli +1 | New York|France | 2013 | EU176011.1|EU176014.1 | - |
| blaKPC-15 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 5 | imipenem, meropenem +21 | Klebsiella pneumoniae +1 | China | 2014 | AY395881|JX500679|JX500680|X64523 | AGF70638.1 |
| blaKPC-17 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 6 | AZTREONAM, MEROPENEM +16 | Klebsiella pneumoniae | Taiwan | 2014, 2015, 2017 | KC465200.1 | AGJ01154.1 |
| blaKPC-13 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 6 | AZTREONAM, MEROPENEM +19 | Enterobacter cloacae +11 | Thailand, Middle East|Afghanistan|Iraq|Egypt|Pakistan|Lebanon|Iran|Jordan|Persian Gulf Countries|United Arab Emirates|Kuwait|Qatar|Bahrain|Oman|Saudi Arabia|Turkey | 2014, 2015 | HQ342889.1 | AEA73284.1 |
| blaKPC-16 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 5 | carbapenems, AZTREONAM +16 | Klebsiella pneumoniae | Taiwan | 2015 | KC465200|KC465199 | AGJ01153.1 |
| kpc | Reslit | 5 | carbapenems, imipenem +2 | Enterobacteriaceae +5 | Germany, China, Poland | 2015, 2018, 2021, 2023 | - | - |
| blaKPC-121 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Enterobacter cloacae | - | 2015 | OM933717.1 | UNN26053.1 |
| blaKPC-84 | Card DatabaseReference Gene CatalogReslit | 3 | CARBAPENEM, ceftazidime avibactam | Klebsiella pneumoniae +1 | China | 2015, 2024 | MW657985.1 | QSE38127.1 |
| blaKPC-18 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 7 | carbapenems, AZTREONAM +17 | Enterobacter cloacae +2 | Kentucky|United States, United States|Europe|Asia|Africa|South America|India|United Kingdom|Egypt|Belgium|Italy|China|United Arab Emirates, South Korea | 2016, 2021 | KT345945|KT345946|KP681699 | AKO63215.1 |
| blaKPC-133 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | 2016 | OP081531.1 | UUG60968.1 |
| blaKPC-24 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 6 | AZTREONAM, MEROPENEM +23 | Klebsiella pneumoniae +2 | Shanghai, China, China | 2016, 2022 | KR052099.1 | AKQ06274.1 |
| bla_KPC-2 | Reslit | 9 | carbapenems, cephalosporins +2 | Klebsiella pneumoniae +4 | Korea, Malaysia, Singapore, Vietnam, China, France|Spain, Wales, Northeast India|India | 2016, 2017, 2021, 2022, 2023, 2025 | pNDM-ECS01|pHS102707 | - |
| bla_KPC | Reslit | 13 | carbapenems, ertapenem +2 | Acinetobacter calcoaceticus-baumannii +5 | Europe|Brazil|China|Switzerland|Iran|Puerto Rico|United States, South Africa, France, Uganda, India, Veneto|Marche, Valencia, Spain, United Kingdom, China, Egypt, Croatia, Tianjin|Southern Karnataka, Brazil | 2016, 2018, 2021, 2022, 2023, 2024, 2025 | PRJNA850894 | - |
| blaKPC-32 | Card DatabaseReference Gene CatalogResFinder Database | 4 | AZTREONAM, MEROPENEM +15 | Klebsiella pneumoniae | - | 2017 | MAPO01000050.1 | OKL07573.1 |
| blaKPC-33 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 20 | AZTREONAM, MEROPENEM +23 | Klebsiella pneumoniae +2 | United States, China, Zhejiang Province, China, China|Poland, Northern Italy, Italy, China|Chile, Colombia, Europe | 2017, 2020, 2021, 2022, 2023, 2024, 2025, 2026 | CP025144.1 | AUD37084.1 |
| bla(KPC) | Reslit | 9 | carbapenems | Enterobacteriaceae +4 | Europe, China, Pakistan, Istanbul, Turkey | 2017, 2018, 2020, 2022, 2023, 2024, 2025 | CP025636.1|CP025468.1 | - |
| KPC | Reslit | 35 | carbapenems, beta lactams +13 | Escherichia coli +17 | South Korea, Europe|Texas, Tanzania|Thailand|human|swine, India, Argentina, Italy, Europe, Iran, Upper Egypt, China, Japan, Netherlands, Bangladesh, Sri Lanka, United States|Europe, United States|European Union, Guangzhou, China, Ho Chi Minh City, Vietnam, Port Harcourt, Nigeria, Brazil, New York City, Poland | 2017, 2018, 2019, 2020, 2021, 2022, 2023, 2024, 2025 | CP020847|CP020848|CP020849|CP020850|CP020851|CP020852 | - |
| blaKPC-19 | Card DatabaseReference Gene CatalogResFinder Database | 4 | AZTREONAM, MEROPENEM +15 | Klebsiella pneumoniae subsp. pneumoniae | - | 2018 | KJ775801.1 | AIH07017.1 |
| blaKPC-26 | Card DatabaseReference Gene CatalogResFinder Database | 4 | AZTREONAM, MEROPENEM +15 | Klebsiella pneumoniae | - | 2017 | KX619622.1 | ANY26940.1 |
| blaKPC-34 | Card DatabaseReference Gene CatalogResFinder Database | 4 | AZTREONAM, MEROPENEM +15 | Klebsiella pneumoniae | - | 2018 | KU985429.1 | AMS25623.1 |
| bla(KPC-2) | Reslit | 32 | carbapenems, ceftazidime avibactam +12 | Enterobacter cloacae +17 | Brazil, Colombia, China, China|United States, Henan, China, US, Shenzhen, China, Southwestern China, Chile, Greece, Europe, Europe|North America|Asia|China, Europe|Switzerland, Western China | 2018, 2019, 2020, 2021, 2022, 2023, 2024, 2025, 2026 | NCBI:MH051105 | - |
| blaKPC-2_1 | Reslit | 1 | carbapenems | Klebsiella pneumoniae | Australia | 2019 | AY034847|AF192329|CP002151|V00359|EU370913|AJ427422|JF703130|FJ158002|AB571791|KF864551|NZ_ACWO01000079|NC_021994.1|CP008797 | - |
| blaKPC-35 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 7 | CEFIDEROCOL, AZTREONAM +18 | Klebsiella pneumoniae +1 | China, Peru|Spain | 2019, 2021, 2024 | MH404098.1 | AWR93230.1 |
| bla_kpc-2 | Reslit | 3 | imipenem, meropenem +2 | Klebsiella pneumoniae | China, South China | 2019, 2020, 2023 | - | - |
| blaKPC-14 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 11 | AZTREONAM, MEROPENEM +22 | Klebsiella pneumoniae +1 | Houston, Hanoi, Vietnam|Vietnam, China, Germany, Italy, Europe | 2019, 2020, 2021, 2023, 2024, 2025, 2026 | JX524191.1 | AFV48348.1 |
| blaKPC-28 | Card DatabaseReference Gene CatalogResFinder Database | 4 | AZTREONAM, MEROPENEM +15 | Escherichia coli | - | 2019 | KY282958.1 | APG42690.1 |
| blaKPC-40 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 5 | ceftazidime avibactam, AZTREONAM +17 | Enterobacter hormaechei +1 | United States | 2019 | QRBQ00000000.1|QRBO00000000.1|QRBS00000000.1|QRBR00000000.1|MK862125 | RDT05676.1 |
| blaKPC-39 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 7 | AZTREONAM, MEROPENEM +17 | Klebsiella pneumoniae +2 | Spain, Italy, France | 2019, 2020, 2021 | MK118771 | AYR04937.1 |
| blaKPC-41 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 6 | ceftazidime avibactam, AZTREONAM +18 | Klebsiella pneumoniae +1 | Switzerland | 2019, 2023 | MK497255 | QBC36180.1 |
| blaKPC-36 | Card DatabaseReference Gene CatalogResFinder Database | 4 | AZTREONAM, MEROPENEM +16 | Klebsiella pneumoniae | - | 2020 | MH593787.1 | AXC08545.1 |
| blaKPC-48 | Card DatabaseReference Gene CatalogReslit | 3 | ceftazidime avibactam, CEPHALOSPORIN | Klebsiella pneumoniae | Spain | 2020 | MN422013.1 | QEO76002.1 |
| blaKPC-50 | Card DatabaseReference Gene CatalogReslit | 3 | ceftazidime avibactam, CEPHALOSPORIN | Klebsiella pneumoniae +1 | Switzerland|Greece | 2020 | MN654342 | QGJ02578.1 |
| blaKPC-like | Reslit | 5 | carbapenems, imipenem +3 | Klebsiella pneumoniae +3 | Brazil, Norway, Taiwan, China | 2020, 2021, 2024, 2026 | CP042878-CP042881|CP042882-CP042884|CP042885-CP042892|CP044402-CP044406|CP047609-CP047613|CP043406-CP043413|JAAAYF000000000|JAAAYE000000000 | - |
| blaKPC–2 | Reslit | 8 | ceftazidime avibactam, carbapenems +14 | Klebsiella pneumoniae +4 | China, China|Henan province, China, Europe | 2020, 2021, 2022, 2025 | cp054720|cp054721|cp054722|cp054723|cp054724|cp054725|cp054726|cp054727|cp054728|cp054729|cp054730|cp054731|cp054732|cp054733|cp054734|cp054735|cp054736|cp054737|cp054738|cp054739|cp054740|cp054741|cp054742|cp054743|cp054744|cp054745|cp054746|cp054747|cp054748|cp054749|cp054750|cp054751|cp054752|cp054753|cp054754|cp054755|cp054756|cp054757|cp054758|cp054759|cp054760|cp054761|cp054762|cp054763|cp054764|cp054765|cp054766|cp054767|cp054768|cp054769|cp054770|cp054771|cp054772|cp054773|cp054774|cp054775|cp054776|cp054777|cp054778|cp054779|cp054780|cp054781|cp054782|cp054783|cp054784|cp054785 | - |
| blaKPC-57 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae subsp. pneumoniae | - | 2021 | MT358626.1 | QJD09264.1 |
| bla(KPC-14) | Reslit | 1 | ceftazidime avibactam | Klebsiella pneumoniae +1 | New York City | 2020 | CP045015|CP045016|CP045017|CP045018|CP045019|CP045020|CP045021|CP045022 | - |
| blaKPC– 2 | Reslit | 1 | meropenem, ertapenem +1 | Escherichia coli | Germany | 2020 | - | - |
| blaKPC-53 | Card DatabaseReference Gene CatalogReslit | 3 | ceftazidime avibactam, CEPHALOSPORIN | Klebsiella pneumoniae +1 | Italy | 2020 | MUT87649.1 | QOS10027.1 |
| blaKPC-55 | Card DatabaseReference Gene CatalogReslit | 3 | aztreonam, meropenem +1 | Klebsiella pneumoniae +1 | South Korea | 2020 | MT028409 | QIS31290.1 |
| blaKPC-49 | Card DatabaseReference Gene CatalogReslit | 6 | ceftazidime avibactam, CEPHALOSPORIN +1 | Escherichia coli +1 | Spain, Italy | 2021, 2023, 2024 | MN619655|WIRF00000000|WIRG00000000 | QFX75995.1 |
| blaKPC-12 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 8 | AZTREONAM, MEROPENEM +17 | Klebsiella pneumoniae +1 | Eastern Europe|Western Europe, Hanoi, Vietnam|Vietnam, China | 2021, 2024 | HQ641421.1 | ADZ75467.1 |
| blaKPC-30 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 5 | AZTREONAM, MEROPENEM +16 | Klebsiella pneumoniae | Brazil | 2021, 2025 | KY646302.1 | AQT03460.1 |
| blaKPC-31 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 20 | CEFIDEROCOL, AZTREONAM +19 | Klebsiella pneumoniae +10 | Italy, North Carolina, Southern Italy, Argentina, Sardinia, Italy, Colombia, Brazil | 2021, 2022, 2023, 2024, 2025 | MAPH01000113.1 | OKK72649.1 |
| blaKPC-51 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae | - | 2021 | MN725731.1 | QGT31448.1 |
| blaKPC-52 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae | - | 2021 | MN725732.1 | QGT31449.1 |
| bla(KPC-33) | Reslit | 3 | ceftazidime avibactam | Klebsiella pneumoniae +1 | Henan, China, Chile, Europe | 2021, 2022, 2024 | CP059105|CP059106 | - |
| blaKPC-29 | Card DatabaseReference Gene CatalogResFinder DatabaseReslit | 6 | ceftazidime avibactam, AZTREONAM +27 | Klebsiella pneumoniae | Italy, Kuwait | 2021, 2022 | MT809692|MT809694|MT809695|MT809697|MT809698|MT809699|MT809700|MT809701|MT809702|MW650887|MW650888|MW650889|MW650890|MW650891|MW650892|MT809688|MT809693|MT809689|MT809691|MT809690|MT809696 | AQM40193.1 |
| blaKPC-67 | Card DatabaseReference Gene CatalogReslit | 3 | ceftazidime avibactam, CARBAPENEM | Klebsiella pneumoniae | Italy | 2021 | MT809692|MT809694|MT809695|MT809697|MT809698|MT809699|MT809700|MT809701|MT809702|MW650887|MW650888|MW650889|MW650890|MW650891|MW650892|MT809688|MT809693|MT809689|MT809691|MT809690|MT809696 | QMU31921.1 |
| blaKPC-68 | Card DatabaseReference Gene CatalogReslit | 3 | ceftazidime avibactam, CEPHALOSPORIN | Klebsiella pneumoniae | Italy | 2021 | MT809692|MT809694|MT809695|MT809697|MT809698|MT809699|MT809700|MT809701|MT809702|MW650887|MW650888|MW650889|MW650890|MW650891|MW650892|MT809688|MT809693|MT809689|MT809691|MT809690|MT809696 | QMU31938.1 |
| blaKPC-69 | Card DatabaseReference Gene CatalogReslit | 3 | ceftazidime avibactam, CEPHALOSPORIN | Klebsiella pneumoniae | Italy | 2021 | MT809692|MT809694|MT809695|MT809697|MT809698|MT809699|MT809700|MT809701|MT809702|MW650887|MW650888|MW650889|MW650890|MW650891|MW650892|MT809688|MT809693|MT809689|MT809691|MT809690|MT809696 | QMU32012.1 |
| blaKPC-70 | Card DatabaseReference Gene CatalogReslit | 5 | ceftazidime avibactam, CEPHALOSPORIN +7 | Klebsiella pneumoniae +1 | Italy, Portugal | 2021, 2022, 2023 | MT809692|MT809694|MT809695|MT809697|MT809698|MT809699|MT809700|MT809701|MT809702|MW650887|MW650888|MW650889|MW650890|MW650891|MW650892|MT809688|MT809693|MT809689|MT809691|MT809690|MT809696 | QMU32028.1 |
| blaKPC-264 | Reference Gene Catalog | 1 | CEPHALOSPORIN | Klebsiella pneumoniae | - | 2021 | JBDKWS010000005.1 | MEN6698983.1 |
| blaKPC-2/3 | Reslit | 1 | imipenem, meropenem +1 | Escherichia coli +1 | Northern Portugal | 2021 | - | - |
| bla_KPC-3 | Reslit | 2 | carbapenems, meropenem vaborbactam +2 | Escherichia coli +1 | France|Spain, Italy | 2022, 2023 | NCBI:NC_000913.3|NCBI:NC_011740.1|EnteroBase|GCA_005886035.1|CNR65D6|GCA_004759025.1|CNR85I8|OU701452.1|OU701449.1 | - |
| blaKPC-94 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae | - | 2022 | MZ646140.1 | QYE52346.1 |
| blaKPC-95 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae | - | 2022 | MZ646141.1 | QYE52347.1 |
| blaKPC-46 | Card DatabaseReference Gene CatalogReslit | 3 | ceftazidime avibactam, CEPHALOSPORIN | Klebsiella pneumoniae | Spain | 2022 | JAHUYV000000000|JAHUZA000000000|MZ461464 | QED08959.1 |
| blaKPC-92 | Card DatabaseReference Gene CatalogReslit | 3 | ceftazidime avibactam, CEPHALOSPORIN | Klebsiella pneumoniae | Spain | 2022 | JAHUYV000000000|JAHUZA000000000|MZ461464 | QXG83132.1 |
| blaKPC-47 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae | - | 2022 | MN422012.1 | QEO76001.1 |
| blaKPC-93 | Card DatabaseReference Gene CatalogReslit | 4 | ceftazidime avibactam, CEPHALOSPORIN | Klebsiella pneumoniae +1 | China, Zhejiang Province, China | 2022 | MZ569034.1 | QYZ75849.1 |
| bla(KPC)-2 | Reslit | 1 | carbapenems | Klebsiella pneumoniae | China | 2022 | - | - |
| blaKPC-123 | Card DatabaseReference Gene CatalogReslit | 3 | ceftazidime, ceftazidime avibactam +1 | Citrobacter koseri +1 | China | 2022 | ON209376 | UNN26644.1 |
| blaKPC-114 | Card DatabaseReference Gene CatalogReslit | 3 | CARBAPENEM, ceftazidime avibactam | Klebsiella pneumoniae +1 | South America | 2022, 2023 | OM728507.1 | ULU82277.1 |
| blaKPC-104 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae subsp. pneumoniae | - | 2022 | OL445424.1 | UFK32671.1 |
| blaKPC-106 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae subsp. pneumoniae | - | 2022 | OL445428.1 | UFK32675.1 |
| blaKPC-107 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae subsp. pneumoniae | - | 2022 | OL445425.1 | UFK32672.1 |
| blaKPC-108 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae subsp. pneumoniae | - | 2022 | OL445427.1 | UFK32674.1 |
| blaKPC-96 | Card DatabaseReference Gene CatalogReslit | 3 | CARBAPENEM, ceftazidime +1 | Klebsiella pneumoniae +1 | Argentina | 2022, 2024 | OK086970.1 | UAY85936.1 |
| blaKPC-98 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | 2022 | MZ893466.1 | UAZ57792.1 |
| blaKPC-86 | Card DatabaseReference Gene CatalogReslit | 4 | CEPHALOSPORIN, ceftazidime avibactam | Klebsiella pneumoniae +1 | China, Europe | 2022, 2024, 2026 | MZ067229.1 | QUR41142.1 |
| blaKPC-87 | Card DatabaseReference Gene CatalogReslit | 3 | CARBAPENEM, ceftazidime avibactam | Klebsiella pneumoniae +1 | China | 2022, 2025 | MZ067230.1 | QUR41143.1 |
| blaKPC-88 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae | - | 2022 | MZ067231.1 | QUR41144.1 |
| blaKPC-113 | Card DatabaseReference Gene CatalogReslit | 3 | CARBAPENEM, ceftazidime avibactam | Klebsiella pneumoniae +1 | South America | 2023 | OM728506.1 | ULU82276.1 |
| blaKPC-112 | Card DatabaseReference Gene CatalogReslit | 3 | ceftazidime avibactam, CEPHALOSPORIN | Klebsiella pneumoniae +1 | China | 2022 | CP107245 | UIY90578.1 |
| bla_KPC-66 | Reslit | 1 | meropenem vaborbactam, imipenem relebactam +1 | Klebsiella pneumoniae | Italy | 2023 | CCEY01000001 | - |
| bla_KPC-68 | Reslit | 1 | meropenem vaborbactam, imipenem relebactam +1 | Klebsiella pneumoniae | Italy | 2023 | CCEY01000001 | - |
| bla_KPC-31 | Reslit | 1 | meropenem vaborbactam, imipenem relebactam +1 | Klebsiella pneumoniae | Italy | 2023 | CCEY01000001 | - |
| bla_KPC-121 | Reslit | 1 | meropenem vaborbactam, imipenem relebactam +1 | Klebsiella pneumoniae | Italy | 2023 | CCEY01000001 | - |
| bla_KPC-125 | Reslit | 1 | meropenem vaborbactam, imipenem relebactam +1 | Klebsiella pneumoniae | Italy | 2023 | CCEY01000001 | - |
| blaKPC-145 | Card DatabaseReference Gene CatalogReslit | 4 | ceftazidime avibactam, CEPHALOSPORIN +1 | Klebsiella pneumoniae | China | 2023, 2025 | OP626310 | UYF23564.1 |
| blaKPC-134 | Card DatabaseReference Gene CatalogReslit | 3 | ceftazidime avibactam, ceftazidime +3 | Klebsiella pneumoniae +1 | China | 2023, 2025 | OP293349.1 | UVJ69199.1 |
| blaKPC-154 | Card DatabaseReference Gene CatalogReslit | 3 | ceftazidime avibactam, CEPHALOSPORIN | Klebsiella pneumoniae +2 | Italy | 2023 | OQ096263|OQ282880|OQ282881 | WAY00162.1 |
| bla(KPC-80) | Reslit | 1 | ceftazidime, ceftazidime avibactam +1 | Klebsiella pneumoniae +1 | Argentina | 2024 | NCBI:CP079825|NCBI:CP079826|NCBI:CP079827|NCBI:CP079828 | - |
| bla(KPC-81) | Reslit | 1 | ceftazidime, ceftazidime avibactam +1 | Klebsiella pneumoniae +1 | Argentina | 2024 | NCBI:CP079825|NCBI:CP079826|NCBI:CP079827|NCBI:CP079828 | - |
| bla(KPC-96) | Reslit | 1 | ceftazidime, ceftazidime avibactam +1 | Klebsiella pneumoniae +1 | Argentina | 2024 | NCBI:CP079825|NCBI:CP079826|NCBI:CP079827|NCBI:CP079828 | - |
| bla(KPC-97) | Reslit | 1 | ceftazidime, ceftazidime avibactam +1 | Klebsiella pneumoniae +1 | Argentina | 2024 | NCBI:CP079825|NCBI:CP079826|NCBI:CP079827|NCBI:CP079828 | - |
| blaKPC -like | Reslit | 1 | ertapenem, meropenem +1 | Acinetobacter baumannii +14 | Southwest Amazon region|Rondônia|Brazil | 2024 | - | - |
| blaKPC-197 | Card DatabaseReference Gene CatalogReslit | 3 | ceftazidime avibactam, CEPHALOSPORIN | Klebsiella pneumoniae | Colombia | 2024 | OR633287 | WOE87882.1 |
| blaKPC-158 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | 2024 | OQ305823.1 | WDE35082.1 |
| blaKPC-168 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | 2024 | OR568564.1 | WNH41911.1 |
| blaKPC-169 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae subsp. pneumoniae | - | 2024 | OQ856767.1 | WGW19922.1 |
| blaKPC-170 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae | - | 2024 | OR449906.1 | WMP15087.1 |
| blaKPC-171 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae subsp. pneumoniae | - | 2024 | OQ856769.1 | WGW19924.1 |
| blaKPC-172 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae subsp. pneumoniae | - | 2024 | OQ856771.1 | WGW19926.1 |
| blaKPC-173 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae subsp. pneumoniae | - | 2024 | OQ856772.1 | WGW19927.1 |
| blaKPC-174 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae subsp. pneumoniae | - | 2024 | OQ856773.1 | WGW19928.1 |
| blaKPC-175 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae subsp. pneumoniae | - | 2024 | OQ856774.1 | WGW19929.1 |
| blaKPC-176 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | 2024 | OR687591.1 | WOE87885.1 |
| blaKPC-177 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae subsp. pneumoniae | - | 2024 | OQ856765.1 | WGW19920.1 |
| bla(KPC-203) | Reslit | 1 | ceftazidime avibactam | Klebsiella pneumoniae | Italy | 2024 | CP099005 | - |
| blaKPC-203 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae | - | 2024 | JAXHPW010000039.1 | MDY6716274.1 |
| blaKPC-216 | Card DatabaseReference Gene CatalogReslit | 3 | ceftazidime avibactam, CARBAPENEM | Klebsiella pneumoniae ST101 +1 | Italy | 2024 | MT809698.1|MT809692.1 | WWO49380.1 |
| blaKPC - 2 | Reslit | 1 | carbapenems | Klebsiella pneumoniae | China | 2024 | PRJNA786277 | - |
| blaKPC-189 | Card DatabaseReference Gene CatalogReslit | 3 | ceftazidime avibactam, CEPHALOSPORIN | Klebsiella pneumoniae +1 | China | 2024 | JBCGZU000000000|JBAGBV000000000|CP144138|CP144139|CP144140|CP144141|OR501577 | WMQ58826.1 |
| bla(KPC-3) | Reslit | 2 | carbapenems | Escherichia coli ST-361 +1 | Germany|Ukraine, Europe | 2024, 2025 | PMC11539215 | - |
| blaKPC-3 D179N | Reslit | 1 | ceftazidime avibactam | Escherichia coli | - | 2024 | - | - |
| blaKPC-2 L169A | Reslit | 1 | ceftazidime avibactam | Klebsiella pneumoniae | - | 2024 | - | - |
| blaKPC-2 L169T | Reslit | 1 | ceftazidime avibactam | Klebsiella pneumoniae | - | 2024 | - | - |
| blaKPC-179 | Card DatabaseReference Gene CatalogReslit | 4 | ceftazidime avibactam, CEPHALOSPORIN | Klebsiella pneumoniae HX1192 +3 | Europe | 2024, 2025 | CP098123|CP098124|CP098125 | WIU89430.1 |
| blaKPC-27 | Card DatabaseReference Gene CatalogResFinder Database | 4 | AZTREONAM, MEROPENEM +16 | Klebsiella pneumoniae | - | 2025 | KX828722.1 | AOR05747.1 |
| blaKPC-167 | Card DatabaseReference Gene Catalog | 2 | CEPHALOSPORIN | Klebsiella pneumoniae | - | 2025 | OQ592370.1 | WEG44934.1 |
| blaKPC-205 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | 2025 | JAYEEW010000028.1 | MEA1860518.1 |
| blaKPC-163 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | 2025 | OQ579139.1 | WEG44277.1 |
| blaKPC-224 | Card DatabaseReference Gene Catalog | 2 | CARBAPENEM | Klebsiella pneumoniae | - | 2025 | PP728767.1 | XAM88516.1 |
| blaKPC-276 | Reference Gene Catalog | 1 | CARBAPENEM | Klebsiella pneumoniae | - | 2025 | MAPK01000127.1 | OKK87301.1 |
| bla(KPC-71) | Reslit | 1 | ceftazidime avibactam | Klebsiella pneumoniae +1 | China|Jiangxi, China | 2025 | - | - |
| bla(KPC-263) | Reslit | 1 | ceftazidime avibactam | Klebsiella pneumoniae | China | 2025 | PV219192 | - |
| bla(KPC-90) | Reslit | 1 | ceftazidime avibactam | Klebsiella pneumoniae | China | 2025 | PV219192 | - |
| bla_kpc-1 | Reslit | 1 | imipenem, meropenem | Klebsiella pneumoniae | Europe|China | 2025 | PRJNA1283480 | - |
Novel carbapenem-hydrolyzing beta-lactamase, KPC-1, from a carbapenem-resistant strain of Klebsiella pneumoniae.
The study identifies KPC-1, a novel class A carbapenem-hydrolyzing beta-lactamase, as the primary cause of carbapenem resistance in a Klebsiella pneumoniae strain. The gene was cloned and shown to confer resistance to multiple beta-lactam antibiotics.
Occurrence of newer beta-lactamases in Klebsiella pneumoniae isolates from 24 U.S. hospitals.
The study identified several beta-lactamases, including bla DHA-1, bla ACT-1, bla FOX-5, bla CMY-2, and bla KPC-2, in Klebsiella pneumoniae isolates from 24 U.S. hospitals. These enzymes confer resistance to various beta-lactam antibiotics.
Plasmid-mediated, carbapenem-hydrolysing beta-lactamase, KPC-2, in Klebsiella pneumoniae isolates.
Plasmid-mediated, carbapenem-hydrolysing beta-lactamase, KPC-2, in Klebsiella pneumoniae isolates.
Plasmid-mediated, carbapenem-hydrolysing beta-lactamase, KPC-2, in Klebsiella pneumoniae isolates.
Plasmid-mediated, carbapenem-hydrolysing beta-lactamase, KPC-2, in Klebsiella pneumoniae isolates., Detection of KPC-2 in a clinical isolate of Proteus mirabilis and first reported description of carbapenemase resistance caused by a KPC beta-lactamase in P. mirabilis.
Imipenem resistance in a Salmonella clinical strain due to plasmid-mediated class A carbapenemase KPC-2.
The study identifies KPC-2, a plasmid-mediated class A carbapenemase, as the cause of imipenem resistance in a Salmonella enterica serotype Cubana isolate.
Carbapenem-resistant strain of Klebsiella oxytoca harboring carbapenem-hydrolyzing beta-lactamase KPC-2.
The study identifies KPC-2, a class A carbapenem-hydrolyzing beta-lactamase, as the primary mechanism of carbapenem resistance in a clinical isolate of Klebsiella oxytoca. KPC-2 was shown to confer resistance to carbapenems, extended-spectrum cephalosporins, and aztreonam when cloned in E. coli.
Outbreak of Klebsiella pneumoniae producing a new carbapenem-hydrolyzing class A beta-lactamase, KPC-3, in a New York Medical Center.
The study identifies a new carbapenem-hydrolyzing class A beta-lactamase, KPC-3, produced by carbapenem-resistant Klebsiella pneumoniae isolates. The enzyme was encoded on a 75-kb plasmid and conferred resistance to carbapenems, cephalosporins, and other beta-lactam antibiotics.
Outbreak of Klebsiella pneumoniae producing a new carbapenem-hydrolyzing class A beta-lactamase, KPC-3, in a New York Medical Center.
Outbreak of Klebsiella pneumoniae producing a new carbapenem-hydrolyzing class A beta-lactamase, KPC-3, in a New York Medical Center.
Outbreak of Klebsiella pneumoniae producing a new carbapenem-hydrolyzing class A beta-lactamase, KPC-3, in a New York Medical Center.
Evaluation of techniques for detection of carbapenem-resistant Klebsiella pneumoniae in stool surveillance cultures.
The study evaluates techniques for detecting carbapenem-resistant Klebsiella pneumoniae, highlighting the role of the bla KPC-2 gene in conferring resistance to carbapenems.
Plasmid-mediated imipenem-hydrolyzing enzyme KPC-2 among multiple carbapenem-resistant Escherichia coli clones in Israel.
The study reports the first discovery of the plasmid-mediated carbapenem-hydrolyzing enzyme KPC-2 in Escherichia coli in Israel, highlighting its role in carbapenem resistance among multiple genetically unrelated clones.
Prevalence of newer beta-lactamases in gram-negative clinical isolates collected in the United States from 2001 to 2002.
The study identified various beta-lactamases including SHV, CTX-M, FOX, CMY, DHA, and KPC, which confer resistance to multiple beta-lactam antibiotics in gram-negative clinical isolates in the United States.
Carbapenem resistance in Klebsiella pneumoniae not detected by automated susceptibility testing.
The study identifies blaKPC-1, blaKPC-2, and blaKPC-3 genes as responsible for carbapenem resistance in Klebsiella pneumoniae isolates, highlighting challenges in detecting this resistance through automated susceptibility testing systems.
Carbapenem resistance in Klebsiella pneumoniae not detected by automated susceptibility testing.
The study identifies blaKPC-1, blaKPC-2, and blaKPC-3 genes as responsible for carbapenem resistance in Klebsiella pneumoniae isolates, highlighting challenges in detecting this resistance through automated susceptibility testing systems.
Carbapenem resistance in Klebsiella pneumoniae not detected by automated susceptibility testing.
The study identifies blaKPC-1, blaKPC-2, and blaKPC-3 genes as responsible for carbapenem resistance in Klebsiella pneumoniae isolates, highlighting challenges in detecting this resistance through automated susceptibility testing systems.
Crystal structure of KPC-2: insights into carbapenemase activity in Class A beta-lactamases.
The study presents the crystal structure of KPC-2, a clinically important carbapenemase, and provides insights into its carbapenemase activity, highlighting its ability to hydrolyze carbapenems and cephamycins.
Genetic structures at the origin of acquisition of the beta-lactamase bla KPC gene.
The study characterizes the genetic structures surrounding the bla KPC-2 gene, identifying a novel transposon Tn 4401 that facilitates the acquisition and dissemination of the gene.
Isolation of imipenem-resistant Enterobacter species: emergence of KPC-2 carbapenemase, molecular characterization, epidemiology, and outcomes.
The study identifies the KPC-2 carbapenemase as a key factor in imipenem resistance among Enterobacter species, highlighting its role in reducing susceptibility to carbapenem antibiotics.
High-level carbapenem resistance in a Citrobacter freundii clinical isolate is due to a combination of KPC-2 production and decreased porin expression.
The study identified that high-level carbapenem resistance in a Citrobacter freundii clinical isolate is due to the production of KPC-2 beta-lactamase and decreased porin expression.
Emergence of Serratia marcescens, Klebsiella pneumoniae, and Escherichia coli Isolates possessing the plasmid-mediated carbapenem-hydrolyzing beta-lactamase KPC-2 in intensive care units of a Chinese hospital.
The study identifies the plasmid-mediated carbapenem-hydrolyzing beta-lactamase KPC-2 in Serratia marcescens, Klebsiella pneumoniae, and Escherichia coli isolates from a Chinese hospital, highlighting its role in carbapenem resistance.
Klebsiella pneumoniae Carbapenemase-2, Buenos Aires, Argentina.
The study identifies KPC-2, a carbapenemase, in two clinical isolates of Klebsiella pneumoniae and Citrobacter freundii in Argentina. The presence of KPC-2 was confirmed through PCR, sequencing, and enzymatic activity assays.
Rapid Detection of blaKPC Carbapenemase Genes by Real-Time PCR
The study developed and validated a real-time PCR assay for detecting blaKPC genes, which are responsible for carbapenem resistance in Klebsiella pneumoniae.
Genetic and structural insights into the dissemination potential of the extremely broad-spectrum class A beta-lactamase KPC-2 identified in an Escherichia coli strain and an Enterobacter cloacae strain isolated from the same patient in France.
The study identifies and characterizes the KPC-2 beta-lactamase, a broad-spectrum class A enzyme responsible for resistance to carbapenems and cephalosporins in Escherichia coli and Enterobacter cloacae strains isolated from the same patient in France.
Prevalence and mechanisms of broad-spectrum beta-lactam resistance in Enterobacteriaceae: a children's hospital experience.
The study identifies multiple beta-lactamase genes, including bla CMY-2, bla DHA, bla TEM, bla CTX-M, bla IMP-4, and bla KPC-2, contributing to broad-spectrum beta-lactam resistance in Enterobacteriaceae isolates from a children's hospital.
Phenotypic and enzymatic comparative analysis of the novel KPC variant KPC-5 and its evolutionary variants, KPC-2 and KPC-4.
The study characterizes the novel KPC variant KPC-5, which differs from KPC-2 by a single amino acid substitution (Pro 103 → Arg) and from KPC-4 by an additional amino acid change (Val 239 → Gly). KPC-5 shows distinct enzymatic properties compared to KPC-2 and KPC-4, including differences in isoelectric point and susceptibility to clavulanic acid inhibition.
Phenotypic and enzymatic comparative analysis of the novel KPC variant KPC-5 and its evolutionary variants, KPC-2 and KPC-4.
Phenotypic and enzymatic comparative analysis of the novel KPC variant KPC-5 and its evolutionary variants, KPC-2 and KPC-4.
Phenotypic and enzymatic comparative analysis of the novel KPC variant KPC-5 and its evolutionary variants, KPC-2 and KPC-4.
Phenotypic and enzymatic comparative analysis of the novel KPC variant KPC-5 and its evolutionary variants, KPC-2 and KPC-4.
Phenotypic and enzymatic comparative analysis of the novel KPC variant KPC-5 and its evolutionary variants, KPC-2 and KPC-4.
Phenotypic and enzymatic comparative analysis of the novel KPC variant KPC-5 and its evolutionary variants, KPC-2 and KPC-4.
Phenotypic and enzymatic comparative analysis of the novel KPC variant KPC-5 and its evolutionary variants, KPC-2 and KPC-4.
Phenotypic and enzymatic comparative analysis of the novel KPC variant KPC-5 and its evolutionary variants, KPC-2 and KPC-4.
Characterization and sequence analysis of extended-spectrum-{beta}-lactamase-encoding genes from Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis isolates collected during tigecycline phase 3 clinical trials.
The study identified various extended-spectrum beta-lactamase (ESBL) genes, including bla CTX-M-1, bla CTX-M-2, bla CTX-M-5, bla CTX-M-15, bla CTX-M-22, bla CTX-M-28, bla SHV-5, bla SHV-12, bla TEM-1, bla OXA-1, bla OXA-2, bla OXA-5, bla PER-1, bla ACT-1, bla MIR-1, bla CMY-2, bla FOX-1, bla DHA-1, and bla KPC-2, in Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis isolates.
Development and evaluation of a real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase genes.
The study developed a real-time PCR assay to detect and differentiate blaKPC genes, which encode KPC carbapenemases responsible for carbapenem resistance in Klebsiella pneumoniae and Klebsiella oxytoca.
Development and evaluation of a real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase genes.
Development and evaluation of a real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase genes.
Development and evaluation of a real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase genes.
Characterization of blaKPC-containing Klebsiella pneumoniae isolates detected in different institutions in the Eastern USA.
The study characterizes blaKPC-containing Klebsiella pneumoniae isolates, identifying blaKPC-2 and blaKPC-3 as the primary carbapenemase genes, along with blaTEM-1, blaSHV-11, and blaSHV-12 as common beta-lactamase genes, highlighting their role in multidrug resistance.
Characterization of blaKPC-containing Klebsiella pneumoniae isolates detected in different institutions in the Eastern USA.
The study characterizes blaKPC-containing Klebsiella pneumoniae isolates, identifying blaKPC-2 and blaKPC-3 as the primary carbapenemase genes, along with blaTEM-1, blaSHV-11, and blaSHV-12 as common beta-lactamase genes, highlighting their role in multidrug resistance.
Fatal cross infection by carbapenem-resistant Klebsiella in two liver transplant recipients.
The study identifies the blaKPC-2 gene as responsible for carbapenem resistance in Klebsiella isolates from two liver transplant recipients, highlighting the challenges in diagnosing and treating carbapenem-resistant Enterobacteriaceae infections.
Genetic organization of transposase regions surrounding blaKPC carbapenemase genes on plasmids from Klebsiella strains isolated in a New York City hospital.
The study characterizes the genetic organization of blaKPC carbapenemase genes on plasmids from Klebsiella strains, identifying two isoforms of Tn4401 and demonstrating the presence of blaKPC-2 and blaKPC-3 alleles.
Detection of plasmid-mediated KPC-producing Klebsiella pneumoniae in Ottawa, Canada: evidence of intrahospital transmission.
The study reports the first three cases of KPC-producing K. pneumoniae in Canada and identifies the blaKPC gene as responsible for carbapenem resistance in these isolates.
Performance of the Phoenix bacterial identification system compared with disc diffusion methods for identifying extended-spectrum beta-lactamase, AmpC and KPC producers.
The study highlights the limitations of the Phoenix system in accurately detecting ESBLs, AmpC, and KPC producers, emphasizing the need for confirmatory methods. It identifies bla KPC as a gene conferring resistance to carbapenems.
In vitro activity of the {beta}-lactamase inhibitor NXL104 against KPC-2 carbapenemase and Enterobacteriaceae expressing KPC carbapenemases.
NXL104 effectively inhibits KPC-2 carbapenemase, restoring the activity of beta-lactam antibiotics against Enterobacteriaceae producing KPC enzymes.
Emergence of KPC-producing Pseudomonas aeruginosa in Trinidad and Tobago.
The study reports the emergence of KPC-2-producing Pseudomonas aeruginosa in Trinidad and Tobago, highlighting its pan-resistance and the challenges in detection and treatment.
Novel genetic environment of the carbapenem-hydrolyzing beta-lactamase KPC-2 among Enterobacteriaceae in China.
The study characterizes the novel genetic environment of the blaKPC-2 gene in Enterobacteriaceae isolates from China, identifying a unique integration structure involving a Tn3-based transposon and a partial Tn4401 segment.
Evaluation of PCR-based testing for surveillance of KPC-producing carbapenem-resistant members of the Enterobacteriaceae family.
The study evaluates a PCR-based method for detecting KPC-producing carbapenem-resistant Enterobacteriaceae, demonstrating high sensitivity and specificity compared to culture-based methods.
Emergence of blaKPC-containing Klebsiella pneumoniae in a long-term acute care hospital: a new challenge to our healthcare system.
The study identifies blaKPC as a gene conferring resistance to carbapenems in Klebsiella pneumoniae isolates from a long-term acute care hospital, highlighting the emergence of this resistance mechanism in a new clinical setting.
First descriptions of blaKPC in Raoultella spp. (R. planticola and R. ornithinolytica): report from the SENTRY Antimicrobial Surveillance Program.
The study reports the first descriptions of blaKPC in Raoultella spp., specifically R. planticola and R. ornithinolytica, highlighting their carbapenem resistance mechanisms.
First descriptions of blaKPC in Raoultella spp. (R. planticola and R. ornithinolytica): report from the SENTRY Antimicrobial Surveillance Program.
The study reports the first descriptions of blaKPC in Raoultella spp., specifically R. planticola and R. ornithinolytica, highlighting their carbapenem resistance mechanisms.
Updated functional classification of beta-lactamases.
The paper updates the functional classification of beta-lactamases, detailing their substrate preferences, inhibitor profiles, and clinical relevance. It highlights the diversity and evolution of these enzymes, emphasizing their role in antibiotic resistance.
Inhibitor resistance in the KPC-2 beta-lactamase, a preeminent property of this class A beta-lactamase.
The KPC-2 beta-lactamase exhibits resistance to beta-lactamase inhibitors such as clavulanic acid, sulbactam, and tazobactam. It hydrolyzes these inhibitors efficiently, which contributes to its resistance properties.
Detection of KPC in Acinetobacter spp. in Puerto Rico.
Detection of KPC in Acinetobacter spp. in Puerto Rico.
Detection of KPC in Acinetobacter spp. in Puerto Rico.
Direct Ertapenem Disk Screening Method for Identification of KPC-Producing Klebsiella pneumoniae and Escherichia coli in Surveillance Swab Specimens
The study describes a direct ertapenem disk screening method for identifying KPC-producing K. pneumoniae and E. coli in surveillance swab specimens, demonstrating high sensitivity and specificity compared to traditional methods.
Emergence of KPC-producing Klebsiella pneumoniae in Italy.
The study reports the first two cases of KPC-2 producing K. pneumoniae in Italy, highlighting the global spread of KPC-mediated carbapenem resistance.
Outbreak of carbapenem-resistant Klebsiella pneumoniae in Puerto Rico associated with a novel carbapenemase variant.
Outbreak of carbapenem-resistant Klebsiella pneumoniae in Puerto Rico associated with a novel carbapenemase variant.
Outbreak of carbapenem-resistant Klebsiella pneumoniae in Puerto Rico associated with a novel carbapenemase variant.
Substrate selectivity and a novel role in inhibitor discrimination by residue 237 in the KPC-2 beta-lactamase.
Transfer of carbapenem-resistant plasmid from Klebsiella pneumoniae ST258 to Escherichia coli in patient.
The study reports the transfer of a carbapenem-resistant plasmid carrying blaKPC-3 from Klebsiella pneumoniae ST258 to Escherichia coli, resulting in resistance to carbapenems and cephalosporins.
Carbapenem-resistant Acinetobacter baumannii and Klebsiella pneumoniae across a hospital system: impact of post-acute care facilities on dissemination.
The study identifies blaOXA-23 and blaOXA-24/40 as the primary carbapenem resistance genes in Acinetobacter baumannii, and blaKPC-2 and blaKPC-3 in Klebsiella pneumoniae, highlighting the role of post-acute care facilities in the dissemination of these resistant strains.
Carbapenem-resistant Acinetobacter baumannii and Klebsiella pneumoniae across a hospital system: impact of post-acute care facilities on dissemination.
The study identifies blaOXA-23 and blaOXA-24/40 as the primary carbapenem resistance genes in Acinetobacter baumannii, and blaKPC-2 and blaKPC-3 in Klebsiella pneumoniae, highlighting the role of post-acute care facilities in the dissemination of these resistant strains.
Carbapenem-resistant Acinetobacter baumannii and Klebsiella pneumoniae across a hospital system: impact of post-acute care facilities on dissemination.
Evaluation of a DNA microarray, the check-points ESBL/KPC array, for rapid detection of TEM, SHV, and CTX-M extended-spectrum beta-lactamases and KPC carbapenemases.
The Check-Points ESBL/KPC array effectively detects TEM, SHV, CTX-M, and KPC beta-lactamase genes, offering rapid and accurate identification of extended-spectrum beta-lactamases and carbapenemases in clinical isolates.
Genetic factors associated with elevated carbapenem resistance in KPC-producing Klebsiella pneumoniae.
The study identifies blaKPC as a key gene contributing to carbapenem resistance in KPC-producing Klebsiella pneumoniae, with elevated copy numbers and upstream deletions enhancing resistance levels.
Elucidating the role of Trp105 in the KPC-2 beta-lactamase.
The study elucidates the role of Trp105 in the KPC-2 beta-lactamase, demonstrating that substitutions at this position affect substrate and inhibitor specificity, leading to altered resistance profiles against various β-lactams and β-lactam-beta-lactamase inhibitors.
Successful treatment of pan-resistant Klebsiella pneumoniae pneumonia and bacteraemia with a combination of high-dose tigecycline and colistin.
The study reports the successful treatment of pan-resistant Klebsiella pneumoniae bacteraemia using a combination of high-dose tigecycline and colistin. The isolate was found to produce the KPC-2 carbapenemase, which confers resistance to carbapenems.
Worldwide diversity of Klebsiella pneumoniae that produce beta-lactamase blaKPC-2 gene.
The study identifies various beta-lactamase genes including blaKPC-2, blaSHV-1, blaSHV-11, blaOKP-A/B, blaTEM-1, blaCTX-M-2, blaCTX-M-12, blaCTX-M-15, and blaOXA-9 in K. pneumoniae isolates from multiple countries, highlighting the diversity and global spread of these resistance genes.
Outbreak of colistin-resistant, carbapenem-resistant Klebsiella pneumoniae in metropolitan Detroit, Michigan.
The study identifies the presence of blaKPC genes in colistin-resistant, carbapenem-resistant Klebsiella pneumoniae isolates, indicating the mechanism of carbapenem resistance.
Multiplex real-time PCR assay for detection and classification of Klebsiella pneumoniae carbapenemase gene (blaKPC) variants.
The study describes a multiplex real-time PCR assay for the detection and classification of blaKPC variants, including blaKPC-2 and blaKPC-3, which are prevalent in the New York/New Jersey region.
Multiplex real-time PCR assay for detection and classification of Klebsiella pneumoniae carbapenemase gene (blaKPC) variants.
The study describes a multiplex real-time PCR assay for the detection and classification of blaKPC variants, including blaKPC-2 and blaKPC-3, which are prevalent in the New York/New Jersey region.
Novel genetic environment of the plasmid-mediated KPC-3 gene detected in Escherichia coli and Citrobacter freundii isolates from China.
The study identifies the plasmid-mediated KPC-3 gene in Escherichia coli and Citrobacter freundii isolates from China, highlighting its novel genetic environment and the impact of a 671-bp insertion on its expression.
Plasmid comparison and molecular analysis of Klebsiella pneumoniae harbouring bla(KPC) from New York City and Toronto.
Transfer of KPC-2 Carbapenemase from Klebsiella pneumoniae to Escherichia coli in a patient: first case in Europe.
The study describes the first case in Europe of KPC-2 carbapenemase transfer from Klebsiella pneumoniae to Escherichia coli in the same patient, demonstrating horizontal plasmid transfer and the emergence of carbapenem resistance in E. coli.
First report of Klebsiella oxytoca strain coproducing KPC-2 and IMP-8 carbapenemases.
The study reports the first case of a Klebsiella oxytoca strain coproducing plasmid-mediated KPC-2 and IMP-8 carbapenemases, highlighting their role in high-level resistance to imipenem and ertapenem.
Rapid Detection of blaKPC Carbapenemase Genes by Internally Controlled Real-Time PCR Assay Using Bactec Blood Culture Bottles
The study validated a real-time PCR assay for the rapid detection of blaKPC carbapenemase genes in Bactec blood culture bottles, demonstrating 100% sensitivity and specificity for detecting carbapenem-resistant bacteria.
Susceptibility of Klebsiella pneumoniae isolates from intra-abdominal infections and molecular characterization of ertapenem-resistant isolates.
The study identified KPC-2, KPC-3, KPC-11, OXA-48, SHV-12, and CTX-M-15 as the primary resistance mechanisms in ertapenem-resistant Klebsiella pneumoniae isolates.
Susceptibility of Klebsiella pneumoniae isolates from intra-abdominal infections and molecular characterization of ertapenem-resistant isolates.
The study identified KPC-2, KPC-3, KPC-11, OXA-48, SHV-12, and CTX-M-15 as the primary resistance mechanisms in ertapenem-resistant Klebsiella pneumoniae isolates.
Susceptibility of Klebsiella pneumoniae isolates from intra-abdominal infections and molecular characterization of ertapenem-resistant isolates.
The study identified KPC-2, KPC-3, KPC-11, OXA-48, SHV-12, and CTX-M-15 as the primary resistance mechanisms in ertapenem-resistant Klebsiella pneumoniae isolates.
Susceptibility of Klebsiella pneumoniae isolates from intra-abdominal infections and molecular characterization of ertapenem-resistant isolates.
Detection of clinically important beta-lactamases in commensal Escherichia coli of human and swine origin in western China.
The study identified various beta-lactamase genes, including bla CTX-M-1 group variants and bla SHV, as prevalent in commensal E. coli from humans and swine in western China. Additionally, bla CMY-2 was commonly found in both human and swine isolates, while bla KPC was detected in some human isolates.
Isolation of a Klebsiella pneumoniae isolate of sequence type 258 producing KPC-2 carbapenemase in Korea.
The study reports the isolation of a KPC-2-producing Klebsiella pneumoniae isolate of sequence type 258 in Korea, which exhibits resistance to multiple antibiotics including carbapenems.
Molecular dissection of an outbreak of carbapenem-resistant enterobacteriaceae reveals Intergenus KPC carbapenemase transmission through a promiscuous plasmid.
The study identifies the blaKPC gene as a key factor in carbapenem resistance among various Enterobacteriaceae species, highlighting the role of a promiscuous plasmid in the transmission of this resistance gene.
Molecular diversity in mechanisms of carbapenem resistance in paediatric Enterobacteriaceae.
The study identifies various carbapenem resistance mechanisms in pediatric Enterobacteriaceae, including plasmid-mediated beta-lactamases (CTX-M, CMY, KPC, IMP), chromosomal beta-lactamase (SME), and porin alterations (ompK35 and ompK36).
Emergence of New Delhi metallo-beta-lactamase (NDM-1) and Klebsiella pneumoniae carbapenemase (KPC-2) in South Africa.
The study reports the emergence of NDM-1 and KPC-2 carbapenemases in K. pneumoniae and E. cloacae in South Africa, highlighting their association with multidrug resistance and the need for improved detection strategies.
blaKPC and rmtB on a single plasmid in Enterobacter amnigenus and Klebsiella pneumoniae isolates from the same patient.
The study reports the identification of blaKPC-2 and rmtB genes on a single plasmid in Enterobacter amnigenus and Klebsiella pneumoniae isolates from the same patient, highlighting the coexistence of carbapenem and aminoglycoside resistance mechanisms.
Phenotypic Screening of Carbapenemases and Associated β-Lactamases in Carbapenem-Resistant Enterobacteriaceae.
The study identifies and characterizes various carbapenemases, including blaIMP, blaVIM, blaNDM-1, blaKPC, and blaOXA-48, in carbapenem-resistant Enterobacteriaceae through phenotypic testing with inhibitor-impregnated agar.
Clinical and Microbiologic Characteristics of Cephalosporin-Resistant Escherichia coli at Three Centers in the United States.
The study identified CTX-M-15 as the most common ESBL in cephalosporin-resistant E. coli, followed by CMY-2, SHV, TEM, and KPC beta-lactamases. These genes confer resistance to various beta-lactam antibiotics.
First description of KPC-2-producing Pseudomonas putida in Brazil.
The study reports the first case of a KPC-2-producing Pseudomonas putida isolate in Brazil, highlighting the presence of the blaKPC-2 gene and a novel rifampin resistance allele arr-6.
Mechanisms of resistance and clinical relevance of resistance to β-lactams, glycopeptides, and fluoroquinolones.
The paper discusses the mechanisms of resistance to β-lactams, glycopeptides, and fluoroquinolones, highlighting the role of beta-lactamases such as TEM, SHV, CTX-M, KPC, VIM, and NDM, glycopeptide resistance operons like vanA and vanB, and other resistance genes such as ermB, mecA, qnrA, and aac(6')-Ib.
Using nucleic acid microarrays to perform molecular epidemiology and detect novel beta-lactamases: a snapshot of extended-spectrum beta-lactamases throughout the world.
The study identifies two novel beta-lactamase variants, bla SHV-129 and bla KPC-11, in clinical isolates. These genes confer resistance to various β-lactam antibiotics.
Using nucleic acid microarrays to perform molecular epidemiology and detect novel β-lactamases: a snapshot of extended-spectrum β-lactamases throughout the world.
Using nucleic acid microarrays to perform molecular epidemiology and detect novel β-lactamases: a snapshot of extended-spectrum β-lactamases throughout the world.
Using nucleic acid microarrays to perform molecular epidemiology and detect novel β-lactamases: a snapshot of extended-spectrum β-lactamases throughout the world.
Emergence of Proteus mirabilis harboring blaKPC-2 and qnrD in a Chinese Hospital.
The study identifies the emergence of Proteus mirabilis isolates harboring blaKPC-2 and qnrD, highlighting the role of chromosomal mutations in quinolone resistance and plasmid-mediated carbapenem resistance.
Silent" Dissemination of Klebsiella pneumoniae Isolates Bearing K. pneumoniae Carbapenemase in a Long-term Care Facility for Children and Young Adults in Northeast Ohio.
The study identifies the blaKPC-3 gene as a carbapenem resistance determinant in Klebsiella pneumoniae isolates from a long-term care facility, highlighting its silent dissemination and the need for improved detection methods.
In vitro activity of MK-7655, a novel beta-lactamase inhibitor, in combination with imipenem against carbapenem-resistant Gram-negative bacteria.
MK-7655, a novel beta-lactamase inhibitor, significantly reduces imipenem MICs against carbapenem-resistant Gram-negative bacteria, demonstrating synergy in combination therapy.
Imported Klebsiella pneumoniae carbapenemase-producing K. pneumoniae clones in a Greek hospital: impact of infection control measures for restraining their dissemination.
The study identifies KPC-2 and VIM-1 carbapenemase-producing K. pneumoniae clones in a Greek hospital and highlights the effectiveness of infection control measures in reducing their dissemination.
Understanding the molecular determinants of substrate and inhibitor specificities in the Carbapenemase KPC-2: exploring the roles of Arg220 and Glu276.
The study identifies mutations at positions 220 and 276 in the KPC-2 β-lactamase that influence resistance to β-lactam antibiotics and β-lactamase inhibitors. The R220K mutation increases susceptibility to clavulanic acid, while R220M and E276A/D mutations enhance resistance.
Current epidemiology and growing resistance of gram-negative pathogens.
The paper highlights the emergence and spread of multidrug-resistant Gram-negative pathogens, focusing on extended-spectrum beta-lactamases (ESBLs) such as CTX-M-15 and CTX-M-14, carbapenemases like KPC-2, VIM-2, and IMP-6, and aminoglycoside-modifying enzymes such as aac(6')-Ib-cr and armA. It emphasizes the global dissemination of these resistance mechanisms and their impact on treatment options.
Activity of ceftaroline-avibactam tested against Gram-negative organism populations, including strains expressing one or more β-lactamases and methicillin-resistant Staphylococcus aureus carrying various staphylococcal cassette chromosome mec types.
Ceftaroline-avibactam showed potent activity against β-lactamase-producing Enterobacteriaceae, including those with multiple β-lactamases, and was effective against KPC-producing strains. It had limited activity against Acinetobacter spp. and P. aeruginosa.
Activity of ceftaroline-avibactam tested against Gram-negative organism populations, including strains expressing one or more β-lactamases and methicillin-resistant Staphylococcus aureus carrying various staphylococcal cassette chromosome mec types.
Ceftaroline-avibactam showed potent activity against β-lactamase-producing Enterobacteriaceae, including those with multiple β-lactamases, and was effective against KPC-producing strains. It had limited activity against Acinetobacter spp. and P. aeruginosa.
Activity of ceftaroline-avibactam tested against Gram-negative organism populations, including strains expressing one or more β-lactamases and methicillin-resistant Staphylococcus aureus carrying various staphylococcal cassette chromosome mec types.
Ceftaroline-avibactam showed potent activity against β-lactamase-producing Enterobacteriaceae, including those with multiple β-lactamases, and was effective against KPC-producing strains. It had limited activity against Acinetobacter spp. and P. aeruginosa.
Role of ISKpn7 and Deletions in blaKPC Gene Expression
The study identifies the role of ISKpn7 and deletions in the expression of the blaKPC gene, which encodes a carbapenemase. Different promoter sequences (P1, P2, and P3) upstream of blaKPC were found to affect gene expression levels, leading to variations in carbapenem MICs.
Outbreak of Klebsiella pneumoniae carbapenemase-2-producing K. pneumoniae sequence type 11 in Taiwan in 2011.
The study identifies the first outbreak of KPC-2-producing K. pneumoniae sequence type 11 in Taiwan, highlighting the spread of carbapenem resistance.
Klebsiella pneumoniae Carbapenemase-producing enterobacteria in hospital, Singapore.
The study identified a KPC-producing K. pneumoniae isolate carrying the blaKPC-2 gene, which confers resistance to carbapenems.
Exploring the role of a conserved class A residue in the Ω-Loop of KPC-2 beta-lactamase: a mechanism for ceftazidime hydrolysis.
The study identifies the role of the Arg-164 residue in the Ω-loop of KPC-2 beta-lactamase in ceftazidime hydrolysis and resistance. Substitutions at this residue, particularly R164S, enhance ceftazidime resistance through a covalent trapping mechanism.
Transmission dynamics of carbapenemase-producing Klebsiella pneumoniae and anticipated impact of infection control strategies in a surgical unit.
The study identifies blaKPC and blaVIM genes as responsible for carbapenem resistance in Klebsiella pneumoniae, highlighting their role in the transmission dynamics within a surgical unit.
Antibiotic resistance and substrate profiles of the class A carbapenemase KPC-6.
Antibiotic resistance and substrate profiles of the class A carbapenemase KPC-6.
Antibiotic resistance and substrate profiles of the class A carbapenemase KPC-6.
Antibiotic resistance and substrate profiles of the class A carbapenemase KPC-6.
KPC-9, a novel carbapenemase from clinical specimens in Israel.
The study identifies KPC-9, a novel carbapenemase variant differing from KPC-3 by a Val239Ala substitution, which confers reduced resistance to carbapenems but increased resistance to ceftazidime.
KPC-9, a novel carbapenemase from clinical specimens in Israel.
Rapid identification of pathogens from positive blood cultures by multiplex polymerase chain reaction using the FilmArray system.
The FilmArray BC panel accurately identifies pathogens and detects resistance genes such as mecA, vanA, and blaKPC from positive blood cultures.
Comparison of PCR/electron spray ionization-time-of-flight-mass spectrometry versus traditional clinical microbiology for active surveillance of organisms contaminating high-use surfaces in a burn intensive care unit, an orthopedic ward and healthcare workers.
The study compared PCR/ESI-TOF-MS with traditional clinical microbiology for detecting organisms and resistance genes in healthcare environments. It identified mecA, vanA, and KPC-3 resistance genes in various samples.
KPC-4 Is encoded within a truncated Tn4401 in an IncL/M plasmid, pNE1280, isolated from Enterobacter cloacae and Serratia marcescens.
The study identifies blaKPC-4 encoded within a truncated Tn4401 in an IncL/M plasmid, pNE1280, isolated from Enterobacter cloacae and Serratia marcescens, highlighting the transfer of carbapenem resistance between these species.
Complete nucleotide sequences of blaKPC-4- and blaKPC-5-harboring IncN and IncX plasmids from Klebsiella pneumoniae strains isolated in New Jersey.
The study reports the complete sequences of blaKPC-4- and blaKPC-5-harboring IncN and IncX plasmids from Klebsiella pneumoniae strains, highlighting the genetic diversity and mobility of these plasmids.
Complete nucleotide sequences of blaKPC-4- and blaKPC-5-harboring IncN and IncX plasmids from Klebsiella pneumoniae strains isolated in New Jersey.
The study reports the complete sequences of blaKPC-4- and blaKPC-5-harboring IncN and IncX plasmids from Klebsiella pneumoniae strains, highlighting the genetic diversity and mobility of these plasmids.
Epidemiology and resistance mechanisms to imipenem in Klebsiella pneumoniae: a multicenter study.
The study identified KPC-2 carbapenemase, blaCTX-M-9 ESBL, and blaDHA-1 cephalosporinase as the primary resistance mechanisms in imipenem-resistant Klebsiella pneumoniae isolates, leading to multidrug resistance.
Multidrug resistance genes, including blaKPC and blaCTX-M-2, among Klebsiella pneumoniae isolated in Recife, Brazil.
The study identified blaKPC and blaCTX-M-2 genes in multidrug-resistant Klebsiella pneumoniae isolates from Recife, Brazil, highlighting the emergence of carbapenem and cephalosporin resistance.
Epidemic of Klebsiella pneumoniae ST11 clone coproducing KPC-2 and 16S rRNA methylase RmtB in a Chinese University Hospital.
The study identifies the emergence of Klebsiella pneumoniae ST11 clones co-producing KPC-2 and the 16S rRNA methylase RmtB, which confer high-level resistance to aminoglycosides and carbapenems.
The modified Hodge test is a useful tool for ruling out Klebsiella pneumoniae carbapenemase.
The study evaluates the modified Hodge test for detecting Klebsiella pneumoniae carbapenemase (KPC) in Enterobacteriaceae isolates, showing that negative results effectively rule out KPC production.
Ertapenem disk performance to predict Klebsiella pneumoniae carbapenemase produced by Gram-negative bacilli isolated in a São Paulo city public hospital.
The study evaluated the performance of ertapenem disks to predict the presence of Klebsiella pneumoniae carbapenemase (KPC) in Gram-negative bacilli. It found that ertapenem disk resistance was not a reliable predictor of KPC production, as only 36% of resistant strains had the blaKPC gene.
Characterization of porin expression in Klebsiella pneumoniae Carbapenemase (KPC)-producing K. pneumoniae identifies isolates most susceptible to the combination of colistin and carbapenems.
The study characterizes the resistance mechanisms in KPC-producing K. pneumoniae isolates, highlighting the role of porin expression in determining susceptibility to colistin and carbapenem combinations.
"Stormy waters ahead": global emergence of carbapenemases.
The paper discusses the global emergence of carbapenemases, including KPC, NDM, and OXA-48, which confer resistance to carbapenems and other beta-lactam antibiotics. These enzymes are critical in the spread of multidrug-resistant Gram-negative bacteria.
A long-term low-frequency hospital outbreak of KPC-producing Klebsiella pneumoniae involving Intergenus plasmid diffusion and a persisting environmental reservoir.
The study identifies blaKPC-2 as a gene responsible for carbapenem resistance in KPC-producing Klebsiella pneumoniae and Enterobacter asburiae. The gene was found on a 97 kb IncFII plasmid and was transferred between different bacterial species and strains.
The first report of infection with Klebsiella pneumoniae carrying the bla(kpc) gene in State of Mato Grosso do Sul, Brazil.
The study reports the first case of infection by Klebsiella pneumoniae carrying the bla(kpc) gene in the State of Mato Grosso do Sul, Brazil.
First description of KPC-2-producing Klebsiella oxytoca in Brazil.
The study reports the first case of a KPC-2-producing K. oxytoca isolate in Brazil, which carries a 65-kb IncW-type plasmid harboring blaKPC and Tn4401b. Additionally, a novel rifampin resistance allele arr-8 was identified.
Septic shock caused by Klebsiella pneumoniae carbapenemase-producing Enterobacter gergoviae in a neutropenic patient with leukemia.
The study reports the first case of septic shock caused by KPC-producing Enterobacter gergoviae in a neutropenic patient with leukemia, highlighting the emergence of KPCs in non-Klebsiella species and the challenges in treating such infections.
Virulence of Klebsiella pneumoniae isolates harboring bla KPC-2 carbapenemase gene in a Caenorhabditis elegans model.
The study characterizes the bla KPC-2 gene as a carbapenemase gene that confers resistance to carbapenems in Klebsiella pneumoniae isolates.
Complete nucleotide sequence of a blaKPC-harboring IncI2 plasmid and its dissemination in New Jersey and New York hospitals.
The study reports the complete nucleotide sequence of a blaKPC-3-harboring IncI2 plasmid, pBK15692, and its prevalence in K. pneumoniae ST258 strains in New Jersey and New York hospitals.
Kinetics of avibactam inhibition against Class A, C, and D beta-lactamases.
Avibactam effectively inhibits Class A, C, and D beta-lactamases, with varying efficiencies. It shows potent activity against CTX-M-15 and KPC-2, moderate activity against AmpC enzymes, and lower activity against OXA-10 and OXA-48.
Tigecycline treatment of infection caused by KPC-producing Escherichia coli in a pediatric patient.
The study identified blaKPC-2 and blaCTX-M-24 genes in KPC-producing E. coli isolates, which conferred resistance to carbapenems and beta-lactams, respectively.
Rapid and Direct Real-Time Detection of blaKPC and blaNDM from Surveillance Samples
The study describes a duplex real-time PCR assay for the rapid and direct detection of blaKPC and blaNDM genes in surveillance samples, demonstrating high sensitivity and specificity for identifying carbapenemase-producing Enterobacteriaceae.
A ten years (2000-2009) surveillance of resistant Enterobacteriaceae in Zhejiang Province, China.
The study identified the prevalence of blaKPC-2 gene among carbapenem-resistant Enterobacteriaceae in Zhejiang Province, China, highlighting the significant increase in carbapenem resistance.
Detection of Colonization by Carbapenemase-Producing Gram-Negative Bacilli in Patients by Use of the Xpert MDRO Assay
The study evaluated the Xpert MDRO assay for detecting blaKPC, blaNDM, and blaVIM carbapenem resistance genes in clinical samples, demonstrating high sensitivity and specificity for identifying carbapenemase-producing organisms.
Promoter deletions of Klebsiella pneumoniae carbapenemase (KPC)-encoding genes (blaKPC -2) and efflux pump (AcrAB) on β-lactam susceptibility in KPC-producing Enterobacteriaceae.
The study characterizes the impact of promoter deletions in blaKPC-2 genes and the efflux pump AcrAB on β-lactam susceptibility in KPC-producing Enterobacteriaceae. It shows that promoter deletions in blaKPC-2 genes correlate with variations in β-lactam MICs, and that the efflux pump AcrAB contributes to the full range of resistance.
Promoter deletions of Klebsiella pneumoniae carbapenemase (KPC)-encoding genes (blaKPC -2) and efflux pump (AcrAB) on β-lactam susceptibility in KPC-producing Enterobacteriaceae.
The study characterizes the impact of promoter deletions in blaKPC-2 genes and the efflux pump AcrAB on β-lactam susceptibility in KPC-producing Enterobacteriaceae. It shows that promoter deletions in blaKPC-2 genes correlate with variations in β-lactam MICs, and that the efflux pump AcrAB contributes to the full range of resistance.
Promoter deletions of Klebsiella pneumoniae carbapenemase (KPC)-encoding genes (blaKPC -2) and efflux pump (AcrAB) on β-lactam susceptibility in KPC-producing Enterobacteriaceae.
The study characterizes the impact of promoter deletions in blaKPC-2 genes and the efflux pump AcrAB on β-lactam susceptibility in KPC-producing Enterobacteriaceae. It shows that promoter deletions in blaKPC-2 genes correlate with variations in β-lactam MICs, and that the efflux pump AcrAB contributes to the full range of resistance.
Can inhibitor-resistant substitutions in the Mycobacterium tuberculosis β-Lactamase BlaC lead to clavulanate resistance?: a biochemical rationale for the use of β-lactam–β-lactamase inhibitor combinations.
Substitutions in the Mycobacterium tuberculosis β-lactamase BlaC, particularly R220A, R220S, A244R, S130G, T237A, and T237S, were found to reduce the effectiveness of clavulanate, although the wild-type BlaC remains susceptible to ampicillin-clavulanate.
Single or in combination antimicrobial resistance mechanisms of Klebsiella pneumoniae contribute to varied susceptibility to different carbapenems.
The study identified that blaCTX-M-15, blaSHV-12, blaDHA-1, ampR, blaKPC-2, and blaNDM-1 are responsible for carbapenem resistance in Klebsiella pneumoniae. The loss of porins OmpK35 and OmpK36 combined with these genes contributed to resistance against various carbapenems.
A Review of Ten Years of the Study for Monitoring Antimicrobial Resistance Trends (SMART) from 2002 to 2011.
The SMART study analyzed antimicrobial resistance trends in Gram-negative bacilli, identifying new β-lactamase variants (blaSHV-129, blaKPC-11) and highlighting the prevalence of carbapenem resistance mechanisms such as NDM-1.
Emergence of Escherichia coli sequence type 131 isolates producing KPC-2 carbapenemase in China.
The study reports the emergence of KPC-2-producing Escherichia coli sequence type 131 isolates in China, highlighting the spread of carbapenem resistance through the blaKPC-2 gene.
Updated molecular epidemiology of carbapenem-non-susceptible Escherichia coli in Taiwan: first identification of KPC-2 or NDM-1-producing E. coli in Taiwan.
The study identifies the first occurrence of KPC-2 and NDM-1 producing E. coli in Taiwan, highlighting the emergence of these carbapenemases as significant contributors to carbapenem resistance. The main resistance mechanism involves plasmidic AmpC β-lactamases (CMY-2 and DHA-1) in combination with loss of outer membrane porins OmpC and OmpF.
Multi-centre evaluation of real-time multiplex PCR for detection of carbapenemase genes OXA-48, VIM, IMP, NDM and KPC.
The study presents a real-time multiplex PCR assay for detecting the most prevalent carbapenemase genes, including blaOXA-48, blaVIM, blaIMP, blaNDM, and blaKPC, demonstrating high sensitivity and specificity.
Comparative analysis of the complete genome of KPC-2-producing Klebsiella pneumoniae Kp13 reveals remarkable genome plasticity and a wide repertoire of virulence and resistance mechanisms.
The study identifies multiple beta-lactamase genes (blaSHV-12, blaOXA-9, blaTEM-1, blaCTX-M-2, blaSHV-110, blaKPC-2) and the rmtG gene, which confer resistance to beta-lactams and aminoglycosides, respectively. Additionally, mutations in gyrA and parC contribute to fluoroquinolone resistance in Kp13.
First documented outbreak of KPC-2-producing Klebsiella pneumoniae in Switzerland: infection control measures and clinical management.
The study documents the first outbreak of KPC-2-producing Klebsiella pneumoniae in Switzerland, highlighting the role of the blaKPC-2 gene in carbapenem resistance and the effectiveness of infection control measures.
Molecular survey of the dissemination of two blaKPC-harboring IncFIA plasmids in New Jersey and New York hospitals.
The study identified two blaKPC-3-harboring IncFIA plasmids, pBK30661 and pBK30683, which contribute to carbapenem resistance in Klebsiella pneumoniae and other Enterobacteriaceae.
blaKPC gene Detection in Clinical Isolates of Carbapenem Resistant Enterobacteriaceae in a Tertiary Care Hospital.
The study identified the blaKPC gene as a significant cause of carbapenem resistance in Enterobacteriaceae, with 67.4% of carbapenem-resistant isolates testing positive for the gene.
Modified CLSI extended-spectrum β-lactamase (ESBL) confirmatory test for phenotypic detection of ESBLs among Enterobacteriaceae producing various β-lactamases.
The study evaluated a modified CLSI ESBL confirmatory test for the phenotypic detection of extended-spectrum β-lactamases (ESBLs) among Enterobacteriaceae producing various β-lactamases, including KPC, VIM, NDM, and OXA-48. The modified test significantly improved the sensitivity and specificity of ESBL detection compared to the standard CLSI test.
Improving the Efficiency of the Modified Hodge Test in KPC-Producing Klebsiella pneumoniae Isolates by Incorporating an EDTA Disk
The study demonstrates that the MHT-EDTA test improves the accuracy of detecting KPC-producing K. pneumoniae isolates, with 100% sensitivity and specificity. The KPC-2 carbapenemase was the most prevalent beta-lactamase in the isolates.
Travel-related carbapenemase-producing Gram-negative bacteria in Alberta, Canada: the first 3 years.
The study identifies carbapenemase-producing Gram-negative bacteria, including NDM-1, KPC-2, KPC-3, and OXA-23, in patients with a history of travel to India and other countries.
Travel-related carbapenemase-producing Gram-negative bacteria in Alberta, Canada: the first 3 years.
The study identifies carbapenemase-producing Gram-negative bacteria, including NDM-1, KPC-2, KPC-3, and OXA-23, in patients with a history of travel to India and other countries.
Comparative genomic analysis of KPC-encoding pKpQIL-like plasmids and their distribution in New Jersey and New York Hospitals.
The study identifies and characterizes pKpQIL-like plasmids carrying blaKPC-2 and blaKPC-3 genes, which confer resistance to carbapenems in various bacterial species, including Klebsiella pneumoniae, Escherichia coli, and Enterobacter aerogenes.
Comparative genomic analysis of KPC-encoding pKpQIL-like plasmids and their distribution in New Jersey and New York Hospitals.
The study identifies and characterizes pKpQIL-like plasmids carrying blaKPC-2 and blaKPC-3 genes, which confer resistance to carbapenems in various bacterial species, including Klebsiella pneumoniae, Escherichia coli, and Enterobacter aerogenes.
First report of Klebsiella pneumonia carbapenemase-producing Pseudomonas aeruginosa isolated from burn patients in Iran: phenotypic and genotypic methods.
The study reports the first identification of KPC-producing P. aeruginosa in Iran, highlighting the need for PCR as a reliable method for detecting KPC producers due to limitations in phenotypic tests.
Plasmid transferability of KPC into a virulent K2 serotype Klebsiella pneumoniae.
The study demonstrates the successful conjugation of KPC-2 and KPC-3 plasmids into a virulent K2 serotype Klebsiella pneumoniae, resulting in resistance to all beta-lactams.
Evaluation of an automated rapid diagnostic assay for detection of Gram-negative bacteria and their drug-resistance genes in positive blood cultures.
The study evaluated the Verigene BC-GN assay for rapid detection of Gram-negative bacteria and their drug-resistance genes in positive blood cultures. It successfully detected 9 bacterial species and 9 drug resistance genes, including bla CTX-M, bla IMP, bla KPC, bla NDM, bla OXA-23, bla OXA-24/40, bla OXA-48, bla OXA-58, and bla VIM.
Phenotypic and molecular characterization of multidrug resistant Klebsiella pneumoniae isolated from a university teaching hospital, China.
The study identified multiple AMR genes in multidrug-resistant Klebsiella pneumoniae isolates, including bla TEM, bla CTX-M, bla DHA, and bla KPC, which contribute to resistance against various antibiotics.
Use of imipenem to detect KPC, NDM, OXA, IMP, and VIM carbapenemase activity from gram-negative rods in 75 minutes using liquid chromatography-tandem mass spectrometry.
The study developed an LC-MS/MS assay to detect carbapenemase activity from gram-negative rods, demonstrating that imipenem showed high sensitivity and specificity for detecting KPC, NDM, OXA, IMP, and VIM carbapenemases.
Complex prosthetic joint infections due to carbapenemase-producing Klebsiella pneumoniae: a unique challenge in the era of untreatable infections.
The study highlights the challenges of treating complex prosthetic joint infections caused by carbapenemase-producing Klebsiella pneumoniae (CRKP), emphasizing the difficulty in eradicating these infections and the emergence of resistance to last-line antibiotics such as colistin and amikacin.
High dose tigecycline in critically ill patients with severe infections due to multidrug-resistant bacteria.
The study identifies bla OXA-58, bla OXA-23, and bla KPC-3 genes as responsible for carbapenem resistance in Acinetobacter baumannii and Klebsiella pneumoniae isolates from critically ill patients with multidrug-resistant infections.
First isolate of KPC-2-producing Klebsiella pneumonaie sequence type 23 from the Americas.
The study reports the first isolation of a KPC-2-producing Klebsiella pneumoniae sequence type 23 in the Americas, highlighting the emergence of this multidrug-resistant clone.
Microbiological features of KPC-producing Enterobacter isolates identified in a U.S. hospital system.
The study identifies blaKPC-2 and blaKPC-3 as the primary carbapenem resistance genes in KPC-producing Enterobacter isolates, along with various ESBL genes such as blaSHV-5, blaSHV-12, blaSHV-154, blaCTX-M-15, and blaTEM-1. Additionally, plasmid-mediated fluoroquinolone resistance genes qnrA and qnrB were detected.
Microbiological features of KPC-producing Enterobacter isolates identified in a U.S. hospital system.
The study identifies blaKPC-2 and blaKPC-3 as the primary carbapenem resistance genes in KPC-producing Enterobacter isolates, along with various ESBL genes such as blaSHV-5, blaSHV-12, blaSHV-154, blaCTX-M-15, and blaTEM-1. Additionally, plasmid-mediated fluoroquinolone resistance genes qnrA and qnrB were detected.
Rapid identification of carbapenemase genes in gram-negative bacteria with an oligonucleotide microarray-based assay.
The study presents a microarray-based assay for the rapid identification of carbapenemase genes in Gram-negative bacteria, including bla KPC, bla VIM, bla NDM, bla GIM, bla OXA-23, bla OXA-48-group, bla OXA-51, bla OXA-58, and various beta-lactamase genes such as bla OXA-1, bla OXA-2, bla OXA-7, bla OXA-9, bla OXA-10, bla CTX-M1, and bla CTX-M15.
Rapid identification of carbapenemase genes in gram-negative bacteria with an oligonucleotide microarray-based assay.
The study presents a microarray-based assay for the rapid identification of carbapenemase genes in Gram-negative bacteria, including bla KPC, bla VIM, bla NDM, bla GIM, bla OXA-23, bla OXA-48-group, bla OXA-51, bla OXA-58, and various beta-lactamase genes such as bla OXA-1, bla OXA-2, bla OXA-7, bla OXA-9, bla OXA-10, bla CTX-M1, and bla CTX-M15.
Rapid identification of carbapenemase genes in gram-negative bacteria with an oligonucleotide microarray-based assay.
The study presents a microarray-based assay for the rapid identification of carbapenemase genes in Gram-negative bacteria, including bla KPC, bla VIM, bla NDM, bla GIM, bla OXA-23, bla OXA-48-group, bla OXA-51, bla OXA-58, and various beta-lactamase genes such as bla OXA-1, bla OXA-2, bla OXA-7, bla OXA-9, bla OXA-10, bla CTX-M1, and bla CTX-M15.
Spread of carbapenemase-producing enterobacteria in a southwest hospital in China.
The study identified IMP-4 and KPC-2 as carbapenemase genes responsible for carbapenem resistance in Enterobacteriaceae isolates from a hospital in China.
Carbapenem-resistant Enterobacter cloacae isolates producing KPC-3, North Dakota, USA.
The study identifies KPC-3-producing carbapenem-resistant Enterobacter cloacae isolates in North Dakota, highlighting the clonal nature of the outbreak and the role of long-term acute-care facilities in dissemination.
Phenotypic and molecular characteristics of carbapenem-resistant Enterobacteriaceae in a health care system in Los Angeles, California, from 2011 to 2013.
The study identified bla KPC, bla SME, and bla NDM-1 as the primary carbapenemase-encoding genes in carbapenem-resistant Enterobacteriaceae (CRE) isolates, with bla KPC being the most common. These genes conferred resistance to carbapenems such as meropenem and imipenem.
Genomic Analysis of Carbapenemase-Producing Enterobacteriaceae in a Hospital Setting
The study identifies the blaKPC gene as a key determinant of carbapenem resistance in various Enterobacteriaceae species, highlighting the role of plasmid-mediated resistance and horizontal gene transfer in hospital settings.
ISEcp1-mediated transposition of blaKPC into the chromosome of a clinical isolate of Acinetobacter baumannii from Puerto Rico.
The study reports the chromosomal integration of the blaKPC-3 gene in Acinetobacter baumannii through an IS Ecp1-mediated transposition event, highlighting the genetic mobility of carbapenem resistance mechanisms.
Evaluation of inhibitory action of novel non β-lactam inhibitor against Klebsiella pneumoniae carbapenemase (KPC-2).
The study identified ZINC01807204 as a novel non-beta-lactam inhibitor effective against KPC-2, reducing the MIC of carbapenems significantly.
Rapid Diagnostic Approaches for Antimicrobial Resistance
The paper discusses various rapid antimicrobial resistance testing methods, including molecular techniques like qPCR, DNA microarrays, Luminex xMAP, and next-generation sequencing (NGS), highlighting their roles in detecting resistance genes such as blaCTX-M, blaTEM, blaSHV, blaKPC, blaNDM, blaOXA-48, blaVIM, blaIMP, mecA, mecC, vanA, vanB, aacA-aphD, tetK, tetM, ermA, ermC, vatA, vatB, and vatC in different bacterial species.
Evaluation of carbapenemase screening and confirmation tests with Enterobacteriaceae and development of a practical diagnostic algorithm.
The study identified several carbapenemase genes, including blaKPC, blaVIM, blaNDM, and blaOXA-48, which confer resistance to carbapenems in Enterobacteriaceae. These genes were experimentally validated through genetic detection and phenotypic testing.
Emergence of KPC-producing Klebsiella pneumoniae in Uruguay: infection control and molecular characterization.
The study reports the first outbreak of KPC-producing Klebsiella pneumoniae in Uruguay, highlighting the emergence of multidrug-resistant strains with resistance to carbapenems, colistin, and tigecycline. The bla KPC-2 gene was identified as the primary resistance determinant.
Emergence of Klebsiella pneumoniae clinical isolates producing KPC-2 carbapenemase in Cuba.
The study reports the first detection of KPC-2-producing Klebsiella pneumoniae in Cuba, highlighting the emergence of carbapenem resistance in clinical isolates.
Phenotypic and enzymatic comparative analysis of the KPC variants, KPC-2 and its recently discovered variant KPC-15.
The study characterized the KPC-15 variant, a carbapenemase with higher catalytic efficiency compared to KPC-2 for various beta-lactam antibiotics. Both KPC-15 and KPC-2 conferred resistance to multiple beta-lactam antibiotics, but KPC-15 showed improved efficiency in hydrolyzing certain substrates.
Phenotypic and enzymatic comparative analysis of the KPC variants, KPC-2 and its recently discovered variant KPC-15.
The study characterized the KPC-15 variant, a carbapenemase with higher catalytic efficiency compared to KPC-2 for various beta-lactam antibiotics. Both KPC-15 and KPC-2 conferred resistance to multiple beta-lactam antibiotics, but KPC-15 showed improved efficiency in hydrolyzing certain substrates.
Phenotypic and enzymatic comparative analysis of the KPC variants, KPC-2 and its recently discovered variant KPC-15.
Phenotypic and enzymatic comparative analysis of the KPC variants, KPC-2 and its recently discovered variant KPC-15.
Phenotypic and enzymatic comparative analysis of the KPC variants, KPC-2 and its recently discovered variant KPC-15.
Phenotypic and enzymatic comparative analysis of the KPC variants, KPC-2 and its recently discovered variant KPC-15.
Phenotypic and enzymatic comparative analysis of the KPC variants, KPC-2 and its recently discovered variant KPC-15.
Phenotypic and enzymatic comparative analysis of the KPC variants, KPC-2 and its recently discovered variant KPC-15.
First report of a clinical, multidrug-resistant Enterobacteriaceae isolate coharboring fosfomycin resistance gene fosA3 and carbapenemase gene blaKPC-2 on the same transposon, Tn1721.
The study reports the first occurrence of the fosfomycin resistance gene fosA3 and the carbapenemase gene blaKPC-2 coexisting on the same transposon, Tn1721, within a conjugative IncP plasmid in clinical Enterobacteriaceae isolates.
First Description of KPC-2-Producing Klebsiella oxytoca Isolated from a Pediatric Patient with Nosocomial Pneumonia in Venezuela.
The study describes the first nosocomial KPC-2-producing Klebsiella oxytoca isolate in Venezuela, which was resistant to carbapenems, cephalosporins, and aminoglycosides but susceptible to ciprofloxacin, colistin, and tigecycline. The isolate carried a 21-kb conjugative plasmid with blaKPC-2, blaCTX-M-8, and blaTEM-15 genes.
Emergence of novel bla(KPC-13) among carbapenem-resistant Enterobacteriaceae in Thailand.
Emergence of novel bla(KPC-13) among carbapenem-resistant Enterobacteriaceae in Thailand.
Emergence of novel bla(KPC-13) among carbapenem-resistant Enterobacteriaceae in Thailand.
Unusual association of NDM-1 with KPC-2 and armA among Brazilian Enterobacteriaceae isolates.
The study identifies the coexistence of blaNDM-1, blaKPC-2, and armA in Brazilian Enterobacteriaceae isolates, highlighting the complex resistance mechanisms in these pathogens.
Molecular characterization of Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae in Ontario, Canada, 2008-2011.
The study identifies bla KPC-2 and bla KPC-3 as the primary genes responsible for carbapenem resistance in KPC-producing Enterobacteriaceae in Ontario, Canada, from 2008 to 2011.
Molecular characterization of Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae in Ontario, Canada, 2008-2011.
The study identifies bla KPC-2 and bla KPC-3 as the primary genes responsible for carbapenem resistance in KPC-producing Enterobacteriaceae in Ontario, Canada, from 2008 to 2011.
Four carbapenem-resistant gram-negative species carrying distinct carbapenemases in a single patient.
The study reports the identification of four carbapenem-resistant Gram-negative species, each carrying distinct carbapenemases: NDM-1 in E. coli, KPC-2 in K. pneumoniae, IMP-1 in E. aerogenes, and OXA-23 in A. baumannii. Additionally, the 16S rRNA methyltransferase genes armA and rmtB were identified, contributing to aminoglycoside resistance.
Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae at a single institution: insights into endemicity from whole-genome sequencing.
The study identifies two novel conjugative bla KPC plasmids, pKPC_UVA01 and pKPC_UVA02, which contributed to the spread of carbapenem resistance among K. pneumoniae strains in a U.S. hospital. It also highlights the emergence of a new KPC- Kp lineage, ST941, which acquired these plasmids and spread locally.
Emergence of KPC new variants (KPC-16 and KPC-17) and ongoing outbreak in southern Taiwan.
The study identifies two novel KPC variants, KPC-16 and KPC-17, which confer resistance to carbapenems. These variants were found in Klebsiella pneumoniae isolates during an outbreak in southern Taiwan.
Emergence of KPC new variants (KPC-16 and KPC-17) and ongoing outbreak in southern Taiwan.
The study identifies two novel KPC variants, KPC-16 and KPC-17, which confer resistance to carbapenems. These variants were found in Klebsiella pneumoniae isolates during an outbreak in southern Taiwan.
Emergence of KPC new variants (KPC-16 and KPC-17) and ongoing outbreak in southern Taiwan.
Emergence of KPC new variants (KPC-16 and KPC-17) and ongoing outbreak in southern Taiwan.
Emergence of KPC new variants (KPC-16 and KPC-17) and ongoing outbreak in southern Taiwan.
Variants of beta-lactamase KPC-2 that are resistant to inhibition by avibactam.
The study identifies mutations in the KPC-2 beta-lactamase (S130G, R220M, and K234R) that confer resistance to avibactam, a beta-lactamase inhibitor. These variants show decreased susceptibility to ampicillin-avibactam, with significant reductions in avibactam's inhibitory effects.
Activities of ceftazidime, ceftaroline, and aztreonam alone and combined with avibactam against isogenic Escherichia coli strains expressing selected single beta-lactamases.
The study characterizes the activity of ceftazidime, ceftaroline, and aztreonam in combination with avibactam against isogenic E. coli strains expressing various beta-lactamase genes, demonstrating that avibactam effectively restores the activity of these β-lactams against strains producing class A, C, and D beta-lactamases.
Activities of ceftazidime, ceftaroline, and aztreonam alone and combined with avibactam against isogenic Escherichia coli strains expressing selected single beta-lactamases.
The study characterizes the activity of ceftazidime, ceftaroline, and aztreonam in combination with avibactam against isogenic E. coli strains expressing various beta-lactamase genes, demonstrating that avibactam effectively restores the activity of these β-lactams against strains producing class A, C, and D beta-lactamases.
Carbapenem-nonsusceptible Enterobacteriaceae in Taiwan.
The study identified several carbapenemase genes, including blaKPC-2, blaIMP-8, blaNDM-1, and blaVIM-1, along with extended-spectrum beta-lactamase genes such as CTX-M, SHV, and ampC beta-lactamase genes like DHA and CMY, which contribute to carbapenem resistance in Enterobacteriaceae isolates in Taiwan.
Rapid Induction of High-Level Carbapenem Resistance in Heteroresistant KPC-Producing Klebsiella pneumoniae
The study identifies the role of the blaKPC gene in conferring carbapenem resistance in KPC-producing Klebsiella pneumoniae, highlighting the importance of this gene in the rapid induction of high-level resistance.
Ceftazidime-avibactam activity tested against Enterobacteriaceae isolates from U.S. hospitals (2011 to 2013) and characterization of β-lactamase-producing strains.
The study evaluated the activity of ceftazidime-avibactam against Enterobacteriaceae isolates and identified blaKPC-2 and blaVIM-4 as resistance genes in K. pneumoniae strains.
Carbapenem non-susceptible enterobacteriaceae in Quebec, Canada: results of a laboratory surveillance program (2010-2012).
The study identified various carbapenemase genes, including blaKPC, blaSME, blaOXA-48, blaNDM, and blaNMC, in carbapenem-non-susceptible Enterobacteriaceae isolates in Quebec, Canada. These genes were associated with resistance to carbapenems and other antibiotics.
Activity of ceftazidime/avibactam against isogenic strains of Escherichia coli containing KPC and SHV beta-lactamases with single amino acid substitutions in the Ω-loop.
The study identified that certain KPC-2 variants with single amino acid substitutions in the Ω-loop exhibited resistance to ceftazidime/avibactam, highlighting the potential for these mutations to reduce the efficacy of this antibiotic combination.
Identification of Gram-Negative Bacteria and Genetic Resistance Determinants from Positive Blood Culture Broths by Use of the Verigene Gram-Negative Blood Culture Multiplex Microarray-Based Molecular Assay.
The Verigene Gram-Negative Blood Culture (BC-GN) assay effectively identifies Gram-negative bacteria and detects genetic resistance determinants such as bla CTX-M, bla KPC, bla NDM, bla OXA, bla VIM, and bla IMP in positive blood culture broths.
Activity of Meropenem Combined with RPX7009, a Novel β-Lactamase Inhibitor, against Gram-Negative Clinical Isolates in New York City.
The study demonstrates that the combination of meropenem and RPX7009 effectively inhibits the growth of KPC-producing Enterobacteriaceae, including multidrug-resistant strains, highlighting the potential of RPX7009 as a β-lactamase inhibitor to restore meropenem activity against carbapenem-resistant organisms.
An outbreak of colistin-resistant Klebsiella pneumoniae carbapenemase-producing Klebsiella pneumoniae in the Netherlands (July to December 2013), with inter-institutional spread.
The study describes an outbreak of colistin-resistant KPC-producing K. pneumoniae ST258 in the Netherlands, highlighting the presence of multiple resistance genes including blaKPC-2, blaSHV-12, and various other AMR genes.
Point-of-care multiplex PCR promises short turnaround times for microbial testing in hospital-acquired pneumonia--an observational pilot study in critical ill patients.
The study evaluated the performance of a point-of-care multiplex PCR system for detecting pathogens and antibiotic resistance markers in hospital-acquired pneumonia. The system showed significantly shorter turnaround times compared to conventional microbiological diagnostics, although its performance was limited by frequent test failures and non-concordant results.
pKBuS13, a KPC-2-encoding plasmid from Klebsiella pneumoniae sequence type 833, carrying Tn4401b inserted into an Xer site-specific recombination locus.
The study identifies pKBuS13, a plasmid carrying the blaKPC-2 gene, which confers resistance to carbapenems in Klebsiella pneumoniae ST833. The plasmid's instability and insertion of Tn4401b into an Xer recombination locus are noted.
Evaluation of Remel Spectra CRE Agar for Detection of Carbapenem-Resistant Bacteria from Rectal Swabs Obtained from Residents of a Long-Term-Care Facility.
The study evaluated the effectiveness of Remel Spectra CRE agar for detecting carbapenem-resistant Enterobacteriaceae (CRE) and found that it detected KPC-type CRE in 97.2% of cases, demonstrating its utility as a fast and reliable method for CRE detection.
In vitro selection of ceftazidime-avibactam resistance in Enterobacteriaceae with KPC-3 carbapenemase.
In vitro selection of ceftazidime-avibactam resistance in Enterobacteriaceae with KPC-3 carbapenemase.
In vitro selection of ceftazidime-avibactam resistance in Enterobacteriaceae with KPC-3 carbapenemase.
In vitro selection of ceftazidime-avibactam resistance in Enterobacteriaceae with KPC-3 carbapenemase.
Emergence of KPC-2-producing Escherichia coli isolates in an urban river in Harbin, China.
The study identifies KPC-2-producing Escherichia coli isolates in an urban river in Harbin, China, highlighting the presence of carbapenemase genes in environmental samples.
Effects of Klebsiella pneumoniae carbapenemase subtypes, extended-spectrum beta-lactamases, and porin mutations on the in vitro activity of ceftazidime-avibactam against carbapenem-resistant K. pneumoniae.
The study shows that ceftazidime-avibactam is effective against carbapenem-resistant K. pneumoniae strains, but the effectiveness varies depending on the presence of specific resistance mechanisms such as KPC subtypes and porin mutations.
Effects of Klebsiella pneumoniae carbapenemase subtypes, extended-spectrum beta-lactamases, and porin mutations on the in vitro activity of ceftazidime-avibactam against carbapenem-resistant K. pneumoniae.
The study shows that ceftazidime-avibactam is effective against carbapenem-resistant K. pneumoniae strains, but the effectiveness varies depending on the presence of specific resistance mechanisms such as KPC subtypes and porin mutations.
Community-Acquired Pyelonephritis in Pregnancy Caused by KPC-Producing Klebsiella pneumoniae.
The study identifies a KPC-2 producing Klebsiella pneumoniae strain responsible for community-acquired pyelonephritis in a pregnant woman, highlighting the challenges in treating infections caused by carbapenem-resistant Enterobacteriaceae (CRE).
First Report of Ceftazidime-Avibactam Resistance in a KPC-3-Expressing Klebsiella pneumoniae Isolate.
This study reports the first case of ceftazidime-avibactam resistance in a KPC-3-expressing Klebsiella pneumoniae isolate, highlighting the potential for resistance mechanisms to emerge against novel antimicrobial combinations.
Genomic Analysis of the Emergence and Rapid Global Dissemination of the Clonal Group 258 Klebsiella pneumoniae Pandemic.
The study identifies the blaKPC gene as a key factor in the emergence and global spread of multidrug-resistant Klebsiella pneumoniae ST258, highlighting its role in carbapenem resistance.
Genomically Informed Surveillance for Carbapenem-Resistant Enterobacteriaceae in a Health Care System.
The study identified KPC-2, KPC-3, KPC-4, and OXA-48 carbapenemase genes in carbapenem-resistant Enterobacteriaceae isolates, which were carried on various transposons and plasmids, facilitating the spread of resistance.
Genomically Informed Surveillance for Carbapenem-Resistant Enterobacteriaceae in a Health Care System.
The study identified KPC-2, KPC-3, KPC-4, and OXA-48 carbapenemase genes in carbapenem-resistant Enterobacteriaceae isolates, which were carried on various transposons and plasmids, facilitating the spread of resistance.
Genomically Informed Surveillance for Carbapenem-Resistant Enterobacteriaceae in a Health Care System.
The study identified KPC-2, KPC-3, KPC-4, and OXA-48 carbapenemase genes in carbapenem-resistant Enterobacteriaceae isolates, which were carried on various transposons and plasmids, facilitating the spread of resistance.
KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil.
The study identifies KPC-2-producing Klebsiella pneumoniae isolates in a Brazilian hospital, demonstrating the presence of the blaKPC-2 gene responsible for carbapenem resistance.
Clinical Performance of Check-Direct CPE, a Multiplex PCR for Direct Detection of bla(KPC), bla(NDM) and/or bla(VIM), and bla(OXA)-48 from Perirectal Swabs.
The study evaluated the clinical performance of Check-Direct CPE for the detection of blaKPC, blaNDM, blaVIM, and blaOXA-48 genes directly from perirectal swabs, demonstrating high sensitivity and negative predictive value for blaKPC.
First Report of Klebsiella pneumoniae-Carbapenemase-3-Producing Escherichia coli ST479 in Poland.
The study reports the first detection of bla KPC-3 in an E. coli ST479 in Poland, along with the presence of bla TEM-34, indicating carbapenem and beta-lactam resistance.
Genetic Characterization of Carbapenem-Resistant Enterobacteriaceae and the Spread of Carbapenem-Resistant Klebsiella pneumonia ST340 at a University Hospital in Thailand.
The study identified blaKPC-13 and blaIMP-14a as the carbapenemase genes responsible for carbapenem resistance in CRE isolates. blaKPC-13 was found in E. coli, E. cloacae, and C. freundii, while blaIMP-14a was found in K. pneumoniae. Additionally, reduced expression of ompK35 and ompK36 porin genes was associated with increased carbapenem MICs in some CRKP isolates.
Molecular characterization of multidrug-resistant Klebsiella pneumoniae isolates.
The study identified several AMR genes and mutations in multidrug-resistant Klebsiella pneumoniae isolates, including beta-lactamases (bla SHV, bla TEM, bla CTX-M), aminoglycoside resistance genes (aacC1, aacC4, aadA1, strB), carbapenemase (bla KPC-2), and quinolone resistance gene (qnrB). Additionally, a gyrA mutation (S83L) was found to confer quinolone resistance.
Factors Associated to Prevalence and Incidence of Carbapenem-Resistant Enterobacteriaceae Fecal Carriage: A Cohort Study in a Mexican Tertiary Care Hospital.
The study identified OXA-232, CTX-M-15, SHV, NDM-1, KPC-1, and IMP as the primary molecular mechanisms of carbapenem resistance in CRE isolates. These genes were detected through PCR and sequencing.
Clonal Dissemination of Enterobacter cloacae Harboring blaKPC-3 in the Upper Midwestern United States.
The study identifies a clonal lineage of Enterobacter cloacae harboring blaKPC-3, which is responsible for carbapenem resistance. The plasmids carrying blaKPC-3 were found to transfer to Escherichia coli, highlighting the risk of resistance spread.
Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla SHV, bla TEM, bla CTX-M, or bla KPC When Subject to β-Lactam Antibiotics.
The study identified the presence of bla TEM, bla SHV, bla CTX-M, and bla KPC genes in K. pneumoniae isolates, which confer resistance to various β-lactam antibiotics. The isolates exhibited ultrastructural changes when exposed to sub-MICs of these antibiotics.
Genomic Epidemiology of an Endoscope-Associated Outbreak of Klebsiella pneumoniae Carbapenemase (KPC)-Producing K. pneumoniae.
The study identified a novel KPC-encoding plasmid, pKp28, specific to subclade I of ST258 KPC-Kp, which was useful for tracking the outbreak. Additionally, it characterized the genetic diversity and transmission dynamics of ST258 KPC-Kp during an endoscope-associated outbreak.
Evaluation of the BYG Carba Test, a New Electrochemical Assay for Rapid Laboratory Detection of Carbapenemase-Producing Enterobacteriaceae.
The BYG Carba test was evaluated for the rapid detection of carbapenemase-producing Enterobacteriaceae, demonstrating high sensitivity and specificity for detecting blaOXA-48, blaKPC, blaNDM, and blaVIM genes.
Complete Sequence of a Novel IncR-F33:A–:B– Plasmid, pKP1034, Harboring fosA3, blaKPC-2, blaCTX-M-65, blaSHV-12, and rmtB from an Epidemic Klebsiella pneumoniae Sequence Type 11 Strain in China.
The study identifies a novel plasmid pKP1034 carrying multiple resistance genes including fosA3, blaKPC-2, blaCTX-M-65, blaSHV-12, and rmtB in a Klebsiella pneumoniae ST11 strain, highlighting the complex resistance mechanisms and potential for horizontal gene transfer.
Mutational and acquired carbapenem resistance mechanisms in multidrug resistant Pseudomonas aeruginosa clinical isolates from Recife, Brazil.
The study identifies several AMR genes including blaGES-1, blaKPC-2, blaSPM-1, aac(6')-Ib, ant(2')-Ia, aph(3')-VIa, rmtD, catB3, arr-4, qacED1, sul1, and intI1, as well as mutations in the oprD gene contributing to carbapenem resistance in multidrug-resistant P. aeruginosa isolates from Recife, Brazil.
Xpert CARBA-R Assay for the Detection of Carbapenemase-Producing Organisms in Intensive Care Unit Patients of a Korean Tertiary Care Hospital
The Xpert CARBA-R assay detected carbapenemase-producing organisms (CPO) in ICU patients, identifying blaVIM, blaIMP, and blaKPC genes as responsible for carbapenem resistance.
Co-Carriage of blaKPC-2 and blaNDM-1 in Clinical Isolates of Pseudomonas aeruginosa Associated with Hospital Infections from India.
The study identifies the first detection of integron-mediated blaKPC-2 coexisting with blaNDM-1 in Pseudomonas aeruginosa from India, highlighting the emergence of multidrug-resistant isolates carrying these carbapenemase genes.
Coproduction of KPC-18 and VIM-1 Carbapenemases by Enterobacter cloacae: Implications for Newer β-Lactam-β-Lactamase Inhibitor Combinations.
The study identifies the coproduction of KPC-18 and VIM-1 carbapenemases in Enterobacter cloacae strain G6809, highlighting the challenge of treating infections caused by multidrug-resistant organisms with newer β-lactam-β-lactamase inhibitor combinations.
Coproduction of KPC-18 and VIM-1 Carbapenemases by Enterobacter cloacae: Implications for Newer β-Lactam-β-Lactamase Inhibitor Combinations.
Coproduction of KPC-18 and VIM-1 Carbapenemases by Enterobacter cloacae: Implications for Newer β-Lactam-β-Lactamase Inhibitor Combinations.
Coproduction of KPC-18 and VIM-1 Carbapenemases by Enterobacter cloacae: Implications for Newer β-Lactam-β-Lactamase Inhibitor Combinations.
Dissemination of carbapenemases producing Gram negative bacteria in the Middle East.
The study reviews the dissemination of carbapenemase-producing Gram-negative bacteria in the Middle East, highlighting the prevalence of KPC, VIM, IMP, NDM, and OXA-48 enzymes in various bacterial species such as Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii, and Pseudomonas aeruginosa.
Dissemination of carbapenemases producing Gram negative bacteria in the Middle East.
The study reviews the dissemination of carbapenemase-producing Gram-negative bacteria in the Middle East, highlighting the prevalence of KPC, VIM, IMP, NDM, and OXA-48 enzymes in various bacterial species such as Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii, and Pseudomonas aeruginosa.
Dissemination of carbapenemases producing Gram negative bacteria in the Middle East.
The study reviews the dissemination of carbapenemase-producing Gram-negative bacteria in the Middle East, highlighting the prevalence of KPC, VIM, IMP, NDM, and OXA-48 enzymes in various bacterial species such as Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii, and Pseudomonas aeruginosa.
Evaluation of the FilmArray Blood Culture Identification Panel: Results of a Multicenter Controlled Trial.
The FilmArray BCID panel accurately detected methicillin-resistant Staphylococcus aureus (mecA), vancomycin-resistant Enterococcus (vanA), and Klebsiella pneumoniae carbapenemase (blaKPC) in blood cultures with high sensitivity and specificity.
Treatment options for infections caused by carbapenem-resistant Enterobacteriaceae: can we apply "precision medicine" to antimicrobial chemotherapy?
The paper discusses the mechanisms of resistance in carbapenem-resistant Enterobacteriaceae (CRE), focusing on beta-lactamases such as KPC, NDM, and OXA-48, as well as regulatory genes and efflux pumps contributing to polymyxin and tigecycline resistance.
KPC enzymes in the UK: an analysis of the first 160 cases outside the North-West region.
The study characterizes KPC enzymes, specifically blaKPC-2, blaKPC-3, and blaKPC-4, which confer resistance to carbapenems in various Enterobacteriaceae, with Klebsiella pneumoniae ST258 being the dominant host.
KPC enzymes in the UK: an analysis of the first 160 cases outside the North-West region.
The study characterizes KPC enzymes, specifically blaKPC-2, blaKPC-3, and blaKPC-4, which confer resistance to carbapenems in various Enterobacteriaceae, with Klebsiella pneumoniae ST258 being the dominant host.
KPC enzymes in the UK: an analysis of the first 160 cases outside the North-West region.
The study characterizes KPC enzymes, specifically blaKPC-2, blaKPC-3, and blaKPC-4, which confer resistance to carbapenems in various Enterobacteriaceae, with Klebsiella pneumoniae ST258 being the dominant host.
Severe Bloodstream Infection due to KPC-Producer E coli in a Renal Transplant Recipient Treated With the Double-Carbapenem Regimen and Analysis of In Vitro Synergy Testing: A Case Report.
The study describes a case of bloodstream infection caused by a KPC-producing E. coli in a renal transplant recipient, highlighting the effectiveness of a double-carbapenem regimen (ertapenem and meropenem) in vitro against the strain.
Class A Carbapenemases: Mechanisms of Action and Resistance
The paper characterizes various class A carbapenemases, including KPC, GES, SME, NmcA, FRI-1, BIC-1, SFC-1, and PenA, highlighting their roles in conferring resistance to carbapenems and other β-lactam antibiotics.
Class A Carbapenemases: Mechanisms of Action and Resistance
The paper characterizes various class A carbapenemases, including KPC, GES, SME, NmcA, FRI-1, BIC-1, SFC-1, and PenA, highlighting their roles in conferring resistance to carbapenems and other β-lactam antibiotics.
Removal of the Side Chain at the Active-Site Serine by a Glycine Substitution Increases the Stability of a Wide Range of Serine beta-lactamases by Relieving Steric Strain.
The study demonstrates that replacing the active-site serine with glycine in various serine beta-lactamases enhances their thermal stability by reducing steric strain, although this substitution significantly reduces catalytic efficiency.
Characterization and Clinical Impact of Bloodstream Infection Caused by Carbapenemase-Producing Enterobacteriaceae in Seven Latin American Countries.
The study identified carbapenemase-producing Enterobacteriaceae (CPE) in 53 out of 255 patients with bloodstream infections, with bla KPC, bla VIM, and bla NDM being the most prevalent genes. These genes conferred resistance to carbapenems, and CPE infections were associated with higher mortality rates.
Molecular Diversity and Plasmid Analysis of KPC-Producing Escherichia coli.
The study characterized blaKPC-harboring plasmids in 9 carbapenem-resistant E. coli isolates, identifying various plasmid types and highlighting the role of horizontal gene transfer in the spread of KPC resistance.
Multisite Evaluation of Cepheid Xpert Carba-R Assay for Detection of Carbapenemase-Producing Organisms in Rectal Swabs.
The Cepheid Xpert Carba-R assay effectively detects carbapenemase-producing organisms (CPO) by identifying specific carbapenemase genes, including blaIMP-1, blaVIM, blaNDM, blaKPC, and blaOXA-48, in rectal swab specimens.
Diverse Genetic Background of Multidrug-Resistant Pseudomonas aeruginosa from Mainland China, and Emergence of an Extensively Drug-Resistant ST292 Clone in Kunming.
The study identified various carbapenemase genes including blaIMP-9, blaIMP-1, blaIMP-10, blaVIM-2, and blaKPC-2 in multidrug-resistant Pseudomonas aeruginosa isolates from mainland China, highlighting the emergence of an extensively drug-resistant ST292 clone.
Antimicrobial Resistance Mechanisms
The paper discusses various mechanisms of antibiotic resistance, including beta-lactamases like blaKPC, blaNDM, blaIMP, and blaVIM, which confer resistance to carbapenems. It also covers aminoglycoside modifying enzymes such as aac(6')-Ib and aac(6')-I, quinolone resistance proteins like qnr, tetracycline resistance genes such as tet(M) and tet(O), macrolide resistance genes like erm, mefA, and mefE, and efflux pump systems like mexAB-oprM and acrAB-tolC.
Prevalence and Fate of Carbapenemase Genes in a Wastewater Treatment Plant in Northern China.
The study identified the prevalence of bla KPC-2, bla GES-1, and bla IMP-1 carbapenemase genes in a wastewater treatment plant, highlighting their persistence through various treatment stages and potential environmental dissemination.
Synergistic and Additive Effect of Oregano Essential Oil and Biological Silver Nanoparticles against Multidrug-Resistant Bacterial Strains.
Synergistic and Additive Effect of Oregano Essential Oil and Biological Silver Nanoparticles against Multidrug-Resistant Bacterial Strains.
Genetic environment of the KPC gene in Acinetobacter baumannii ST2 clone from Puerto Rico and genomic insights into its drug resistance.
The study identified the blaKPC-2 gene in an Acinetobacter baumannii ST2 clone from Puerto Rico, located in a novel truncated version of Tn4401e (Tn4401h) within the chromosome. Additionally, the study found a chromosomally located truncated Tn1 transposon harboring blaTEM-1 and identified other resistance mechanisms including efflux pumps and mutations in topoisomerase and DNA gyrase genes.
Horizontal Transfer of Carbapenemase-Encoding Plasmids and Comparison with Hospital Epidemiology Data.
The study characterizes the horizontal transfer of carbapenemase-encoding plasmids, focusing on blaKPC genes, and examines factors influencing conjugation efficiency in various bacterial strains.
Performance evaluation of three automated identification systems in detecting carbapenem-resistant Enterobacteriaceae.
The study identified carbapenemase genes blaIMP-4, blaIMP-8, blaNDM-1, and blaKPC-2 in carbapenem-resistant Enterobacteriaceae isolates.
Effect of the β-Lactamase Inhibitor Vaborbactam Combined with Meropenem against Serine Carbapenemase-Producing Enterobacteriaceae.
Vaborbactam, a β-lactamase inhibitor, restores meropenem activity against serine carbapenemase-producing Enterobacteriaceae, particularly those expressing KPC and VIM enzymes.
Performance Evaluation of the IRIDICA BAC BSI Assay for Detection of Bloodstream Infections
The study characterizes the IRIDICA BAC BSI Assay for rapid detection of bacteria and Candida in blood, focusing on the detection of specific antibiotic resistance markers including mecA, vanA, vanB, and blaKPC.
Molecular typing and genetic environment of the blaKPC gene in Chilean isolates of Klebsiella pneumoniae.
Molecular typing and genetic environment of the blaKPC gene in Chilean isolates of Klebsiella pneumoniae.
Molecular typing and genetic environment of the blaKPC gene in Chilean isolates of Klebsiella pneumoniae.
Successful treatment of a disseminated infection with extensively drug-resistant Klebsiella pneumoniae in a liver transplant recipient with a fosfomycin-based multidrug regimen.
The case highlights the effectiveness of a fosfomycin-based regimen in treating a disseminated infection with extensively drug-resistant Klebsiella pneumoniae in a liver transplant recipient, with the detection of the bla KPC-3 allele and wzi -168 sequence.
Citrobacter freundii carrying blaKPC-2 and blaNDM-1: characterization by whole genome sequencing.
The study characterizes a Citrobacter freundii strain carrying both blaKPC-2 and blaNDM-1, which confer high-level carbapenem resistance. Additionally, blaCMY-137, a novel CMY enzyme, was identified and functionally validated.
Carbapenem-resistant Enterobacteriaceae in special populations: Solid organ transplant recipients, stem cell transplant recipients, and patients with hematologic malignancies.
The paper discusses the prevalence and impact of carbapenem-resistant Enterobacteriaceae (CRE) in special populations such as solid organ transplant recipients, stem cell transplant recipients, and patients with hematologic malignancies. It highlights the mechanisms of carbapenem resistance, including the production of KPC, OXA-48, and NDM beta-lactamases.
Use of Cepheid Xpert Carba-R® for Rapid Detection of Carbapenemase-Producing Bacteria in Abdominal Septic Patients Admitted to Intensive Care Unit.
The study evaluates the effectiveness of the Xpert Carba-R assay in detecting carbapenemase-producing bacteria in abdominal septic patients. It identifies several carbapenem resistance genes, including bla KPC, bla VIM, bla OXA-48, bla IMP-1, and bla NDM, which are associated with resistance to carbapenems.
Outbreak by Ventilator-Associated ST11 K. pneumoniae with Co-production of CTX-M-24 and KPC-2 in a SICU of a Tertiary Teaching Hospital in Central China.
The study identifies the co-existence of bla KPC-2 and bla CTX-M-24 in a K. pneumoniae isolate, highlighting the emergence of multidrug-resistant strains in a hospital setting.
Prevalence and Molecular Characteristics of Carbapenemase-Producing Enterobacteriaceae From Five Hospitals in Korea.
The study identified bla_OXA-232, bla_KPC-2, bla_IMP-1, and bla_NDM-1 genes as the primary carbapenemase genes in carbapenemase-producing Enterobacteriaceae (CPE) isolates from five hospitals in Korea.
Molecular epidemiology of carbapenem resistant gram-negative bacilli from infected pediatric population in tertiary - care hospitals in Medellín, Colombia: an increasing problem.
The study identified KPC carbapenemase as the primary resistance mechanism in Klebsiella pneumoniae isolates, with KPC-2 and KPC-3 variants detected. VIM-2 carbapenemase was found in Pseudomonas aeruginosa isolates, and KPC-3 was detected in Enterobacter cloacae.
Insights on the Horizontal Gene Transfer of Carbapenemase Determinants in the Opportunistic Pathogen Acinetobacter baumannii.
The paper discusses the horizontal gene transfer of carbapenemase determinants in Acinetobacter baumannii, focusing on various carbapenemase genes such as bla OXA-23, bla OXA-40, bla OXA-58, bla OXA-143, and bla OXA-235, highlighting their roles in carbapenem resistance and the mechanisms of their dissemination.
Antimicrobial susceptibility testing in predicting the presence of carbapenemase genes in Enterobacteriaceae in South Africa.
The study identified bla_NDM, bla_OXA-48, bla_VIM, bla_IMP, bla_GES, and bla_KPC as the most prevalent carbapenemase genes in Enterobacteriaceae isolates in South Africa, with bla_NDM being the most common.
beta-lactamases: A Focus on Current Challenges.
The paper discusses the mechanisms of action and resistance of beta-lactamases, focusing on clinically important enzymes such as KPC, NDM, and OXA, which confer resistance to various β-lactam antibiotics.
Engineering Specificity from Broad to Narrow: Design of a beta-lactamase Inhibitory Protein (BLIP) Variant That Exclusively Binds and Detects KPC beta-lactamase.
The study engineered a BLIP variant (K74T:W112D) that specifically binds and detects KPC-2 beta-lactamase with high affinity while showing reduced affinity for other class A beta-lactamases like TEM-1.
A cluster of KPC-2 and VIM-2-producing Klebsiella pneumoniae ST833 isolates from the pediatric service of a Venezuelan Hospital.
All 19 isolates contained both bla KPC-2 and bla VIM-2 genes, which encode serine and metallo-beta-lactamases, respectively. Additionally, the qac DeltaE and add A2 genes were detected, indicating resistance to quaternary ammonium compounds and streptomycin.
Population Screening Using Sewage Reveals Pan-Resistant Bacteria in Hospital and Community Samples.
The study identified blaKPC and blaNDM-1 genes in pan-resistant bacteria found in Israeli sewage, highlighting the prevalence of carbapenem-resistant Enterobacteriaceae, MRSA, and VRE in both hospital and community sewage.
Detection of KPC Carbapenemase in Pseudomonas aeruginosa Isolated From Clinical Samples Using Modified Hodge Test and Boronic Acid Phenotypic Methods and Their Comparison With the Polymerase Chain Reaction.
The study identified 13 Pseudomonas aeruginosa isolates (12%) positive for the blaKPC gene, indicating KPC carbapenemase production. The boronic acid phenotypic method showed higher sensitivity than the Modified Hodge Test for detecting KPC-producing strains.
A prospective study of treatment of carbapenem-resistant Enterobacteriaceae infections and risk factors associated with outcome.
The study identified the bla KPC gene as the primary carbapenemase in carbapenem-resistant Enterobacteriaceae (CRE) isolates, highlighting its prevalence and association with high mortality rates in patients with CRE infections.
Enterobacteriaceae Isolated from the River Danube: Antibiotic Resistances, with a Focus on the Presence of ESBL and Carbapenemases.
The study identified ESBL genes such as blaCTX-M-15, blaNDM-1, and blaKPC-2 in Enterobacteriaceae isolated from the River Danube, highlighting the presence of multidrug-resistant bacteria in aquatic environments.
Identification of Klebsiella pneumoniae Carbapenemase-producing Klebsiella oxytoca in Clinical Isolates in Tehran Hospitals, Iran by Chromogenic Medium and Molecular Methods.
The study identified a multidrug-resistant K. oxytoca isolate carrying the blaKPC gene, which was confirmed by PCR and CHROMagar KPC chromogenic culture media. The isolate exhibited resistance to multiple antibiotics, including carbapenems, cephalosporins, and fluoroquinolones.
Antimicrobial resistance in Enterobacteriaceae in Brazil: focus on β-lactams and polymyxins.
The paper discusses the emergence and spread of various beta-lactamases, including CTX-M, KPC-2, and NDM-1, as well as polymyxin resistance mechanisms in Enterobacteriaceae in Brazil.
Direct identification of antibiotic resistance genes on single plasmid molecules using CRISPR/Cas9 in combination with optical DNA mapping.
The study presents a novel method using CRISPR/Cas9 and optical DNA mapping to identify antibiotic resistance genes on single plasmid molecules. It successfully identifies bla CTX-M-15, bla CTX-M-14, bla KPC, and bla NDM genes in various bacterial isolates.
The rapid spread of carbapenem-resistant Enterobacteriaceae.
The paper discusses the rapid spread of carbapenem-resistant Enterobacteriaceae (CRE) and characterizes various carbapenemase genes such as bla KPC, bla NDM, bla VIM, bla OXA-48, and bla IMP, highlighting their roles in conferring resistance to carbapenems.
The rapid spread of carbapenem-resistant Enterobacteriaceae.
The paper discusses the rapid spread of carbapenem-resistant Enterobacteriaceae (CRE) and characterizes various carbapenemase genes such as bla KPC, bla NDM, bla VIM, bla OXA-48, and bla IMP, highlighting their roles in conferring resistance to carbapenems.
The rapid spread of carbapenem-resistant Enterobacteriaceae.
The paper discusses the rapid spread of carbapenem-resistant Enterobacteriaceae (CRE) and characterizes various carbapenemase genes such as bla KPC, bla NDM, bla VIM, bla OXA-48, and bla IMP, highlighting their roles in conferring resistance to carbapenems.
The rapid spread of carbapenem-resistant Enterobacteriaceae.
The paper discusses the rapid spread of carbapenem-resistant Enterobacteriaceae (CRE) and characterizes various carbapenemase genes such as bla KPC, bla NDM, bla VIM, bla OXA-48, and bla IMP, highlighting their roles in conferring resistance to carbapenems.
A Prolonged Outbreak of KPC-3-Producing Enterobacter cloacae and Klebsiella pneumoniae Driven by Multiple Mechanisms of Resistance Transmission at a Large Academic Burn Center.
The study identifies bla KPC-3 as a key gene responsible for carbapenem resistance in Enterobacter cloacae and Klebsiella pneumoniae during a prolonged outbreak, highlighting the role of plasmid-mediated transmission and transposon elements in the spread of resistance.
Mechanisms of Evolution in High-Consequence Drug Resistance Plasmids.
The study identifies the blaKPC gene and aac(6')-Ib as key contributors to carbapenem and aminoglycoside resistance in Enterobacteriaceae, highlighting the role of plasmid rearrangements and transposable elements in the evolution of resistance.
Comprehensive Genome Analysis of Carbapenemase-Producing Enterobacter spp.: New Insights into Phylogeny, Population Structure, and Resistance Mechanisms.
The study identifies multiple carbapenemase genes (bla KPC-2, bla KPC-3, bla KPC-4, and bla NDM-1) and other resistance genes (such as qnrB19, qnrB2, qnrS1, bla TEM-1A, bla TEM-1B, bla OXA-9, bla SHV-12, aadA2, aac(6')-Ib, aac(6')-Ib-cr, aph(3')-Ia, aph(3')-Ic, strA, strB, sul1, sul2, dfrA14, dfrA18, mph(A), catB3, arr-3, and tet(D)) in carbapenem-resistant Enterobacter spp. These genes are primarily located on plasmids and contribute to multidrug resistance.
Comprehensive Genome Analysis of Carbapenemase-Producing Enterobacter spp.: New Insights into Phylogeny, Population Structure, and Resistance Mechanisms.
The study identifies multiple carbapenemase genes (bla KPC-2, bla KPC-3, bla KPC-4, and bla NDM-1) and other resistance genes (such as qnrB19, qnrB2, qnrS1, bla TEM-1A, bla TEM-1B, bla OXA-9, bla SHV-12, aadA2, aac(6')-Ib, aac(6')-Ib-cr, aph(3')-Ia, aph(3')-Ic, strA, strB, sul1, sul2, dfrA14, dfrA18, mph(A), catB3, arr-3, and tet(D)) in carbapenem-resistant Enterobacter spp. These genes are primarily located on plasmids and contribute to multidrug resistance.
Comprehensive Genome Analysis of Carbapenemase-Producing Enterobacter spp.: New Insights into Phylogeny, Population Structure, and Resistance Mechanisms.
The study identifies multiple carbapenemase genes (bla KPC-2, bla KPC-3, bla KPC-4, and bla NDM-1) and other resistance genes (such as qnrB19, qnrB2, qnrS1, bla TEM-1A, bla TEM-1B, bla OXA-9, bla SHV-12, aadA2, aac(6')-Ib, aac(6')-Ib-cr, aph(3')-Ia, aph(3')-Ic, strA, strB, sul1, sul2, dfrA14, dfrA18, mph(A), catB3, arr-3, and tet(D)) in carbapenem-resistant Enterobacter spp. These genes are primarily located on plasmids and contribute to multidrug resistance.
Comprehensive Genome Analysis of Carbapenemase-Producing Enterobacter spp.: New Insights into Phylogeny, Population Structure, and Resistance Mechanisms.
The study identifies multiple carbapenemase genes (bla KPC-2, bla KPC-3, bla KPC-4, and bla NDM-1) and other resistance genes (such as qnrB19, qnrB2, qnrS1, bla TEM-1A, bla TEM-1B, bla OXA-9, bla SHV-12, aadA2, aac(6')-Ib, aac(6')-Ib-cr, aph(3')-Ia, aph(3')-Ic, strA, strB, sul1, sul2, dfrA14, dfrA18, mph(A), catB3, arr-3, and tet(D)) in carbapenem-resistant Enterobacter spp. These genes are primarily located on plasmids and contribute to multidrug resistance.
Epidemiology and biomolecular characterization of carbapenem-resistant klebsiella pneumoniae in an Italian hospital.
The study identified blaKPC as the most frequent carbapenemase in carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates, with KPC-3 being the dominant variant.
Evaluation of the Xpert Carba-R (Cepheid) Assay Using Contrived Bronchial Specimens from Patients with Suspicion of Ventilator-Associated Pneumonia for the Detection of Prevalent Carbapenemases.
The Xpert Carba-R assay effectively detects carbapenemase-producing organisms directly in contrived bronchial aspirates, demonstrating 100% sensitivity and specificity for the detection of bla KPC, bla NDM, bla VIM, bla OXA-48, and bla IMP-1 genes.
Emergence of Ceftazidime-Avibactam Resistance Due to Plasmid-Borne blaKPC-3 Mutations during Treatment of Carbapenem-Resistant Klebsiella pneumoniae Infections.
The study identifies blaKPC-3 mutations as the cause of ceftazidime-avibactam resistance in carbapenem-resistant Klebsiella pneumoniae, with specific mutations (D179Y, T243M, V240G) leading to reduced susceptibility to multiple beta-lactam antibiotics.
Emergence of Ceftazidime-Avibactam Resistance Due to Plasmid-Borne bla(KPC-3) Mutations during Treatment of Carbapenem-Resistant Klebsiella pneumoniae Infections.
Emergence of Ceftazidime-Avibactam Resistance Due to Plasmid-Borne bla(KPC-3) Mutations during Treatment of Carbapenem-Resistant Klebsiella pneumoniae Infections.
Emergence of Ceftazidime-Avibactam Resistance Due to Plasmid-Borne bla(KPC-3) Mutations during Treatment of Carbapenem-Resistant Klebsiella pneumoniae Infections.
Emergence of Ceftazidime-Avibactam Resistance Due to Plasmid-Borne bla(KPC-3) Mutations during Treatment of Carbapenem-Resistant Klebsiella pneumoniae Infections.
Emergence of Ceftazidime-Avibactam Resistance Due to Plasmid-Borne bla(KPC-3) Mutations during Treatment of Carbapenem-Resistant Klebsiella pneumoniae Infections.
Emergence of Ceftazidime-Avibactam Resistance Due to Plasmid-Borne bla(KPC-3) Mutations during Treatment of Carbapenem-Resistant Klebsiella pneumoniae Infections.
Chromosomal Integration of the Klebsiella pneumoniae Carbapenemase Gene, bla(KPC), in Klebsiella Species Is Elusive but Not Rare.
The study identifies chromosomal integration of the blaKPC gene in Klebsiella species, highlighting that this is not a rare event and can be missed by conventional phenotypic methods.
Comparison of phenotypic and PCR methods for detection of carbapenemases production by Enterobacteriaceae.
The study identified the presence of various carbapenemase genes, including blaIMP, blaVIM, blaNDM, blaKPC, and blaOXA-48, in Enterobacteriaceae isolates, highlighting the importance of molecular methods for rapid and accurate detection of carbapenemase-producing organisms.
Antimicrobial Activity of Ceftazidime-Avibactam against Gram-Negative Bacteria Isolated from Patients Hospitalized with Pneumonia in U.S. Medical Centers, 2011 to 2015.
Ceftazidime-avibactam showed high activity against Gram-negative bacteria, including those with resistance mechanisms such as KPC, CTX-M-15, and NDM-1 beta-lactamases.
The emergence of carbapenem resistant Klebsiella pneumoniae in Malaysia: correlation between microbiological trends with host characteristics and clinical factors.
The study identified several carbapenemase genes, including bla_OXA-48, bla_KPC-2, bla_IMP-8, bla_NDC-1, and bla_NMC-A, in carbapenem-resistant Klebsiella pneumoniae strains isolated in Malaysia. These genes were associated with resistance to carbapenems and other antibiotics.
Comparison of 11 Phenotypic Assays for Accurate Detection of Carbapenemase-Producing Enterobacteriaceae
The study evaluates 11 phenotypic assays for the detection of carbapenemase-producing Enterobacteriaceae (CPE) and identifies the effectiveness of various assays in detecting different types of carbapenemase genes, including bla KPC, bla NDM, bla OXA-48-type, bla VIM, bla IMP, bla SME, and bla IMI.
Development of an algorithm for phenotypic screening of carbapenemase-producing Enterobacteriaceae in the routine laboratory.
The study identifies OXA-48 group carbapenemase, KPC, and MBL as the primary carbapenemase types among carbapenem non-susceptible Enterobacteriaceae isolates, highlighting the need for targeted screening strategies.
Antibacterial Resistance in Patients with Hematopoietic Stem Cell Transplantation.
The paper discusses the challenges of antibacterial resistance in patients undergoing hematopoietic stem cell transplantation (HSCT), highlighting the emergence of multidrug-resistant bacterial infections and the need for tailored empirical antibacterial therapy based on local epidemiology.
Antibacterial Resistance in Patients with Hematopoietic Stem Cell Transplantation.
The paper discusses the challenges of antibacterial resistance in patients undergoing hematopoietic stem cell transplantation (HSCT), highlighting the emergence of multidrug-resistant bacterial infections and the need for tailored empirical antibacterial therapy based on local epidemiology.
A putative multi-replicon plasmid co-harboring beta-lactamase genes blaKPC-2, blaCTX-M-14 and blaTEM-1 and trimethoprim resistance gene dfrA25 from a Klebsiella pneumoniae sequence type (ST) 11 strain in China.
The study identifies a novel multi-replicon plasmid pHS091147 carrying multiple resistance genes, including blaKPC-2, blaCTX-M-14, blaTEM-1, dfrA25, and sul1, which confer resistance to various antibiotics such as carbapenems, cephalosporins, and trimethoprim.
Carbapenem MICs in Escherichia coli and Klebsiella Species Producing Extended-Spectrum β-Lactamases in Critical Care Patients from 2001 to 2009.
The study identified blaKPC as a gene conferring resistance to multiple carbapenems in ESBL-producing E. coli and Klebsiella species.
Molecular Characteristics of Carbapenem-Resistant Enterobacter cloacae in Ningxia Province, China.
The study identified blaNDM-1, blaKPC, blaIMP, and blaVIM as the main carbapenem resistance genes in carbapenem-resistant Enterobacter cloacae isolates from Ningxia, China. These genes were found in multiple isolates and could be transferred to Escherichia coli via conjugation.
Mutations in bla KPC-3 That Confer Ceftazidime-Avibactam Resistance Encode Novel KPC-3 Variants That Function as Extended-Spectrum beta-lactamases.
Mutations in blaKPC-3, including D179Y, T243M, D179Y/T243M, and EL165-166, confer resistance to ceftazidime-avibactam and alter susceptibility to carbapenems and other β-lactams.
Mutations in bla(KPC-3) That Confer Ceftazidime-Avibactam Resistance Encode Novel KPC-3 Variants That Function as Extended-Spectrum β-Lactamases.
Mutations in bla(KPC-3) That Confer Ceftazidime-Avibactam Resistance Encode Novel KPC-3 Variants That Function as Extended-Spectrum β-Lactamases.
Mutations in bla(KPC-3) That Confer Ceftazidime-Avibactam Resistance Encode Novel KPC-3 Variants That Function as Extended-Spectrum β-Lactamases.
Development of a Rapid Reverse Blot Hybridization Assay for Detection of Clinically Relevant Antibiotic Resistance Genes in Blood Cultures Testing Positive for Gram-Negative Bacteria.
The study developed and evaluated the REBA-EAC assay for the rapid detection of clinically relevant antibiotic resistance genes in blood cultures positive for Gram-negative bacteria. The assay successfully identified various beta-lactamase genes, including ESBLs (CTX-M, TEM, SHV), AmpC beta-lactamases (DHA, CMY-2-like, ACT), and carbapenemases (IMP, VIM, NDM, KPC, OXA-48-like, SPM).
WCK 5222 (Cefepime-Zidebactam) Antimicrobial Activity against Clinical Isolates of Gram-Negative Bacteria Collected Worldwide in 2015.
The study evaluated the antimicrobial activity of cefepime-zidebactam against clinical isolates of Gram-negative bacteria, highlighting the effectiveness against carbapenem-resistant strains carrying blaNDM-1 and blaKPC-2 genes.
Rapid detection of carbapenemase-producing Klebsiella pneumoniae strains derived from blood cultures by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS).
The study demonstrates the rapid detection of carbapenemase-producing Klebsiella pneumoniae strains using MALDI-TOF MS, identifying various carbapenemase genes including bla KPC, bla NDM, bla OXA-48, bla VIM, bla IMP, bla SHV, bla TEM, and bla CTX-M.
Detection and analysis of different interactions between resistance mechanisms and carbapenems in clinical isolates of Klebsiella pneumoniae.
The study identified the blaKPC gene as the primary mechanism of carbapenem resistance in 89% of the K. pneumoniae isolates, along with porin loss contributing to resistance.
Use of Ancillary Carbapenemase Tests To Improve Specificity of Phenotypic Definitions for Carbapenemase-Producing Enterobacteriaceae.
The study evaluated the performance of ancillary carbapenemase tests, such as the Xpert Carba-R and the carbapenem inactivation method (CIM), to improve the specificity of phenotypic definitions for carbapenemase-producing Enterobacteriaceae (CPE). It identified various carbapenemase genes, including bla IMP, bla KPC, bla NDM, and bla OXA-48-like, and their association with resistance to carbapenems.
Enhanced Klebsiella pneumoniae Carbapenemase Expression from a Novel Tn4401 Deletion
The study identifies a novel Tn4401 isoform, Tn4401 h, with an 188-bp deletion in the noncoding region upstream of blaKPC, leading to increased KPC expression and resistance to carbapenems.
The Epidemiology of Carbapenem-Resistant Enterobacteriaceae: The Impact and Evolution of a Global Menace.
The paper discusses the global spread and mechanisms of carbapenem resistance in Enterobacteriaceae, highlighting the role of various beta-lactamase genes such as blaKPC, blaNDM, blaVIM, blaIMP, and blaOXA-48 in conferring resistance to carbapenems.
Carbapenemase-producing enterobacteriaceae recovered from a Spanish river ecosystem.
The study identified carbapenemase-producing Enterobacteriaceae (CPE) in a river ecosystem, including KPC-2, VIM-1, and IMI-2 carbapenemases. These enzymes were found in various species such as Escherichia coli, Enterobacter cloacae, Klebsiella pneumoniae, Klebsiella oxytoca, and Raoultella ornithinolytica.
Evaluation of a modified meropenem hydrolysis assay on a large cohort of KPC and VIM carbapenemase-producing Enterobacteriaceae.
The study evaluates a modified meropenem hydrolysis assay (MHA) for the detection of carbapenemase-producing Enterobacteriaceae, demonstrating its effectiveness in identifying KPC, VIM, NDM, and OXA-48 carbapenemases.
Impaired Inhibition by Avibactam and Resistance to the Ceftazidime-Avibactam Combination Due to the D179Y Substitution in the KPC-2 beta-lactamase.
Nationwide Surveillance of Clinical Carbapenem-resistant Enterobacteriaceae (CRE) Strains in China.
The study identified blaKPC-2 and blaNDM as the primary carbapenemase genes responsible for carbapenem resistance in CRE in China, with blaKPC-2 being more prevalent in K. pneumoniae and blaNDM in E. coli. Additionally, fosA3 was found to mediate fosfomycin resistance, and mcr-1 was associated with colistin resistance in some E. coli strains.
Population Genomic Analysis of 1,777 Extended-Spectrum Beta-Lactamase-Producing Klebsiella pneumoniae Isolates, Houston, Texas: Unexpected Abundance of Clonal Group 307.
The study identifies the widespread presence of CG307 strains of K. pneumoniae, which exhibit high levels of antimicrobial resistance, particularly through the presence of various beta-lactamase genes such as bla KPC-2, bla NDM-1, bla CTX-M, and bla TEM.
Increasing proportion of carbapenemase-producing Enterobacteriaceae and emergence of a MCR-1 producer through a multicentric study among hospital-based and private laboratories in Belgium from September to November 2015.
The study identified an increased proportion of carbapenemase-producing Enterobacteriaceae (CPE) in Belgium, with OXA-48 being the most prevalent carbapenemase. A plasmid-mediated mcr-1 gene was detected in a colistin-resistant E. coli isolate.
Antimicrobial activity evaluation and comparison of methods of susceptibility for Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacter spp. isolates.
The study evaluated the antimicrobial activity against KPC-producing Enterobacter spp. isolates and found that blaKPC-2 was responsible for carbapenem resistance.
Outbreak by Hypermucoviscous Klebsiella pneumoniae ST11 Isolates with Carbapenem Resistance in a Tertiary Hospital in China.
The study identifies bla KPC-2, bla SHV-11, and bla CTX-M-65 as the primary resistance mechanisms in carbapenem-resistant hypermucoviscous Klebsiella pneumoniae (HMKP) isolates, highlighting the role of these genes in mediating resistance to multiple antibiotics.
Peptide Markers for Rapid Detection of KPC Carbapenemase by LC-MS/MS.
The study identifies and validates three high-abundance, specific tryptic peptides of the KPC carbapenemase protein for rapid detection using LC-MS/MS.
Identifying Spectra of Activity and Therapeutic Niches for Ceftazidime-Avibactam and Imipenem-Relebactam against Carbapenem-Resistant Enterobacteriaceae.
The study identified mutations in the bla KPC-3 gene that confer resistance to ceftazidime-avibactam, highlighting the role of KPC-3 variants in reducing susceptibility to this combination therapy.
Multidrug Resistance Mechanisms of Carbapenem Resistant Klebsiella pneumoniae Strains Isolated in Chongqing, China.
The study identified the high prevalence of blaNDM-1 and blaKPC-2 genes in carbapenem-resistant Klebsiella pneumoniae isolates, along with various quinolone and aminoglycoside resistance genes such as qnrB, aac(6')-Ib, rmtB, qnrS, and acc(6')-Ib-cr.
Molecular Epidemiology and Risk Factors of Carbapenem-Resistant Klebsiella pneumoniae Infections in Eastern China.
The study identified blaKPC-2 as the predominant carbapenemase gene in carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates, contributing to resistance against carbapenems.
Dissemination of Carbapenem-resistant Klebsiella pneumoniae clinical isolates with various combinations of Carbapenemases (KPC-2, NDM-1, NDM-4, and OXA-48) and 16S rRNA Methylases (RmtB and RmtC) in Vietnam.
The study identified carbapenem-resistant Klebsiella pneumoniae isolates carrying various combinations of carbapenemases (KPC-2, NDM-1, NDM-4, OXA-48) and 16S rRNA methylases (RmtB, RmtC) in Vietnam.
Comparison of in-house and commercial real time-PCR based carbapenemase gene detection methods in Enterobacteriaceae and non-fermenting gram-negative bacterial isolates.
The study established and evaluated an in-house multiplex RT-PCR assay for the detection of carbapenemase genes in gram-negative bacteria, demonstrating its effectiveness in identifying blaKPC, blaNDM, blaVIM, and various blaOXA variants.
Molecular detection of beta-lactamase and integron genes in clinical strains of Klebsiella pneumoniae by multiplex polymerase chain reaction.
The study identified the presence of various beta-lactamase genes (blaTEM, blaCTX-M, blaSHV, blaPER, blaGES, blaVIM, blaIMP, blaOXA, blaKPC) and integron genes (intI, intII, intIII) in clinical strains of Klebsiella pneumoniae, highlighting the prevalence of multidrug resistance.
Insights into a Novel bla(KPC-2)-Encoding IncP-6 Plasmid Reveal Carbapenem-Resistance Circulation in Several Enterobacteriaceae Species from Wastewater and a Hospital Source in Spain.
The study identifies a novel IncP-6 plasmid carrying the blaKPC-2 gene, which confers carbapenem resistance in various Enterobacteriaceae species from wastewater and a hospital source in Spain.
Genomic characterization of novel IncFII-type multidrug resistant plasmids p0716-KPC and p12181-KPC from Klebsiella pneumoniae.
The study characterizes two novel IncFII-type multidrug-resistant plasmids, p0716-KPC and p12181-KPC, from Klebsiella pneumoniae. These plasmids carry multiple resistance genes, including blaKPC-2, mph(A), strAB, aacC2, qacEΔ1, sul1, sul2, dfrA25, aphA1a, and blaTEM-1, contributing to resistance against various antibiotics.
Genomic epidemiology of global Klebsiella pneumoniae carbapenemase (KPC)-producing Escherichia coli.
The study characterizes the genetic features of blaKPC emergence in global E. coli, identifying diverse plasmid and mobile genetic element structures associated with carbapenem resistance.
Resistance to Ceftazidime-Avibactam Is Due to Transposition of KPC in a Porin-Deficient Strain of Klebsiella pneumoniae with Increased Efflux Activity.
The study identifies the role of blaKPC-3 and blaSHV-12 beta-lactamase genes, along with the T333N mutation in OmpK36, in conferring resistance to ceftazidime-avibactam in a porin-deficient strain of Klebsiella pneumoniae with increased efflux activity.
Emergence of carbapenemase-producing urinary isolates at a tertiary care hospital in Dhaka, Bangladesh.
The study identified the presence of various carbapenemase genes, including bla NDM-1, bla OXA-48, bla OXA-181, bla KPC, bla VIM, and bla IMP, in carbapenem-resistant Gram-negative uropathogens in Bangladesh.
Whole-Genome Sequencing of Human Clinical Klebsiella pneumoniae Isolates Reveals Misidentification and Misunderstandings of Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae.
The study identifies KPC and NDM-1 beta-lactamase genes in Klebsiella variicola and Klebsiella quasipneumoniae, highlighting their role in multidrug resistance.
Translocation of Carbapenemase Gene blaKPC-2 both Internal and External to Transposons Occurs via Novel Structures of Tn1721 and Exhibits Distinct Movement Patterns.
The study demonstrates that Tn1721-like transposons can transfer the blaKPC-2 gene both internally and externally, revealing distinct movement patterns and transposition frequencies. The blaKPC-2 gene, encoding a carbapenemase, is responsible for carbapenem resistance in Enterobacteriaceae.
Evolving beta-lactamase epidemiology in Enterobacteriaceae from Italian nationwide surveillance, October 2013: KPC-carbapenemase spreading among outpatients.
The study identified the prevalence of beta-lactamase genes, including bla CTX-M-1, bla CTX-M-9, bla KPC, bla VIM, bla OXA-48, and bla NDM, in Enterobacteriaceae isolates from Italy. It highlighted the increasing spread of CTX-M-type enzymes and KPC-carbapenemase among outpatients.
Klebsiella pneumoniae (ST1519) producing KPC-19 carbapenemase in a patient undergoing selective digestive decontamination before liver transplantation.
A novel KPC-19 carbapenemase was identified in a multidrug-resistant Klebsiella pneumoniae strain (ST1519) isolated from a patient undergoing liver transplantation.
Klebsiella pneumoniae (ST1519) producing KPC-19 carbapenemase in a patient undergoing selective digestive decontamination before liver transplantation.
Klebsiella pneumoniae (ST1519) producing KPC-19 carbapenemase in a patient undergoing selective digestive decontamination before liver transplantation.
Klebsiella pneumoniae (ST1519) producing KPC-19 carbapenemase in a patient undergoing selective digestive decontamination before liver transplantation.
Vaborbactam: Spectrum of Beta-Lactamase Inhibition and Impact of Resistance Mechanisms on Activity in Enterobacteriaceae.
Vaborbactam is a broad-spectrum beta-lactamase inhibitor that effectively restores the activity of meropenem against KPC-producing Enterobacteriaceae. It inhibits class A carbapenemases such as KPC-2 and KPC-3, as well as other class A and class C beta-lactamases.
Vaborbactam: Spectrum of Beta-Lactamase Inhibition and Impact of Resistance Mechanisms on Activity in Enterobacteriaceae.
Vaborbactam is a broad-spectrum beta-lactamase inhibitor that effectively restores the activity of meropenem against KPC-producing Enterobacteriaceae. It inhibits class A carbapenemases such as KPC-2 and KPC-3, as well as other class A and class C beta-lactamases.
Low Prevalence of carbapenem-resistant Enterobacteriaceae among wounded military personnel.
The study identified 16 carbapenem-resistant Enterobacteriaceae isolates, including 5 carbapenemase-producing K. pneumoniae isolates carrying blaKPC-3 and one isolate carrying blaNDM-1.
Covert dissemination of carbapenemase-producing Klebsiella pneumoniae (KPC) in a successfully controlled outbreak: long- and short-read whole-genome sequencing demonstrate multiple genetic modes of transmission.
The study identified blaKPC-2 as the primary carbapenem resistance gene in KPC-producing Klebsiella pneumoniae isolates, highlighting the role of plasmid-mediated transmission in the outbreak.
Simple, rapid, and cost-effective modified Carba NP test for carbapenemase detection among Gram-negative bacteria.
The study evaluated modified Carba NP (mCNP) test for rapid detection of carbapenemases among Gram-negative bacteria. It identified bla NDM, bla IMP, bla VIM, bla OXA-48-like, and bla KPC genes as prevalent carbapenemase genes in carbapenem-resistant isolates.
Concentration and Variety of Carbapenemase Producers in Recreational Coastal Waters Showing Distinct Levels of Pollution.
Concentration and Variety of Carbapenemase Producers in Recreational Coastal Waters Showing Distinct Levels of Pollution.
Concentration and Variety of Carbapenemase Producers in Recreational Coastal Waters Showing Distinct Levels of Pollution.
Concentration and Variety of Carbapenemase Producers in Recreational Coastal Waters Showing Distinct Levels of Pollution.
High-Stringency Evaluation of the Automated BD Phoenix CPO Detect and Rapidec Carba NP Tests for Detection and Classification of Carbapenemases.
The study evaluated the BD Phoenix CPO Detect and Rapidec Carba NP tests for detecting and classifying carbapenemases. Both tests showed high sensitivity for carbapenemase detection, with the BD Phoenix CPO Detect also providing classification of carbapenemases.
A rapid diagnostic workflow for cefotaxime-resistant Escherichia coli and Klebsiella pneumoniae detection from blood cultures by MALDI-TOF mass spectrometry.
The study developed a rapid diagnostic workflow using MALDI-TOF MS to detect cefotaxime-resistant Escherichia coli and Klebsiella pneumoniae from blood cultures, identifying beta-lactamase genes such as bla CTX-M, bla KPC, bla SHV, and bla TEM as key contributors to resistance.
Meropenem-Vaborbactam Resistance Selection, Resistance Prevention, and Molecular Mechanisms in Mutants of KPC-Producing Klebsiella pneumoniae.
This study identifies mechanisms of resistance development to meropenem-vaborbactam in KPC-producing K. pneumoniae, highlighting the role of ompK36 inactivation and increased blaKPC copy number.
Virulence Characteristics of Carbapenem-Resistant Klebsiella pneumoniae Strains from Patients with Necrotizing Skin and Soft Tissue Infections.
The study identified carbapenem-resistant Klebsiella pneumoniae (CR-KP) strains with hypervirulent characteristics, including the presence of blaKPC-2 and blaKPC-3 carbapenemase genes, and various virulence factors such as rmpA, rmpA2, and genes involved in iron acquisition and capsule production.
Virulence Characteristics of Carbapenem-Resistant Klebsiella pneumoniae Strains from Patients with Necrotizing Skin and Soft Tissue Infections.
The study identified carbapenem-resistant Klebsiella pneumoniae (CR-KP) strains with hypervirulent characteristics, including the presence of blaKPC-2 and blaKPC-3 carbapenemase genes, and various virulence factors such as rmpA, rmpA2, and genes involved in iron acquisition and capsule production.
Extensively Drug-Resistant Escherichia coli Sequence Type 1642 Carrying an IncX3 Plasmid Containing the blaKPC-2 Gene Associated with Transposon Tn4401a.
The study identifies several AMR genes in an extensively drug-resistant E. coli ST1642 isolate, including blaKPC-2, blaSHV-11, blaTEM-1, aadA5, strA, strB, aac(3)-IId, mph(A), sul1, sul2, tet(B), dfrA17, and qnrS1, which contribute to resistance against multiple antibiotics.
Klebsiella pneumoniae Carbapenemase-2 (KPC-2), Substitutions at Ambler Position Asp179, and Resistance to Ceftazidime-Avibactam: Unique Antibiotic-Resistant Phenotypes Emerge from beta-lactamase Protein Engineering.
The study identifies the KPC-2 Asp179Asn variant as a beta-lactamase that exhibits resistance to ceftazidime-avibactam and other β-lactams due to structural alterations affecting substrate hydrolysis and trapping.
Multicenter Evaluation of the Modified Carbapenem Inactivation Method and the Carba NP for Detection of Carbapenemase-Producing Pseudomonas aeruginosa and Acinetobacter baumannii.
The study characterizes various carbapenemase genes, including blaVIM, blaIMP, blaKPC, blaNDM, blaSPM, blaOXA-23, blaOXA-24/40, blaOXA-58, blaOXA-72, and blaOXA-237, which confer resistance to carbapenems in Pseudomonas aeruginosa and Acinetobacter baumannii.
Comparison of Clinical & Laboratory Standards Institute standards in antimicrobial susceptibility among the carbapenemase producing Enterobacteriaceae.
The study found that 83.3% of the carbapenem-resistant Enterobacteriaceae isolates harbored the blaKPC gene, which confers resistance to carbapenems such as ertapenem, imipenem, and meropenem.
Molecular and epidemiological characterization of carbapenemase-producing Enterobacteriaceae in Norway, 2007 to 2014.
The study identified various carbapenemase genes including bla KPC, bla NDM, bla OXA-48-like, bla VIM, bla IMI, and bla IMP in different Enterobacteriaceae species. These genes conferred resistance to carbapenems. Additionally, mutations in pmrA and pmrB were associated with colistin resistance in Klebsiella pneumoniae.
Occurrence of Extended Spectrum β-Lactamases, KPC-Type, and MCR-1.2-Producing Enterobacteriaceae from Wells, River Water, and Wastewater Treatment Plants in Oltrepò Pavese Area, Northern Italy.
The study identified various extended spectrum beta-lactamase (ESBL) genes, including bla CTX-M-1, bla CTX-M-14, bla CTX-M-15, bla CTX-M-28, and bla CTX-M-138, as well as carbapenemase genes bla KPC-2 and bla KPC-3, and the colistin resistance gene mcr-1.2 in Enterobacteriaceae isolated from water sources in the Oltrepò Pavese area. These genes were found to confer resistance to multiple antibiotics, highlighting the presence of multidrug-resistant bacteria in the environment.
Occurrence of Extended Spectrum β-Lactamases, KPC-Type, and MCR-1.2-Producing Enterobacteriaceae from Wells, River Water, and Wastewater Treatment Plants in Oltrepò Pavese Area, Northern Italy.
The study identified various extended spectrum beta-lactamase (ESBL) genes, including bla CTX-M-1, bla CTX-M-14, bla CTX-M-15, bla CTX-M-28, and bla CTX-M-138, as well as carbapenemase genes bla KPC-2 and bla KPC-3, and the colistin resistance gene mcr-1.2 in Enterobacteriaceae isolated from water sources in the Oltrepò Pavese area. These genes were found to confer resistance to multiple antibiotics, highlighting the presence of multidrug-resistant bacteria in the environment.
Characterization of Metagenomes in Urban Aquatic Compartments Reveals High Prevalence of Clinically Relevant Antibiotic Resistance Genes in Wastewaters.
The study reveals high prevalence of clinically relevant antibiotic resistance genes in wastewater, particularly in hospital discharge samples, including bla KPC-2, bla OXA, bla NDM, qnrS1, qnrS2, and aph(3"). These genes are associated with resistance to carbapenems, beta-lactams, quinolones, and aminoglycosides.
Antimicrobial Resistance of Hypervirulent Klebsiella pneumoniae: Epidemiology, Hypervirulence-Associated Determinants, and Resistance Mechanisms.
The study highlights the emergence of carbapenem-resistant hypervirulent Klebsiella pneumoniae (hvKP) strains, particularly those harboring the blaKPC-2 and blaSHV-11 genes, which confer resistance to carbapenems and beta-lactams, respectively.
Successive Emergence of Ceftazidime-Avibactam Resistance through Distinct Genomic Adaptations in blaKPC-2-Harboring Klebsiella pneumoniae Sequence Type 307 Isolates.
The study identifies a 532G→T mutation in blaKPC-2 leading to D179Y substitution, which confers resistance to ceftazidime-avibactam in Klebsiella pneumoniae ST307 isolates. Additionally, genomic adaptations such as insertion sequences and gene disruptions contribute to resistance mechanisms.
Risk Factors for Carbapenem-resistant Klebsiella pneumoniae Infection and Mortality of Klebsiella pneumoniae Infection.
The study identifies the KPC gene as a factor in carbapenem resistance in Klebsiella pneumoniae, highlighting its role in resistance mechanisms and associations with increased mortality.
Evaluation of the Amplidiag CarbaR+VRE Kit for Accurate Detection of Carbapenemase-Producing Bacteria.
The Amplidiag CarbaR+VRE assay effectively detects various carbapenemase genes, including blaKPC, blaNDM, blaVIM, blaIMP, blaOXA-48, blaOXA-23, blaOXA-58, and blaOXA-51, in different bacterial species.
Translating genomics into practice for real-time surveillance and response to carbapenemase-producing Enterobacteriaceae: evidence from a complex multi-institutional KPC outbreak.
The study characterizes the blaKPC gene as a key factor in carbapenem resistance among KPC-producing Enterobacteriaceae, highlighting its role in the outbreak.
Translating genomics into practice for real-time surveillance and response to carbapenemase-producing Enterobacteriaceae: evidence from a complex multi-institutional KPC outbreak.
The study characterizes the blaKPC gene as a key factor in carbapenem resistance among KPC-producing Enterobacteriaceae, highlighting its role in the outbreak.
Characteristics of diarrheagenic Escherichia coli among children under 5 years of age with acute diarrhea: a hospital based study.
The study identified blaNDM-1 and blaKPC-2 as the main carbapenemase genes in carbapenem-resistant DEC strains. blaTEM-1 and blaCTX-M-55 were the major genetic determinants associated with high levels of cephalosporin resistance.
Detection of 16S rRNA and KPC Genes from Complex Matrix Utilizing a Molecular Inversion Probe Assay for Next-Generation Sequencing.
The study demonstrates the use of a molecular inversion probe (MIP) assay for the detection of 16S rRNA and KPC genes from complex matrices, showing high sensitivity and specificity for KPC gene detection.
Mechanism for carbapenem resistance of clinical Enterobacteriaceae isolates.
The study identifies KPC-2 carbapenemase as a primary mechanism of carbapenem resistance in clinical Enterobacteriaceae isolates, along with reduced expression of outer membrane porin proteins OmpK35, OmpK36, OmpC, and OmpF.
Klebsiella pneumoniae Carbapenemase Producers in South Korea between 2013 and 2015.
The study identified KPC-2, KPC-3, and KPC-4 as the main subtypes of the bla KPC gene in KPC-producing Enterobacteriaceae in South Korea. These genes were associated with various transposon isotypes, contributing to the spread and resistance profiles of these bacteria.
Klebsiella pneumoniae Carbapenemase Producers in South Korea between 2013 and 2015.
The study identified KPC-2, KPC-3, and KPC-4 as the main subtypes of the bla KPC gene in KPC-producing Enterobacteriaceae in South Korea. These genes were associated with various transposon isotypes, contributing to the spread and resistance profiles of these bacteria.
Klebsiella pneumoniae Carbapenemase Producers in South Korea between 2013 and 2015.
The study identified KPC-2, KPC-3, and KPC-4 as the main subtypes of the bla KPC gene in KPC-producing Enterobacteriaceae in South Korea. These genes were associated with various transposon isotypes, contributing to the spread and resistance profiles of these bacteria.
Molecular Characterization of Multidrug Resistant Uropathogenic E. Coli Isolates from Jordanian Patients.
The study identified high rates of multidrug-resistant uropathogenic E. coli isolates in Jordan, with a significant proportion carrying blaCTX-M-15, blaCTX-M-I, and blaCTX-M-9 genes, as well as mutations in parC and gyrA contributing to fluoroquinolone resistance. One isolate was found to harbor the blaKPC-2 carbapenemase gene.
Characteristics of Carbapenemase-Producing Enterobacteriaceae in Wastewater Revealed by Genomic Analysis.
The study identified various carbapenemase-encoding genes, including bla GES-5, bla GES-6, bla GES-24, bla NDM-5, bla IMP-8, bla IMP-19, bla KPC-2, and bla VIM-1, in carbapenemase-producing Enterobacteriaceae (CPE) isolated from wastewater in Japan and Taiwan.
Comprehensive Molecular Characterization of Escherichia coli Isolates from Urine Samples of Hospitalized Patients in Rio de Janeiro, Brazil.
The study identified various AMR genes including blaCTX-M-15, blaCTX-M-8, blaCMY-2, blaKPC-2, blaOXA-1, and blaTEM-1B in E. coli isolates from hospitalized patients in Rio de Janeiro, Brazil. These genes were associated with resistance to multiple antibiotics, highlighting the presence of multidrug-resistant clones.
Pneumonia and Renal Replacement Therapy Are Risk Factors for Ceftazidime-Avibactam Treatment Failures and Resistance among Patients with Carbapenem-Resistant Enterobacteriaceae Infections.
The study identifies bla KPC-3 mutations as the mechanism of ceftazidime-avibactam resistance in K. pneumoniae, highlighting the role of specific genetic alterations in resistance development.
Comprehensive Evaluation of the MBT STAR-BL Module for Simultaneous Bacterial Identification and beta-lactamase-Mediated Resistance Detection in Gram-Negative Rods from Cultured Isolates and Positive Blood Cultures.
The study evaluated the MBT STAR-BL module for detecting beta-lactamase-mediated resistance in Gram-negative rods from cultured isolates and blood cultures. It identified several beta-lactamase genes, including blaTEM-1b, blaCTX-M-13, blaCTX-M-14, blaNDM-5, blaKPC-2, blaCMY-2, blaOXA-23, blaOXA-51, blaVIM-4, blaPOM-1, and blaL1, which confer resistance to various β-lactam antibiotics.
Performance of "RESIST-3 O.K.N. K-SeT" immunochromatographic assay for the detection of OXA-48 like, KPC, and NDM carbapenemases in Klebsiella pneumoniae in Turkey.
The study evaluated the performance of the RESIST-3 O.K.N. K-SeT immunochromatographic assay for detecting OXA-48-like, KPC, and NDM carbapenemases in Klebsiella pneumoniae isolates. The assay showed 100% concordance with PCR results for bla OXA-48, bla NDM, and bla KPC genes.
Molecular epidemiology and drug resistant mechanism in carbapenem-resistant Klebsiella pneumoniae isolated from pediatric patients in Shanghai, China.
The study identified various AMR genes in CR-KP isolates, including blaNDM-1, blaIMP, blaKPC-2, SHV, TEM, CTX-M, aac(6')-Ib-cr, qnrS1, qnrB4, oqxAB, and rmtB, which contribute to resistance against carbapenems, beta-lactams, aminoglycosides, and fluoroquinolones.
Modified Carba NP Test: Simple and rapid method to differentiate KPC‐ and MBL‐producing Klebsiella species.
The study evaluated the modified Carba NP test for differentiating KPC and MBL-producing Klebsiella species, demonstrating high sensitivity and specificity for detecting carbapenemase-producing strains.
Relebactam Is a Potent Inhibitor of the KPC-2 beta-lactamase and Restores Imipenem Susceptibility in KPC-Producing Enterobacteriaceae.
Relebactam effectively inhibits the KPC-2 beta-lactamase, restoring imipenem susceptibility in KPC-producing Enterobacteriaceae.
Strategic Approaches to Overcome Resistance against Gram-Negative Pathogens Using beta-lactamase Inhibitors and β-Lactam Enhancers: Activity of Three Novel Diazabicyclooctanes WCK 5153, Zidebactam (WCK 5107), and WCK 4234.
The study characterizes three novel diazabicyclooctanes (WCK 5153, zidebactam, and WCK 4234) as potent beta-lactamase inhibitors effective against various beta-lactamases, including KPC-2, OXA-23, OXA-24/40, OXA-48, ADC-7, and PDC-3. These compounds restore susceptibility of multidrug-resistant Gram-negative pathogens to β-lactam antibiotics.
Resistance to Carbapenems in Non-Typhoidal Salmonella enterica Serovars from Humans, Animals and Food.
The study identifies various carbapenemase genes, including blaKPC-2, blaIMP-4, blaNDM-1, blaNDM-5, blaVIM-2, and blaOXA-48, in non-typhoidal Salmonella enterica serovars from humans, animals, and food sources, highlighting the emergence of carbapenem resistance in these pathogens.
Carbapenemase-producing Enterobacteriaceae in Mexico: report of seven non-clonal cases in a pediatric hospital.
The study identifies seven non-clonal carbapenemase-producing Enterobacteriaceae isolates in a pediatric hospital in Mexico, including E. coli producing OXA-232, NDM-1, and KPC-2; K. pneumoniae producing KPC-2 and NDM-1; K. oxytoca producing OXA-48; and E. cloacae producing NDM-1.
Clinically Relevant Plasmid-Host Interactions Indicate that Transcriptional and Not Genomic Modifications Ameliorate Fitness Costs of Klebsiella pneumoniae Carbapenemase-Carrying Plasmids.
The study identified that pKpQIL-UK and pKpQIL-D2 plasmids affect gene expression in K. pneumoniae without causing genomic mutations. These plasmids influence resistance to carbapenems and other antibiotics through the expression of genes like blaKPC and blaTEM, as well as efflux pumps such as acrAB and oqxB.
Fatal case of donor-derived colistin-resistant carbapenemase-producing Klebsiella pneumoniae transmission in cardiac transplantation.
The study reports a fatal case of donor-derived transmission of colistin-resistant carbapenemase-producing Klebsiella pneumoniae (ColR KPC-Kp) in cardiac transplantation, highlighting the presence of the blaKPC resistance gene in both donor and recipient isolates.
Antibacterial Spectrum of a Tetrazole-Based Reversible Inhibitor of Serine beta-lactamases.
The study characterizes the antibacterial spectrum of a tetrazole-based reversible inhibitor of serine beta-lactamases, showing significant activity against various CTX-M subtypes and other serine beta-lactamases, including CTX-M-9, CTX-M-14, CTX-M-15, CTX-M-27, SHV-2, KPC-2, AmpC, OXA-48, and TEM-1.
Genomic and Geographic Context for the Evolution of High-Risk Carbapenem-Resistant Enterobacter cloacae Complex Clones ST171 and ST78.
The study identifies blaKPC-3, blaKPC-4, blaKPC-2, and blaNDM-1 as key carbapenem resistance genes in Enterobacter cloacae complex clones ST171 and ST78. Mutations in gyrA and parC contribute to fluoroquinolone resistance.
Carbapenem Nonsusceptible Klebsiella pneumoniae in Taiwan: Dissemination and Increasing Resistance of Carbapenemase Producers During 2012-2015.
Carbapenem Nonsusceptible Klebsiella pneumoniae in Taiwan: Dissemination and Increasing Resistance of Carbapenemase Producers During 2012-2015.
Carbapenem Nonsusceptible Klebsiella pneumoniae in Taiwan: Dissemination and Increasing Resistance of Carbapenemase Producers During 2012-2015.
Diversity of Carbapenemase-Producing Escherichia coli Isolates in France in 2012-2013.
The study identified various carbapenemase-producing Escherichia coli isolates in France, including OXA-48-like, NDM, VIM, and KPC carbapenemases. These isolates exhibited resistance to carbapenems, with OXA-48-like being the most prevalent.
Case Report of an Extensively Drug-Resistant Klebsiella pneumoniae Infection With Genomic Characterization of the Strain and Review of Similar Cases in the United States.
The study identifies multiple AMR genes and mutations in an XDR-KP strain, including blaKPC-3, blaOXA-9, blaTEM-1A, and various resistance mechanisms involving efflux pumps, porin deficiencies, and fluoroquinolone resistance mutations.
Whole genome sequence revealed the fine transmission map of carbapenem-resistant Klebsiella pneumonia isolates within a nosocomial outbreak.
The study identified the presence of blaKPC-2, blaSHV-1, and blaCTX-M-15 genes in all 26 CRKP isolates, which conferred resistance to multiple beta-lactam antibiotics. The isolates belonged to sequence type ST11 and exhibited a clonal outbreak pattern.
Clonal Background, Resistance Gene Profile, and Porin Gene Mutations Modulate In Vitro Susceptibility to Imipenem-Relebactam in Diverse Enterobacteriaceae.
The study found that the addition of relebactam (REL) reduced imipenem (IMI) minimum inhibitory concentrations (MICs) to the susceptible range for 88% of clinical carbapenem-resistant Enterobacteriaceae (CRE) isolates, highlighting the effectiveness of IMI-REL against CRE, especially those harboring bla KPC.
Within-a-Day Detection and Rapid Characterization of Carbapenemase by Use of a New Carbapenem Inactivation Method-Based Test, CIMplus.
The study evaluates the CIMplus test for rapid detection and characterization of carbapenemase-producing Enterobacteriaceae (CPE), demonstrating high sensitivity and specificity in identifying various carbapenemase types, including KPC, GES, NDM, VIM, IMP, OXA-48, and OXA-181.
Tailoring antimicrobials in febrile neutropenia: using faster diagnostic and communication tools to improve treatment in the era of extensively resistant pathogens.
The case report describes the successful treatment of a patient with colistin-resistant CRKP bacteremia using ceftazidime-avibactam, highlighting the utility of rapid diagnostic methods and real-time communication tools in guiding antimicrobial therapy.
Past and Present Perspectives on β-Lactamases
The paper discusses the evolution and classification of β-lactamases, highlighting their role in antibiotic resistance and the emergence of new variants such as TEM-1, SHV-1, OXA-1, and AmpC.
Bloodstream infections caused by Klebsiella pneumoniae: prevalence of bla(KPC), virulence factors and their impacts on clinical outcome.
The study identified bla(KPC) as a significant factor in carbapenem resistance among Klebsiella pneumoniae bloodstream infections, with a strong association with increased 14-day mortality.
Molecular Characterization of Carbapenem-Resistant Enterobacter cloacae in 11 Chinese Cities.
The study identified various carbapenemase genes, including blaNDM-1, blaIMP-4, blaKPC-2, and blaVIM-1, in carbapenem-resistant Enterobacter cloacae strains from 11 Chinese cities. These genes conferred resistance to multiple beta-lactam antibiotics.
Direct Detection of Carbapenem-Resistant Organisms from Environmental Samples Using the GeneXpert Molecular Diagnostic System.
The study evaluated the effectiveness of the Carba-R assay for detecting carbapenemase genes in environmental samples during a healthcare-associated outbreak. The Carba-R assay detected carbapenemase genes in several samples, including bla KPC, bla VIM, and bla OXA-48-type, demonstrating its utility in identifying carbapenem-resistant organisms.
Enterobacter cloacae harbouring bla(KPC-2) and qnrB-1 isolated from a cystic fibrosis patient: a case report.
The study identified the presence of bla(KPC-2) and qnrB-1 genes in an Enterobacter cloacae isolate from a cystic fibrosis patient, highlighting the potential for dissemination of carbapenem and quinolone resistance.
Molecular Characterization of Extended-Spectrum β-Lactamase-Producing Multidrug Resistant Escherichia coli From Swine in Northwest China.
The study identified multiple β-lactamase genes, including bla CTX-M, bla TEM, bla SHV, bla OXA-48, and bla KPC-2, as well as PMQR genes such as qnrS and aac(6')-Ib-cr, in multidrug-resistant E. coli isolates from swine in Northwest China.
Molecular characterization and epidemiology of carbapenem non-susceptible Enterobacteriaceae isolated from the Eastern region of Heilongjiang Province, China.
The study identified bla KPC-2, bla NDM, bla SHV, bla TEM, and bla CTX-M-15 as the main drug resistance genes in carbapenem non-susceptible Enterobacteriaceae (CNSE) isolates from the Eastern region of Heilongjiang Province, China. The primary resistance mechanism was the production of carbapenemases and extended-spectrum beta-lactamases.
Defining the architecture of KPC-2 Carbapenemase: identifying allosteric networks to fight antibiotics resistance.
The study identifies the alpha-network in KPC-2 as crucial for structural integrity and resistance to beta-lactam antibiotics. Disruption of this network through mutations leads to restored susceptibility.
Phenotypic screening and molecular characterization of carbapenemase-producing Gram-negative bacilli recovered from febrile neutropenic pediatric cancer patients in Egypt.
The study identified blaOXA-48, blaNDM, blaVIM, and blaKPC as the主要 carbapenemase genes in carbapenem-resistant Gram-negative bacilli from febrile neutropenic pediatric cancer patients in Egypt.
Related carbapenemase-producing Klebsiella isolates detected in both a hospital and associated aquatic environment in Sweden.
The study identified carbapenemase-producing Klebsiella isolates in both hospital and aquatic environments in Sweden, highlighting the presence of blaVIM-1, blaIMP-29, blaNDM-1, blaKPC-3, and blaOXA-48 genes in various Klebsiella species.
Detection of high-risk carbapenem-resistant Klebsiella pneumoniae and Enterobacter cloacae isolates using volatile molecular profiles.
The study identifies blaKPC as a gene associated with carbapenem resistance in Klebsiella pneumoniae, Enterobacter cloacae, and Escherichia coli through volatile metabolic profiling.
Prosthetic Joint Infection from Carbapenemase-Resistant Klebsiella pneumoniae Successfully Treated with Ceftazidime-Avibactam.
The case report describes the successful treatment of a prosthetic joint infection caused by carbapenemase-resistant Klebsiella pneumoniae (CRKP) with ceftazidime-avibactam. The isolate was confirmed to produce KPC carbapenemase through real-time PCR and phenotypic assays.
A Large, Refractory Nosocomial Outbreak of Klebsiella pneumoniae Carbapenemase-Producing Escherichia coli Demonstrates Carbapenemase Gene Outbreaks Involving Sink Sites Require Novel Approaches to Infection Control.
The study identifies the blaKPC gene as a key factor in a large outbreak of carbapenem-resistant Escherichia coli, highlighting the role of sink sites in the persistence and spread of carbapenemase-producing organisms.
Comparative genomics of five different resistance plasmids coexisting in a clinical multi-drug resistant Citrobacter freundii isolate.
The study identifies five resistance plasmids in a clinical Citrobacter freundii isolate, each carrying multiple AMR genes, including bla NDM-1, bla IMP-4, qnrS1, bla KPC-2, catB3, and mph (A), contributing to multidrug resistance.
Analyses of a Ceftazidime-Avibactam-Resistant Citrobacter freundii Isolate Carrying bla(KPC-2) Reveals a Heterogenous Population and Reversible Genotype.
The study identifies mutations in the blaKPC-2 gene of a Citrobacter freundii isolate that confer resistance to ceftazidime-avibactam. These mutations, including D176Y, R164S, P147L, and D179Y, are located in the omega-loop region of the gene and were experimentally validated through cloning and susceptibility testing.
Klebsiella grimontii, a New Species Acquired Carbapenem Resistance.
The study reports the first carbapenem-resistant strain of Klebsiella grimontii, which harbors the blaKPC-2 gene on a plasmid, leading to resistance against imipenem and meropenem.
A Fatal Bacteremia Caused by Hypermucousviscous KPC-2 Producing Extensively Drug-Resistant K64-ST11 Klebsiella pneumoniae in Brazil.
The study reports a fatal case of bacteremia caused by a hypermucoviscous KPC-2 producing extensively drug-resistant K64-ST11 Klebsiella pneumoniae. The isolate harbored multiple AMR genes including blaKPC-2, blaSHV-11, qnrS1, oqxA, oqxB, sul1, sul2, dfrA1, tetA, tetD, and fosA, contributing to its extensive drug resistance.
Establishment of a dual-wavelength spectrophotometric method for analysing and detecting carbapenemase-producing Enterobacteriaceae.
The study established a dual-wavelength spectrophotometric method for detecting carbapenemase-producing Enterobacteriaceae (CPE) by analyzing imipenem hydrolysis. The method effectively identified CPE strains carrying blaIMP, blaKPC, blaNDM, blaOXA, and blaVIM genes.
Draft genome sequence data of a tigecycline-resistant Enterobacter cloacae ST93 clinical strain isolated from bloodstream infection.
The study reports the draft genome sequence of a tigecycline-resistant Enterobacter cloacae ST93 clinical isolate, TREC1, which harbors multiple antimicrobial resistance genes, including those encoding resistance to beta-lactams, aminoglycosides, fluoroquinolones, fosfomycin, macrolides, lincosamides, streptogramin B, phenicols, sulfonamides, trimethoprim, and tetracyclines. The isolate is resistant to all antibiotics tested except colistin.
Applying Rapid Whole-Genome Sequencing To Predict Phenotypic Antimicrobial Susceptibility Testing Results among Carbapenem-Resistant Klebsiella pneumoniae Clinical Isolates.
The study identified various AMR genes and mutations in carbapenem-resistant Klebsiella pneumoniae isolates, including bla KPC-2, bla KPC-3, bla NDM-1, bla OXA-48, ampC, qnrB, qnrS, aac(6')-Ib-cr, armA, rmtB, tet(A), tet(B), tet(D), tet(G), sul1, sul2, sul3, dfrA1, dfrA12, dfrA14, dfrA25, dfrA26, dfrA30, oqxA, oqxB, and mgrB, as well as mutations in ompK35, ompK36, gyrA, parC, phoP, phoQ, pmrA, and pmrB, which contribute to resistance against multiple antibiotics.
Applying Rapid Whole-Genome Sequencing To Predict Phenotypic Antimicrobial Susceptibility Testing Results among Carbapenem-Resistant Klebsiella pneumoniae Clinical Isolates.
The study identified various AMR genes and mutations in carbapenem-resistant Klebsiella pneumoniae isolates, including bla KPC-2, bla KPC-3, bla NDM-1, bla OXA-48, ampC, qnrB, qnrS, aac(6')-Ib-cr, armA, rmtB, tet(A), tet(B), tet(D), tet(G), sul1, sul2, sul3, dfrA1, dfrA12, dfrA14, dfrA25, dfrA26, dfrA30, oqxA, oqxB, and mgrB, as well as mutations in ompK35, ompK36, gyrA, parC, phoP, phoQ, pmrA, and pmrB, which contribute to resistance against multiple antibiotics.
Prospective evaluation of a screening algorithm for carbapenemase-producing Enterobacteriaceae.
The study evaluated a screening algorithm for carbapenemase-producing Enterobacteriaceae (CPE) and identified several carbapenemase genes, including bla KPC, bla NDM, bla VIM, bla IMP, bla OXA-48, and bla OXA-181, which confer resistance to carbapenems.
First Description of Colistin and Tigecycline-Resistant Acinetobacter baumannii Producing KPC-3 Carbapenemase in Portugal.
The study describes the first identification of a colistin and tigecycline-resistant Acinetobacter baumannii isolate producing KPC-3 carbapenemase in Portugal, highlighting the emergence of multidrug-resistant strains with significant implications for treatment options.
Rapid detection of carbapenemases directly from positive blood cultures by the β-CARBA test.
The study presents a new method for the rapid detection of carbapenemases directly from positive blood cultures using the β-CARBA test, achieving 100% sensitivity and 94.3% specificity for various carbapenemase types including OXA-48-like, NDM, KPC, VIM, and GIM.
Rapid detection of resistance to carbapenems and cephalosporins in Enterobacteriaceae using liquid chromatography tandem mass spectrometry.
The study developed a rapid resistance testing (RRT) protocol using liquid chromatography tandem mass spectrometry (LC-MS/MS) to detect hydrolysis of beta-lactam antibiotics, particularly imipenem, in carbapenemase-producing Enterobacteriaceae (CPE). The RRT method showed high sensitivity and specificity for CPE detection, with 100% accuracy for imipenem hydrolysis cutoffs between 50% and 80%.
Identification and Characterization of NDM-1-producing Hypervirulent (Hypermucoviscous) Klebsiella pneumoniae in China.
The study identifies NDM-1 and KPC-2 producing hypervirulent Klebsiella pneumoniae isolates, highlighting their resistance to carbapenems and association with hospital outbreaks.
Evaluation of Modified Hodge Test as a Non-molecular Assay for Accurate Detection of KPC-producing Klebsiella pneumoniae.
The study evaluated the Modified Hodge Test (MHT) for detecting KPC-producing Klebsiella pneumoniae and identified that only five out of 54 MHT-positive isolates carried the blaKPC gene, highlighting limitations in MHT specificity for KPC detection.
The resistomes of six carbapenem-resistant pathogens - a critical genotype-phenotype analysis.
The study characterized the resistomes of six carbapenem-resistant pathogens, identifying various carbapenemase genes such as bla KPC-2, bla OXA-48, bla OXA-72, bla NDM-1, bla NDM-7, and bla VIM-1, along with other resistance genes like aac(6')-Ib-cr, aph(3")-Ib, aph(6)-Id, tet(B), erm(B), mph(A), sul1, sul2, dfrA17, dfrA14, bla CTX-M-15, bla CMY-6, bla OXA-1, bla SHV-200, bla OXA-10, and bla NDM-7.
Characterization of Gene Families Encoding Beta-Lactamases of Gram-Negative Rods Isolated from Ready-to-Eat Vegetables in Mexico City.
The study identified various beta-lactamase genes, including blaBIL, blaSHV, blaCTX, blaDHA, blaVIM, blaOXA, blaIMP, blaKPC, and blaTEM, which confer resistance to beta-lactam antibiotics in Enterobacteriaceae isolated from ready-to-eat vegetables in Mexico City.
In vitro and in vivo bactericidal activity of ceftazidime-avibactam against Carbapenemase-producing Klebsiella pneumoniae.
Ceftazidime-avibactam showed significant bactericidal activity against KPC-2 and OXA-232 carbapenemase-producing Klebsiella pneumoniae. Combining ceftazidime-avibactam with aztreonam resulted in synergistic effects against NDM carbapenemase-producing strains.
Diversity of resistance mechanisms in carbapenem-resistant Enterobacteriaceae at a health care system in Northern California, from 2013 to 2016.
The study identified various carbapenemase genes, including bla OXA-48 like, bla KPC, bla NDM, bla SME, bla IMP, and bla VIM, contributing to carbapenem resistance in Enterobacteriaceae isolates. These genes were found in different bacterial species, and their presence influenced the susceptibility profiles of the isolates to newer β-lactam/β-lactamase inhibitors.
In silico identification and experimental validation of hits active against KPC-2 beta-lactamase.
The study identified and experimentally validated compounds that inhibit the KPC-2 beta-lactamase, a major cause of carbapenem resistance in Klebsiella pneumoniae. Compound 11a showed significant inhibitory activity against KPC-2, reducing the MIC of meropenem by four-fold in clinical strains.
Prevalence of blaKPC and its occurrence with other beta-lactamases in Klebsiella pneumoniae.
The study detected the blaKPC gene in one isolate of Klebsiella pneumoniae, which was co-produced with SHV-12 and CTX-M-15. PCR was confirmed as the gold standard for detecting KPC.
Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE).
The study identified the novel blaKPC-21 gene, along with blaGES-5 and blaGES-6, in carbapenem-resistant Enterobacteriaceae isolates in Portugal, highlighting the emergence of new carbapenemase variants and their association with high-risk clones.
Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE).
Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE).
Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE).
A Full MALDI-Based Approach to Detect Plasmid-Encoded KPC-Producing Klebsiella pneumoniae.
The study presents a full MALDI-based approach to detect plasmid-encoded KPC-producing Klebsiella pneumoniae strains through automated detection of a KPC-specific peak and confirmation of carbapenemase activity.
Transcriptional Landscape of a bla (KPC-2) Plasmid and Response to Imipenem Exposure in Escherichia coli TOP10.
The study identifies the expression of AMR genes bla KPC−2, bla TEM−1, aph(3′)-I, and mepM in E. coli TOP10 harboring the pBIC1a plasmid, highlighting their role in resistance to carbapenems, beta-lactams, aminoglycosides, and beta-lactams respectively.
Population Structure, Antibiotic Resistance, and Uropathogenicity of Klebsiella variicola.
The study identifies several antibiotic resistance genes in Klebsiella variicola, including blaLEN, oqxAB, blaKPC-2, blaNDM-1, blaNDM-9, blaOXA-48, aac(6')-Ib, aadA16, sul1, sul2, qnrB6, arr-3, and floR. These genes confer resistance to various antibiotics such as ampicillin, ciprofloxacin, meropenem, gentamicin, kanamycin, sulfamethoxazole, rifampin, and chloramphenicol.
Meropenem-Vaborbactam as Salvage Therapy for Ceftazidime-Avibactam-Resistant Klebsiella pneumoniae Bacteremia and Abscess in a Liver Transplant Recipient.
The study identified a D179Y mutation in the blaKPC-2 gene, which conferred resistance to ceftazidime-avibactam while reducing meropenem hydrolytic activity in a KPC-producing Klebsiella pneumoniae isolate.
Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae ST258 isolated from a Japanese patient without a history of foreign travel - a new public health concern in Japan: a case report.
The study identifies a KPC-2-producing Klebsiella pneumoniae ST258 isolate from a Japanese patient without a history of foreign travel, highlighting the emergence of carbapenem-resistant strains in Japan.
Effects of KPC Variant and Porin Genotype on the In Vitro Activity of Meropenem-Vaborbactam against Carbapenem-Resistant Enterobacteriaceae.
The study characterized the effects of KPC variants and porin mutations on the in vitro activity of meropenem-vaborbactam against carbapenem-resistant Enterobacteriaceae. It showed that meropenem-vaborbactam was effective against KPC-producing isolates, including those resistant to ceftazidime-avibactam, and highlighted the impact of ompK36 mutations on meropenem-vaborbactam susceptibility.
Results from the China Antimicrobial Surveillance Network (CHINET) in 2017 of the In Vitro Activities of Ceftazidime-Avibactam and Ceftolozane-Tazobactam against Clinical Isolates of Enterobacteriaceae and Pseudomonas aeruginosa.
The study identified bla KPC and bla NDM carbapenemase genes in carbapenem-resistant Enterobacteriaceae and Pseudomonas aeruginosa isolates, highlighting their prevalence and impact on antimicrobial resistance.
Simultaneous Single-Channel Multiplexing and Quantification of Carbapenem-Resistant Genes Using Multidimensional Standard Curves.
The study presents a novel method for simultaneous single-channel multiplexing and enhanced quantification of four carbapenem-resistant genes: blaOXA-48, blaNDM, blaVIM, and blaKPC. These genes were experimentally validated using synthetic DNA and bacterial isolates.
Improved blood culture workflow for faster identification of KPC-producing Enterobacterales.
The study evaluated the detection of KPC-producing Enterobacterales using bacterial pellets from blood cultures, identifying blaKPC-2 as the predominant carbapenemase gene.
Diarrheal bacterial pathogens and multi-resistant enterobacteria in the Choqueyapu River in La Paz, Bolivia.
The study identified multidrug-resistant Enterobacteriaceae in the Choqueyapu River, including E. coli and Enterobacter cloacae carrying bla CTX-M, bla KPC, bla NDM, bla VIM, and bla OXA-48 genes, highlighting the environmental spread of antibiotic resistance.
Reconstruction of the Genomes of Drug-Resistant Pathogens for Outbreak Investigation through Metagenomic Sequencing.
The study successfully reconstructed the genomes of drug-resistant pathogens, including KPC-producing K. pneumoniae and vanB VRE, demonstrating the utility of metagenomic sequencing in outbreak investigations.
Identification, Characterization, and Formulation of a Novel Carbapenemase Intended to Prevent Antibiotic-Mediated Gut Dysbiosis.
The study identified and characterized P2A, NDM, and KPC carbapenemases, which degrade various beta-lactam antibiotics, with P2A being the most stable in human intestinal chyme and suitable for clinical application.
Molecular Characterization of Carbapenem Resistant Klebsiella pneumoniae and Klebsiella quasipneumoniae Isolated from Lebanon.
The study identified several carbapenemase genes, including bla OXA-48, bla NDM-1, bla NDM-7, and bla CTX-M-15, as well as the chromosomally-encoded beta-lactamase bla OKP-B-3, in carbapenem-resistant Klebsiella pneumoniae and Klebsiella quasipneumoniae isolates from Lebanon.
Genetic Diversity of Carbapenem-Resistant Enterobacteriaceae (CRE) Clinical Isolates From a Tertiary Hospital in Eastern China.
The study identified multiple carbapenemase genes (blaKPC-2, blaKPC-3, blaNDM-1, blaNDM-5, blaIMP-4, and blaVIM-1) and porin gene mutations (ompK35, ompK36, and ompF) contributing to carbapenem resistance in CRE isolates from a Chinese hospital.
Genetic Diversity of Carbapenem-Resistant Enterobacteriaceae (CRE) Clinical Isolates From a Tertiary Hospital in Eastern China.
The study identified multiple carbapenemase genes (blaKPC-2, blaKPC-3, blaNDM-1, blaNDM-5, blaIMP-4, and blaVIM-1) and porin gene mutations (ompK35, ompK36, and ompF) contributing to carbapenem resistance in CRE isolates from a Chinese hospital.
High Prevalence of Multidrug-Resistant Klebsiella pneumoniae Harboring Several Virulence and beta-lactamase Encoding Genes in a Brazilian Intensive Care Unit.
The study identified a high prevalence of multidrug-resistant Klebsiella pneumoniae isolates carrying various beta-lactamase genes (bla KPC, bla TEM, bla SHV, bla OXA-1, and bla CTX-M-1) and efflux pump genes (AcrAB, tol C, mdt K) along with outer membrane porin genes (Omp K35 and Omp K36).
Ceftazidime/avibactam resistance associated with L169P mutation in the omega loop of KPC-2.
Ceftazidime/avibactam resistance associated with L169P mutation in the omega loop of KPC-2.
Ceftazidime/avibactam resistance associated with L169P mutation in the omega loop of KPC-2.
Synergistic Effect of Ceftazidime-Avibactam with Meropenem against Panresistant, Carbapenemase-Harboring Acinetobacter baumannii and Serratia marcescens Investigated Using Time-Kill and Disk Approximation Assays.
The study investigated the synergistic effects of ceftazidime-avibactam with meropenem against panresistant, carbapenemase-harboring Acinetobacter baumannii and Serratia marcescens. It found that ceftazidime-avibactam was effective against S. marcescens isolates carrying bla KPC-2, while synergy with meropenem was observed in some A. baumannii isolates co-harboring bla OXA-23 and bla OXA-117.
Genome plasticity favours double chromosomal Tn4401b-bla(KPC-2) transposon insertion in the Pseudomonas aeruginosa ST235 clone.
The study identifies the presence of two complete and intact copies of bla KPC-2 -harbouring Tn 4401b transposons inserted into the chromosome of Pseudomonas aeruginosa ST235 isolate 24Pae112 within a new genomic island called PAGI-17.
High Burden of Extended-Spectrum beta-lactamase-Producing Escherichia coli and Klebsiella pneumoniae Bacteremia in Older Adults: A Seven-Year Study in Two Rural Thai Provinces.
The study identified the presence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae in two rural Thai provinces, highlighting the increasing prevalence of these resistant strains and their association with higher mortality rates and reduced susceptibility to various antibiotics.
Dynamic Colonization of Klebsiella pneumoniae Isolates in Gastrointestinal Tract of Intensive Care Patients.
The study identifies blaKPC-2 as the predominant carbapenem resistance gene in CRKP isolates from ICU patients, highlighting its role in resistance and its ability to be incorporated into various plasmids for horizontal transfer.
Infections Caused by Carbapenemase-Producing Klebsiella pneumoniae: Microbiological Characteristics and Risk Factors.
All CPKP strains examined were positive for bla kpc-2, which confers resistance to carbapenems.
Comparative Analysis of blaKPC Expression in Tn4401 Transposons and the Tn3-Tn4401 Chimera
The study identifies a novel promoter PX in the Tn3-Tn4401 chimera that contributes to blaKPC expression and carbapenem resistance, highlighting differences in resistance levels between various genetic structures carrying blaKPC.
Plasmid-Mediated Transmission of KPC-2 Carbapenemase in Enterobacteriaceae in Critically Ill Patients.
The study identifies the plasmid-mediated transmission of the KPC-2 carbapenemase among Enterobacteriaceae, highlighting the role of IncN plasmids in spreading carbapenem resistance.
A One Health Study of the Genetic Relatedness of Klebsiella pneumoniae and Their Mobile Elements in the East of England.
Interpretable genotype-to-phenotype classifiers with performance guarantees.
The study presents rule-based classifiers that accurately predict antimicrobial resistance using genome sequences, identifying known resistance mechanisms such as mutations in rrs for kanamycin resistance and genes like blaKPC-2, bleMBL, and blaNDM-1 for meropenem resistance.
Genomic characterization of a KPC-23-producing Klebsiella pneumoniae ST258 clinical isolate resistant to ceftazidime-avibactam.
The study identified blaKPC-23 as the primary carbapenemase gene responsible for ceftazidime-avibactam resistance in a KPC-23-producing Klebsiella pneumoniae ST258 clinical isolate. The gene was shown to confer high-level resistance to carbapenems and cephalosporins.
Genomic characterization of a KPC-23-producing Klebsiella pneumoniae ST258 clinical isolate resistant to ceftazidime-avibactam.
The study identified blaKPC-23 as the primary carbapenemase gene responsible for ceftazidime-avibactam resistance in a KPC-23-producing Klebsiella pneumoniae ST258 clinical isolate. The gene was shown to confer high-level resistance to carbapenems and cephalosporins.
Genomic characterization of a KPC-23-producing Klebsiella pneumoniae ST258 clinical isolate resistant to ceftazidime-avibactam.
The study identified blaKPC-23 as the primary carbapenemase gene responsible for ceftazidime-avibactam resistance in a KPC-23-producing Klebsiella pneumoniae ST258 clinical isolate. The gene was shown to confer high-level resistance to carbapenems and cephalosporins.
Genomic characterization of a KPC-23-producing Klebsiella pneumoniae ST258 clinical isolate resistant to ceftazidime-avibactam.
Genomic characterization of a KPC-23-producing Klebsiella pneumoniae ST258 clinical isolate resistant to ceftazidime-avibactam.
Characterization of Carbapenemase-Producing Klebsiella oxytoca in Spain, 2016-2017.
The study characterized carbapenemase-producing Klebsiella oxytoca isolates in Spain, identifying various carbapenemase genes such as bla OXA-48, bla VIM-1, bla KPC-2, bla KPC-3, and bla NDM-1, along with the bla OXY-2 beta-lactamase gene.
Characterization of Carbapenemase-Producing Klebsiella oxytoca in Spain, 2016-2017.
The study characterized carbapenemase-producing Klebsiella oxytoca isolates in Spain, identifying various carbapenemase genes such as bla OXA-48, bla VIM-1, bla KPC-2, bla KPC-3, and bla NDM-1, along with the bla OXY-2 beta-lactamase gene.
Susceptibility rates of clinically important bacteria collected from intensive care units against colistin, carbapenems, and other comparative agents: results from Surveillance of Multicenter Antimicrobial Resistance in Taiwan (SMART).
The study identified bla KPC and bla OXA48 carbapenemase genes in carbapenem-insusceptible Klebsiella pneumoniae isolates and mcr-1 colistin resistance gene in Enterobacter cloacae isolates.
A Phage-Like Plasmid Carrying bla (KPC-2) Gene in Carbapenem-Resistant Pseudomonas aeruginosa.
The study identifies a phage-like plasmid carrying the blaKPC-2 gene in a carbapenem-resistant Pseudomonas aeruginosa strain, highlighting the role of mobile genetic elements in the dissemination of antimicrobial resistance.
First clinical case of KPC-3-producing Klebsiella michiganensis in Europe.
The study reports the first clinical case of a KPC-3-producing Klebsiella michiganensis isolate in Europe, highlighting the emergence of this multidrug-resistant pathogen and the significance of molecular diagnostics in identifying novel resistance mechanisms.
Epidemiology and Mechanisms of Resistance of Extensively Drug Resistant Gram-Negative Bacteria.
The paper reviews the mechanisms of resistance in extensively drug-resistant (XDR) gram-negative bacteria, focusing on carbapenem resistance mechanisms such as carbapenemases (KPC, NDM, VIM, OXA-48), efflux pumps, porin mutations, and colistin resistance (mcr-1).
Hospital effluent: A reservoir for carbapenemase-producing Enterobacterales?
The study identified carbapenemase-producing Enterobacterales (CPE) in hospital effluent and municipal wastewater, with blaOXA-48, blaKPC, blaVIM, and blaIMP detected in hospital and post-hospital wastewater samples. blaNDM was found in pre-hospital wastewater.
Antibiotic Resistance in Enterobacteriaceae from Surface Waters in Urban Brazil Highlights the Risks of Poor Sanitation.
The study identified several AMR genes in Enterobacteriaceae from surface waters in urban Brazil, including bla OXA-48, bla KPC, bla VIM-2, qnrS, and aac(6')-lb-cr, highlighting the risks of poor sanitation.
Diversity, Virulence, and Antimicrobial Resistance in Isolates From the Newly Emerging Klebsiella pneumoniae ST101 Lineage.
The study characterizes the antimicrobial resistance genes and mutations in the emerging Klebsiella pneumoniae ST101 lineage, highlighting the presence of multiple resistance mechanisms including carbapenemases, extended-spectrum beta-lactamases, and various other resistance genes.
Identification of a Carbapenemase-Producing Hypervirulent Klebsiella pneumoniae Isolate in the United States.
The study identifies a carbapenemase-producing hypervirulent Klebsiella pneumoniae isolate (CP-hvKP) in the United States, which carries the blaKPC-2 gene on a plasmid, along with other resistance genes such as blaSHV-36, fosA, oqxA, and oqxB. The isolate also possesses hypervirulence factors, highlighting the dual threat of antimicrobial resistance and virulence.
Genotypic antimicrobial resistance assays for use on E. coli isolates and stool specimens.
The study developed and validated 85 PCR assays to detect 79 AMR genes and mutations associated with resistance across 10 antimicrobial classes, focusing on E. coli. The assays showed high concordance with sequencing and phenotypic susceptibility testing, demonstrating their potential for AMR surveillance in E. coli isolates and direct stool specimens.
Identification of L169P mutation in the omega loop of KPC-3 after a short course of ceftazidime/avibactam.
The L169P mutation in the omega loop of KPC-3 was identified as a cause of ceftazidime/avibactam resistance in a Klebsiella pneumoniae isolate.
Emergence of ceftazidime-avibactam-resistant Klebsiella pneumoniae during treatment, Finland, December 2018.
The study identifies a novel 15 amino acid insertion in the KPC-2 protein leading to ceftazidime-avibactam resistance in a KPC-2-producing Klebsiella pneumoniae ST39 strain isolated in Finland.
Emergence of ceftazidime-avibactam-resistant Klebsiella pneumoniae during treatment, Finland, December 2018.
Emergence of ceftazidime-avibactam-resistant Klebsiella pneumoniae during treatment, Finland, December 2018.
Potential KPC-2 carbapenemase reservoir of environmental Aeromonas hydrophila and Aeromonas caviae isolates from the effluent of an urban wastewater treatment plant in Japan.
The study identifies blaKPC-2 as a carbapenemase gene in Aeromonas hydrophila and Aeromonas caviae isolates from a wastewater treatment plant effluent in Japan, highlighting their potential as environmental reservoirs for carbapenem-resistant genes.
Direct use of eazyplex(®) SuperBug CRE assay from positive blood cultures in conjunction with inpatient infectious disease consulting for timely appropriate antimicrobial therapy in Escherichia coli and Klebsiella pneumoniae bloodstream infections.
The study highlights the effectiveness of the eazyplex® SuperBug CRE assay in detecting bla CTX-M, bla KPC, and bla VIM genes in Escherichia coli and Klebsiella pneumoniae bloodstream infections, enabling timely and appropriate antimicrobial therapy.
Infection caused by Klebsiella pneumoniae ST11 in a patient after craniectomy.
The study identifies blaSHV-11, blaTEM-1, blaCTX-M-15, and blaKPC-3 genes in multidrug-resistant K. pneumoniae ST11 isolates from a neurosurgical patient, highlighting their role in resistance to beta-lactam and carbapenem antibiotics.
Unravelling ceftazidime/avibactam resistance of KPC-28, a KPC-2 variant lacking carbapenemase activity.
Unravelling ceftazidime/avibactam resistance of KPC-28, a KPC-2 variant lacking carbapenemase activity.
Unravelling ceftazidime/avibactam resistance of KPC-28, a KPC-2 variant lacking carbapenemase activity.
Unravelling ceftazidime/avibactam resistance of KPC-28, a KPC-2 variant lacking carbapenemase activity.
Unravelling ceftazidime/avibactam resistance of KPC-28, a KPC-2 variant lacking carbapenemase activity.
Unravelling ceftazidime/avibactam resistance of KPC-28, a KPC-2 variant lacking carbapenemase activity.
The Likelihood of Developing a Carbapenem-Resistant Enterobacteriaceae Infection during a Hospital Stay.
The study identifies carbapenemase-producing CRE strains carrying bla KPC, bla NDM, and bla OXA-48 genes, highlighting the association between carbapenemase production and increased risk of CRE infections.
Genomic characterization of Escherichia coli LCT-EC001, an extremely multidrug-resistant strain with an amazing number of resistance genes.
The study identifies 68 antibiotic resistance determinants in E. coli LCT-EC001, including 8 beta-lactamase genes (blaTEM-1, blaCTX-M-14, blaKPC-2), 31 multidrug efflux system genes, 6 outer membrane transport system genes, 4 aminoglycoside-modifying enzyme genes, 10 two-component regulatory system genes, and 9 other enzyme or transcriptional regulator genes.
Active Surveillance of Carbapenemase-Producing Organisms (CPO) Colonization With Xpert Carba-R Assay Plus Positive Patient Isolation Proves to Be Effective in CPO Containment.
The study identified KPC, NDM, and IMP carbapenemase genes in CPO-colonized patients using the Xpert Carba-R assay, demonstrating its effectiveness in detecting carbapenemase-producing organisms and aiding in infection control through patient isolation.
Evaluation of the Synergy of Ceftazidime-Avibactam in Combination with Meropenem, Amikacin, Aztreonam, Colistin, or Fosfomycin against Well-Characterized Multidrug-Resistant Klebsiella pneumoniae and Pseudomonas aeruginosa.
The study evaluated the synergy of ceftazidime-avibactam (CZA) in combination with other antibiotics against multidrug-resistant Klebsiella pneumoniae and Pseudomonas aeruginosa. Several AMR genes, including blaKPC-2, blaKPC-3, blaCTX-M-15, and others, were identified as conferring resistance to CZA and other antibiotics.
Evaluation of the Synergy of Ceftazidime-Avibactam in Combination with Meropenem, Amikacin, Aztreonam, Colistin, or Fosfomycin against Well-Characterized Multidrug-Resistant Klebsiella pneumoniae and Pseudomonas aeruginosa.
The study evaluated the synergy of ceftazidime-avibactam (CZA) in combination with other antibiotics against multidrug-resistant Klebsiella pneumoniae and Pseudomonas aeruginosa. Several AMR genes, including blaKPC-2, blaKPC-3, blaCTX-M-15, and others, were identified as conferring resistance to CZA and other antibiotics.
Phenotypic and genotypic characterization of multi-drug-resistant Escherichia coli isolates harboring bla(CTX-M) group extended-spectrum β-lactamases recovered from pediatric patients in Shenzhen, southern China.
The study identified various bla CTX-M subtypes, including bla CTX-M-15, bla CTX-M-14, bla CTX-M-90, and others, as well as bla TEM, bla SHV, bla GES, bla CARB, bla NDM-1, bla KPC, and mcr-1, which contribute to multidrug resistance in E. coli isolates from pediatric patients in Shenzhen, China.
Laboratory and Clinical Evaluation of DNA Microarray for the Detection of Carbapenemase Genes in Gram-Negative Bacteria from Hospitalized Patients.
The study developed a DNA microarray for the detection of eight carbapenemase genes (blaKPC, blaNDM-1, blaOXA-23, blaOXA-48, blaOXA-51, blaIMP, blaVIM, and blaDIM) in Gram-negative bacteria, demonstrating high specificity and sensitivity.
Establishing Antimicrobial Resistance Surveillance & Research Network in India: Journey so far.
The study characterizes various AMR genes and mutations in different bacterial pathogens, highlighting the prevalence of resistance to multiple antibiotics, including beta-lactams, fluoroquinolones, and aminoglycosides. Key findings include the widespread presence of bla OXA-48, bla TEM, and qnrS1 in Enterobacteriaceae, and mecA, mupA, cfr, and ermC in Staphylococcus aureus.
Carbapenemase-producing Enterobacteriaceae and Aeromonas spp. present in wastewater treatment plant effluent and nearby surface waters in the US.
The study identifies various carbapenemase-producing bacteria, including Enterobacteriaceae and Aeromonas spp., in wastewater treatment plant effluent and nearby surface waters in the US. Key findings include the detection of bla KPC-2, bla KPC-3, bla NDM-1, bla NDM-5, bla NDM-7, bla GES-5, and bla VIM-1 genes in several bacterial species, highlighting the presence of clinically relevant carbapenem-resistant genotypes in the environment.
Carbapenemase-producing Enterobacteriaceae and Aeromonas spp. present in wastewater treatment plant effluent and nearby surface waters in the US.
The study identifies various carbapenemase-producing bacteria, including Enterobacteriaceae and Aeromonas spp., in wastewater treatment plant effluent and nearby surface waters in the US. Key findings include the detection of bla KPC-2, bla KPC-3, bla NDM-1, bla NDM-5, bla NDM-7, bla GES-5, and bla VIM-1 genes in several bacterial species, highlighting the presence of clinically relevant carbapenem-resistant genotypes in the environment.
Performance Evaluation of the MBT STAR(®)-Carba IVD Assay for the Detection of Carbapenemases With MALDI-TOF MS.
The MBT STAR®-Carba IVD assay effectively detects carbapenemase-producing organisms, showing high sensitivity and specificity for various carbapenemase genes including blaOXA-48, blaKPC, blaNDM, blaVIM, blaIMP, blaOXA-23, blaOXA-24/-40, and blaOXA-58.
Molecular and phenotypical characterization of two cases of antibiotic-driven ceftazidime-avibactam resistance in bla (KPC-3)-harboring Klebsiella pneumoniae.
The study identifies the D179Y mutation in the bla KPC-3 gene as a mechanism of ceftazidime-avibactam resistance in KPC-3-producing Klebsiella pneumoniae, leading to reduced avibactam binding and carbapenemase activity. Additionally, inactivation of the mgrB gene via IS Kpn26 insertion was found to confer colistin resistance.
Multidrug-Resistant Klebsiella pneumoniae ST307 in Traveler Returning from Puerto Rico to Dominican Republic
The study reports a multidrug-resistant Klebsiella pneumoniae ST307 isolate carrying blaKPC-2, CTX-M-15, SHV-100, OXA-1, and TEM-1D genes, highlighting the emergence of highly drug-resistant clones in the Americas.
Pseudomonas aeruginosa Coharboring Bla(KPC-2) and Bla(VIM-2) Carbapenemase Genes.
The study reports the first case series of Pseudomonas aeruginosa infections that concurrently harbor bla(VIM-2) and bla(KPC-2) carbapenemase genes, which confer resistance to carbapenems.
Patient-to-Patient Transmission of Klebsiella pneumoniae Carbapenemase Variants with Reduced Ceftazidime-Avibactam Susceptibility.
The study reports patient-to-patient transmission of Enterobacter hormaechei isolates with reduced susceptibility to ceftazidime-avibactam due to the production of KPC-40, a variant of KPC-3 with a two-amino-acid insertion in the Ω-loop region. Additionally, KPC-31, a variant of KPC-3 with a D179Y substitution, was also identified.
Patient-to-Patient Transmission of Klebsiella pneumoniae Carbapenemase Variants with Reduced Ceftazidime-Avibactam Susceptibility.
The study reports patient-to-patient transmission of Enterobacter hormaechei isolates with reduced susceptibility to ceftazidime-avibactam due to the production of KPC-40, a variant of KPC-3 with a two-amino-acid insertion in the Ω-loop region. Additionally, KPC-31, a variant of KPC-3 with a D179Y substitution, was also identified.
Patient-to-Patient Transmission of Klebsiella pneumoniae Carbapenemase Variants with Reduced Ceftazidime-Avibactam Susceptibility.
Patient-to-Patient Transmission of Klebsiella pneumoniae Carbapenemase Variants with Reduced Ceftazidime-Avibactam Susceptibility.
Patient-to-Patient Transmission of Klebsiella pneumoniae Carbapenemase Variants with Reduced Ceftazidime-Avibactam Susceptibility.
Patient-to-Patient Transmission of Klebsiella pneumoniae Carbapenemase Variants with Reduced Ceftazidime-Avibactam Susceptibility.
Patient-to-Patient Transmission of Klebsiella pneumoniae Carbapenemase Variants with Reduced Ceftazidime-Avibactam Susceptibility.
Managing All the Genotypic Knowledge: Approach to a Septic Patient Colonized by Different Enterobacteriales with Unique Carbapenemases.
The study characterizes the presence of bla OXA-48 and bla KPC genes in a patient infected with Raoultella planticola, highlighting the complexity of managing infections caused by multidrug-resistant organisms.
Diagnostic performance of the Xpert Carba-R assay for active surveillance of rectal carbapenemase-producing organisms in intensive care unit patients.
The study evaluated the diagnostic performance of the Xpert Carba-R assay for detecting rectal carbapenemase-producing organisms (CPOs) in ICU patients, identifying bla KPC, bla NDM, bla VIM, bla IMP-1, and bla OXA-48 as the main carbapenemase genes associated with resistance to carbapenems.
Molecular Epidemiology of Multidrug-Resistant Klebsiella pneumoniae Isolates in a Brazilian Tertiary Hospital.
The study identified various AMR genes and mutations in multidrug-resistant Klebsiella pneumoniae isolates, including bla KPC, bla CTX-M, bla TEM, and mutations in ompk35, ompk36, gyrA, and parC. These findings highlight the complex resistance mechanisms contributing to the persistence of MDR-Kp in the hospital setting.
Tracking Carbapenem-Producing Klebsiella pneumoniae Outbreak in an Intensive Care Unit by Whole Genome Sequencing.
The study identified multiple carbapenem-resistant Klebsiella pneumoniae (CP-Kp) strains carrying various resistance genes, including blaKPC-2, blaNDM-1, and others, contributing to multidrug resistance. Plasmid analysis revealed the presence of resistance genes on different plasmids, highlighting the complexity of resistance mechanisms.
High Prevalence of Metallo-β-Lactamase-Producing Enterobacter cloacae From Three Tertiary Hospitals in China.
The study identified multiple metallo-beta-lactamase genes, including blaNDM-1, blaIMP-26, blaIMP-4, blaIMP-1, blaVIM-4, and blaKPC-2, as major contributors to carbapenem resistance in Enterobacter cloacae isolates from three hospitals in China.
Whole-Genome Sequencing To Identify Drivers of Carbapenem-Resistant Klebsiella pneumoniae Transmission within and between Regional Long-Term Acute-Care Hospitals.
The study identified the blaKPC-1 gene as a key driver of carbapenem resistance in Klebsiella pneumoniae, contributing to the spread of resistant strains within and between healthcare facilities.
Evolution of Outbreak-Causing Carbapenem-Resistant Klebsiella pneumoniae ST258 at a Tertiary Care Hospital over 8 Years.
The study identifies various AMR genes in ST258 K. pneumoniae isolates, including blaKPC-2, blaKPC-3, aadA2, aadA1, blaTEM-1A, blaSHV-11, blaSHV-12, oqxA, oqxB, fosA, dfrA12, dfrA14, sul1, sul2, catA1, cml, and strAB, which confer resistance to multiple antibiotics.
Evolution of Outbreak-Causing Carbapenem-Resistant Klebsiella pneumoniae ST258 at a Tertiary Care Hospital over 8 Years.
The study identifies various AMR genes in ST258 K. pneumoniae isolates, including blaKPC-2, blaKPC-3, aadA2, aadA1, blaTEM-1A, blaSHV-11, blaSHV-12, oqxA, oqxB, fosA, dfrA12, dfrA14, sul1, sul2, catA1, cml, and strAB, which confer resistance to multiple antibiotics.
Characterization of a Multidrug-Resistant Porcine Klebsiella pneumoniae Sequence Type 11 Strain Coharboring bla(KPC-2) and fosA3 on Two Novel Hybrid Plasmids.
The study identifies the presence of blaKPC-2 and fosA3 on two novel hybrid plasmids in a multidrug-resistant porcine Klebsiella pneumoniae ST11 strain, highlighting the potential for transmission between animals and humans.
Phenotypic, biochemical and genetic analysis of KPC-41, a KPC-3 variant conferring resistance to ceftazidime-avibactam and exhibiting reduced carbapenemase activity.
The study identifies KPC-41, a novel KPC-3 variant that confers resistance to ceftazidime-avibactam and exhibits reduced carbapenemase activity.
Phenotypic, biochemical and genetic analysis of KPC-41, a KPC-3 variant conferring resistance to ceftazidime-avibactam and exhibiting reduced carbapenemase activity.
Phenotypic, biochemical and genetic analysis of KPC-41, a KPC-3 variant conferring resistance to ceftazidime-avibactam and exhibiting reduced carbapenemase activity.
Phenotypic, biochemical and genetic analysis of KPC-41, a KPC-3 variant conferring resistance to ceftazidime-avibactam and exhibiting reduced carbapenemase activity.
Phenotypic, biochemical and genetic analysis of KPC-41, a KPC-3 variant conferring resistance to ceftazidime-avibactam and exhibiting reduced carbapenemase activity.
Isolation and characterization of a sequence type 25 carbapenem-resistant hypervirulent Klebsiella pneumoniae from the mid-south region of China.
The study identified two carbapenemase genes, bla KPC-2 and bla NDM-1, in 20 carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) isolates. These genes were found to confer resistance to imipenem.
Antimicrobial Resistance Mechanisms in Antibiotic-Producing Bacteria and Pathogens
The paper discusses various antimicrobial resistance mechanisms in antibiotic-producing bacteria and pathogens, focusing on genes and mutations that confer resistance to different classes of antibiotics, including beta-lactams, aminoglycosides, tetracyclines, chloramphenicol, macrolides, and others.
An Outbreak of Carbapenem-Resistant and Hypervirulent Klebsiella pneumoniae in an Intensive Care Unit of a Major Teaching Hospital in Wenzhou, China.
The study identified carbapenem-resistant and hypervirulent Klebsiella pneumoniae isolates carrying bla KPC−2, aac(3')-Ia, aac(6')-Ib, ANT(3')-Ia, rmtB, rmtC, qnrB, qnrS, oqxA, bla SHV−11, bla CTX−M−2 group, bla CTX−M−9 group, bla TEM−1, bla CMY−2, and bla DHA genes, contributing to multidrug resistance.
High rate of multiresistant Klebsiella pneumoniae from human and animal origin.
The study identified multiple AMR genes, including blaKPC, blaNDM, blaTEM, blaSHV, qnrA, and tolC, in Klebsiella pneumoniae isolates from humans and animals in Henan, China. These genes were associated with resistance to various antibiotics, highlighting the prevalence of multidrug-resistant strains.
Emergence and Characterization of a Novel IncP-6 Plasmid Harboring bla (KPC-2) and qnrS2 Genes in Aeromonas taiwanensis Isolates.
The study identifies a novel IncP-6 plasmid, pKPC-1713, harboring bla(KPC-2) and qnrS2 genes in Aeromonas taiwanensis isolates, highlighting the co-occurrence of carbapenem and quinolone resistance genes on a non-conjugative plasmid.
Risk factors and survival of patients infected with carbapenem-resistant Klebsiella pneumoniae in a KPC endemic setting: a case-control and cohort study.
The study identified blaKPC-3 and blaKPC-2 as the primary carbapenemase genes in carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates, highlighting their role in carbapenem resistance.
Risk factors and survival of patients infected with carbapenem-resistant Klebsiella pneumoniae in a KPC endemic setting: a case-control and cohort study.
The study identified blaKPC-3 and blaKPC-2 as the primary carbapenemase genes in carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates, highlighting their role in carbapenem resistance.
Genomic characterization of a Klebsiella pneumoniae ST1519 resistant to ceftazidime/avibactam carrying a novel KPC variant (KPC-36).
Genomic characterization of a Klebsiella pneumoniae ST1519 resistant to ceftazidime/avibactam carrying a novel KPC variant (KPC-36).
Genomic characterization of a Klebsiella pneumoniae ST1519 resistant to ceftazidime/avibactam carrying a novel KPC variant (KPC-36).
Genomic characterization of a Klebsiella pneumoniae ST1519 resistant to ceftazidime/avibactam carrying a novel KPC variant (KPC-36).
Outbreak Of Klebsiella pneumoniae Carbapenemase-Producing Klebsiella aerogenes Strains In A Tertiary Hospital In China.
The study identified blaKPC-2 as the primary carbapenem resistance mechanism in carbapenem-resistant Klebsiella aerogenes (CRKA) isolates, with the gene located on plasmids belonging to various incompatibility groups.
Molecular characterization of carbapenem-resistant Klebsiella pneumoniae isolates with focus on antimicrobial resistance.
The study identifies blaKPC-2 as the dominant carbapenemase gene in clinical CRKP isolates, along with various ESBLs and other resistance genes such as blaCTX-M, blaTEM, blaSHV, aac(3)-IId, rmtB, QnrS1, oqxA, oqxB, fosA, catA1, catA2, dfrA1, and dfrA17.
Environmental Presence and Genetic Characteristics of Carbapenemase-Producing Enterobacteriaceae from Hospital Sewage and River Water in the Philippines.
The study identified NDM, KPC, OXA-48-like, GES, and IMI carbapenemase genes in carbapenemase-producing Enterobacteriaceae (CPE) isolated from hospital sewage and river water in the Philippines. NDM was the most prevalent gene type, followed by KPC, OXA-48-like, GES, and IMI. Additionally, CTX-M-type ESBL and 16S rRNA methylase genes were also detected.
The role of the bla (KPC) gene in antimicrobial resistance of Klebsiella pneumoniae.
The study identified the blaKPC gene as a significant contributor to carbapenem resistance in Klebsiella pneumoniae isolates, with all five blaKPC-positive isolates showing resistance to meropenem and colistin.
Treatment Options for Carbapenem-resistant Gram-negative Bacterial Infections.
The paper discusses various carbapenem-resistant gram-negative pathogens and highlights the importance of new antimicrobial agents like ceftazidime-avibactam, meropenem-vaborbactam, and others in treating infections caused by these resistant organisms.
Evaluation of eazyplex(®) SuperBug CRE Test for Beta-Lactamase Genes Detection in Klebsiella spp. and P. aeruginosa Strains.
The study evaluated the eazyplex® SuperBug CRE test for detecting beta-lactamase genes in Klebsiella spp. and P. aeruginosa, identifying several resistance genes including blaCTX-M1, blaCTX-M9, blaVIM, blaNDM, blaKPC, and blaOXA-48.
Use of carbapenems in the combined treatment of emerging ceftazidime/avibactam-resistant and carbapenem-susceptible KPC-producing Klebsiella pneumoniae infections: Report of a case and review of the literature.
The study reports a case of a patient infected with a KPC-producing Klebsiella pneumoniae isolate that developed resistance to ceftazidime/avibactam while remaining susceptible to carbapenems. The isolate carried various KPC variants including KPC-3, KPC-39, and KPC-48.
Use of carbapenems in the combined treatment of emerging ceftazidime/avibactam-resistant and carbapenem-susceptible KPC-producing Klebsiella pneumoniae infections: Report of a case and review of the literature.
The study reports a case of a patient infected with a KPC-producing Klebsiella pneumoniae isolate that developed resistance to ceftazidime/avibactam while remaining susceptible to carbapenems. The isolate carried various KPC variants including KPC-3, KPC-39, and KPC-48.
Use of carbapenems in the combined treatment of emerging ceftazidime/avibactam-resistant and carbapenem-susceptible KPC-producing Klebsiella pneumoniae infections: Report of a case and review of the literature.
The study reports a case of a patient infected with a KPC-producing Klebsiella pneumoniae isolate that developed resistance to ceftazidime/avibactam while remaining susceptible to carbapenems. The isolate carried various KPC variants including KPC-3, KPC-39, and KPC-48.
Use of carbapenems in the combined treatment of emerging ceftazidime/avibactam-resistant and carbapenem-susceptible KPC-producing Klebsiella pneumoniae infections: Report of a case and review of the literature.
The study reports a case of a patient infected with a KPC-producing Klebsiella pneumoniae isolate that developed resistance to ceftazidime/avibactam while remaining susceptible to carbapenems. The isolate carried various KPC variants including KPC-3, KPC-39, and KPC-48.
Use of carbapenems in the combined treatment of emerging ceftazidime/avibactam-resistant and carbapenem-susceptible KPC-producing Klebsiella pneumoniae infections: Report of a case and review of the literature.
Use of carbapenems in the combined treatment of emerging ceftazidime/avibactam-resistant and carbapenem-susceptible KPC-producing Klebsiella pneumoniae infections: Report of a case and review of the literature.
CRISPR-Cas influences the acquisition of antibiotic resistance in Klebsiella pneumoniae.
The study shows that CRISPR-Cas systems in Klebsiella pneumoniae inhibit the acquisition of blaKPC-2 and blaKPC-3 plasmids, thereby reducing carbapenem resistance. CRISPR-Cas positive strains are more susceptible to carbapenems and other antibiotics compared to CRISPR-Cas negative strains.
CRISPR-Cas influences the acquisition of antibiotic resistance in Klebsiella pneumoniae.
The study shows that CRISPR-Cas systems in Klebsiella pneumoniae inhibit the acquisition of blaKPC-2 and blaKPC-3 plasmids, thereby reducing carbapenem resistance. CRISPR-Cas positive strains are more susceptible to carbapenems and other antibiotics compared to CRISPR-Cas negative strains.
Characterization of Klebsiella pneumoniae ST11 Isolates and Their Interactions with Lytic Phages.
The study characterizes Klebsiella pneumoniae ST11 isolates carrying the blaKPC-2 gene, which confers resistance to carbapenems. The research also explores the effectiveness of phage therapy against these isolates.
Characterization of Klebsiella pneumoniae ST11 Isolates and Their Interactions with Lytic Phages.
The study characterizes Klebsiella pneumoniae ST11 isolates carrying the blaKPC-2 gene, which confers resistance to carbapenems. The research also explores the effectiveness of phage therapy against these isolates.
The Rapid Carbapenemase Detection Method (rCDM) for Rapid and Accurate Detection of Carbapenemase-Producing Enterobacteriaceae and Pseudomonas aeruginosa.
The study developed the rapid carbapenemase detection method (rCDM) for detecting carbapenemase-producing Enterobacteriaceae and Pseudomonas aeruginosa. The method showed high sensitivity and specificity for detecting various carbapenemase genes, including blaKPC-2, blaIMP-4, blaVIM-1, blaNDM-1, blaOXA-48, and blaVIM-4.
Development of a Multiplex PCR Platform for the Rapid Detection of Bacteria, Antibiotic Resistance, and Candida in Human Blood Samples.
The study describes a multiplex PCR platform called MicrobScan for the rapid detection of bacteria, Candida, and antibiotic resistance genes in human blood samples. It successfully detected 20 bacterial and four Candida species, as well as three antibiotic-resistance determinants: blaKPC, mecA, and vanA/vanB.
Genomic characterization of an emerging bla(KPC-2) carrying Enterobacteriaceae clinical isolates in Thailand.
The study describes the first characterization of blaKPC-2 carrying Klebsiella pneumoniae, Escherichia coli, and Enterobacter asburiae isolates in Thailand, highlighting their resistance to carbapenems and other antibiotics.
Post-neurosurgical meningitis caused by KPC-producing Klebsiella pneumoniae: report of two cases.
Two cases of post-neurosurgical meningitis caused by KPC-producing Klebsiella pneumoniae were reported. The isolates were found to produce bla-KPC, bla-SHV, and bla-TEM genes, leading to resistance against beta-lactam antibiotics.
VNRX-5133 (Taniborbactam), a Broad-Spectrum Inhibitor of Serine- and Metallo-beta-lactamases, Restores Activity of Cefepime in Enterobacterales and Pseudomonas aeruginosa.
Taniborbactam is a broad-spectrum beta-lactamase inhibitor that restores the activity of cefepime against Enterobacterales and Pseudomonas aeruginosa producing various beta-lactamases, including serine and metallo-beta-lactamases.
First report of Klebsiella quasipneumoniae harboring bla(KPC-2) in Saudi Arabia.
The study reports the first identification of a KPC-2-producing Klebsiella quasipneumoniae subsp. similipneumoniae strain in the Gulf region, highlighting the presence of the blaKPC-2 gene on an IncX6 plasmid.
Fecal Carriage and Epidemiology of Carbapenem-Resistant Enterobacteriaceae Among Hospitalized Patients in a University Hospital.
The study identified KPC, NDM, and IMP carbapenemases as the primary resistance mechanisms in CRE isolates, with KPC being the most prevalent. No mcr-1 gene was detected in the CRE isolates.
The transferability and evolution of NDM-1 and KPC-2 co-producing Klebsiella pneumoniae from clinical settings.
The study identified KPC-2-NDM-1-CRKP isolates with high stability and transferability, revealing their evolutionary pathway and highlighting the need for surveillance and infection control measures.
Development and Evaluation of a Novel Protein-Based Assay for Specific Detection of KPC beta-lactamases from Klebsiella pneumoniae Clinical Isolates.
The study developed a protein-based assay using BLIP K74T/W112D to specifically detect KPC-2 beta-lactamases in clinical isolates of Klebsiella pneumoniae, demonstrating 100% concordance with genome sequencing results.
IS26-Mediated Transfer of bla (NDM-1) as the Main Route of Resistance Transmission During a Polyclonal, Multispecies Outbreak in a German Hospital.
The study identifies blaNDM-1 and blaKPC-2 as the main resistance genes responsible for carbapenem resistance in a polyclonal, multispecies outbreak in a German hospital. The genes were found to be located on plasmids and associated with IS26 elements facilitating horizontal gene transfer.
VAMPr: VAriant Mapping and Prediction of antibiotic resistance via explainable features and machine learning.
VAMPr identifies AMR genes and variants through association and prediction models, confirming known resistance mechanisms like blaKPC and oprD, and detecting novel variants.
Impact of anticancer chemotherapy on the extension of beta-lactamase spectrum: an example with KPC-type carbapenemase activity towards ceftazidime-avibactam.
Anticancer chemotherapy increases the mutation rate of KPC-type carbapenemase-producing Enterobacteriaceae, leading to resistance against ceftazidime-avibactam. Several mutations in the omega-loop of the blaKPC gene were identified as contributing to this resistance.
Ceftazidime-Avibactam Resistance Associated with Increased blaKPC-3 Gene Copy Number Mediated by pKpQIL Plasmid Derivatives in Sequence Type 258 Klebsiella pneumoniae.
The study identified that increased blaKPC-3 gene copy number mediated by pKpQIL plasmid derivatives contributes to ceftazidime-avibactam resistance in sequence type 258 Klebsiella pneumoniae.
High-performance method to detection of Klebsiella pneumoniae Carbapenemase in Enterobacterales by LC-MS/MS.
The study validated a high-performance LC-MS/MS method for detecting Klebsiella pneumoniae carbapenemase (KPC) in Enterobacterales, demonstrating high sensitivity and specificity.
Outbreak of KPC-2-producing Klebsiella pneumoniae endowed with ceftazidime-avibactam resistance mediated through a VEB-1-mutant (VEB-25), Greece, September to October 2019.
Evaluation of the Revogene Carba C Assay for Detection and Differentiation of Carbapenemase-Producing Gram-Negative Bacteria.
The Revogene Carba C assay effectively detects the five major carbapenemases (NDM, VIM, IMP, KPC, and OXA-48) in Enterobacterales, Pseudomonas aeruginosa, and Acinetobacter baumannii, demonstrating high sensitivity and specificity.
Population structure of KPC carbapenemase-producing Klebsiella pneumoniae in a long-term acute-care rehabilitation facility: identification of a new lineage of clonal group 101, associated with local hyperendemicity.
The study identifies a new lineage of KPC-producing Klebsiella pneumoniae (ST2502) associated with hyperendemicity in a long-term acute-care rehabilitation facility. Key AMR genes include armA, blaKPC-3, blaKPC-2, and aac6-Ib, with pmrA mutations contributing to colistin resistance.
Population structure of KPC carbapenemase-producing Klebsiella pneumoniae in a long-term acute-care rehabilitation facility: identification of a new lineage of clonal group 101, associated with local hyperendemicity.
The study identifies a new lineage of KPC-producing Klebsiella pneumoniae (ST2502) associated with hyperendemicity in a long-term acute-care rehabilitation facility. Key AMR genes include armA, blaKPC-3, blaKPC-2, and aac6-Ib, with pmrA mutations contributing to colistin resistance.
Potency of Vaborbactam Is Less Affected than That of Avibactam in Strains Producing KPC-2 Mutations That Confer Resistance to Ceftazidime-Avibactam.
The study identifies D179Y and L169P mutations in KPC-2 that reduce avibactam efficacy and increase resistance to ceftazidime, highlighting differences in the effectiveness of vaborbactam against these variants.
Emergence of ST11-K47 and ST11-K64 hypervirulent carbapenem-resistant Klebsiella pneumoniae in bacterial liver abscesses from China: a molecular, biological, and epidemiological study.
The study identifies the emergence of ST11-K47 and ST11-K64 hypervirulent carbapenem-resistant Klebsiella pneumoniae strains in China, highlighting their multidrug resistance and high virulence.
Novel Cassette Assay To Quantify the Outer Membrane Permeability of Five β-Lactams Simultaneously in Carbapenem-Resistant Klebsiella pneumoniae and Enterobacter cloacae.
The study identifies various β-lactamase genes, including bla KPC-2, bla TEM-1B, bla SHV-182, bla OXA-1, bla CTX-M-15, bla ACT-7, bla CTX-M-8, and bla OXA-9, which contribute to β-lactam resistance in carbapenem-resistant Klebsiella pneumoniae and Enterobacter cloacae isolates.
Coexistence of the bla(NDM-1)-carrying plasmid pWLK-NDM and the bla(KPC-2)-carrying plasmid pWLK-KPC in a Raoultella ornithinolytica isolate.
The study reports the first environmental isolate of Raoultella ornithinolytica co-harboring the bla(NDM-1) and bla(KPC-2) genes, highlighting the potential for environmental dissemination of these carbapenem resistance genes.
Should we leave the paper currency? A microbiological examination.
The study identified various antimicrobial resistance genes including mecA, vanA, blaTEM, blaSHV, blaCTX-M-1, blaCTX-M-2, blaKPC, and blaOXA-48 in bacteria isolated from Turkish banknotes, highlighting the potential role of paper currency in spreading pathogens and antimicrobial resistance.
Acquisition of Plasmid with Carbapenem-Resistance Gene bla(KPC2) in Hypervirulent Klebsiella pneumoniae, Singapore.
The study identifies the plasmid pKPC2 carrying the carbapenem-resistance gene blaKPC-2 in hypervirulent Klebsiella pneumoniae isolates in Singapore, highlighting its stability and transmissibility.
Meropenem-Vaborbactam versus Ceftazidime-Avibactam for Treatment of Carbapenem-Resistant Enterobacteriaceae Infections.
The study compared the effectiveness of meropenem-vaborbactam (MVB) and ceftazidime-avibactam (CZA) in treating carbapenem-resistant Enterobacteriaceae (CRE) infections. It found that CZA resistance developed in some patients, while MVB resistance was not observed during the study period.
Resistome in Lake Bolonha, Brazilian Amazon: Identification of Genes Related to Resistance to Broad-Spectrum Antibiotics.
The study identified several AMR genes in Lake Bolonha, including bla CTX-M, bla SHV, bla TEM, bla VIM, bla IMP, intl1, and intl2, which confer resistance to beta-lactams and other antibiotics. These genes were found in various bacterial isolates, highlighting the presence of multidrug-resistant bacteria in the lake.
Development of a Two Triplex Real-Time Polymerase Chain Reaction for Rapid Detection of Six Carbapenemase Genes in Enterobacteriaceae.
The study developed a two triplex real-time PCR assay for the rapid detection of six carbapenemase genes (blaKPC, blaNDM, blaOXA-48_like, blaIMP, blaVIM, and blaGES) in Enterobacteriaceae, demonstrating 100% concordance with previously identified genotypes.
Rapid Detection of KPC-Producing Enterobacterales Susceptible to Imipenem/Relebactam by Using the MALDI-TOF MS MBT STAR-Carba IVD Assay.
The study presents a MALDI-TOF MS-based method for rapidly detecting KPC-producing Enterobacterales susceptible to imipenem/relebactam, showing high sensitivity and specificity.
Evaluation of Phenotypic and Genotypic Characteristics of Carbapnemases-producing Enterobacteriaceae and Its Prevalence in a Referral Hospital in Tehran City.
The study identified the prevalence of carbapenem-resistant Enterobacteriaceae in a referral hospital in Tehran, Iran, highlighting the significance of OXA-48, NDM, and KPC genes as major contributors to carbapenem resistance.
Value of multiplex PCR for detection of antimicrobial resistance in samples retrieved from patients with orthopaedic infections.
The study evaluated the performance of multiplex PCR (mPCR) for detecting antimicrobial resistance genes in clinical samples from patients with orthopaedic infections. It identified several resistance genes, including mecA, mecC, aac(6')/aph(2''), ermA, ermC, rpoB, vanA, vanB, ctx-M, blaNDM, blaOXA-23, blaOXA-24, blaOXA-48, blaOXA-58, blaIMP, blaKPC, gyrA83, gyrA87, and aacA4, which confer resistance to various antibiotics such as oxacillin, aminoglycosides, macrolides/lincosamides, rifampin, vancomycin, third-generation cephalosporins, carbapenems, and fluoroquinolones.
Genomic analysis of 40 prophages located in the genomes of 16 carbapenemase-producing clinical strains of Klebsiella pneumoniae.
The study identified 40 prophages in 16 carbapenemase-producing clinical strains of Klebsiella pneumoniae, highlighting the presence of beta-lactamase genes (blaOXA-48, blaVIM-1, blaKPC) and mcr genes associated with carbapenem and colistin resistance.
The type I-E CRISPR-Cas system influences the acquisition of bla(KPC)-IncF plasmid in Klebsiella pneumonia.
The study shows that the type I-E CRISPR-Cas system in Klebsiella pneumoniae can hinder the acquisition and stability of bla(KPC)-IncF plasmids, indicating that the scarcity of CRISPR-Cas systems in CG258 lineage may facilitate the spread of these plasmids.
Gram-negative bacteria carrying β-lactamase encoding genes in hospital and urban wastewater in Brazil.
The study identified β-lactamase encoding genes, including bla KPC, bla TEM, bla SHV, and bla CTX-M, in Gram-negative bacteria from hospital and urban wastewater in Brazil, highlighting the presence of multidrug-resistant strains.
Emergence of β-lactamase- and carbapenemase- producing Enterobacteriaceae at integrated fish farms.
The study identified several β-lactamase and carbapenemase genes, including bla KPC, bla OXA-48, bla NDM, bla CTX-M-15, bla SHV, bla OXA-1, bla TEM, and bla PER-1, which confer resistance to cephalosporins and carbapenems in Enterobacteriaceae isolated from fish, water, and workers at integrated agriculture-aquaculture systems in Egypt.
Metagenomic Diagnosis for a Culture-Negative Sample From a Patient With Severe Pneumonia by Nanopore and Next-Generation Sequencing.
The study identified several AMR genes in a culture-negative sample from a patient with severe pneumonia, including bla SHV-12, bla KPC-2, bla TEM-1, bla CTX-M-65, aac(3)-IIa, aadA1, dfrA1, sul1, aph(3')-Ia, catA1, fosA, acrA, and rmtB, which confer resistance to various antibiotics.
Cointegration as a mechanism for the evolution of a KPC-producing multidrug resistance plasmid in Proteus mirabilis.
The study characterizes two carbapenemase-producing plasmids, pT18 and pT211, from Proteus mirabilis, highlighting the role of cointegrate plasmids in the dissemination of antibiotic resistance genes. The plasmids carry various resistance genes, including blaKPC-2, blaCTX-M-65, blaTEM-1B, rmtB, and fosA3, contributing to multidrug resistance.
KPC-50 Confers Resistance to Ceftazidime-Avibactam Associated with Reduced Carbapenemase Activity.
The study identifies KPC-50, a variant of KPC-3, which confers resistance to ceftazidime-avibactam and exhibits reduced carbapenemase activity.
KPC-50 Confers Resistance to Ceftazidime-Avibactam Associated with Reduced Carbapenemase Activity.
KPC-50 Confers Resistance to Ceftazidime-Avibactam Associated with Reduced Carbapenemase Activity.
In Vitro Activity of Essential Oils Against Planktonic and Biofilm Cells of Extended-Spectrum β-Lactamase (ESBL)/Carbapenamase-Producing Gram-Negative Bacteria Involved in Human Nosocomial Infections.
The study identified several beta-lactamase genes, including blaCTX-M-15, blaTEM-52, blaCTX-M-1, blaKPC-2, blaVIM-1, and blaVIM-2, which confer resistance to various beta-lactam antibiotics in ESBL and carbapenemase-producing Gram-negative bacteria.
CRISPR-based platform for carbapenemases and emerging viruses detection using Cas12a (Cpf1) effector nuclease.
The study demonstrates the use of CRISPR-Cas12a for detecting DNA and RNA targets, including carbapenemase resistance genes such as KPC, NDM, and OXA-48, as well as viral RNA sequences from DENV, ZIKV, and HANTV.
One year cross-sectional study in adult and neonatal intensive care units reveals the bacterial and antimicrobial resistance genes profiles in patients and hospital surfaces.
The study identified several antimicrobial resistance (AMR) genes, including mecA, bla CTX-M-1 group, bla SHV-like, bla KPC-like, and vanA, in both patient and hospital surface samples from adult and neonatal intensive care units (ICU and NICU). These genes were found to be prevalent in various bacterial species, highlighting the widespread nature of AMR in hospital environments.
Multicenter Evaluation of the Xpert Carba-R Assay for Detection and Identification of Carbapenemase Genes in Sputum Specimens.
The Xpert Carba-R assay effectively detects carbapenemase genes (bla KPC, bla NDM, bla IMP, and bla VIM) in sputum specimens, providing rapid and accurate identification of carbapenem-resistant Enterobacteriaceae.
Species Transferability of Klebsiella pneumoniae Carbapenemase-2 Isolated from a High-Risk Clone of Escherichia coli ST410.
The study identifies blaKPC-2 as a carbapenemase gene in E. coli ST410 and K. pneumoniae ST307, highlighting the horizontal transfer of resistance genes between species.
Development of CRISPR-Cas13a-based antimicrobials capable of sequence-specific killing of target bacteria.
The study demonstrates the development of CRISPR-Cas13a-based antimicrobials that can specifically kill bacteria carrying AMR genes such as blaIMP-1, mcr-1, mcr-2, blaOXA-48, blaVIM-2, blaNDM-1, blaKPC-2, and mecA.
Prediction of Antimicrobial Resistance in Gram-Negative Bacteria From Whole-Genome Sequencing Data.
The study presents machine learning models for predicting antimicrobial resistance in Gram-negative bacteria using whole-genome sequencing data. Key resistance genes identified include AAC(6')-Ib, APH(3")-Ib, OqxB21, FosA2, SHV-200, EC-18, CTX-M-222, KPC-33, OXA-51, and OXA-561.
Co-Occurrence of the bla (KPC-2) and Mcr-3.3 Gene in Aeromonas caviae SCAc2001 Isolated from Patients with Diarrheal Disease.
The study identifies multiple antimicrobial resistance genes in Aeromonas caviae SCAc2001, including blaKPC, mcr-3.3, and others, highlighting the potential public health risks posed by this strain.
Molecular Characteristics of Carbapenem-Resistant Enterobacter cloacae in a Tertiary Hospital in China.
The study identified four types of carbapenemases (IMP-26, NDM-5, NDM-1, and KPC-2) in 12 carbapenem-resistant Enterobacter cloacae strains, along with CTX-M-3 and DHA1 beta-lactamases.
Clinical and Genomic Epidemiology of Carbapenem-Nonsusceptible Citrobacter spp. at a Tertiary Health Care Center over 2 Decades.
The study identified blaKPC-3, blaNDM-1, and blaKPC-2 as the primary carbapenemase genes responsible for carbapenem resistance in Citrobacter species, highlighting the diversity and mobility of these resistance determinants.
Clinical and Genomic Epidemiology of Carbapenem-Nonsusceptible Citrobacter spp. at a Tertiary Health Care Center over 2 Decades.
The study identified blaKPC-3, blaNDM-1, and blaKPC-2 as the primary carbapenemase genes responsible for carbapenem resistance in Citrobacter species, highlighting the diversity and mobility of these resistance determinants.
Successful control of the first carbapenem-resistant Klebsiella pneumoniae outbreak in a Chinese hospital 2017-2019.
The study identified blaKPC-2 and blaNDM-1 as the primary carbapenem resistance genes in the CRKP outbreak, highlighting the need for targeted infection control measures.
Identification of plasmid IncQ1 and NTEKPC-IId harboring bla KPC-2 in isolates from Klebsiella pneumoniae infections in patients from Recife-PE, Brazil.
The study identifies the bla KPC-2 gene in non-Tn4401 (NTEKPC-IId) element carried by plasmid IncQ1 in four multidrug-resistant Klebsiella pneumoniae isolates from Brazil.
Association of CRISPR/Cas System with the Drug Resistance in Klebsiella pneumoniae.
The study identifies blaKPC, blaCTX-M, blaTEM, blaSHV, blaFOX, and blaDHA as key drug resistance genes in Klebsiella pneumoniae, with blaKPC being the main carbapenem resistance gene. The presence of the CRISPR/Cas system correlates with reduced drug resistance.
Detection of Carbapenem-Resistant Genes in Escherichia coli Isolated from Drinking Water in Khartoum, Sudan.
The study detected carbapenem-resistant genes bla OXA-48, bla SPM, and bla KPC in Escherichia coli isolates from drinking water in Khartoum, Sudan. bla OXA-48 was the most prevalent gene, followed by bla SPM and bla KPC. The isolates showed high resistance to tetracycline, gentamicin, ciprofloxacin, ceftriaxone, and chloramphenicol.
A coup d'état by NDM-producing Klebsiella pneumoniae overthrows the major bacterial population during KPC-directed therapy.
The study characterizes the emergence and dominance of a minor NDM-producing Klebsiella pneumoniae population during KPC-directed therapy, highlighting challenges in detecting and managing drug-resistant subpopulations.
Whole Genome Sequencing and Characterization of Multidrug-Resistant (MDR) Bacterial Strains Isolated From a Norwegian University Campus Pond.
The study identifies multiple AMR genes and mutations in ESBL-producing bacteria from Norwegian freshwater environments, highlighting the presence of multidrug-resistant strains with resistance to various antibiotics including beta-lactams, fluoroquinolones, and sulfonamides.
Distribution of Class B and Class A β-Lactamases in Clinical Strains of Pseudomonas aeruginosa: Comparison of Phenotypic Methods and High-Resolution Melting Analysis (HRMA) Assay.
The study identified various β-lactamase genes, including bla SHV, bla TEM, bla KPC, bla IMP, bla VIM, and bla GES, in Pseudomonas aeruginosa isolates using HRMA assay. These genes were associated with resistance to multiple β-lactam antibiotics.
Reduced Ceftazidime-Avibactam Susceptibility in KPC-Producing Klebsiella pneumoniae From Patients Without Ceftazidime-Avibactam Use History - A Multicenter Study in China.
The study identified bla KPC–2 as a gene responsible for reduced susceptibility to ceftazidime-avibactam in KPC-producing Klebsiella pneumoniae. Mutations in ompK35 and ompK36 were also associated with reduced susceptibility.
Resistance to Ceftazidime/Avibactam plus Meropenem/Vaborbactam When Both Are Used Together Is Achieved in Four Steps in Metallo-β-Lactamase-Negative Klebsiella pneumoniae.
Four genetic steps, including mutations in ramR and ompK36, and the acquisition of OXA-232, KPC-3, and KPC-3-D178Y, confer resistance to ceftazidime/avibactam and meropenem/vaborbactam in metallo-β-lactamase-negative Klebsiella pneumoniae.
Characterization of KPC-Producing Serratia marcescens in an Intensive Care Unit of a Brazilian Tertiary Hospital.
The study identified KPC-producing Serratia marcescens isolates with resistance to beta-lactams, including carbapenems, and the presence of aac(6')-Ib-cr, SdeB, SdeY, and HasF genes associated with resistance to aminoglycosides, fluoroquinolones, and tigecycline.
Synergistic effects of Cinnamomum cassia L. essential oil in combination with polymyxin B against carbapenemase-producing Klebsiella pneumoniae and Serratia marcescens.
The study identifies bla KPC-2 and arnB as resistance genes in carbapenemase-producing K. pneumoniae and S. marcescens, demonstrating their roles in carbapenem and polymyxin resistance, respectively.
Structure-based functional fitness analyses of carbapenemase variants identified among pathogenic carbapenem-resistant Gram-negative bacteria.
The study identified several carbapenemase variants, including blaNDM-1, blaVIM-2, blaKPC-2, blaOXA-181, blaOXA-23, blaOXA-66, blaOXA-69, and blaOXA-104, as critical determinants of carbapenem resistance in pathogenic Gram-negative bacteria.
Therapeutic Effect and Mechanisms of the Novel Monosulfactam 0073.
The novel monosulfactam 0073 showed potent activity against multidrug-resistant Gram-negative bacteria, including those producing various beta-lactamases such as KPC-2, NDM-1, TEM-1, OXA-48, SHV-11, CTX-M-1, and CIT. It exhibited lower MICs compared to aztreonam and had reduced resistance development potential.
Emergence of ceftazidime-avibactam resistance through distinct genomic adaptations in KPC-2-producing Klebsiella pneumoniae of sequence type 39 during treatment.
Emergence of ceftazidime-avibactam resistance through distinct genomic adaptations in KPC-2-producing Klebsiella pneumoniae of sequence type 39 during treatment.
Emerging Antimicrobial-Resistant High-Risk Klebsiella pneumoniae Clones ST307 and ST147.
The study identifies and characterizes the antimicrobial resistance genes and mutations associated with the high-risk Klebsiella pneumoniae clones ST307 and ST147, highlighting their global spread and the diversity of resistance mechanisms they employ.
Emerging Antimicrobial-Resistant High-Risk Klebsiella pneumoniae Clones ST307 and ST147.
The study identifies and characterizes the antimicrobial resistance genes and mutations associated with the high-risk Klebsiella pneumoniae clones ST307 and ST147, highlighting their global spread and the diversity of resistance mechanisms they employ.
Clinical and Molecular Epidemiologic Characteristics of Ceftazidime/Avibactam-Resistant Carbapenem-Resistant Klebsiella pneumoniae in a Neonatal Intensive Care Unit in China.
The study identified bla_kpc-2, bla_ndm-1, and bla_ndm-5 as the primary carbapenemase genes responsible for ceftazidime/avibactam resistance in carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates from a neonatal intensive care unit in China. Additionally, shv-11 and ctx-m-14 were found to contribute to extended-spectrum beta-lactamase activity.
Emergence of Hypervirulent Ceftazidime/Avibactam-Resistant Klebsiella pneumoniae Isolates in a Chinese Tertiary Hospital.
The study identifies the emergence of ceftazidime/avibactam-resistant hypervirulent Klebsiella pneumoniae isolates, primarily associated with the presence of metallo-beta-lactamases (NDM-1, NDM-5), KPC-2, and DHA-1 beta-lactamases, as well as the loss of porin genes ompK35 and ompK36.
Antimicrobial Resistance Caused by KPC-2 Encoded by Promiscuous Plasmids of the Klebsiella pneumoniae ST307 Strain.
The study identifies the blaKPC-2 gene as a key contributor to carbapenem resistance in Klebsiella pneumoniae ST307, highlighting its horizontal transfer capability among Enterobacteriaceae species.
A Ceftazidime-Avibactam-Resistant and Carbapenem-Susceptible Klebsiella pneumoniae Strain Harboring bla(KPC-14) Isolated in New York City.
The study reports a novel bla KPC variant, bla KPC-14, which confers resistance to ceftazidime-avibactam but not to carbapenems. The gene was identified in a K. pneumoniae strain isolated in 2003, predating the introduction of avibactam.
ChromID(®) CARBA Agar Fails to Detect Carbapenem-Resistant Enterobacteriaceae With Slightly Reduced Susceptibility to Carbapenems.
The study identifies that ChromID(®) CARBA agar fails to detect carbapenem-resistant Enterobacteriaceae with slightly reduced susceptibility to carbapenems, highlighting the need for alternative selective media such as MacConkey agar supplemented with cefotaxime and meropenem for reliable detection.
Antimicrobial Stewardship Program, COVID-19, and Infection Control: Spread of Carbapenem-Resistant Klebsiella Pneumoniae Colonization in ICU COVID-19 Patients. What Did Not Work?
The study highlights the spread of carbapenem-resistant Klebsiella pneumoniae (CR-Kp) in an ICU during the COVID-19 pandemic, identifying various carbapenemase genes including KPC, OXA-48-like, OXA-163, NDM, and VIM as contributors to resistance.
Predicting β-lactam resistance using whole genome sequencing in Klebsiella pneumoniae: the challenge of beta-lactamase inhibitors.
The study identifies several beta-lactamase genes, including bla NDM-1, bla OXA-48, bla KPC-8, and bla KPC-14, which confer resistance to various β-lactam antibiotics. It also highlights the importance of considering complex beta-lactamase backgrounds when predicting antimicrobial resistance using whole-genome sequencing.
Predicting β-lactam resistance using whole genome sequencing in Klebsiella pneumoniae: the challenge of beta-lactamase inhibitors.
The study identifies several beta-lactamase genes, including bla NDM-1, bla OXA-48, bla KPC-8, and bla KPC-14, which confer resistance to various β-lactam antibiotics. It also highlights the importance of considering complex beta-lactamase backgrounds when predicting antimicrobial resistance using whole-genome sequencing.
Rapid Detection and Characterization of Carbapenemases in Enterobacterales with a New Modified Carbapenem Inactivation Method, mCIMplus.
The study evaluates the mCIMplus test for rapid detection and characterization of carbapenemases in Enterobacterales, demonstrating high sensitivity and specificity for various carbapenemase types including OXA-48-like, NDM, KPC, VIM, GES-5, and IMP-1.
Application and clinical impact of the RESIST-4 O.K.N.V. rapid diagnostic test for carbapenemase detection in blood cultures and clinical samples.
The RESIST-4 O.K.N.V. RDT effectively detects KPC-3 and OXA-48 carbapenemases in clinical samples, enabling rapid treatment modifications to ceftazidime/avibactam-containing regimens.
Prevalence and Distribution Characteristics of bla(KPC-2) and bla(NDM-1) Genes in Klebsiella pneumoniae.
The study identified bla(KPC-2) and bla(NDM-1) genes as the primary contributors to carbapenem resistance in Klebsiella pneumoniae, highlighting their prevalence and distribution in clinical isolates from a Chinese hospital and global genomes.
Risk Factors Associated with Carbapenemase-Producing Enterobacterales (CPE) Positivity in the Hospital Wastewater Environment.
The study identifies blaKPC as a gene responsible for carbapenem resistance in KPCO-positive patients and environmental isolates, highlighting its role in hospital wastewater environments.
Dynamics of blaKPC-2 Dissemination from Non-CG258 Klebsiella pneumoniae to Other Enterobacterales via IncN Plasmids in an Area of High Endemicity.
The study characterizes the dissemination of blaKPC-2 among various Enterobacterales species via IncN plasmids, highlighting the role of horizontal gene transfer in the spread of carbapenem resistance in a high-endemicity area in Colombia.
In Vitro Activity of Cefiderocol, a Siderophore Cephalosporin, against Multidrug-Resistant Gram-Negative Bacteria.
Cefiderocol shows good in vitro activity against multidrug-resistant Gram-negative bacteria, including those with NDM, VIM, IMP, KPC, OXA-48-like, GES, IMI, SME, VEB, PER, OXA-23, OXA-24/40, OXA-51, and OXA-58 carbapenemases.
Using Therapeutic Drug Monitoring to Treat KPC-Producing Klebsiella pneumoniae Central Nervous System Infection With Ceftazidime/Avibactam.
The study identifies multiple AMR genes in a KPC-3-producing K. pneumoniae isolate, including blaKPC-3, aac(3)-Ib, aac(6')-Ib-cr, aph(3')-Ia, blaOXA-1, blaSHV-214, mphA, qacH, catB3, arr-3, sul1, and dfrA14, which confer resistance to various antibiotics.
Epidemiology and risk factors of rectal colonization of carbapenemase-producing Enterobacteriaceae among high-risk patients from ICU and HSCT wards in a university hospital.
The study identified bla KPC and bla NDM as the primary carbapenemase genes responsible for carbapenem resistance in Enterobacteriaceae isolates from high-risk patients in ICU and HSCT wards. bla KPC was predominantly found in Klebsiella pneumoniae, while bla NDM was prevalent in Escherichia coli and other species.
Genetic factors related to the widespread dissemination of ST11 extensively drug-resistant carbapenemase-producing Klebsiella pneumoniae strains within hospital.
The study identifies the blaKPC-2 gene as a critical factor in the dissemination of ST11 extensively drug-resistant carbapenemase-producing Klebsiella pneumoniae strains, highlighting the role of mobile genetic elements in the spread of resistance.
Nanopore MinION Sequencing Reveals Possible Transfer of bla (KPC-2) Plasmid Across Bacterial Species in Two Healthcare Facilities.
The study identifies the transfer of a blaKPC-2-containing plasmid across three bacterial species (Citrobacter freundii, Klebsiella pneumoniae, and Escherichia coli) in two healthcare facilities, demonstrating the potential for horizontal gene transfer of carbapenem resistance.
Carbapenemase Producers Among Extensive Drug-Resistant Gram-Negative Pathogens Recovered from Febrile Neutrophilic Patients in Egypt.
The study identified blaKPC, blaOXA-48, and blaVIM as the most prevalent carbapenemase genes among XDR GNB in Egypt, with blaKPC being the most dominant.
High Colonization Rate and Heterogeneity of ESBL- and Carbapenemase-Producing Enterobacteriaceae Isolated from Gull Feces in Lisbon, Portugal.
The study identified various ESBL and carbapenemase-producing Enterobacteriaceae in gull feces, including OXA-181, OXA-48, KPC-2, KPC-3, GES-5, GES-6, and multiple CTX-M variants. These findings highlight the role of gulls as potential reservoirs for multidrug-resistant bacteria.
High Colonization Rate and Heterogeneity of ESBL- and Carbapenemase-Producing Enterobacteriaceae Isolated from Gull Feces in Lisbon, Portugal.
The study identified various ESBL and carbapenemase-producing Enterobacteriaceae in gull feces, including OXA-181, OXA-48, KPC-2, KPC-3, GES-5, GES-6, and multiple CTX-M variants. These findings highlight the role of gulls as potential reservoirs for multidrug-resistant bacteria.
Resistance to Novel β-Lactam-beta-lactamase Inhibitor Combinations: The "Price of Progress".
The paper characterizes mutations in PDC and KPC beta-lactamases that confer resistance to ceftolozane-tazobactam and ceftazidime-avibactam. Key mutations include E219K, V211A, G214R, E219G, Y221H, T96I, F121L, G156D, P154L, L293P, N346I, and others in PDC, and D179Y, D179N, R164A, R164P, D179A, D179Q, S130G, K234R, R220M, N132G, L169P, P174L, R164S, D176Y, G156R, G156D, R148H, R148P, N346Y, ΔR210-E219, ΔK204a-G222, ΔD217-Y221, ΔP208-G214, and ΔG202-E219 in KPC.
Microbiology of Meropenem-Vaborbactam: A Novel Carbapenem Beta-Lactamase Inhibitor Combination for Carbapenem-Resistant Enterobacterales Infections.
The study characterizes blaKPC-2 and blaKPC-3 as responsible for carbapenem resistance in Enterobacterales, demonstrating that meropenem-vaborbactam effectively inhibits these isolates.
Microbiology of Meropenem-Vaborbactam: A Novel Carbapenem Beta-Lactamase Inhibitor Combination for Carbapenem-Resistant Enterobacterales Infections.
The study characterizes blaKPC-2 and blaKPC-3 as responsible for carbapenem resistance in Enterobacterales, demonstrating that meropenem-vaborbactam effectively inhibits these isolates.
KPC Beta-Lactamases Are Permissive to Insertions and Deletions Conferring Substrate Spectrum Modifications and Resistance to Ceftazidime-Avibactam.
Antibacterial activity of Cinnamomum cassia L. essential oil in a carbapenem- and polymyxin-resistant Klebsiella aerogenes strain.
The study identified several AMR genes, including blaKPC-2, ampC, blaCTX-M-15, blaOXA-1, and blaTEM, in a carbapenem- and polymyxin-resistant Klebsiella aerogenes strain. Cinnamomum cassia L. essential oil showed significant antibacterial activity against this strain with a minimum inhibitory concentration of 17.57 μg/mL.
Clinical and Molecular Description of a High-Copy IncQ1 KPC-2 Plasmid Harbored by the International ST15 Klebsiella pneumoniae Clone.
The study identifies a high-copy IncQ1 plasmid carrying blaKPC-2 in ST15 Klebsiella pneumoniae isolates, highlighting its role in carbapenem resistance and potential for widespread transmission.
A Nosocomial Respiratory Infection Outbreak of Carbapenem-Resistant Escherichia coli ST131 With Multiple Transmissible bla (KPC-2) Carrying Plasmids.
The study identified blaKPC-2 as the primary carbapenem resistance gene in a nosocomial outbreak of carbapenem-resistant E. coli ST131, with multiple transmissible plasmids carrying this gene contributing to the rapid spread of resistance.
Epidemiology and Risk Factors for Carbapenem-Resistant Klebsiella Pneumoniae and Subsequent MALDI-TOF MS as a Tool to Cluster KPC-2-Producing Klebsiella Pneumoniae, a Retrospective Study.
All CRKP isolates were detected producing KPC-2 carbapenemase. blaKPC-2 is the mainstream gene of CRKP in the geographic area of analysis.
The Role of Urban Wastewater in the Environmental Transmission of Antimicrobial Resistance: The Current Situation in Italy (2010-2019).
The study identified several AMR genes in urban wastewater treatment plants in Italy, including bla TEM-1, bla AmpC, bla CTX-M-15, bla KPC-3, bla SHV-1, tet A, sul II, erm B, qnr S, int I1, aad A2, dfr 17, aadA 5, aadA 10, sat 1, bla TEM, bla OXA, bla CTX, bla KPC, and tet W. These genes confer resistance to various antibiotics such as beta-lactams, tetracyclines, sulfonamides, macrolides, fluoroquinolones, and aminoglycosides.
The Role of Urban Wastewater in the Environmental Transmission of Antimicrobial Resistance: The Current Situation in Italy (2010-2019).
The study identified several AMR genes in urban wastewater treatment plants in Italy, including bla TEM-1, bla AmpC, bla CTX-M-15, bla KPC-3, bla SHV-1, tet A, sul II, erm B, qnr S, int I1, aad A2, dfr 17, aadA 5, aadA 10, sat 1, bla TEM, bla OXA, bla CTX, bla KPC, and tet W. These genes confer resistance to various antibiotics such as beta-lactams, tetracyclines, sulfonamides, macrolides, fluoroquinolones, and aminoglycosides.
The Efficacy of AgNO3 Nanoparticles Alone and Conjugated with Imipenem for Combating Extensively Drug-Resistant Pseudomonas aeruginosa.
The study identified multiple AMR genes including IMP, VIM, OPR, SIM, SPM, GIM, NDM, VEB, PER, KPC, OXA, intI, intII, intIII, SHV, TEM, and CTXM in extensively drug-resistant Pseudomonas aeruginosa isolates. These genes conferred resistance to various antibiotics, particularly carbapenems and beta-lactams.
Interpreting k-mer–based signatures for antibiotic resistance prediction
The study identifies blaKPC as a significant resistance determinant for carbapenems in Klebsiella pneumoniae through computational and genomic analysis.
A high prevalence of multi-drug resistant Gram-negative bacilli in a Nepali tertiary care hospital and associated widespread distribution of Extended-Spectrum Beta-Lactamase (ESBL) and carbapenemase-encoding genes.
The study identified a high prevalence of multi-drug resistant Gram-negative bacilli carrying ESBL and carbapenemase genes, including bla TEM, bla CTXM-1, bla CTXM-8, bla OXA, bla OXA51, bla OXA23, bla NDM-1, and bla KPC.
KPC-53, a KPC-3 Variant of Clinical Origin Associated with Reduced Susceptibility to Ceftazidime-Avibactam.
The study identifies KPC-53, a KPC-3 variant with a Leu167Glu168 duplication in the Ω-loop, which reduces susceptibility to ceftazidime-avibactam.
KPC-53, a KPC-3 Variant of Clinical Origin Associated with Reduced Susceptibility to Ceftazidime-Avibactam.
KPC-53, a KPC-3 Variant of Clinical Origin Associated with Reduced Susceptibility to Ceftazidime-Avibactam.
High plasmid variability, and the presence of IncFIB, IncQ, IncA/C, IncHI1B, and IncL/M in clinical isolates of Klebsiella pneumoniae with bla KPC and bla NDM from patients at a public hospital in Brazil.
The study identified the presence of blaKPC and blaNDM genes in carbapenem-resistant Klebsiella pneumoniae isolates, highlighting the prevalence of these carbapenemase genes and the diversity of plasmid incompatibility groups.
Evaluation of the Xpert Carba-R NxG Assay for Detection of Carbapenemase Genes in a Global Challenge Set of Pseudomonas aeruginosa Isolates.
The Xpert Carba-R NxG assay effectively detects various carbapenemase genes, including blaVIM, blaIMP, blaNDM, blaSPM, blaKPC, and blaGES, in Pseudomonas aeruginosa isolates.
Predictors of Severity and Co-Infection Resistance Profile in COVID-19 Patients: First Report from Upper Egypt.
The study identified various antimicrobial resistance genes in bacterial co-infections among COVID-19 patients, including mecA, NDM-1, KPC, TEM, CTX-M, and SHV. These genes were associated with multidrug-resistant isolates, highlighting the importance of monitoring and managing co-infections in COVID-19 patients.
A Novel KPC Variant KPC-55 in Klebsiella pneumoniae ST307 of Reinforced Meropenem-Hydrolyzing Activity.
The study identifies a novel KPC variant, KPC-55, which exhibits increased hydrolytic activity towards meropenem and aztreonam compared to KPC-2, despite the Y264N amino acid substitution being located outside the active site.
A Novel KPC Variant KPC-55 in Klebsiella pneumoniae ST307 of Reinforced Meropenem-Hydrolyzing Activity.
A Novel KPC Variant KPC-55 in Klebsiella pneumoniae ST307 of Reinforced Meropenem-Hydrolyzing Activity.
Carbapenemase-Producing Gram-Negative Bacteria from American Crows in the United States.
The study identified carbapenemase-producing Gram-negative bacteria in American crows, including Providencia rettgeri with blaIMP-27, Klebsiella pneumoniae with blaKPC-2, and Enterobacter bugandensis with blaIMI-1.
Antibacterial Activity of a Cationic Antimicrobial Peptide against Multidrug-Resistant Gram-Negative Clinical Isolates and Their Potential Molecular Targets.
The study identifies the presence of carbapenemase genes bla KPC, bla NDM, bla VIM, and bla IMP in multidrug-resistant clinical isolates of Klebsiella pneumoniae and Pseudomonas aeruginosa, which confer resistance to carbapenems.
Antibiogram and Genetic Characterization of Carbapenem-Resistant Gram-Negative Pathogens Incriminated in Healthcare-Associated Infections.
The study identified bla KPC and bla GES genes as the primary contributors to carbapenem resistance in Gram-negative pathogens, with high prevalence among the tested isolates.
Virulence-determinants and antibiotic-resistance genes of MDR-E. coli isolated from secondary infections following FMD-outbreak in cattle.
The study identified the presence of multidrug-resistant E. coli strains carrying the blaTEM, blaCTX, and blaKPC genes, which confer resistance to penicillins, cephalosporins, and carbapenems, respectively.
In vitro Synergistic Activity of Antimicrobial Combinations Against bla (KPC) and bla (NDM)-Producing Enterobacterales With bla (IMP) or mcr Genes.
The study evaluated the synergistic effects of various antimicrobial combinations against carbapenemase-producing Enterobacterales, identifying bla KPC, bla NDM, bla IMP, and mcr genes as critical resistance determinants.
Can the use of older-generation beta-lactam antibiotics in livestock production over-select for beta-lactamases of greatest consequence for human medicine? An in vitro experimental model.
The study investigated the selection of beta-lactamases in E. coli strains under different beta-lactam antibiotics, identifying bla TEM-1, bla CMY-2, bla CTX-M-*, and bla KPC/IMP/NDM as conferring resistance to various beta-lactam antibiotics.
Multiplicity of Carbapenemase-Producers Three Years after a KPC-3-Producing K. pneumoniae ST147-K64 Hospital Outbreak.
The study identifies the prevalence of various carbapenemase-producing Enterobacterales, including KPC-3, OXA-48, and VIM-1, highlighting the diversity and persistence of these resistant strains in a hospital setting.
Extended Spectrum Beta-Lactamase-Resistant Determinants among Carbapenem-Resistant Enterobacteriaceae from Beef Cattle in the North West Province, South Africa: A Critical Assessment of Their Possible Public Health Implications.
The study identified various carbapenemase and ESBL genes in carbapenem-resistant Enterobacteriaceae isolated from beef cattle in South Africa, highlighting the presence of resistance determinants that could pose public health risks.
Can We Exploit beta-lactamases Intrinsic Dynamics for Designing More Effective Inhibitors?
The study explores the flexibility of beta-lactamases and identifies key residues and structural features that influence their catalytic efficiency and resistance mechanisms. It highlights the importance of allosteric sites for designing more effective inhibitors.
Clinical Observation and Prognostic Analysis of Patients With Klebsiella pneumoniae Bloodstream Infection.
The study highlights the poor prognosis of Klebsiella pneumoniae bloodstream infections, particularly with carbapenem-resistant strains. It identifies risk factors for drug-resistant bacterial infection and mortality, emphasizing the importance of early identification and appropriate antibiotic treatment.
KPC-2 beta-lactamase enables carbapenem antibiotic resistance through fast deacylation of the covalent intermediate.
KPC-2 beta-lactamase confers resistance to carbapenems through fast deacylation of the covalent intermediate. Mutations at positions 170, 220, 235, and 237 affect the enzyme's ability to hydrolyze β-lactam antibiotics.
Co-occurrence of Rapid Gene Gain and Loss in an Interhospital Outbreak of Carbapenem-Resistant Hypervirulent ST11-K64 Klebsiella pneumoniae.
The study identifies the carbapenemase gene bla KPC–2 and the aminoglycoside modifying gene rmtB as key contributors to carbapenem and aminoglycoside resistance in ST11-K64 Klebsiella pneumoniae strains during an interhospital outbreak. Additionally, the loss of the 10-kbp fragment containing rmtB led to reduced aminoglycoside resistance.
Emergence of a Clinical Escherichia coli Sequence Type 131 Strain Carrying a Chromosomal bla (KPC-2) Gene.
The study reports the first clinical strain of E. coli ST131 carrying a chromosomal blaKPC-2 gene, which was horizontally transferred from a P. mirabilis plasmid via the IS26 element.
Occurrence, identification, and antibiogram signatures of selected Enterobacteriaceae from Tsomo and Tyhume rivers in the Eastern Cape Province, Republic of South Africa.
The study identified various beta-lactamase genes (bla TEM, bla CTX-M, bla SHV, bla OXA-1-like, bla PER, bla VIM, bla IMP, bla KPC, bla GES, bla OXA-48-like), plasmid-mediated AmpC beta-lactamase genes (bla EBC, bla ACC, bla FOX, bla CIT), tetracycline resistance genes (tetA, tetB, tetD, tetM), chloramphenicol resistance gene (catII), and sulfonamide resistance gene (sulII) in Enterobacteriaceae isolates from Tsomo and Tyhume rivers.
First Insights into Clinical and Resistance Features of Infections by Klebsiella pneumoniae among Oncological Patients from a Referral Center in Amazon Region, Brazil.
The study identified the presence of bla KPC-1 gene in seven K. pneumoniae isolates, indicating carbapenem resistance. Additionally, two colistin-resistant isolates were found, highlighting the emergence of resistance to polymyxins.
The Novel CarbaLux Test for Carbapenemases and Carbapenem Deactivating AmpC Beta-Lactamases.
The CarbaLux test was developed to rapidly detect carbapenemase-producing bacteria, including various OXA-type carbapenemases (OXA-23, OXA-24/40, OXA-48, OXA-181) and other carbapenemases (KPC, NDM, VIM, IMP-1, OXA-58). It also detects hyper-produced AmpC beta-lactamases, which deactivate carbapenems but are not detectable by previous rapid phenotypic assays.
Convergence of carbapenem resistance and hypervirulence in a highly-transmissible ST11 clone of K. pneumoniae: An epidemiological, genomic and functional study.
The study identifies blaKPC-2 as a key carbapenem resistance gene and rmpA2 as a virulence factor in ST11 K. pneumoniae clones, highlighting the convergence of hypervirulence and carbapenem resistance.
Biofilm Production by Carbapenem-Resistant Klebsiella pneumoniae Significantly Increases the Risk of Death in Oncological Patients.
The study identifies blaKPC, blaOXA-48, and blaVIM as the primary carbapenem resistance genes in CRKP isolates, with blaKPC being the most prevalent. Strong biofilm-producing CRKP strains were associated with increased mortality in oncological patients.
Occurrence of KPC-Producing Escherichia coli in Psittaciformes Rescued from Trafficking in Paraíba, Brazil.
The study reports the occurrence of KPC-producing Escherichia coli in Psittaciformes rescued from wildlife trafficking in Brazil, highlighting the potential role of these birds as reservoirs of antimicrobial resistance.
Carriage of Carbapenem-Resistant Enterobacterales in Adult Patients Admitted to a University Hospital in Italy.
The study identified the blaKPC gene as the primary carbapenem resistance gene in carbapenem-resistant Enterobacterales (CRE) isolates, with the exception of one E. coli strain. The blaKPC gene was found in all isolates except for one E. coli strain, and it was often combined with blaVIM in a single K. pneumoniae strain.
Emergence of the New KPC-49 Variant Conferring an ESBL Phenotype with Resistance to Ceftazidime-Avibactam in the ST131-H30R1 Escherichia coli High-Risk Clone.
The study identifies a novel KPC-49 variant, an Arg-163-Ser mutant of bla KPC-3, which confers resistance to ceftazidime-avibactam in the ST131-H30R1 E. coli high-risk clone.
Emergence of the New KPC-49 Variant Conferring an ESBL Phenotype with Resistance to Ceftazidime-Avibactam in the ST131-H30R1 Escherichia coli High-Risk Clone.
Emergence of the New KPC-49 Variant Conferring an ESBL Phenotype with Resistance to Ceftazidime-Avibactam in the ST131-H30R1 Escherichia coli High-Risk Clone.
Detection of IncN-pST15 one-health plasmid harbouring bla(KPC-2) in a hypermucoviscous Klebsiella pneumoniae CG258 isolated from an infected dog, Brazil.
The study identifies a hypermucoviscous KPC-2-producing Klebsiella pneumoniae strain isolated from a dog, which harbors the bla(KPC-2) gene on an IncN-pST15 plasmid, indicating the potential for cross-transmission of carbapenem-resistant bacteria between humans and pets.
Distribution of Beta-Lactamase Producing Gram-Negative Bacterial Isolates in Isabela River of Santo Domingo, Dominican Republic.
The study identified several beta-lactamase genes, including bla TEM, bla OXA, bla SHV, and bla KPC, as well as other resistance genes such as CTX-M-15, CTX-M-55, OXA-1, OXA-72, OXA-132, KPC-3, QnrS1, QnrB19, sul2, sul1, dfrA12, dfrA14, dfrA17, mphA, aadA, aadA2, aadA5, ampC, ampC1, ampH, PmrF, bacA, and eptA, in various Gram-negative bacterial isolates from the Isabela River in the Dominican Republic.
Distribution of Beta-Lactamase Producing Gram-Negative Bacterial Isolates in Isabela River of Santo Domingo, Dominican Republic.
The study identified several beta-lactamase genes, including bla TEM, bla OXA, bla SHV, and bla KPC, as well as other resistance genes such as CTX-M-15, CTX-M-55, OXA-1, OXA-72, OXA-132, KPC-3, QnrS1, QnrB19, sul2, sul1, dfrA12, dfrA14, dfrA17, mphA, aadA, aadA2, aadA5, ampC, ampC1, ampH, PmrF, bacA, and eptA, in various Gram-negative bacterial isolates from the Isabela River in the Dominican Republic.
Molecular Epidemiology and Characterization of Carbapenem-Resistant Klebsiella pneumoniae Isolated from Urine at a Teaching Hospital in Taiwan.
The study identified several AMR genes and mutations contributing to carbapenem resistance in K. pneumoniae isolates from urine, including bla TEM, bla SHV, bla CTX-M, bla DHA, bla KPC, bla VIM, and mutations in ompK35 and ompK36.
Analysis of Antibiotic Resistance and Virulence Traits (Genetic and Phenotypic) in Klebsiella pneumoniae Clinical Isolates from Pakistan: Identification of Significant Levels of Carbapenem and Colistin Resistance.
The study identifies bla NDM-1, bla OXA-48, and bla KPC as the most prevalent carbapenemase genes in K. pneumoniae isolates from Pakistan, along with mcr-1 and mcr-2 contributing to colistin resistance. Additionally, the rmpA gene was found to be associated with the hypermucoviscous phenotype.
Distribution of beta-lactamase Genes and Genetic Context of bla (KPC-2) in Clinical Carbapenemase-Producing Klebsiella pneumoniae Isolates.
The study identified the bla KPC-2 gene as a significant contributor to carbapenem resistance in Klebsiella pneumoniae isolates, highlighting its presence in diverse genetic contexts and its role in multidrug resistance.
A Polyclonal Spread Emerged: Characteristics of Carbapenem-Resistant Klebsiella pneumoniae Isolates from the Intensive Care Unit in a Chinese Tertiary Hospital.
The study identified multiple carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates carrying bla KPC-2, bla NDM-5, and bla NDM-1 genes, highlighting the diversity of resistance mechanisms in the ICU setting.
Molecular characterization of KPC-2-producing Klebsiella pneumoniae ST258 isolated from bovine mastitis.
The study identifies the KPC-2-producing Klebsiella pneumoniae ST258 strain isolated from bovine mastitis, highlighting the presence of the blaKPC-2 gene responsible for carbapenem resistance.
KPC-12 with a L169M substitution in the Ω loop has reduced carbapenemase activity.
KPC-12 with a L169M substitution in the Ω loop has reduced carbapenemase activity.
KPC-12 with a L169M substitution in the Ω loop has reduced carbapenemase activity.
In vitro and in vivo antibacterial activity assays of carvacrol: A candidate for development of innovative treatments against KPC-producing Klebsiella pneumoniae.
The study identified several AMR genes, including bla KPC-2, bla OXA-48, bla NDM-1, bla CTX-M-8, and alterations in the mgr B gene, which contribute to carbapenem and polymyxin resistance in K. pneumoniae strains.
Faropenem reacts with serine and metallo-beta-lactamases to give multiple products.
The study characterizes the reactions of faropenem with beta-lactamases, including KPC-2, VIM-2, and L1, revealing distinct product formations and highlighting the importance of enzyme-specific interactions in determining the fate of faropenem.
Clinical and microbiological characteristics of nosocomial, healthcare-associated, and community-acquired Klebsiella pneumoniae infections in Guangzhou, China.
The study identified bla KPC, bla NDM, and bla OXA-48-like as carbapenemase genes, and bla SHV, bla TEM, bla CTX-M-9-group, and bla CTX-M-1-group as ESBL genes in Klebsiella pneumoniae isolates. CRKP and ESBL-producing strains were predominantly found in healthcare-associated and nosocomial infections.
Polyphasic characterization of carbapenem-resistant Klebsiella pneumoniae clinical isolates suggests vertical transmission of the blaKPC-3 gene.
The study identifies the blaKPC-3 gene as a key determinant of carbapenem resistance in Klebsiella pneumoniae clinical isolates, showing its association with specific plasmid types and confirming its vertical transmission within a healthcare facility.
Multinational evaluation of the BioFire® FilmArray® Pneumonia plus Panel as compared to standard of care testing.
The BioFire® FilmArray® Pneumonia plus Panel outperformed standard of care testing in detecting pathogens and antibiotic resistance markers, including bla CTX-M, bla KPC, bla VIM, bla OXA-48-like, and bla IMP genes.
Genomic data reveals the emergence of an IncQ1 small plasmid carrying bla(KPC-2) in Escherichia coli of the pandemic sequence type 648.
The study identifies an IncQ1 plasmid carrying the bla(KPC-2) gene in a pandemic Escherichia coli ST648 strain, highlighting the emergence of this plasmid as a carrier of carbapenem resistance.
Evaluation of loop-mediated isothermal amplification assays for rapid detection of blaKPC producing Serratia spp. in clinical specimens: A prospective diagnostic accuracy study.
The study developed a LAMP assay for rapid detection of blaKPC-producing Serratia spp. with high sensitivity and specificity.
Identification of a Novel Hybrid Plasmid Encoding KPC-2 and Virulence Factors in Klebsiella pneumoniae Sequence Type 11.
The study identifies a novel hybrid plasmid, pCRHV-C2244, in a carbapenem-resistant Klebsiella pneumoniae ST11 isolate, which encodes the beta-lactamase KPC-2 along with various virulence factors. The plasmid shows stable maintenance without significant fitness costs and enhances virulence in vitro and in vivo.
Prevalence of pathogenic Klebsiella pneumoniae based on PCR capsular typing harbouring carbapenemases encoding genes in Uganda tertiary hospitals.
The study identified various carbapenemase-encoding genes including bla_OXA-48-like, bla_VIM, bla_IMP, bla_KPC, and bla_NDM in Klebsiella pneumoniae isolates from Ugandan tertiary hospitals, highlighting the prevalence of carbapenem resistance.
Market Chickens as a Source of Antibiotic-Resistant Escherichia coli in a Peri-Urban Community in Lima, Peru.
The study identified several AMR genes in E. coli isolates from market chickens and humans, including blaCTX-M-55, blaKPC-3, mcr-1, and floR, highlighting the potential transmission of resistance genes from poultry to humans.
Molecular characterization of carbapenem-resistant and virulent plasmids in Klebsiella pneumoniae from patients with bloodstream infections in China.
The study identifies the role of IS Kpn26 insertion in the acrR gene leading to multidrug resistance in ST11- bla KPC-2 Klebsiella pneumoniae. It also characterizes novel MDR-virulent plasmids carrying virulence factors and multiple antibiotic resistance genes.
Methylation analysis of Klebsiella pneumoniae from Portuguese hospitals.
The study identifies bla CTX-M-15 and bla KPC-3 as key resistance genes in Klebsiella pneumoniae isolates from Portuguese hospitals, along with fosA contributing to fosfomycin resistance. Methylation analysis reveals unique patterns around these genes.
Antimicrobial Susceptibility Profiles To Predict the Presence of Carbapenemase Genes among Carbapenem-Resistant Pseudomonas aeruginosa Isolates.
The study identifies bla VIM, bla KPC, bla IMP, and bla NDM as carbapenemase genes associated with carbapenem-resistant Pseudomonas aeruginosa (CRPA) isolates. These genes were detected in CRPA isolates and were linked to reduced susceptibility to carbapenems.
Molecular analysis of clinical isolates of ceftazidime-avibactam-resistant Klebsiella pneumoniae
The study identified various mutations in the blaKPC gene among ceftazidime-avibactam-resistant KPC-producing K. pneumoniae strains, along with several beta-lactamase genes contributing to resistance.
Molecular analysis of clinical isolates of ceftazidime-avibactam-resistant Klebsiella pneumoniae
The study identified various mutations in the blaKPC gene among ceftazidime-avibactam-resistant KPC-producing K. pneumoniae strains, along with several beta-lactamase genes contributing to resistance.
Molecular analysis of clinical isolates of ceftazidime-avibactam-resistant Klebsiella pneumoniae
The study identified various mutations in the blaKPC gene among ceftazidime-avibactam-resistant KPC-producing K. pneumoniae strains, along with several beta-lactamase genes contributing to resistance.
Molecular analysis of clinical isolates of ceftazidime-avibactam-resistant Klebsiella pneumoniae
The study identified various mutations in the blaKPC gene among ceftazidime-avibactam-resistant KPC-producing K. pneumoniae strains, along with several beta-lactamase genes contributing to resistance.
Molecular analysis of clinical isolates of ceftazidime-avibactam-resistant Klebsiella pneumoniae.
Molecular analysis of clinical isolates of ceftazidime-avibactam-resistant Klebsiella pneumoniae.
Molecular analysis of clinical isolates of ceftazidime-avibactam-resistant Klebsiella pneumoniae.
Molecular analysis of clinical isolates of ceftazidime-avibactam-resistant Klebsiella pneumoniae.
Molecular analysis of clinical isolates of ceftazidime-avibactam-resistant Klebsiella pneumoniae.
Molecular analysis of clinical isolates of ceftazidime-avibactam-resistant Klebsiella pneumoniae.
Molecular analysis of clinical isolates of ceftazidime-avibactam-resistant Klebsiella pneumoniae.
Molecular analysis of clinical isolates of ceftazidime-avibactam-resistant Klebsiella pneumoniae.
Prevalence of Carbapenem-Resistant Klebsiella pneumoniae Co-Harboring blaKPC-Carrying Plasmid and pLVPK-Like Virulence Plasmid in Bloodstream Infections.
The study characterizes carbapenem-resistant Klebsiella pneumoniae co-harboring blaKPC-2-carrying plasmid and pLVPK-like virulence plasmid, highlighting their multidrug resistance, enhanced virulence, and transferability.
Characterization of antimicrobial-resistant Gram-negative bacteria that cause neonatal sepsis in seven low- and middle-income countries.
The study identifies various AMR genes, including bla CTX-M-15, bla NDM-1, bla NDM-5, bla NDM-7, bla OXA-181, bla OXA-232, bla VIM-2, and bla VIM-6, which confer resistance to multiple antibiotics in Gram-negative bacteria causing neonatal sepsis in low- and middle-income countries.
The inhibitory effects of Staphylococcus aureus on the antibiotic susceptibility and virulence factors of Pseudomonas aeruginosa: A549 cell line model.
The study found that the KPC gene expression level remarkably decreased in PA-2 and PA-3 strains of Pseudomonas aeruginosa during co-culture with Staphylococcus aureus, leading to reduced carbapenem resistance.
An Update on Wastewater Multi-Resistant Bacteria: Identification of Clinical Pathogens Such as Escherichia coli O25b:H4-B2-ST131-Producing CTX-M-15 ESBL and KPC-3 Carbapenemase-Producing Klebsiella oxytoca.
The study identifies several AMR genes, including bla CTX-M-15, bla KPC-3, aac(6')-Ib-cr5, and various dfr and aad genes, in wastewater isolates, highlighting the prevalence of multidrug-resistant bacteria in wastewater treatment plants.
Acinetobacter baumannii Antibiotic Resistance Mechanisms.
The paper reviews various beta-lactamases and other resistance mechanisms in Acinetobacter baumannii, focusing on their roles in resistance to beta-lactams, aminoglycosides, and other antibiotics.
Prevalence of bla(KPC-2), bla(KPC-3) and bla(KPC-30)-Carrying Plasmids in Klebsiella pneumoniae Isolated in a Brazilian Hospital.
Prevalence of bla(KPC-2), bla(KPC-3) and bla(KPC-30)-Carrying Plasmids in Klebsiella pneumoniae Isolated in a Brazilian Hospital.
Prevalence of bla(KPC-2), bla(KPC-3) and bla(KPC-30)-Carrying Plasmids in Klebsiella pneumoniae Isolated in a Brazilian Hospital.
Clonal Dissemination of KPC-2, VIM-1, OXA-48-Producing Klebsiella pneumoniae ST147 in Katowice, Poland.
The study identified the presence of bla KPC-2, bla OXA-48, bla VIM-1, and bla CTX-M-15 genes in all 15 K. pneumoniae isolates, indicating resistance to carbapenems and other beta-lactam antibiotics.
Multicentre study of the burden of multidrug-resistant bacteria in the aetiology of infected diabetic foot ulcers.
The study identified various AMR genes including bla CTX-M, bla TEM, bla SHV, mecA, bla VIM, bla KPC, and bla NDM in multidrug-resistant bacteria causing infected diabetic foot ulcers. These genes conferred resistance to beta-lactams, carbapenems, and other antibiotics.
A multispecies outbreak of carbapenem-resistant bacteria harboring the bla(KPC) gene in a non-classical transposon element.
The study identified the bla(KPC) gene in a novel non-Tn4401 transposon element (NTE KPC -IIe) in a multispecies outbreak of carbapenem-resistant bacteria, highlighting horizontal gene transfer as a major driver of dissemination.
Comprehensive Pathogen Identification, Antibiotic Resistance, and Virulence Genes Prediction Directly From Simulated Blood Samples and Positive Blood Cultures by Nanopore Metagenomic Sequencing.
The study demonstrates the use of nanopore sequencing for rapid identification of pathogens, antibiotic resistance genes, and virulence genes from simulated blood samples and positive blood cultures. It identified 39 antibiotic resistance genes and 77 virulence genes in a Klebsiella pneumoniae strain, including blaKPC-2, blaSHV-12, blaTEM-1, blaCTX-M-65, rmtB, aadA, AAC(6')-IIb, baeR, mdtABC, acrAB, oqxAB, tet, H-NS, gyrA, and parC.
Screening of Antimicrobial Resistance Genes and Epidemiological Features in Hospital and Community-Associated Carbapenem-Resistant Pseudomonas aeruginosa Infections.
The study identified several carbapenem resistance genes, including blaVEB, blaPER, blaNDM, blaKPC, blaIMP, blaVIM, blaOXA-48, and blaOXA-23, in carbapenem-resistant Pseudomonas aeruginosa isolates.
Two Acinetobacter baumannii Isolates Obtained From a Fatal Necrotizing Fasciitis Infection Display Distinct Genomic and Phenotypic Characteristics in Comparison to Type Strains.
The study characterizes two XDR Acinetobacter baumannii isolates, NFAb-1 and NFAb-2, obtained from a fatal necrotizing fasciitis case. These isolates exhibit resistance to multiple antibiotics, including carbapenems, aminoglycosides, fluoroquinolones, and others, indicating the presence of various AMR genes such as blaOXA-51-like, blaOXA-23-like, blaOXA-58-like, blaADC, blaNDM, blaKPC, aac(6')-Ib, aadA, ant(3'')-Ia, mph(A), erm(B), tet(A), tet(G), qnrS1, mexAB-oprM, acrAB-tolC, oqxAB, cat, cfr, and optrA.
The Direct Semi-Quantitative Detection of 18 Pathogens and Simultaneous Screening for Nine Resistance Genes in Clinical Urine Samples by a High-Throughput Multiplex Genetic Detection System.
The study presents a high-throughput multiplex genetic detection system (UTI-HMGS) capable of semi-quantitative detection of 18 uropathogens and simultaneous screening for nine antibiotic resistance genes directly from clinical urine samples within 4 hours. The system demonstrated high sensitivity and specificity for the detection of uropathogens and resistance genes, with improved detection rates for several important uropathogens compared to conventional culture methods.
Cross-resistance to cefiderocol and ceftazidime-avibactam in KPC beta-lactamase mutants and the inoculum effect
The study identified KPC beta-lactamase mutants that exhibit cross-resistance to cefiderocol and ceftazidime-avibactam, with certain mutations significantly increasing the MIC values for these antibiotics in E. coli TOP10.
Cross-resistance to cefiderocol and ceftazidime-avibactam in KPC beta-lactamase mutants and the inoculum effect
The study identified KPC beta-lactamase mutants that exhibit cross-resistance to cefiderocol and ceftazidime-avibactam, with certain mutations significantly increasing the MIC values for these antibiotics in E. coli TOP10.
Cross-resistance to cefiderocol and ceftazidime-avibactam in KPC β-lactamase mutants and the inoculum effect.
Cross-resistance to cefiderocol and ceftazidime-avibactam in KPC β-lactamase mutants and the inoculum effect.
Cross-resistance to cefiderocol and ceftazidime-avibactam in KPC β-lactamase mutants and the inoculum effect.
Galactose-Clicked Curcumin-Mediated Reversal of Meropenem Resistance among Klebsiella pneumoniae by Targeting Its Carbapenemases and the AcrAB-TolC Efflux System.
The study identified the bla_KPC gene as a key contributor to meropenem resistance in Klebsiella pneumoniae and demonstrated that curcumin derivatives could reverse this resistance by inhibiting carbapenemases and the AcrAB-TolC efflux system.
Combining Functional Genomics and Whole-Genome Sequencing to Detect Antibiotic Resistance Genes in Bacterial Strains Co-Occurring Simultaneously in a Brazilian Hospital.
The study identified multiple beta-lactamase genes, including blaTEM-105, blaOXA-1, blaKPC-2, blaCTX-M-15, blaCMY-111, blaOXA-10, blaCTX-M-1, blaHYDRO, and blaBLP, along with qnrS1, aadC, and ermC, which confer resistance to beta-lactam, quinolone, aminoglycoside, and macrolide/lincosamide/streptogramin B antibiotics. These genes were found in various bacterial species and were validated through functional screening.
Combining Functional Genomics and Whole-Genome Sequencing to Detect Antibiotic Resistance Genes in Bacterial Strains Co-Occurring Simultaneously in a Brazilian Hospital.
Combining Functional Genomics and Whole-Genome Sequencing to Detect Antibiotic Resistance Genes in Bacterial Strains Co-Occurring Simultaneously in a Brazilian Hospital.
Combining Functional Genomics and Whole-Genome Sequencing to Detect Antibiotic Resistance Genes in Bacterial Strains Co-Occurring Simultaneously in a Brazilian Hospital.
Combining Functional Genomics and Whole-Genome Sequencing to Detect Antibiotic Resistance Genes in Bacterial Strains Co-Occurring Simultaneously in a Brazilian Hospital.
Risk Factors for Mortality in Adult COVID-19 Patients Who Develop Bloodstream Infections Mostly Caused by Antimicrobial-Resistant Organisms: Analysis at a Large Teaching Hospital in Italy.
The study identified several antimicrobial resistance genes, including mecA, bla CTX-M-15, bla KPC, vanA, bla OXA-23, and a mutated FKS gene, in various pathogens causing bloodstream infections in COVID-19 patients.
Evaluation of Xpert Carba-R Assay for the Detection of Carbapenemase Genes in Gram-Negative Bacteria.
The Xpert Carba-R assay demonstrates 100% precision in identifying carbapenemase genes in Gram-negative bacteria, making it an effective tool for early clinical detection.
Multimodal Interventions to Prevent and Control Carbapenem-Resistant Enterobacteriaceae and Extended-Spectrum β-Lactamase Producer-Associated Infections at a Tertiary Care Hospital in Egypt.
The study identified several carbapenemase genes (bla KPC, bla NDM, bla VIM, bla OXA-48, bla IMP) and extended-spectrum β-lactamase genes (bla CTX-m, bla TEM, bla SHV) in CRE and ESBL-producing isolates, highlighting the prevalence of these resistance mechanisms in a tertiary care hospital in Egypt.
atpD gene sequencing, multidrug resistance traits, virulence-determinants, and antimicrobial resistance genes of emerging XDR and MDR-Proteus mirabilis.
The study identified multiple antimicrobial resistance genes in XDR and MDR-Proteus mirabilis isolates from ducks, including blaTEM, blaOXA-1, blaCTX-M, tetA, sul1, blaNDM-1, and blaKPC. These genes confer resistance to various antibiotic classes such as penicillins, cephalosporins, tetracyclines, sulfonamides, and carbapenems.
In vitro activity of imipenem/relebactam, meropenem/vaborbactam, ceftazidime/avibactam, cefepime/zidebactam and other novel antibiotics against imipenem-non-susceptible Gram-negative bacilli from Taiwan.
The study evaluated the in vitro activity of several novel antibiotics against imipenem-non-susceptible Gram-negative bacilli, highlighting the effectiveness of β-lactam/BLI-BLE combinations against isolates with various carbapenemase genes.
Characterization of KPC-82, a KPC-2 Variant Conferring Resistance to Ceftazidime-Avibactam in a Carbapenem-Nonsusceptible Clinical Isolate of Citrobacter koseri.
The study identifies KPC-82, a KPC-2 variant that confers high-level resistance to ceftazidime-avibactam in a carbapenem-nonsusceptible Citrobacter koseri isolate. The resistance is attributed to a two-amino-acid insertion (Ser-Asp) in the KPC protein, resulting from a 6-nucleotide tandem repeat duplication.
Characterization of KPC-82, a KPC-2 Variant Conferring Resistance to Ceftazidime-Avibactam in a Carbapenem-Nonsusceptible Clinical Isolate of Citrobacter koseri.
Characterization of KPC-82, a KPC-2 Variant Conferring Resistance to Ceftazidime-Avibactam in a Carbapenem-Nonsusceptible Clinical Isolate of Citrobacter koseri.
Epidemiology and Transmission of Carbapenemase-Producing Enterobacteriaceae in a Health Care Network of an Acute-Care Hospital and Its Affiliated Intermediate- and Long-Term-Care Facilities in Singapore.
The study identified several carbapenemase genes, including bla_IMI, bla_NDM-1, and bla_KPC-2, in Enterobacteriaceae isolates from an acute-care hospital and its affiliated intermediate- and long-term-care facilities in Singapore. These genes were associated with carbapenem resistance and were found in various bacterial species such as Enterobacter cloacae and Escherichia coli.
Emergence and Expansion of a Carbapenem-Resistant Pseudomonas aeruginosa Clone Are Associated with Plasmid-Borne bla (KPC-2) and Virulence-Related Genes.
The study identifies blaKPC-2 as a plasmid-borne carbapenem resistance gene associated with the expansion of a Pseudomonas aeruginosa clone (ST463).
Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Sequence Type 11 Due to a Mutation in Plasmid-Borne bla (kpc-2) to bla (kpc-33), in Henan, China.
The study identifies a mutation in the bla(KPC-2) gene leading to the emergence of bla(KPC-33) in Klebsiella pneumoniae ST11, causing resistance to ceftazidime-avibactam.
Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Sequence Type 11 Due to a Mutation in Plasmid-Borne bla (kpc-2) to bla (kpc-33), in Henan, China.
The study identifies a mutation in the bla(KPC-2) gene leading to the emergence of bla(KPC-33) in Klebsiella pneumoniae ST11, causing resistance to ceftazidime-avibactam.
Isolation Procedure for CP E. coli from Caeca Samples under Review towards an Increased Sensitivity.
The study characterizes several carbapenemase genes (bla VIM-1, bla GES-5, bla KPC-2, bla NDM-1, and bla OXA-48) in CP E. coli isolates from caeca samples, highlighting their role in carbapenem resistance and evaluating improved isolation methods for better detection.
Clinical evolution of ST11 carbapenem resistant and hypervirulent Klebsiella pneumoniae.
The study identifies a conjugative plasmid p17ZR-91-Vir-KPC that encodes both carbapenem resistance and hypervirulence in Klebsiella pneumoniae, providing insight into the evolution of ST11 carbapenem-resistant and hypervirulent strains.
A Comparison of Blood Pathogen Detection Among Droplet Digital PCR, Metagenomic Next-Generation Sequencing, and Blood Culture in Critically Ill Patients With Suspected Bloodstream Infections.
The study identified bla KPC and mecA genes using ddPCR in blood samples from critically ill patients with suspected bloodstream infections, demonstrating the effectiveness of ddPCR for rapid detection of antimicrobial resistance genes.
Molecular Epidemiology and Drug Resistant Mechanism of Carbapenem-Resistant Klebsiella pneumoniae in Elderly Patients With Lower Respiratory Tract Infection.
The study identified blaKPC-2 and blaNDM-5 as the main carbapenem resistance genes in carbapenem-resistant Klebsiella pneumoniae (CRKp) isolates from elderly patients with lower respiratory tract infections. blaKPC-2 was the most prevalent, while blaNDM-5 was found in two isolates.
Polymyxin B Pharmacodynamics in the Hollow-Fiber Infection Model: What You See May Not Be What You Get.
The study investigated the pharmacodynamics of polymyxin B (PMB) in a hollow-fiber infection model, identifying mechanisms of resistance development in Pseudomonas aeruginosa and Klebsiella pneumoniae, including transient lower-level adaptive resistance, higher-level stable resistance, and moderately stable adaptive resistance.
Detection of carbapenemase producing enterobacteria using an ion sensitive field effect transistor sensor.
The study evaluated the effectiveness of an ion-sensitive field-effect transistor (ISFET) sensor for detecting carbapenemase-producing enterobacteria. It successfully identified various carbapenemase genes including blaNDM-1, blaVIM-1, blaIMP-1, blaKPC-2, blaNMC-A, and blaOXA-48 in different bacterial strains.
Risk factors for the development of neonatal sepsis in a neonatal intensive care unit of a tertiary care hospital of Nepal.
The study identified bla TEM and bla KPC genes as the most common ESBL and carbapenemase genes in Gram-negative bacilli causing neonatal sepsis in a Nepalese NICU.
Molecular Characteristics of Escherichia coli Causing Bloodstream Infections During 2010-2015 in a Tertiary Hospital, Shanghai, China.
The study identified bla KPC as a gene conferring carbapenem resistance in E. coli, although it was rarely detected. The low expression of bla KPC in E. coli A59 was associated with reduced resistance to carbapenems.
One-Step Detection and Classification of Bacterial Carbapenemases in 10 Minutes Using Fluorescence Identification of β-Lactamase Activity.
The study presents a novel assay called FIBA for the rapid detection and classification of bacterial carbapenemases. It identifies various carbapenemase types including KPC, SME, NMC-A, NDM, VIM, IMP, and OXA, demonstrating high sensitivity and specificity.
A Sequence Type 23 Hypervirulent Klebsiella pneumoniae Strain Presenting Carbapenem Resistance by Acquiring an IncP1 bla(KPC-2) Plasmid.
The study identifies a carbapenem-resistant hypervirulent Klebsiella pneumoniae strain, ZJ27003, which carries the IncP1 bla(KPC-2) plasmid, enabling resistance to carbapenems and facilitating the transfer of this resistance to Pseudomonas aeruginosa.
Carbapenem-resistant Enterobacter cloacae complex in a tertiary Hospital in Northeast China, 2010-2019.
The study identified several carbapenemase-encoding genes, including blaNDM-1, blaNDM-5, blaIMP-4, and blaKPC-2, along with various beta-lactamase genes such as TEM-1, CTX-M-15, CTX-M-3, SHV-12, and CTX-M-14, in carbapenem-resistant Enterobacter cloacae complex isolates.
Molecular Characterization of KPC-2-Producing Enterobacter cloacae Complex Isolates from Cali, Colombia.
The study identified the bla KPC-2 gene as a significant contributor to carbapenem resistance in Enterobacter cloacae complex isolates from Cali, Colombia.
The Beta-Lactam Resistome Expressed by Aerobic and Anaerobic Bacteria Isolated from Human Feces of Healthy Donors.
The study identifies several beta-lactamase genes, including blaTEM, blaSHV, blaKPC, blaCMY, blaBIL, blaCFX, and blaP, in aerobic and anaerobic bacteria isolated from the feces of healthy donors, highlighting the human gut as a reservoir of beta-lactam resistance.
Bacterial characteristics of carbapenem-resistant Enterobacteriaceae (CRE) colonized strains and their correlation with subsequent infection.
The study identified bla KPC-2 and bla NDM as the primary carbapenemase-encoding genes in CRE strains, with KPC-CRE being a significant risk factor for subsequent CRE infection.
KPC-Mediated Resistance to Ceftazidime-Avibactam and Collateral Effects in Klebsiella pneumoniae.
KPC-Mediated Resistance to Ceftazidime-Avibactam and Collateral Effects in Klebsiella pneumoniae.
Analysis of the molecular characteristics of a blaKPC-2-harbouring untypeable plasmid in Serratia marcescens.
The study identified a blaKPC-2-harbouring untypeable plasmid in four carbapenem-resistant Serratia marcescens isolates, which was characterized as an 'IncX6-like' plasmid.
Antimicrobial resistant bacteria recovered from retail ground meat products in the US include a Raoultella ornithinolytica co-harboring bla(KPC-2) and bla(NDM-5).
The study identified a Raoultella ornithinolytica isolate from retail pork sausage that co-harbors bla(KPC-2) and bla(NDM-5), which confer resistance to carbapenems. The isolate was multi-drug resistant and showed resistance to 20 different antimicrobials.
Characterization of resistance mechanisms of Enterobacter cloacae Complex co-resistant to carbapenem and colistin.
The study identified blaKPC-2, blaNDM-1, mcr-4.3, ecr, ampC, blaCTX-M-14, blaCTX-M-9, blaSHV, and blaTEM as genes contributing to carbapenem and colistin resistance in Enterobacter cloacae Complex strains.
Spread of Carbapenem-Resistant Klebsiella pneumoniae in an Intensive Care Unit: A Whole-Genome Sequence-Based Prospective Observational Study.
The study identified the presence of carbapenem-resistant Klebsiella pneumoniae (CRKP) in an ICU setting, with the detection of various resistance genes such as bla KPC-2, bla CTX-M-65, bla CTX-M-15, bla NDM-5, bla CTX-M-27, and bla DHA-1. These genes were found in isolates from both patients and the environment, indicating widespread contamination.
Global Distribution Patterns of Carbapenemase-Encoding Bacteria in a New Light: Clues on a Role for Ethnicity.
The study identifies various carbapenemase-encoding bacteria, including KPC, VIM, NDM, OXA-23, and OXA-48, and explores their distribution patterns in relation to ethnicity and residency.
Clinical and Molecular Characteristics of Carbapenem-Resistant Hypervirulent Klebsiella pneumoniae Isolates in a Tertiary Hospital in Shanghai, China.
The study identified the presence of the carbapenemase gene blaKPC-2 and extended-spectrum beta-lactamase gene blaSHV in all 16 CR-hvKP isolates, contributing to their multidrug resistance.
Co-harboring of Novel bla (KPC-2) Plasmid and Integrative and Conjugative Element Carrying Tn6203 in Multidrug-Resistant Pseudomonas aeruginosa.
The study identifies a novel blaKPC-2 plasmid (pP33-2) and an integrative and conjugative element (ICEP33) carrying Tn6203 in multidrug-resistant Pseudomonas aeruginosa strains. The plasmid pP33-2 is distinct from previously reported blaKPC-2 plasmids and is not transferable. ICEP33 contains resistance genes including aac(6')-IIa, ant(2")-Ia, blaCARB-2, and sul1, contributing to multidrug resistance.
QPX7728, An Ultra-Broad-Spectrum B-Lactamase Inhibitor for Intravenous and Oral Therapy: Overview of Biochemical and Microbiological Characteristics.
QPX7728 is a novel β-lactamase inhibitor with broad-spectrum activity against serine and metallo-β-lactamases, including Class A, C, D, and B enzymes. It effectively inhibits clinically relevant β-lactamases such as KPC-2, CTX-M-15, SHV-12, TEM-43, OXA-48, OXA-23, NDM-1, and VIM-1.
QPX7728, An Ultra-Broad-Spectrum B-Lactamase Inhibitor for Intravenous and Oral Therapy: Overview of Biochemical and Microbiological Characteristics.
QPX7728 is a novel beta-lactamase inhibitor with broad-spectrum activity against serine and metallo-beta-lactamases, including Class A, C, D, and B enzymes. It effectively inhibits clinically relevant beta-lactamases such as KPC-2, CTX-M-15, SHV-12, TEM-43, OXA-48, OXA-23, NDM-1, and VIM-1.
Activity of meropenem/vaborbactam and comparators against Gram-negative isolates from Eastern and Western European patients hospitalized with pneumonia including ventilator-associated pneumonia (2014-19).
The study characterizes various carbapenemase genes, including bla KPC-3, bla KPC-2, bla KPC-12, bla VIM-like, bla NDM-1, and bla OXA-48-like, which confer resistance to carbapenems in Enterobacterales and Pseudomonas aeruginosa isolates from European hospitals.
Activity of meropenem/vaborbactam and comparators against Gram-negative isolates from Eastern and Western European patients hospitalized with pneumonia including ventilator-associated pneumonia (2014-19).
The study characterizes various carbapenemase genes, including bla KPC-3, bla KPC-2, bla KPC-12, bla VIM-like, bla NDM-1, and bla OXA-48-like, which confer resistance to carbapenems in Enterobacterales and Pseudomonas aeruginosa isolates from European hospitals.
Activity of meropenem/vaborbactam and comparators against Gram-negative isolates from Eastern and Western European patients hospitalized with pneumonia including ventilator-associated pneumonia (2014-19).
The study characterizes various carbapenemase genes, including bla KPC-3, bla KPC-2, bla KPC-12, bla VIM-like, bla NDM-1, and bla OXA-48-like, which confer resistance to carbapenems in Enterobacterales and Pseudomonas aeruginosa isolates from European hospitals.
Plasmid analysis of NDM metallo-β-lactamase-producing Enterobacterales isolated in Vietnam.
The study characterizes bla NDM-1 and bla NDM-4 carrying plasmids in Enterobacterales isolates from Vietnam, highlighting their role in carbapenem resistance and horizontal gene transfer.
Evolutionary Trajectories toward Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Clinical Isolates.
The study identifies nine distinct CZA-resistant KPC-3 variants, highlighting the emergence of mutations in the Ω loop and 270-loop of the KPC-3 protein, leading to resistance against ceftazidime-avibactam.
Evolutionary Trajectories toward Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Clinical Isolates.
The study identifies nine distinct CZA-resistant KPC-3 variants, highlighting the emergence of mutations in the Ω loop and 270-loop of the KPC-3 protein, leading to resistance against ceftazidime-avibactam.
Evolutionary Trajectories toward Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Clinical Isolates.
The study identifies nine distinct CZA-resistant KPC-3 variants, highlighting the emergence of mutations in the Ω loop and 270-loop of the KPC-3 protein, leading to resistance against ceftazidime-avibactam.
Evolutionary Trajectories toward Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Clinical Isolates.
The study identifies nine distinct CZA-resistant KPC-3 variants, highlighting the emergence of mutations in the Ω loop and 270-loop of the KPC-3 protein, leading to resistance against ceftazidime-avibactam.
Evolutionary Trajectories toward Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Clinical Isolates.
The study identifies nine distinct CZA-resistant KPC-3 variants, highlighting the emergence of mutations in the Ω loop and 270-loop of the KPC-3 protein, leading to resistance against ceftazidime-avibactam.
Evolutionary Trajectories toward Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Clinical Isolates.
The study identifies nine distinct CZA-resistant KPC-3 variants, highlighting the emergence of mutations in the Ω loop and 270-loop of the KPC-3 protein, leading to resistance against ceftazidime-avibactam.
Evolutionary Trajectories toward Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Clinical Isolates.
The study identifies nine distinct CZA-resistant KPC-3 variants, highlighting the emergence of mutations in the Ω loop and 270-loop of the KPC-3 protein, leading to resistance against ceftazidime-avibactam.
Evolutionary Trajectories toward Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Clinical Isolates.
The study identifies nine distinct CZA-resistant KPC-3 variants, highlighting the emergence of mutations in the Ω loop and 270-loop of the KPC-3 protein, leading to resistance against ceftazidime-avibactam.
Evolutionary Trajectories toward Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Clinical Isolates.
The study identifies nine distinct CZA-resistant KPC-3 variants, highlighting the emergence of mutations in the Ω loop and 270-loop of the KPC-3 protein, leading to resistance against ceftazidime-avibactam.
Evolutionary Trajectories toward Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Clinical Isolates.
Evolutionary Trajectories toward Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Clinical Isolates.
Evolutionary Trajectories toward Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Clinical Isolates.
Evolutionary Trajectories toward Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Clinical Isolates.
Evolutionary Trajectories toward Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Clinical Isolates.
Evolutionary Trajectories toward Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Clinical Isolates.
Evolutionary Trajectories toward Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Clinical Isolates.
Evolutionary Trajectories toward Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Clinical Isolates.
Evolutionary Trajectories toward Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Clinical Isolates.
Evolutionary Trajectories toward Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Clinical Isolates.
Evolutionary Trajectories toward Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Clinical Isolates.
Evolutionary Trajectories toward Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Clinical Isolates.
Evolutionary Trajectories toward Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Clinical Isolates.
Detection of expanded-spectrum cephalosporin hydrolysis by lateral flow immunoassay.
The LFIA-CTX test effectively detects expanded-spectrum cephalosporin hydrolysis by various beta-lactamases, including CTX-M, OXA-48, KPC, NDM, and VIM, demonstrating high sensitivity and specificity.
Evidence of an epidemic spread of KPC-producing Enterobacterales in Czech hospitals.
The study identifies blaKPC-2 and blaKPC-3 as the primary genes responsible for carbapenem resistance in KPC-producing Enterobacterales, highlighting the role of various plasmid types in the spread of these resistance genes.
Genomic characterization of carbapenem-non-susceptible Pseudomonas aeruginosa in Singapore.
The study identified multiple carbapenemase genes (blaIMP, blaNDM-1, blaVIM, blaGES-5, blaKPC-2) and chromosomal mutations (ampD, ampR, dacB, mexZ, armZ, nalD, oprD, gyrA, parC) associated with carbapenem resistance in Pseudomonas aeruginosa isolates from Singapore.
Increased gene expression and copy number of mutated bla(KPC) lead to high-level ceftazidime/avibactam resistance in Klebsiella pneumoniae.
Mutated blaKPC genes, particularly blaKPC-51, contribute to high-level ceftazidime/avibactam resistance in Klebsiella pneumoniae through increased gene expression and copy number.
Carbapenem-Resistant Klebsiella pneumoniae in Southwest China: Molecular Characteristics and Risk Factors Caused by KPC and NDM Producers.
The study identified bla KPC-2, bla NDM-1, bla NDM-5, and bla IMP-4 as the main carbapenemase genes in CRKP isolates, highlighting their role in carbapenem resistance and the emergence of novel clones like ST4495.
Genomic insights into the diversity, virulence and resistance of Klebsiella pneumoniae extensively drug resistant clinical isolates.
The study identified various beta-lactamase genes, including blaSHV-11, blaKPC-2, and blaNDM-1, along with qnrS1, aadA1, dfrA1, and sul1, which contribute to multidrug resistance in extensively drug-resistant Klebsiella pneumoniae isolates.
Direct Testing for KPC-Mediated Carbapenem Resistance from Blood Samples Using a T2 Magnetic Resonance Based Assay.
The T2Resistance™ panel successfully detected KPC-mediated carbapenem resistance in blood samples from patients with bloodstream infections caused by KPC-producing Klebsiella pneumoniae and VIM/CMY-producing Citrobacter freundii.
Bloodstream Infections caused by Klebsiella pneumoniae and Serratia marcescens isolates co-harboring NDM-1 and KPC-2.
The study identifies the presence of blaKPC-2 and blaNDM-1 genes in carbapenem-resistant Klebsiella pneumoniae and Serratia marcescens isolates causing bloodstream infections, highlighting their role in carbapenem resistance.
Multiplex lateral flow immunochromatographic assay is an effective method to detect carbapenemases without risk of OXA-48-like cross reactivity.
The study evaluates the NG-Test® CARBA 5 assay for detecting carbapenemases in Enterobacterales, showing high sensitivity and specificity. It identifies bla KPC, bla OXA-48-like, bla NDM, bla VIM, bla IMP, bla IMI, and bla SME as the main carbapenemase genes detected.
Emergence of a KPC Variant Conferring Resistance to Ceftazidime-Avibactam in a Widespread ST11 Carbapenem-Resistant Klebsiella pneumoniae Clone in China.
The study identifies a novel KPC variant, KPC-74, which confers resistance to ceftazidime-avibactam (CZA) through a deletion in the omega-loop region, leading to increased ceftazidime hydrolysis and reduced avibactam affinity.
Emergence of a KPC Variant Conferring Resistance to Ceftazidime-Avibactam in a Widespread ST11 Carbapenem-Resistant Klebsiella pneumoniae Clone in China.
Emergence of a KPC Variant Conferring Resistance to Ceftazidime-Avibactam in a Widespread ST11 Carbapenem-Resistant Klebsiella pneumoniae Clone in China.
Climatological and Epidemiological Conditions Are Important Factors Related to the Abundance of bla(KPC) and Other Antibiotic Resistance Genes (ARGs) in Wastewater Treatment Plants and Their Effluents, in an Endemic Country.
The study identified the prevalence of antibiotic resistance genes (ARGs) in wastewater treatment plants (WWTPs) in Colombia, highlighting the significant presence of bla KPC, sul 1, sul 2, erm B, and others. It also found that environmental factors such as dissolved oxygen and precipitation correlate with the abundance of these genes.
Virulence and Antibiotic Resistance Characteristics of Vibrio Isolates From Rustic Environmental Freshwaters.
The study identified several antibiotic resistance genes in Vibrio isolates from environmental freshwater sources, including aadA, strA, aphA1, catII, ampC, blaTEM, blaGES, blaOXA-48, blaIMP, blaVIM, blaKPC, and qnrVC, indicating a significant risk of antimicrobial resistance in these bacteria.
Characterization of Emerging Pathogens Carrying bla(KPC-2) Gene in IncP-6 Plasmids Isolated From Urban Sewage in Argentina.
The study identifies blaKPC-2 gene in IncP-6 plasmids in K. quasipneumoniae and E. asburiae isolated from urban sewage in Argentina, highlighting the role of these plasmids in the spread of carbapenem resistance.
Emergence of Klebsiella pneumoniae ST307 Co-Producing CTX-M with SHV and KPC from Paediatric Patients at Shenzhen Children's Hospital, China.
The study identifies the emergence of Klebsiella pneumoniae ST307 co-producing CTX-M with SHV and KPC in paediatric patients at Shenzhen Children's Hospital, China. It characterizes various carbapenemase and extended-spectrum beta-lactamase encoding genes, highlighting the multidrug-resistant nature of these isolates.
Genetic Diversity of Multidrug-Resistant Pseudomonas aeruginosa Isolates Carrying bla (VIM-2) and bla (KPC-2) Genes That Spread on Different Genetic Environment in Colombia.
The study identifies bla(VIM-2) and bla(KPC-2) genes in multidrug-resistant Pseudomonas aeruginosa isolates from Colombia, highlighting their diverse genetic environments and mechanisms of resistance.
Genomic Features Associated with the Degree of Phenotypic Resistance to Carbapenems in Carbapenem-Resistant Klebsiella pneumoniae.
The study identifies bla KPC-3, bla KPC-2, bla OXA-232, and mutations in ompK35, ompK36, and ompK37 as significant contributors to carbapenem resistance in Klebsiella pneumoniae isolates.
Genomic Features Associated with the Degree of Phenotypic Resistance to Carbapenems in Carbapenem-Resistant Klebsiella pneumoniae.
The study identifies bla KPC-3, bla KPC-2, bla OXA-232, and mutations in ompK35, ompK36, and ompK37 as significant contributors to carbapenem resistance in Klebsiella pneumoniae isolates.
Genomic Features Associated with the Degree of Phenotypic Resistance to Carbapenems in Carbapenem-Resistant Klebsiella pneumoniae.
The study identifies bla KPC-3, bla KPC-2, bla OXA-232, and mutations in ompK35, ompK36, and ompK37 as significant contributors to carbapenem resistance in Klebsiella pneumoniae isolates.
Antibiotic resistance alters through iron-regulating Sigma factors during the interaction of Staphylococcus aureus and Pseudomonas aeruginosa.
The study shows that the interaction between S. aureus and P. aeruginosa influences antibiotic resistance through changes in the expression of genes such as kpc, mexA-mexB-oprM, oprD, norA, and walk/R, which are involved in carbapenem, ciprofloxacin, and vancomycin resistance.
Genetic characterization of Carbapenem-Resistant Escherichia coli from China, 2015-2017.
The study identified various carbapenemase genes, including NDM-1, NDM-4, NDM-5, NDM-6, NDM-9, KPC-2, IMP-4, and OXA-48, in carbapenem-resistant Escherichia coli isolates from China. ST167 was the most common sequence type, followed by ST410 and ST131.
The Epidemiology, Virulence and Antimicrobial Resistance of Invasive Klebsiella pneumoniae at a Children's Medical Center in Eastern China.
The study identified multiple beta-lactam resistance genes, including bla SHV-11, bla FOX-1, bla ACT-1, bla CTX-M-14, bla TEM-1, bla VIM-1, bla NDM-1, bla KPC-2, and bla OXA-1, in invasive Klebsiella pneumoniae isolates. Additionally, hypervirulence genes such as iroB, p rmpA, mrkD, wabG, Uge, fimH, and ycfM were prevalent.
Amikacin in combination with zinc pyrithione prevents growth of a multidrug-resistant carbapenem-resistant Klebsiella pneumoniae isolate.
The study identifies aminoglycoside resistance mediated by aac(6')-Ib and beta-lactam resistance conferred by blaTEM-1, blaSHV-11, blaSHV-12, and blaKPC-2 in a multidrug-resistant CRKP isolate. The combination of amikacin and zinc pyrithione effectively overcomes amikacin resistance.
Mutations in porin LamB contribute to ceftazidime-avibactam resistance in KPC-producing Klebsiella pneumoniae.
Selection and characterization of mutational resistance to aztreonam/avibactam in β-lactamase-producing Enterobacterales.
Detection and Characterization of Targeted Carbapenem-Resistant Health Care-Associated Threats: Findings from the Antibiotic Resistance Laboratory Network, 2017 to 2019.
The study identified the prevalence of targeted carbapenemase genes (bla KPC, bla NDM, bla OXA-48-like, bla VIM, and bla IMP) in carbapenem-resistant Enterobacterales, Pseudomonas aeruginosa, and Acinetobacter baumannii. bla KPC was the most common gene detected in CRE isolates, while bla VIM was prevalent in CP-CRPA isolates. Regional variations in the frequency of these genes were observed.
OXA-48 Carbapenemase-Encoding Transferable Plasmids of Klebsiella pneumoniae Recovered from Egyptian Patients Suffering from Complicated Urinary Tract Infections.
The study identified the presence of carbapenemase genes, including bla OXA-48, bla VIM, bla KPC, and bla NDM, in carbapenem-resistant Gram-negative bacteria from Egyptian patients with complicated urinary tract infections. These genes were successfully transferred to E. coli, demonstrating their potential for horizontal gene transfer.
Environmental and Pathogenic Carbapenem Resistant Bacteria Isolated from a Wastewater Treatment Plant Harbour Distinct Antibiotic Resistance Mechanisms.
The study identified various carbapenem resistance genes, including blaKPC, blaOXA-48, and blaVIM, in pathogenic bacteria isolated from wastewater treatment plants, highlighting the diverse resistance mechanisms in these isolates.
Characterization of ESBL-Producing Escherichia coli and Klebsiella pneumoniae Isolated from Clinical Samples in a Northern Portuguese Hospital: Predominance of CTX-M-15 and High Genetic Diversity.
The study identified the predominance of CTX-M-15 and high genetic diversity among ESBL-producing E. coli and K. pneumoniae isolates, including bla CTX-M-15, bla CTX-M-27, bla CTX-M-1, bla KPC-2/3, bla SHV-12, bla SHV-27, bla SHV-11, bla SHV-28, tet(A), and tet(B) genes.
Whole-Genome Sequencing (WGS) of Carbapenem-Resistant K. pneumoniae Isolated in Long-Term Care Facilities in the Northern Italian Region.
The study identified various carbapenem-resistance genes including blaKPC-2, blaKPC-3, blaKPC-9, blaSHV-11, blaSHV-28, blaCTX-M-15, blaOXA-1, blaOXA-9, blaOXA-23, qnrS1, qnrB19, qnrB66, aac(6')-Ib-cr, and fosA in carbapenem-resistant K. pneumoniae isolates from long-term care facilities in northern Italy.
Whole-Genome Sequencing (WGS) of Carbapenem-Resistant K. pneumoniae Isolated in Long-Term Care Facilities in the Northern Italian Region.
The study identified various carbapenem-resistance genes including blaKPC-2, blaKPC-3, blaKPC-9, blaSHV-11, blaSHV-28, blaCTX-M-15, blaOXA-1, blaOXA-9, blaOXA-23, qnrS1, qnrB19, qnrB66, aac(6')-Ib-cr, and fosA in carbapenem-resistant K. pneumoniae isolates from long-term care facilities in northern Italy.
Whole-Genome Sequencing (WGS) of Carbapenem-Resistant K. pneumoniae Isolated in Long-Term Care Facilities in the Northern Italian Region.
The study identified various carbapenem-resistance genes including blaKPC-2, blaKPC-3, blaKPC-9, blaSHV-11, blaSHV-28, blaCTX-M-15, blaOXA-1, blaOXA-9, blaOXA-23, qnrS1, qnrB19, qnrB66, aac(6')-Ib-cr, and fosA in carbapenem-resistant K. pneumoniae isolates from long-term care facilities in northern Italy.
Application of a multiplex immunochromatographic assay for rapid identification of carbapenemases in a clinical microbiology laboratory: performance and turn-around-time evaluation of NG-test Carba 5.
The Carba 5 assay effectively identifies five major carbapenemases (KPC, NDM, VIM, IMP, and OXA-48-like) with high sensitivity and specificity, offering a faster turnaround time compared to Xpert Carba-R.
Performance Evaluation of the Gradient Diffusion Strip Method and Disk Diffusion Method for Ceftazidime-Avibactam Against Enterobacterales and Pseudomonas aeruginosa: A Dual-Center Study.
The study evaluated the performance of the gradient diffusion strip method and disk diffusion method for determining susceptibility to ceftazidime-avibactam against Enterobacterales and Pseudomonas aeruginosa. It identified various carbapenemase genes, including bla KPC, bla NDM, bla IMP, and bla VIM, which confer resistance to ceftazidime-avibactam.
Clinical and Bacterial Characteristics of Klebsiella pneumoniae Affecting 30-Day Mortality in Patients With Bloodstream Infection.
The study identified bla KPC-2 as a carbapenem resistance gene and iutA and Kvar_1549 as virulence factors associated with increased 30-day mortality in patients with Klebsiella pneumoniae bloodstream infection.
KPC-39-Mediated Resistance to Ceftazidime-Avibactam in a Klebsiella pneumoniae ST307 Clinical Isolate.
The study identifies KPC-39, a novel KPC-3 variant with a single amino acid substitution (A172T), which mediates resistance to ceftazidime-avibactam in a Klebsiella pneumoniae ST307 clinical isolate.
KPC-39-Mediated Resistance to Ceftazidime-Avibactam in a Klebsiella pneumoniae ST307 Clinical Isolate.
KPC-39-Mediated Resistance to Ceftazidime-Avibactam in a Klebsiella pneumoniae ST307 Clinical Isolate.
KPC-39-Mediated Resistance to Ceftazidime-Avibactam in a Klebsiella pneumoniae ST307 Clinical Isolate.
Expansion of KPC-producing Enterobacterales in four large hospitals in Hanoi, Vietnam.
The study identifies bla KPC-2, bla KPC-12, and bla KPC-14 as the primary carbapenem resistance genes in KPC-producing Enterobacterales in Hanoi, Vietnam, highlighting their prevalence and diversity.
Expansion of KPC-producing Enterobacterales in four large hospitals in Hanoi, Vietnam.
The study identifies bla KPC-2, bla KPC-12, and bla KPC-14 as the primary carbapenem resistance genes in KPC-producing Enterobacterales in Hanoi, Vietnam, highlighting their prevalence and diversity.
Expansion of KPC-producing Enterobacterales in four large hospitals in Hanoi, Vietnam.
The study identifies bla KPC-2, bla KPC-12, and bla KPC-14 as the primary carbapenem resistance genes in KPC-producing Enterobacterales in Hanoi, Vietnam, highlighting their prevalence and diversity.
Analysis of Carbapenemase-Resistant Genotypes of Highly Virulent Klebsiella pneumoniae and Clinical Infection Characteristics of Different MLST Types.
The study identified KPC, IMP, VIM, and NDM carbapenemase genes in 74 CR-KP strains, with KPC being the most prevalent. ST11 was the dominant MLST type and was associated with higher resistance to carbapenems and poorer patient outcomes.
Using Cy5-dUTP labelling of RPA-amplicons with downstream microarray analysis for the detection of antibiotic resistance genes.
The study describes a novel method for detecting antibiotic resistance genes using Cy5-dUTP labeling of RPA-amplicons followed by microarray analysis. It successfully identified bla CTX-M15, bla KPC, bla NDM, and bla VIM genes in E. coli and P. aeruginosa.
In vitro activity of imipenem/relebactam against Gram-negative clinical isolates in two Spanish tertiary hospitals.
Imipenem/relebactam showed high activity against KPC carbapenemase-producing Enterobacterales and against carbapenem-resistant P. aeruginosa without VIM enzymes, but had limited effectiveness against OXA-48 and A. baumannii.
Molecular Epidemiological Analysis of ST11-K64 Extensively Drug-Resistant Klebsiella pneumoniae Infections Outbreak in Intensive Care and Neurosurgery Units Based on Whole-Genome Sequencing.
The study identified multiple AMR genes in ST11-K64 XDRKp strains, including beta-lactamases, aminoglycoside resistance genes, and efflux pumps, contributing to extensive drug resistance.
Transmission Dynamics of Carbapenem-Resistant Klebsiella pneumoniae Sequence Type 11 Strains Carrying Capsular Loci KL64 and rmpA/rmpA2 Genes.
The study identified multiple AMR genes and mutations in ST11-KL64 CRKP isolates, including bla KPC-2, rmtB, catA2, fosA, qnrS1, sul2, tet(A), aadA2, dfrA14, bla CTX-M-65, bla SHV-12, bla TEM-1B, iucABCD, iutA, rmpA2, and mutations in GyrA (S83I, D87G) and ParC (S80I).
Prevalence and characterisation of carbapenemase encoding genes in multidrug-resistant Gram-negative bacilli.
The study identified various carbapenemase genes, including bla KPC, bla NDM, bla VIM, bla IMP, and bla OXA-48, in multidrug-resistant Gram-negative bacilli, highlighting their prevalence and contribution to carbapenem resistance.
Within patient genetic diversity of bla(KPC) harboring Klebsiella pneumoniae in a Colombian hospital and identification of a new NTE(KPC) platform.
The study identifies a new NTE(KPC) platform, Tn 6454, responsible for blaKPC-2 mobilization in non-CC258 KPC-Kp isolates. It also characterizes the genetic diversity of blaKPC-harboring Klebsiella pneumoniae isolates and highlights the role of different genetic platforms in the dissemination of carbapenem resistance.
Within patient genetic diversity of bla(KPC) harboring Klebsiella pneumoniae in a Colombian hospital and identification of a new NTE(KPC) platform.
The study identifies a new NTE(KPC) platform, Tn 6454, responsible for blaKPC-2 mobilization in non-CC258 KPC-Kp isolates. It also characterizes the genetic diversity of blaKPC-harboring Klebsiella pneumoniae isolates and highlights the role of different genetic platforms in the dissemination of carbapenem resistance.
Within patient genetic diversity of bla(KPC) harboring Klebsiella pneumoniae in a Colombian hospital and identification of a new NTE(KPC) platform.
The study identifies a new NTE(KPC) platform, Tn 6454, responsible for blaKPC-2 mobilization in non-CC258 KPC-Kp isolates. It also characterizes the genetic diversity of blaKPC-harboring Klebsiella pneumoniae isolates and highlights the role of different genetic platforms in the dissemination of carbapenem resistance.
Emergence of Ceftazidime- and Avibactam-Resistant Klebsiella pneumoniae Carbapenemase-Producing Pseudomonas aeruginosa in China.
The study identifies blaKPC-2 as a key determinant of ceftazidime-avibactam resistance in KPC-producing Pseudomonas aeruginosa, highlighting the role of plasmid-mediated gene copy number variations in resistance development.
Molecular Analysis of bla(KPC-2)-Harboring Plasmids: Tn4401a Interplasmid Transposition and Tn4401a-Carrying ColRNAI Plasmid Mobilization from Klebsiella pneumoniae to Citrobacter europaeus and Morganella morganii in a Single Patient.
The study identifies blaKPC-2 as a gene responsible for carbapenem resistance in Klebsiella pneumoniae, Citrobacter europaeus, and Morganella morganii strains isolated from a single patient, highlighting the role of Tn4401a-containing plasmids in the dissemination of this resistance gene.
Co-occurrence of Klebsiella variicola and Klebsiella pneumoniae Both Carrying bla (KPC) from a Respiratory Intensive Care Unit Patient.
The study identified Klebsiella variicola and Klebsiella pneumoniae strains carrying multiple resistance genes, including bla KPC-2, bla TEM-1A, bla LEN17, aadA16, arr-3, qnrB4, oqxA/B, dfrA27, sul1, tetD, fosA, qacEΔ1, bla CTX-M-3, bla TEM-1B, bla CTX-M-65, bla SHV-27, aac(6')-IIa, rmtB, aph(3')-Ia, aadA16, qnrS1, aac(6')-Ib-cr, qnrB91, oqxA/B, mph(A), tet(A), fosA, dfrA27, and two copies of qacEΔ1-sul1.
Diagnosis and Treatment of Bacterial Pneumonia in Critically Ill Patients with COVID-19 Using a Multiplex PCR Assay: A Large Italian Hospital's Five-Month Experience.
The study evaluated the FilmArray pneumonia plus (FA-PP) panel for diagnosing bacterial pneumonia in critically ill COVID-19 patients, highlighting its utility in guiding antimicrobial therapy. Key AMR genes identified include mecA/-C, CTX-M, KPC, and bla oxa-23.
PangenomeNet: a pan-genome-based network reveals functional modules on antimicrobial resistome for Escherichia coli strains.
The study identifies putative resistance genes, including blaKPC, transglycosylases, and hypothetical proteins, through a pan-genome-based network analysis, highlighting their roles in meropenem and beta-lactam resistance.
Emergence of Ceftazidime/Avibactam and Tigecycline Resistance in Carbapenem-Resistant Klebsiella pneumoniae Due to In-Host Microevolution.
The study identifies blaKPC-2 duplication, ramR inactivation, and wbbL disruption as key mechanisms for CZA and TGC resistance in CRKP strains.
Bloodstream Infections Caused by Klebsiella pneumoniae Carbapenemase-Producing P. aeruginosa Sequence Type 463, Associated With High Mortality Rates in China: A Retrospective Cohort Study.
The study identifies bla KPC-2 and bla KPC-33 as the primary carbapenem resistance genes in ST463 CRPA isolates, along with exoU and exoS virulence genes. These findings highlight the association of ST463 CRPA with high mortality rates and multidrug resistance.
Bloodstream Infections Caused by Klebsiella pneumoniae Carbapenemase-Producing P. aeruginosa Sequence Type 463, Associated With High Mortality Rates in China: A Retrospective Cohort Study.
The study identifies bla KPC-2 and bla KPC-33 as the primary carbapenem resistance genes in ST463 CRPA isolates, along with exoU and exoS virulence genes. These findings highlight the association of ST463 CRPA with high mortality rates and multidrug resistance.
Bloodstream Infections Caused by Klebsiella pneumoniae Carbapenemase-Producing P. aeruginosa Sequence Type 463, Associated With High Mortality Rates in China: A Retrospective Cohort Study.
The study identifies bla KPC-2 and bla KPC-33 as the primary carbapenem resistance genes in ST463 CRPA isolates, along with exoU and exoS virulence genes. These findings highlight the association of ST463 CRPA with high mortality rates and multidrug resistance.
Clonal spread of carbapenem-resistant Klebsiella pneumoniae among patients at admission and discharge at a Vietnamese neonatal intensive care unit.
The study identified several carbapenem resistance genes, including blaKPC-2, blaNDM-1, blaNDM-4, blaNDM-5, and blaOXA-181, as well as 16S rRNA methylase genes rmtB, rmtC, and armA, and the colistin resistance gene mcr-8 in carbapenem-resistant Klebsiella pneumoniae isolates from a Vietnamese NICU.
Geographic Patterns of Carbapenem-Resistant Pseudomonas aeruginosa in the Asia-Pacific Region: Results from the Antimicrobial Testing Leadership and Surveillance (ATLAS) Program, 2015-2019.
The study identified various carbapenemase genes, including blaVIM, blaNDM, blaVEB, blaIMP, blaGES, blaTEM, and blaKPC, in carbapenem-resistant Pseudomonas aeruginosa isolates from the Asia-Pacific region.
Co-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae Strain.
The study characterizes a carbapenem-resistant hypervirulent Klebsiella pneumoniae strain that harbors a conjugative virulence plasmid and a bla KPC–2-bearing plasmid. The virulence plasmid contains multiple resistance genes, including bla KPC–2, qnrB4, bla DHA–1, sul1, msr(E), mph(E), bla TEM–1B, aac(3)-IId, bla SHV–12, mph(A), bla CTX–M–65, bla TEM–1B, fosA3, and rmtB. The study also shows that these plasmids can be transferred to other bacterial strains, contributing to the spread of multidrug-resistant and hypervirulent K. pneumoniae.
Direct detection of intact Klebsiella pneumoniae carbapenemase variants from cell lysates: Identification, characterization and clinical implications.
The study identifies and characterizes intact KPC-2, KPC-3, KPC-4, and KPC-5 variants using mass spectrometry, demonstrating their resistance to carbapenems, penicillins, and cephalosporins.
Direct detection of intact Klebsiella pneumoniae carbapenemase variants from cell lysates: Identification, characterization and clinical implications.
The study identifies and characterizes intact KPC-2, KPC-3, KPC-4, and KPC-5 variants using mass spectrometry, demonstrating their resistance to carbapenems, penicillins, and cephalosporins.
Direct detection of intact Klebsiella pneumoniae carbapenemase variants from cell lysates: Identification, characterization and clinical implications.
The study identifies and characterizes intact KPC-2, KPC-3, KPC-4, and KPC-5 variants using mass spectrometry, demonstrating their resistance to carbapenems, penicillins, and cephalosporins.
Ceftazidime/Avibactam-Resistant Klebsiella pneumoniae subsp. pneumoniae Isolates in a Tertiary Italian Hospital: Identification of a New Mutation of the Carbapenemase Type 3 (KPC-3) Gene Conferring Ceftazidime/Avibactam Resistance.
The study identifies a new mutation in the KPC-3 gene (Q168L) that confers resistance to ceftazidime/avibactam in Klebsiella pneumoniae subsp. pneumoniae isolates.
In vitro activity of the orally bioavailable ceftibuten/VNRX-7145 (VNRX-5236 etzadroxil) combination against a challenge set of Enterobacterales pathogens carrying molecularly characterized beta-lactamase genes.
The study evaluated the in vitro activity of ceftibuten combined with VNRX-5236 against Enterobacterales pathogens carrying various beta-lactamase genes, including bla OXA-48, bla OXA-232, bla KPC-3, and bla KPC-2. VNRX-5236 significantly reduced the MIC of ceftibuten against these isolates, demonstrating effective restoration of ceftibuten activity against beta-lactamase-producing Enterobacterales.
In vitro activity of the orally bioavailable ceftibuten/VNRX-7145 (VNRX-5236 etzadroxil) combination against a challenge set of Enterobacterales pathogens carrying molecularly characterized beta-lactamase genes.
The study evaluated the in vitro activity of ceftibuten combined with VNRX-5236 against Enterobacterales pathogens carrying various beta-lactamase genes, including bla OXA-48, bla OXA-232, bla KPC-3, and bla KPC-2. VNRX-5236 significantly reduced the MIC of ceftibuten against these isolates, demonstrating effective restoration of ceftibuten activity against beta-lactamase-producing Enterobacterales.
Current and future perspectives in the treatment of multidrug-resistant Gram-negative infections.
The paper discusses the mechanisms of resistance in multidrug-resistant Gram-negative bacteria, focusing on beta-lactamases such as KPC, NDM, VIM, IMP, and OXA-48, as well as efflux pumps like adeABC, ampC, mexAB-OprM, mexXY-OprM, mexCD-OprJ, and acrAB-TolC.
Deciphering the Epidemiological Characteristics and Molecular Features of bla (KPC-2)- or bla (NDM-1)-Positive Klebsiella pneumoniae Isolates in a Newly Established Hospital.
The study identified bla KPC-2 and bla NDM-1 as the primary carbapenem resistance genes in Klebsiella pneumoniae isolates from a newly established hospital. bla KPC-2 was more prevalent and associated with broader resistance profiles compared to bla NDM-1.
Deciphering the Epidemiological Characteristics and Molecular Features of bla (KPC-2)- or bla (NDM-1)-Positive Klebsiella pneumoniae Isolates in a Newly Established Hospital.
The study identified bla KPC-2 and bla NDM-1 as the primary carbapenem resistance genes in Klebsiella pneumoniae isolates from a newly established hospital. bla KPC-2 was more prevalent and associated with broader resistance profiles compared to bla NDM-1.
Carbapenem-Resistant Citrobacter spp. as an Emerging Concern in the Hospital-Setting: Results From a Genome-Based Regional Surveillance Study.
The study identifies various carbapenemase genes, including bla KPC-2, bla OXA-48, bla VIM-1, bla NDM-5, bla OXA-162, and bla KPC-3, in Citrobacter spp. isolates, highlighting their role in carbapenem resistance.
Carbapenem-Resistant Citrobacter spp. as an Emerging Concern in the Hospital-Setting: Results From a Genome-Based Regional Surveillance Study.
The study identifies various carbapenemase genes, including bla KPC-2, bla OXA-48, bla VIM-1, bla NDM-5, bla OXA-162, and bla KPC-3, in Citrobacter spp. isolates, highlighting their role in carbapenem resistance.
Engineered CRISPR-Cas systems for the detection and control of antibiotic-resistant infections.
This review discusses the application of CRISPR-Cas systems in the detection and control of antibiotic-resistant infections, highlighting their potential as tools for targeted elimination of antibiotic resistance genes and restoration of bacterial susceptibility to antibiotics.
Clonal Spread and Intra- and Inter-Species Plasmid Dissemination Associated With Klebsiella pneumoniae Carbapenemase-Producing Enterobacterales During a Hospital Outbreak in Barcelona, Spain.
The study reports a hospital outbreak caused by KPC-producing Enterobacterales, primarily Klebsiella pneumoniae, with the blaKPC-2 gene being the main resistance determinant. The gene was found in various plasmid types, including IncFIIk, and was associated with carbapenem resistance.
Clonal Spread and Intra- and Inter-Species Plasmid Dissemination Associated With Klebsiella pneumoniae Carbapenemase-Producing Enterobacterales During a Hospital Outbreak in Barcelona, Spain.
The study reports a hospital outbreak caused by KPC-producing Enterobacterales, primarily Klebsiella pneumoniae, with the blaKPC-2 gene being the main resistance determinant. The gene was found in various plasmid types, including IncFIIk, and was associated with carbapenem resistance.
Antibacterial effect of cerium oxide nanoparticle against Pseudomonas aeruginosa.
The study found that cerium oxide nanoparticles (CNPs) and Scaffold@CNPs significantly reduced the expression of the genes shv, kpc, and imp in Pseudomonas aeruginosa, indicating their potential to combat antibiotic resistance.
Analyses of the response of carbapenem-resistant Pseudomonas aeruginosa against monotherapy and combined therapy using quantum dots and proteomics.
The study identified the blaKPC gene as a significant contributor to carbapenem resistance in Pseudomonas aeruginosa isolates, highlighting its role in biofilm formation and resistance mechanisms.
A pentaplex real-time PCR assay for rapid identification of major beta-lactamase genes KPC, NDM, CTX, CMY, and OXA-48 directly from bacteria in blood.
The study presents a pentaplex real-time PCR assay for the rapid identification of major beta-lactamase genes KPC, NDM, CTX, CMY, and OXA-48 directly from bacteria in blood.
High Prevalence of Klebsiella pneumoniae Infections in AnHui Province: Clinical Characteristic and Antimicrobial Resistance.
The study identified carbapenemase genes KPC-2, NDM-1, VIM-1, IMP-1, and OXA-48 in hypervirulent Klebsiella pneumoniae isolates, highlighting the high prevalence of carbapenem-resistant strains in Anhui province.
A Novel Multidrug Resistant, Non-Tn4401 Genetic Element-Bearing, Strain of Klebsiella pneumoniae Isolated From an Urban Lake With Drinking and Recreational Water Reuse.
The study identifies a multidrug-resistant Klebsiella pneumoniae strain, ST5236, carrying the beta-lactamase gene blaCTX-M-15, the carbapenemase gene blaKPC-2, and the fosfomycin resistance gene fosA, along with efflux pumps oqxAB and acrAB, and heavy metal resistance genes fieF and arsC.
Clonal groups of extended-spectrum beta-lactamase and biofilm producing uropathogenic Escherichia coli in Iran.
The study identified various beta-lactamase genes, including bla TEM, bla CTX-M, bla OXA-1, bla IMP, bla VIM, bla KPC, and bla OXA-48, in biofilm-producing uropathogenic Escherichia coli strains from Iran, highlighting their role in multidrug resistance.
Comparison of novel rapid diagnostic of blood culture identification and antimicrobial susceptibility testing by Accelerate Pheno system and BioFire FilmArray Blood Culture Identification and BioFire FilmArray Blood Culture Identification 2 panels.
The study evaluated the performance of the Accelerate Pheno system, BioFire FilmArray BCID, and BCID2 panels for rapid blood culture identification and antimicrobial susceptibility testing. It identified several AMR genes including bla KPC, bla NDM, bla OXA-48, bla VIM, bla IMP, mecA, vanA, vanB, mcr-1, and CTX-M, which confer resistance to carbapenems, beta-lactams, glycopeptides, polymyxins, and cephalosporins.
Rapid Detection of bla(KPC), bla(NDM), bla(OXA-48-like) and bla(IMP) Carbapenemases in Enterobacterales Using Recombinase Polymerase Amplification Combined With Lateral Flow Strip.
The study developed a rapid RPA-LFS assay for detecting four major carbapenemase genes (blaKPC, blaNDM, blaOXA-48-like, and blaIMP) in Enterobacterales, demonstrating high specificity and sensitivity.
Comparison of Carbapenem-Resistant Klebsiella pneumoniae Strains Causing Intestinal Colonization and Extraintestinal Infections: Clinical, Virulence, and Molecular Epidemiological Characteristics.
The study identified blaKPC-2 as the predominant carbapenemase gene in carbapenem-resistant Klebsiella pneumoniae (CRKP) strains, with high prevalence in both intestinal colonization and extraintestinal infection groups.
Comparison of Four Carbapenemase Detection Methods for bla(KPC-2) Variants.
The study compared four carbapenemase detection methods for bla(KPC-2) variants and found that GeneXpert Carba-R was the most reliable method for detecting all bla KPC-2 variants, whereas mCIM and APB/EDTA failed to detect certain variants.
Comparison of Four Carbapenemase Detection Methods for bla(KPC-2) Variants.
The study compared four carbapenemase detection methods for bla(KPC-2) variants and found that GeneXpert Carba-R was the most reliable method for detecting all bla KPC-2 variants, whereas mCIM and APB/EDTA failed to detect certain variants.
Comparison of Four Carbapenemase Detection Methods for bla(KPC-2) Variants.
The study compared four carbapenemase detection methods for bla(KPC-2) variants and found that GeneXpert Carba-R was the most reliable method for detecting all bla KPC-2 variants, whereas mCIM and APB/EDTA failed to detect certain variants.
Comparison of Four Carbapenemase Detection Methods for bla(KPC-2) Variants.
The study compared four carbapenemase detection methods for bla(KPC-2) variants and found that GeneXpert Carba-R was the most reliable method for detecting all bla KPC-2 variants, whereas mCIM and APB/EDTA failed to detect certain variants.
Comparison of Four Carbapenemase Detection Methods for bla(KPC-2) Variants.
The study compared four carbapenemase detection methods for bla(KPC-2) variants and found that GeneXpert Carba-R was the most reliable method for detecting all bla KPC-2 variants, whereas mCIM and APB/EDTA failed to detect certain variants.
Comparison of Four Carbapenemase Detection Methods for bla(KPC-2) Variants.
The study compared four carbapenemase detection methods for bla(KPC-2) variants and found that GeneXpert Carba-R was the most reliable method for detecting all bla KPC-2 variants, whereas mCIM and APB/EDTA failed to detect certain variants.
Comparison of Four Carbapenemase Detection Methods for bla(KPC-2) Variants.
Comparison of Four Carbapenemase Detection Methods for bla(KPC-2) Variants.
Comparison of Four Carbapenemase Detection Methods for bla(KPC-2) Variants.
Comparison of Four Carbapenemase Detection Methods for bla(KPC-2) Variants.
Comparison of Four Carbapenemase Detection Methods for bla(KPC-2) Variants.
Comparison of Four Carbapenemase Detection Methods for bla(KPC-2) Variants.
Molecular Mechanisms Driving the In Vivo Development of KPC-71-Mediated Resistance to Ceftazidime-Avibactam during Treatment of Carbapenem-Resistant Klebsiella pneumoniae Infections.
The study identifies a novel KPC variant, KPC-71, which mediates resistance to ceftazidime-avibactam (CZA) through an insertion of a serine between Ambler positions 182 and 183. This mutation increases ceftazidime affinity and reduces avibactam inhibition, leading to CZA resistance.
Molecular Mechanisms Driving the In Vivo Development of KPC-71-Mediated Resistance to Ceftazidime-Avibactam during Treatment of Carbapenem-Resistant Klebsiella pneumoniae Infections.
Molecular Mechanisms Driving the In Vivo Development of KPC-71-Mediated Resistance to Ceftazidime-Avibactam during Treatment of Carbapenem-Resistant Klebsiella pneumoniae Infections.
Antibiotic resistance and phylogenetic profiling of Escherichia coli from dairy farm soils; organic versus conventional systems.
The study identified the presence of beta-lactamase genes in E. coli isolates from dairy farm soils, indicating resistance to cefepoxy and cefoxitin. These findings highlight the potential for antimicrobial resistance dissemination in agricultural environments.
Environmental spreading of clinically relevant carbapenem-resistant gram-negative bacilli: the occurrence of bla(KPC-or-NDM) strains relates to local hospital activities.
The study identifies the presence of bla KPC and bla NDM genes in various carbapenem-resistant gram-negative bacilli, highlighting their association with hospital activities and environmental spread.
Carriage of distinct bla(KPC-2) and bla(OXA-48) plasmids in a single ST11 hypervirulent Klebsiella pneumoniae isolate in Egypt.
The study reports the co-carriage of distinct bla(KPC-2) and bla(OXA-48) plasmids in a single ST11 hypervirulent Klebsiella pneumoniae isolate in Egypt, highlighting the complex resistance mechanisms and potential for plasmid fusion.
Characterization of Carbapenem-Resistant Enterobacteriaceae Cultured From Retail Meat Products, Patients, and Porcine Excrement in China.
The study identified bla NDM-5, bla KPC-2, and mcr-1 as key resistance genes in carbapenem-resistant Enterobacteriaceae isolated from retail meat products, patients, and porcine excrement in China.
Characterization of Carbapenem-Resistant Enterobacteriaceae Cultured From Retail Meat Products, Patients, and Porcine Excrement in China.
The study identified bla NDM-5, bla KPC-2, and mcr-1 as key resistance genes in carbapenem-resistant Enterobacteriaceae isolated from retail meat products, patients, and porcine excrement in China.
Specificities and Commonalities of Carbapenemase-Producing Escherichia coli Isolated in France from 2012 to 2015.
The study characterizes the diversity of carbapenemase-producing Escherichia coli isolates in France, identifying key resistance genes such as bla OXA-48, bla OXA-181, bla NDM-1, bla NDM-5, bla VIM-1, bla VIM-4, bla KPC-2, bla KPC-3, and mcr-9. It highlights the role of mutations in ftsI, ompC, gyrA, and parC in reducing susceptibility to β-lactams and fluoroquinolones.
Specificities and Commonalities of Carbapenemase-Producing Escherichia coli Isolated in France from 2012 to 2015.
The study characterizes the diversity of carbapenemase-producing Escherichia coli isolates in France, identifying key resistance genes such as bla OXA-48, bla OXA-181, bla NDM-1, bla NDM-5, bla VIM-1, bla VIM-4, bla KPC-2, bla KPC-3, and mcr-9. It highlights the role of mutations in ftsI, ompC, gyrA, and parC in reducing susceptibility to β-lactams and fluoroquinolones.
Emergence of a KPC-90 Variant that Confers Resistance to Ceftazidime-Avibactam in an ST463 Carbapenem-Resistant Pseudomonas aeruginosa Strain.
The study reports the emergence of a novel KPC-90 variant in a carbapenem-resistant Pseudomonas aeruginosa strain, which confers resistance to ceftazidime-avibactam through a 2-amino-acid insertion outside the KPC omega-loop region.
Emergence of a KPC-90 Variant that Confers Resistance to Ceftazidime-Avibactam in an ST463 Carbapenem-Resistant Pseudomonas aeruginosa Strain.
Emergence of a KPC-90 Variant that Confers Resistance to Ceftazidime-Avibactam in an ST463 Carbapenem-Resistant Pseudomonas aeruginosa Strain.
Breaking antimicrobial resistance by disrupting extracytoplasmic protein folding.
Disruption of DsbA-mediated disulfide bond formation incapacitates diverse β-lactamases and destabilizes mobile colistin resistance enzymes. Chemical inhibition of DsbA sensitizes multidrug-resistant clinical isolates to existing antibiotics.
Breaking antimicrobial resistance by disrupting extracytoplasmic protein folding.
Disruption of DsbA-mediated disulfide bond formation incapacitates diverse β-lactamases and destabilizes mobile colistin resistance enzymes. Chemical inhibition of DsbA sensitizes multidrug-resistant clinical isolates to existing antibiotics.
Distinctive Features of Ertapenem-Mono-Resistant Carbapenem-Resistant Enterobacterales in the United States: A Cohort Study.
The study found that ertapenem-mono-resistant CRE rarely have carbapenemase genes, with bla KPC being the most commonly detected carbapenemase gene. These isolates were less likely to have any carbapenemase compared to other CRE.
In vivo selection of KPC-94 and KPC-95 in Klebsiella pneumoniae isolates from patients treated with ceftazidime/avibactam.
In vivo selection of KPC-94 and KPC-95 in Klebsiella pneumoniae isolates from patients treated with ceftazidime/avibactam.
In vivo selection of KPC-94 and KPC-95 in Klebsiella pneumoniae isolates from patients treated with ceftazidime/avibactam.
In vivo selection of KPC-94 and KPC-95 in Klebsiella pneumoniae isolates from patients treated with ceftazidime/avibactam.
The Genomic Characterization of KPC-Producing Klebsiella pneumoniae from the ICU of a Teaching Hospital in Shanghai, China.
The study identified the carbapenemase gene blaKPC-2 and blaKPC-24, along with fosfomycin resistance gene fosA6, and various extended-spectrum beta-lactamase genes such as blaCTX-M-15, blaCTX-M-65, and blaCTX-M-27 in KPC-producing Klebsiella pneumoniae strains. Additionally, genes associated with fluoroquinolone, macrolide, aminoglycoside, and sulfonamide resistance were also characterized.
The Genomic Characterization of KPC-Producing Klebsiella pneumoniae from the ICU of a Teaching Hospital in Shanghai, China.
The study identified the carbapenemase gene blaKPC-2 and blaKPC-24, along with fosfomycin resistance gene fosA6, and various extended-spectrum beta-lactamase genes such as blaCTX-M-15, blaCTX-M-65, and blaCTX-M-27 in KPC-producing Klebsiella pneumoniae strains. Additionally, genes associated with fluoroquinolone, macrolide, aminoglycoside, and sulfonamide resistance were also characterized.
Plethora of Resistance Genes in Carbapenem-Resistant Gram-Negative Bacteria in Greece: No End to a Continuous Genetic Evolution.
The study identified a variety of resistance genes in carbapenem-resistant Gram-negative bacteria, including bla KPC, bla NDM, bla VIM, and others, highlighting the complex genetic diversity of these pathogens.
Emergence and Genomic Characterization of a KPC-2-, NDM-1-, and IMP-4-Producing Klebsiella michiganensis Isolate.
The study identifies a Klebsiella michiganensis isolate producing KPC-2, NDM-1, and IMP-4 carbapenemases, highlighting the emergence of multidrug-resistant strains and the potential for horizontal gene transfer.
Major Bloodstream Infection-Causing Bacterial Pathogens and Their Antimicrobial Resistance in South Korea, 2017-2019: Phase I Report From Kor-GLASS.
The study identified several AMR genes, including bla CTX-M–27, vanA, bla OXA–23, bla KPC, bla NDM–5, bla OXA–181, bla DHA–1, bla CMY–2, and mecA, which contribute to resistance against various antibiotics in bloodstream infection-causing pathogens in South Korea.
Major Bloodstream Infection-Causing Bacterial Pathogens and Their Antimicrobial Resistance in South Korea, 2017-2019: Phase I Report From Kor-GLASS.
The study identified several AMR genes, including bla CTX-M–27, vanA, bla OXA–23, bla KPC, bla NDM–5, bla OXA–181, bla DHA–1, bla CMY–2, and mecA, which contribute to resistance against various antibiotics in bloodstream infection-causing pathogens in South Korea.
Carbapenemase Production and Epidemiological Characteristics of Carbapenem-Resistant Klebsiella pneumoniae in Western Chongqing, China.
The study identified bla KPC-2, bla NDM, bla TEM, bla SHV, bla CTX-M-9, bla DHA, qnrS, and aac(6')-Ib-cr as prevalent resistance genes in carbapenem-resistant Klebsiella pneumoniae isolates in western Chongqing, China.
Isolation of a Ceftazidime-Avibactam-Resistant bla(KPC-71)-Positive Klebsiella pneumoniae Clinical Isolate.
A ceftazidime-avibactam-resistant K. pneumoniae isolate producing blaKPC-71 was identified. This variant shows reduced susceptibility to carbapenems compared to KPC-2.
Case report of enterobacter hormaechei in sheep with respiratory disease and death.
The study reports the first case of Enterobacter hormaechei causing respiratory disease and death in sheep, highlighting the presence of blaSHV and blaKPC genes conferring resistance to multiple beta-lactam antibiotics.
Faecal microbiota transplantation reduces amounts of antibiotic resistance genes in patients with multidrug-resistant organisms.
FMT reduces the expression of antibiotic resistance genes, particularly VanA and bla NDM, in patients colonized with multidrug-resistant organisms.
Impact of Ceftazidime-Avibactam Treatment in the Emergence of Novel KPC Variants in the ST307-Klebsiella pneumoniae High-Risk Clone and Consequences for Their Routine Detection.
The study identifies novel KPC variants (blaKPC-46, blaKPC-66, and blaKPC-92) in ST307-Klebsiella pneumoniae isolates that confer resistance to ceftazidime-avibactam.
Impact of Ceftazidime-Avibactam Treatment in the Emergence of Novel KPC Variants in the ST307-Klebsiella pneumoniae High-Risk Clone and Consequences for Their Routine Detection.
The study identifies novel KPC variants (blaKPC-46, blaKPC-66, and blaKPC-92) in ST307-Klebsiella pneumoniae isolates that confer resistance to ceftazidime-avibactam.
Impact of Ceftazidime-Avibactam Treatment in the Emergence of Novel KPC Variants in the ST307-Klebsiella pneumoniae High-Risk Clone and Consequences for Their Routine Detection.
The study identifies novel KPC variants (blaKPC-46, blaKPC-66, and blaKPC-92) in ST307-Klebsiella pneumoniae isolates that confer resistance to ceftazidime-avibactam.
Impact of Ceftazidime-Avibactam Treatment in the Emergence of Novel KPC Variants in the ST307-Klebsiella pneumoniae High-Risk Clone and Consequences for Their Routine Detection.
The study identifies novel KPC variants (blaKPC-46, blaKPC-66, and blaKPC-92) in ST307-Klebsiella pneumoniae isolates that confer resistance to ceftazidime-avibactam.
Impact of Ceftazidime-Avibactam Treatment in the Emergence of Novel KPC Variants in the ST307-Klebsiella pneumoniae High-Risk Clone and Consequences for Their Routine Detection.
Impact of Ceftazidime-Avibactam Treatment in the Emergence of Novel KPC Variants in the ST307-Klebsiella pneumoniae High-Risk Clone and Consequences for Their Routine Detection.
Impact of Ceftazidime-Avibactam Treatment in the Emergence of Novel KPC Variants in the ST307-Klebsiella pneumoniae High-Risk Clone and Consequences for Their Routine Detection.
Impact of Ceftazidime-Avibactam Treatment in the Emergence of Novel KPC Variants in the ST307-Klebsiella pneumoniae High-Risk Clone and Consequences for Their Routine Detection.
Impact of Ceftazidime-Avibactam Treatment in the Emergence of Novel KPC Variants in the ST307-Klebsiella pneumoniae High-Risk Clone and Consequences for Their Routine Detection.
Impact of Ceftazidime-Avibactam Treatment in the Emergence of Novel KPC Variants in the ST307-Klebsiella pneumoniae High-Risk Clone and Consequences for Their Routine Detection.
IS26 Veers Genomic Plasticity and Genetic Rearrangement toward Carbapenem Hyperresistance under Sublethal Antibiotics.
The study shows that IS26-mediated amplification of blaKPC-2 leads to carbapenem hyperresistance in E. coli. The amplification of blaKPC-2 was found to be mediated by IS26, and the copy number of blaKPC-2 was positively correlated with antibiotic concentration.
Antimicrobial resistance profile of extended-spectrum beta-lactamases, adenosine-monophosphate-cyclic, and carbapenemase-producing Gram-negative bacteria isolated from domestic animals.
The study identified ESBL-producing E. coli and K. pneumoniae, as well as KPC-producing K. pneumoniae, highlighting the presence of extended-spectrum beta-lactamases and carbapenemases in Gram-negative bacteria isolated from domestic animals.
Microbial Characteristics and Genomic Analysis of an ST11 Carbapenem-Resistant Klebsiella pneumoniae Strain Carrying bla (KPC-2) Conjugative Drug-Resistant Plasmid.
The study identifies the presence of the carbapenem resistance gene blaKPC-2 and the extended-spectrum beta-lactamase gene blaSHV-182 in an ST11 carbapenem-resistant Klebsiella pneumoniae strain. The blaKPC-2 gene was located on a conjugative plasmid, highlighting its potential for horizontal transfer.
Application of mNGS in the Etiological Analysis of Lower Respiratory Tract Infections and the Prediction of Drug Resistance.
The study identified several AMR genes using mNGS, including bla OXA-23, bla OXA-51, bla TEM, bla CTX-M, bla SHV, bla KPC, and mecA, which were associated with resistance to various antibiotics in different bacterial species.
A Genomic and Bioinformatics View of the Classification and Evolution of Morganella Species and Their Chromosomal Accessory Genetic Elements Harboring Antimicrobial Resistance Genes.
The study identified 88 acquired antimicrobial resistance genes (ARGs) in 166 Morganella isolates, with a focus on tetracycline, aminoglycoside, sulfonamide, trimethoprim, and beta-lactam resistance genes. Key ARGs included blaKPC-2, blaNDM-1, aacA4, aadA5, dfrA17, catB3, arr-3, blaOXA-1, aacA4cr, mph(A), rmtB, sul2, floR, qnrS1, tetA, and ermB.
First Description of Ceftazidime/Avibactam Resistance in a ST13 KPC-70-Producing Klebsiella pneumoniae Strain from Portugal.
The study reports the first description of ceftazidime/avibactam resistance in a ST13 KPC-70-producing Klebsiella pneumoniae strain from Portugal. The strain was found to harbor the blaKPC-70 gene, a variant of blaKPC-3 with two amino acid substitutions (D179Y and T263A), which conferred resistance to several β-lactam antibiotics.
Similarities in Virulence and Extended Spectrum Beta-Lactamase Gene Profiles among Cefotaxime-Resistant Escherichia coli Wastewater and Clinical Isolates.
The study identifies several beta-lactamase genes, including blaCTX-M-1, blaKPC-2, blaTEM-350, blaOXA-1, and blaCTX-M-15, as well as various aminoglycoside, macrolide, and tetracycline resistance genes in cefotaxime-resistant E. coli isolates from hospital and urban wastewater. Mutations in parC, parE, and gyrA contribute to fluoroquinolone resistance.
Evaluation of the VITEK2 AST-XN17 card for the detection of carbapenemase-producing Enterobacterales in isolates primarily producing metallo β-lactamase.
The study evaluates the effectiveness of the VITEK2 AST-XN17 card in detecting carbapenemase-producing Enterobacterales (CPE) and identifies several carbapenemase genes, including blaIMP-6, blaIMP-1, blaIMP-34, blaGES-4, and others, which confer resistance to carbapenems.
Activity of imipenem/relebactam on Klebsiella pneumoniae with different mechanisms of imipenem non-susceptibility.
Relebactam restored imipenem susceptibility in K. pneumoniae isolates with bla KPC and AmpC but not in those with MBLs. Impaired porin expression and efflux pump activity contributed to imipenem resistance.
Multidrug-resistant Klebsiella pneumoniae: a retrospective study in Manaus, Brazil.
The study identified multiple multidrug-resistant (MDR) and extensively drug-resistant (XDR) Klebsiella pneumoniae strains in Manaus, Brazil, harboring various beta-lactamase genes such as bla OXA-1-like, bla CTX-M-Gp1, and bla KPC. These strains exhibited resistance to multiple antibiotic classes, including carbapenems, cephalosporins, and penicillins.
A New Method Based on LAMP-CRISPR-Cas12a-Lateral Flow Immunochromatographic Strip for Detection.
The study presents a novel method combining LAMP-CRISPR-Cas12a with a lateral flow immunochromatographic strip for rapid detection of KPC and NDM carbapenemases in clinical samples.
Genetic and virulence characteristics of a Raoultella planticola isolate resistant to carbapenem and tigecycline.
The study identifies multiple antibiotic resistance genes in a Raoultella planticola isolate, including bla KPC-2, bla NDM-1, and tmexCD1-toprJ1, which confer resistance to carbapenems, cephalosporins, and tigecycline, respectively.
Genetic Characterization of Colistin-Resistant Salmonella enterica ST34 Co-Harbouring Plasmid-Borne mcr-1, bla (CTX-M-15) and bla (KPC-2) Recovered from a Paediatric Patient in Shenzhen, China.
The study identifies a colistin-resistant Salmonella enterica ST34 isolate carrying plasmid-borne mcr-1, bla(KPC-2), and bla(CTX-M-15) on an IncX4 plasmid, highlighting the potential for horizontal gene transfer and the emergence of multidrug-resistant superbugs.
Comparison of the Performance of Phenotypic Methods for the Detection of Carbapenem-Resistant Enterobacteriaceae (CRE) in Clinical Practice.
The study identified several carbapenemase genes, including bla KPC-2, bla NDM-1, bla NDM-4, bla NDM-5, bla IMP-4, bla IMP-8, and bla OXA-23, which are responsible for carbapenem resistance in Enterobacteriaceae. These genes were detected in various bacterial species, and their presence was validated through genetic methods.
High-Risk Clone of Klebsiella pneumoniae Co-Harbouring Class A and D Carbapenemases in Italy.
The study identified a high-risk clone of Klebsiella pneumoniae co-harboring class A (blaKPC) and D (blaOXA-48) carbapenemases, highlighting the emergence of multidrug-resistant strains in Italy.
Predictors and Outcomes of Healthcare-Associated Infections Caused by Carbapenem-Nonsusceptible Enterobacterales: A Parallel Matched Case-Control Study.
The study identified various carbapenemase genes including bla KPC-2, bla OXA-48, bla NDM-5, bla NDM-1, bla OXA-181, bla IMP-4, bla OXA-232, and bla NDM-7 as responsible for carbapenem resistance in Enterobacterales causing healthcare-associated infections in Singapore.
Virulence determinant and antimicrobial resistance traits of Emerging MDR Shiga toxigenic E. coli in diarrheic dogs.
The study identified several AMR genes in MDR Shiga toxigenic E. coli (STEC) isolated from diarrheic dogs in Egypt, including bla TEM, bla CTX-M, bla KPC, bla NDM-1, tet A, tet B, sul 1, and qnr A. These genes confer resistance to various antibiotics such as penicillins, cephalosporins, carbapenems, tetracyclines, sulfonamides, and quinolones.
Genomic Epidemiology of Carbapenemase-producing Klebsiella pneumoniae in China.
The study identifies blaKPC, blaNDM, and blaIMP genes as the primary carbapenemase genes in carbapenemase-producing Klebsiella pneumoniae (cpKP) isolates in China, highlighting their association with the spread of CG258 cpKP isolates. It also characterizes specific Inc groups of blaKPC-harboring plasmids that contribute to the resistance and competitive advantage of these isolates.
Genomic Epidemiology of Carbapenemase-producing Klebsiella pneumoniae in China.
The study identifies blaKPC, blaNDM, and blaIMP genes as the primary carbapenemase genes in carbapenemase-producing Klebsiella pneumoniae (cpKP) isolates in China, highlighting their association with the spread of CG258 cpKP isolates. It also characterizes specific Inc groups of blaKPC-harboring plasmids that contribute to the resistance and competitive advantage of these isolates.
Genomic Epidemiology of Carbapenemase-producing Klebsiella pneumoniae in China.
The study identifies blaKPC, blaNDM, and blaIMP genes as the primary carbapenemase genes in carbapenemase-producing Klebsiella pneumoniae (cpKP) isolates in China, highlighting their association with the spread of CG258 cpKP isolates. It also characterizes specific Inc groups of blaKPC-harboring plasmids that contribute to the resistance and competitive advantage of these isolates.
Comparing Long-Read Assemblers to Explore the Potential of a Sustainable Low-Cost, Low-Infrastructure Approach to Sequence Antimicrobial Resistant Bacteria With Oxford Nanopore Sequencing.
The study identifies the presence of the bla KPC gene in Klebsiella pneumoniae isolates using long-read sequencing, highlighting its role in carbapenem resistance.
Comparing Long-Read Assemblers to Explore the Potential of a Sustainable Low-Cost, Low-Infrastructure Approach to Sequence Antimicrobial Resistant Bacteria With Oxford Nanopore Sequencing.
The study identifies the presence of the bla KPC gene in Klebsiella pneumoniae isolates using long-read sequencing, highlighting its role in carbapenem resistance.
Emergence of Hypervirulent ST11-K64 Klebsiella pneumoniae Poses a Serious Clinical Threat in Older Patients.
The study identifies blaKPC-2 as the primary carbapenem resistance gene in CR-hvKP strains, highlighting its role in resistance to imipenem and meropenem.
Global population structure of the Serratia marcescens complex and identification of hospital-adapted lineages in the complex.
The study identified multiple antimicrobial resistance (AMR) genes and mutations in the Serratia marcescens complex, highlighting the presence of hospital-adapted lineages with a high prevalence of multidrug-resistant (MDR) strains. Key AMR genes include blaCTX-M, blaNDM, blaOXA, qnrS1, tet(A), aac(6')-Ib, mph(A), erm(B), aadA, floR, sul1, and dfrA12, which confer resistance to various antibiotics such as beta-lactams, fluoroquinolones, tetracyclines, aminoglycosides, macrolides, florfenicol, sulfonamides, and trimethoprim.
Metagenomic Approaches Reveal Strain Profiling and Genotyping of Klebsiella pneumoniae from Hospitalized Patients in China.
The study identified various antimicrobial resistance genes in Klebsiella pneumoniae strains, including blaKPC-2, blaKPC-3, blaSHV-11, CTX-M, qnrS1, and tet(A), which confer resistance to carbapenems, beta-lactams, fluoroquinolones, and tetracyclines.
Metagenomic Approaches Reveal Strain Profiling and Genotyping of Klebsiella pneumoniae from Hospitalized Patients in China.
The study identified various antimicrobial resistance genes in Klebsiella pneumoniae strains, including blaKPC-2, blaKPC-3, blaSHV-11, CTX-M, qnrS1, and tet(A), which confer resistance to carbapenems, beta-lactams, fluoroquinolones, and tetracyclines.
Co-Occurrence of Rare ArmA-, RmtB-, and KPC-2-Encoding Multidrug-Resistant Plasmids and Hypervirulence iuc Operon in ST11-KL47 Klebsiella pneumoniae.
The study identifies the co-occurrence of armA, rmtB, and blaKPC-2 genes in multidrug-resistant Klebsiella pneumoniae isolates, highlighting their role in resistance to aminoglycosides and carbapenems.
Surveillance of Antimicrobial Resistance in Hospital Wastewater: Identification of Carbapenemase-Producing Klebsiella spp.
The study identified bla KPC and bla OXA-48-like genes in carbapenem-resistant Klebsiella spp. isolates from hospital wastewater, highlighting the presence of carbapenemase-producing bacteria in wastewater treatment plants.
Whole Genome Sequence Analysis of Multidrug Resistant Escherichia coli and Klebsiella pneumoniae Strains in Kuwait.
The study identified multiple AMR genes in multidrug-resistant E. coli and K. pneumoniae isolates from Kuwait, including beta-lactamases (blaKPC-2, blaCTX-M-15, blaOXA-1, blaCMY-4, blaTEM), aminoglycoside-modifying enzymes (aac(3)-IIa, aph(6)-Id, aadA5), sulfonamide resistance genes (sul1, sul2), quinolone resistance genes (gyrA_D87N, qnrB1), and others. Colistin resistance was linked to the pmrB_R256G mutation.
Whole Genome Sequence Analysis of Multidrug Resistant Escherichia coli and Klebsiella pneumoniae Strains in Kuwait.
The study identified multiple AMR genes in multidrug-resistant E. coli and K. pneumoniae isolates from Kuwait, including beta-lactamases (blaKPC-2, blaCTX-M-15, blaOXA-1, blaCMY-4, blaTEM), aminoglycoside-modifying enzymes (aac(3)-IIa, aph(6)-Id, aadA5), sulfonamide resistance genes (sul1, sul2), quinolone resistance genes (gyrA_D87N, qnrB1), and others. Colistin resistance was linked to the pmrB_R256G mutation.
Increasing Trends of Association of 16S rRNA Methylases and Carbapenemases in Enterobacterales Clinical Isolates from Switzerland, 2017-2020.
The study identified a high prevalence of 16S rRNA methylases (ArmA, RmtF, RmtB, RmtC, RmtG) and carbapenemases (NDM-1, NDM-5, KPC-2, KPC-3, OXA-48, OXA-181, OXA-232, VIM-1, VIM-2) in carbapenem- and aminoglycoside-resistant Enterobacterales isolates from Switzerland, highlighting the increasing trends of their association.
Increasing Trends of Association of 16S rRNA Methylases and Carbapenemases in Enterobacterales Clinical Isolates from Switzerland, 2017-2020.
The study identified a high prevalence of 16S rRNA methylases (ArmA, RmtF, RmtB, RmtC, RmtG) and carbapenemases (NDM-1, NDM-5, KPC-2, KPC-3, OXA-48, OXA-181, OXA-232, VIM-1, VIM-2) in carbapenem- and aminoglycoside-resistant Enterobacterales isolates from Switzerland, highlighting the increasing trends of their association.
Structural Characterization of the D179N and D179Y Variants of KPC-2 beta-lactamase: Ω-Loop Destabilization as a Mechanism of Resistance to Ceftazidime-Avibactam.
The D179N and D179Y mutations in KPC-2 beta-lactamase confer resistance to ceftazidime-avibactam through destabilization of the Ω-loop, leading to reduced susceptibility.
Clonal spread of carbapenemase-producing Enterobacterales in a region, China.
The study identified blaKPC-2 and blaNDM as the most prevalent carbapenemase genes in carbapenem-resistant Enterobacterales (CRE) isolates, with blaKPC-2 being dominant in Klebsiella pneumoniae and blaNDM in various species. The clonal spread of ST11 KPC-2 Klebsiella pneumoniae was highlighted as a key contributor to the rise in CRE in the region.
Prolonged Outbreak of Carbapenem and Colistin-Resistant Klebsiella pneumoniae at a Large Tertiary Hospital in Brazil.
Inter-species geographic signatures for tracing horizontal gene transfer and long-term persistence of carbapenem resistance.
The study characterizes various carbapenem resistance genes such as blaKPC, blaNDM, blaOXA-48, blaVIM, blaIMP, blaGES, blaSIM, blaTEM, blaSHV, blaCTX-M, ampC, mecA, vanA, vanB, vanC, mcr-1, mcr-2, mcr-3, mcr-4, and mcr-5 in Enterobacterales and other bacterial species, highlighting their role in carbapenem resistance and horizontal gene transfer.
Inter-species geographic signatures for tracing horizontal gene transfer and long-term persistence of carbapenem resistance.
The study characterizes various carbapenem resistance genes such as blaKPC, blaNDM, blaOXA-48, blaVIM, blaIMP, blaGES, blaSIM, blaTEM, blaSHV, blaCTX-M, ampC, mecA, vanA, vanB, vanC, mcr-1, mcr-2, mcr-3, mcr-4, and mcr-5 in Enterobacterales and other bacterial species, highlighting their role in carbapenem resistance and horizontal gene transfer.
Inter-species geographic signatures for tracing horizontal gene transfer and long-term persistence of carbapenem resistance.
The study characterizes various carbapenem resistance genes such as blaKPC, blaNDM, blaOXA-48, blaVIM, blaIMP, blaGES, blaSIM, blaTEM, blaSHV, blaCTX-M, ampC, mecA, vanA, vanB, vanC, mcr-1, mcr-2, mcr-3, mcr-4, and mcr-5 in Enterobacterales and other bacterial species, highlighting their role in carbapenem resistance and horizontal gene transfer.
blaKPC-2-Encoding IncP-6 Plasmids in Citrobacter freundii and Klebsiella variicola Strains from Hospital Sewage in Japan.
The study identifies blaKPC-2 encoding IncP-6 plasmids in Citrobacter freundii and Klebsiella variicola strains from hospital sewage in Japan, highlighting the role of hospital sewage as a potential reservoir for carbapenem-resistant bacteria.
Escherichia marmotae-a Human Pathogen Easily Misidentified as Escherichia coli.
The study identified antimicrobial resistance genes in Escherichia marmotae, including bla KPC, bla CTX-M, bla TEM-1b, and tetA, which confer resistance to carbapenems, beta-lactams, and tetracyclines.
Population structure of blaKPC-harbouring IncN plasmids at a New York City medical centre and evidence for multi-species horizontal transmission.
The study characterizes blaKPC-harbouring IncN plasmids and identifies their role in carbapenem resistance among various Enterobacterales species, highlighting horizontal transmission.
Large-Scale Genomic Epidemiology of Klebsiella pneumoniae Identified Clone Divergence with Hypervirulent Plus Antimicrobial-Resistant Characteristics Causing Within-Ward Strain Transmissions.
The study identified clone divergence in Klebsiella pneumoniae, particularly highlighting the emergence of hypervirulent plus antimicrobial-resistant (hv+AMR) subclones. Key AMR genes such as bla KPC-2, bla CTX-M-65, and others were characterized, along with virulence factors like rmpA, rmpA2, and ybt.
Identification of a KPC Variant Conferring Resistance to Ceftazidime-Avibactam from ST11 Carbapenem-Resistant Klebsiella pneumoniae Strains.
A novel KPC variant, KPC-93, was identified in two clinical isolates of Klebsiella pneumoniae that conferred resistance to ceftazidime-avibactam. The variant had a five-amino-acid insertion between Ambler positions 267 and 268 in KPC-2 and showed increased resistance to ceftazidime-avibactam but decreased carbapenemase activity.
Identification of a KPC Variant Conferring Resistance to Ceftazidime-Avibactam from ST11 Carbapenem-Resistant Klebsiella pneumoniae Strains.
Identification of a KPC Variant Conferring Resistance to Ceftazidime-Avibactam from ST11 Carbapenem-Resistant Klebsiella pneumoniae Strains.
Increased bla(KPC) Copy Number and OmpK35 and OmpK36 Porins Disruption Mediated Resistance to Imipenem/Relebactam and Meropenem/Vaborbactam in a KPC-Producing Klebsiella pneumoniae Clinical Isolate.
The study identifies increased blaKPC copy number and truncation of OmpK35 and OmpK36 porins as mechanisms of resistance to Imipenem/Relebactam and Meropenem/Vaborbactam in a KPC-producing Klebsiella pneumoniae clinical isolate.
Prevalence and Molecular Typing of Carbapenemase-Producing Enterobacterales among Newborn Patients in Italy.
The study identified various carbapenemase genes, including bla NDM, bla KPC, bla VIM, and bla OXA-48, along with other resistance genes such as aac(6')-Ib3, aph(3')-VI, rmtC, bla CMY-6, and bla CTX-M-15, in carbapenemase-producing Enterobacterales isolated from newborn patients in Italy.
β-Lactam Antibiotics and beta-lactamase Enzymes Inhibitors, Part 2: Our Limited Resources.
The paper reviews β-lactam antibiotics and beta-lactamase enzymes inhibitors, focusing on the mechanisms of resistance mediated by beta-lactamases such as TEM-1, SHV-1, KPC-2, OXA-48, and NDM-1, and highlights the need for new inhibitors to combat carbapenem-resistant bacteria.
Detection of blaKPC and blaNDM carbapenemase genes among Klebsiella pneumoniae isolates in Addis Ababa, Ethiopia: Dominance of blaNDM.
The study identified blaNDM and blaKPC carbapenemase genes in Klebsiella pneumoniae isolates in Ethiopia, with blaNDM being the dominant gene.
Panorama of Bacterial Infections Caused by Epidemic Resistant Strains.
The paper reviews the characteristics of antibiotic-resistant strains such as MRSA, VRE, MDR TB, ESBL-producing Enterobacterales, KPC, and NDM, highlighting their resistance mechanisms and the challenges they pose to public health.
Impact of a Rapid Molecular Test for Klebsiella pneumoniae Carbapenemase and Ceftazidime-Avibactam Use on Outcomes After Bacteremia Caused by Carbapenem-Resistant Enterobacterales.
The study found that bla KPC PCR testing of positive blood cultures was associated with decreased time until appropriate therapy and decreased mortality for CRE bacteremia, and ceftazidime-avibactam is a reasonable first-line therapy for these infections.
Characteristics of ST11 KPC-2-producing carbapenem-resistant hypervirulent Klebsiella pneumoniae causing nosocomial infection in a Chinese hospital.
The study identified ST11 KPC-2-producing carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) as the main type causing nosocomial infections in a Chinese hospital. Key resistance genes included bla KPC-2, bla CTX-M-65, bla SHV-11, bla SHV-12, and bla TEM-1, which conferred resistance to various beta-lactam antibiotics.
In-Human Multiyear Evolution of Carbapenem-Resistant Klebsiella pneumoniae Causing Chronic Colonization and Intermittent Urinary Tract Infections: A Case Study.
The study identifies multiple AMR genes and mutations in CRKP strains from a patient with chronic colonization and intermittent UTIs, highlighting the evolution of carbapenem resistance, biofilm production, and carbon metabolism over 4.5 years.
In-Human Multiyear Evolution of Carbapenem-Resistant Klebsiella pneumoniae Causing Chronic Colonization and Intermittent Urinary Tract Infections: A Case Study.
The study identifies multiple AMR genes and mutations in CRKP strains from a patient with chronic colonization and intermittent UTIs, highlighting the evolution of carbapenem resistance, biofilm production, and carbon metabolism over 4.5 years.
bla(KPC-24)-Harboring Aeromonas veronii from the Hospital Sewage Samples in China.
The study reports the isolation of Aeromonas veronii strains carrying the blaKPC-24 gene, which confers resistance to several beta-lactam antibiotics. The gene was cloned and its resistance profile was analyzed.
Emergence of a Hypervirulent Tigecycline-Resistant Klebsiella pneumoniae Strain Co-producing bla (NDM-1) and bla (KPC-2) With an Uncommon Sequence Type ST464 in Southwestern China.
The study reports on a hypervirulent tigecycline-resistant Klebsiella pneumoniae strain (AHSWKP25) co-producing bla(NDM-1) and bla(KPC-2) with an unusual sequence type ST464 in Southwestern China. The strain exhibits extensive drug resistance, including resistance to tigecycline, and possesses mutations in genes related to efflux pump regulation and outer membrane porins.
Emergence of a Hypervirulent Tigecycline-Resistant Klebsiella pneumoniae Strain Co-producing bla (NDM-1) and bla (KPC-2) With an Uncommon Sequence Type ST464 in Southwestern China.
The study reports on a hypervirulent tigecycline-resistant Klebsiella pneumoniae strain (AHSWKP25) co-producing bla(NDM-1) and bla(KPC-2) with an unusual sequence type ST464 in Southwestern China. The strain exhibits extensive drug resistance, including resistance to tigecycline, and possesses mutations in genes related to efflux pump regulation and outer membrane porins.
An Outbreak of ST859-K19 Carbapenem-Resistant Hypervirulent Klebsiella pneumoniae in a Chinese Teaching Hospital.
The study reports an outbreak of ST859-K19 carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) in a Chinese hospital, identifying several resistance and virulence genes, including blaKPC-2, blaTEM-1B, blaSHV-187, rmtB, qnrS1, fosA6, and various virulence factors.
A Small KPC-2-Producing Plasmid in Klebsiella pneumoniae: Implications for Diversified Vehicles of Carbapenem Resistance.
The study identifies a small IncN-type plasmid, pK186_KPC, which carries the bla KPC-2 gene and confers resistance to multiple beta-lactam antibiotics in Klebsiella pneumoniae. The plasmid was found to be non-conjugative but may transfer at a low frequency with a helper plasmid.
Detection of carbapenemases bla(OXA48)-bla(KPC)-bla(NDM)-bla(VIM) and extended-spectrum-β-lactamase bla(OXA1)-bla(SHV)-bla(TEM) genes in Gram-negative bacterial isolates from ICU burns patients.
The study identified various carbapenemase and extended-spectrum beta-lactamase genes in Gram-negative bacterial isolates from ICU burns patients, highlighting the prevalence of resistance to carbapenems and beta-lactams.
Submarine Outfalls of Treated Wastewater Effluents are Sources of Extensively- and Multidrug-Resistant KPC- and OXA-48-Producing Enterobacteriaceae in Coastal Marine Environment.
The study identified extensively drug-resistant (XDR) and multidrug-resistant (MDR) KPC- and OXA-48-producing Enterobacteriaceae in coastal marine environments, highlighting the presence of various AMR genes including bla KPC-2, bla OXA-48, and others.
Genomic epidemiology and temperature dependency of hypermucoviscous Klebsiella pneumoniae in Japan.
The study identifies multiple AMR genes and mutations in hypermucoviscous Klebsiella pneumoniae isolates from Japan, highlighting the presence of ESBLs, carbapenemases, and other resistance determinants. It also reveals temperature-dependent variations in the HMV phenotype and the genetic diversity of the isolates.
Antimicrobial Resistance Patterns and Clonal Distribution of E. coli, Enterobacter spp. and Acinetobacter spp. Strains Isolated from Two Hospital Wastewater Plants.
The study identified blaKPC and blaCTX-M genes in E. coli strains, indicating resistance to carbapenems and cephalosporins, respectively. These genes were detected in both raw and treated hospital wastewater samples.
KPC-3-, GES-5-, and VIM-1-Producing Enterobacterales Isolated from Urban Ponds.
The study identified bla KPC-3, bla GES-5, and bla VIM genes in various Enterobacterales isolates from urban ponds, highlighting their role in carbapenem resistance.
KPC-3-, GES-5-, and VIM-1-Producing Enterobacterales Isolated from Urban Ponds.
The study identified bla KPC-3, bla GES-5, and bla VIM genes in various Enterobacterales isolates from urban ponds, highlighting their role in carbapenem resistance.
BioFire FilmArray Pneumonia Panel enhances detection of pathogens and antimicrobial resistance in lower respiratory tract specimens.
The BioFire FilmArray Pneumonia Panel detected 97 targets, including 84 bacteria and nine antimicrobial resistance markers, enhancing the detection of pathogens and antimicrobial resistance in lower respiratory tract specimens.
AMR GENE DETECTION WITH A SIMPLE MICROSCOPE AND SMARTPHONE IMAGING
The study presents a simple assay using CRISPR/Cas9 and DNA combing to detect β-lactamase genes (bla CTX-M and bla NDM) on bacterial plasmids, demonstrating the presence of these resistance genes through linearization of plasmids upon Cas9-mediated cutting.
Efficacy and In Vitro Activity of Novel Antibiotics for Infections With Carbapenem-Resistant Gram-Negative Pathogens.
The paper characterizes several carbapenemase genes, including KPC, NDM, VIM, and OXA-48-like enzymes, which confer resistance to carbapenems. It also identifies 16S-RMTase as a gene associated with aminoglycoside resistance.
Molecular Analysis With 16S rRNA PCR/Sanger Sequencing and Molecular Antibiogram Performed on DNA Extracted From Valve Improve Diagnosis and Targeted Therapy of Infective Endocarditis: A Prospective Study.
The study highlights the effectiveness of molecular analysis (MA) combined with molecular antibiogram in improving the diagnosis and targeted therapy of infective endocarditis (IE), particularly in blood culture-negative cases. It shows that molecular antibiogram has high concordance with traditional antimicrobial susceptibility testing (AST) and can identify resistance genes such as aadA1, aacC1, AAC(6)-Ib-cr, QnrS, CTX-M-1 Group, SHV, KPC, and tetA.
Carbapenem antibiotic stress increases bla(KPC) (-2) gene relative copy number and bacterial resistance levels of Klebsiella pneumoniae.
The study shows that carbapenem antibiotic stress increases the relative copy number of the bla(KPC)-2 gene in Klebsiella pneumoniae, which is associated with increased carbapenem resistance levels.
Prevalence of Carbapenem-Resistant Hypervirulent Klebsiella pneumoniae and Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae in China Determined via Mouse Lethality Tests.
The study identified two bla KPC-positive hypervirulent K. pneumoniae strains (JS184 and TZ20) through mouse lethality tests, highlighting the low prevalence of carbapenem-resistant hypervirulent K. pneumoniae in China.
Wastewater Surveillance Detected Carbapenemase Enzymes in Clinically Relevant Gram-Negative Bacteria in Helsinki, Finland; 2011-2012.
The study identified carbapenemase genes bla GES, bla KPC, and bla VIM in clinically relevant gram-negative bacteria in wastewater samples from Helsinki, Finland, highlighting the significance of wastewater surveillance in tracking antimicrobial resistance.
Outbreak of IncX8 Plasmid-Mediated KPC-3-Producing Enterobacterales Infection, China.
The study reports an outbreak of KPC-3-producing Enterobacterales in China, highlighting the role of IncX8 plasmids in the dissemination of carbapenem resistance.
Colonization of White-Tailed Deer (Odocoileus virginianus) from Urban and Suburban Environments with Cephalosporinase- and Carbapenemase-Producing Enterobacterales.
The study identified the presence of carbapenemase genes bla KPC-2 and bla NDM-5 in Enterobacterales isolated from white-tailed deer in urban and suburban environments, highlighting the role of wildlife in the dissemination of antimicrobial resistance.
KPC-3-Producing Klebsiella pneumoniae Sequence Type 392 from a Dog's Clinical Isolate in Portugal.
The study characterizes a KPC-3-producing Klebsiella pneumoniae ST392 isolate from a dog in Portugal, highlighting the presence of multiple AMR genes including blaKPC-3, aac(6')-lb-cr, tet(A), sul2, qnrB1, and OqxB/A, indicating multidrug resistance.
The Rapid Emergence of Ceftazidime-Avibactam Resistance Mediated by KPC Variants in Carbapenem-Resistant Klebsiella pneumoniae in Zhejiang Province, China.
The study identified KPC variants, including KPC-33 and KPC-93, as mediators of ceftazidime-avibactam resistance in carbapenem-resistant Klebsiella pneumoniae strains in Zhejiang Province, China. Additionally, mutations in porin genes ompK35 and ompK36 were associated with resistance.
The Rapid Emergence of Ceftazidime-Avibactam Resistance Mediated by KPC Variants in Carbapenem-Resistant Klebsiella pneumoniae in Zhejiang Province, China.
The study identified KPC variants, including KPC-33 and KPC-93, as mediators of ceftazidime-avibactam resistance in carbapenem-resistant Klebsiella pneumoniae strains in Zhejiang Province, China. Additionally, mutations in porin genes ompK35 and ompK36 were associated with resistance.
The Rapid Emergence of Ceftazidime-Avibactam Resistance Mediated by KPC Variants in Carbapenem-Resistant Klebsiella pneumoniae in Zhejiang Province, China.
The study identified KPC variants, including KPC-33 and KPC-93, as mediators of ceftazidime-avibactam resistance in carbapenem-resistant Klebsiella pneumoniae strains in Zhejiang Province, China. Additionally, mutations in porin genes ompK35 and ompK36 were associated with resistance.
The Rapid Emergence of Ceftazidime-Avibactam Resistance Mediated by KPC Variants in Carbapenem-Resistant Klebsiella pneumoniae in Zhejiang Province, China.
The study identified KPC variants, including KPC-33 and KPC-93, as mediators of ceftazidime-avibactam resistance in carbapenem-resistant Klebsiella pneumoniae strains in Zhejiang Province, China. Additionally, mutations in porin genes ompK35 and ompK36 were associated with resistance.
Nosocomial Outbreak of Extensively Drug-Resistant (Polymyxin B and Carbapenem) Klebsiella pneumoniae in a Collapsed University Hospital Due to COVID-19 Pandemic.
The study identifies blaKPC-2 as a carbapenem resistance gene and mgrB gene mutations as a cause of polymyxin B resistance in extensively drug-resistant Klebsiella pneumoniae during a hospital outbreak linked to the COVID-19 pandemic.
Clinical and Molecular Analysis of ST11-K47 Carbapenem-Resistant Hypervirulent Klebsiella pneumoniae: A Strain Causing Liver Abscess.
The study identified a rare ST11-K47 carbapenem-resistant hypervirulent Klebsiella pneumoniae strain (HvKp-su1) carrying multiple resistance genes, including bla KPC-2, bla TEM-1, bla SHV-55, and bla CTX-M-65, highlighting the emergence of multidrug-resistant and hypervirulent strains.
Association of Carbapenemase-Producing Enterobacterales Detected in Stream and Clinical Samples.
The study identified carbapenemase genes such as bla KPC-2, bla KPC-3, bla NDM-1, bla NDM-5, bla GES-5, bla GES-6, and bla OXA-48 in carbapenem-resistant Enterobacterales (CRE) isolates from streams and clinical samples in Korea. These genes were associated with resistance to carbapenems, and certain sequence types (STs) were shared between environmental and clinical isolates.
Association of Carbapenemase-Producing Enterobacterales Detected in Stream and Clinical Samples.
The study identified carbapenemase genes such as bla KPC-2, bla KPC-3, bla NDM-1, bla NDM-5, bla GES-5, bla GES-6, and bla OXA-48 in carbapenem-resistant Enterobacterales (CRE) isolates from streams and clinical samples in Korea. These genes were associated with resistance to carbapenems, and certain sequence types (STs) were shared between environmental and clinical isolates.
Retail chicken giblets contaminated with extended-spectrum cephalosporin- and carbapenem-resistant Salmonella enterica carrying blaCMY-2.
The study identified extended-spectrum cephalosporin- and carbapenem-resistant Salmonella enterica in retail chicken giblets, water tanks, and workers, carrying blaTEM, blaSHV, blaCMY-2, blaOXA-1, and blaKPC genes.
Occurrence of antibiotics and bacterial resistance genes in wastewater: resistance mechanisms and antimicrobial resistance control approaches.
The study identifies several AMR genes and mutations associated with resistance to various antibiotics in wastewater environments, highlighting the role of these genes in the spread of antimicrobial resistance.
Identification of a Novel Ceftazidime-Avibactam-Resistant KPC-2 Variant, KPC-123, in Citrobacter koseri Following Ceftazidime-Avibactam Treatment.
The study identifies a novel KPC-2 variant, KPC-123, in Citrobacter koseri that exhibits resistance to ceftazidime and ceftazidime/avibactam.
Identification of a Novel Ceftazidime-Avibactam-Resistant KPC-2 Variant, KPC-123, in Citrobacter koseri Following Ceftazidime-Avibactam Treatment.
Identification of a Novel Ceftazidime-Avibactam-Resistant KPC-2 Variant, KPC-123, in Citrobacter koseri Following Ceftazidime-Avibactam Treatment.
Genomic surveillance for multidrug-resistant or hypervirulent Klebsiella pneumoniae among United States bloodstream isolates.
The study identified various antimicrobial resistance genes and mutations in Klebsiella pneumoniae bloodstream isolates, highlighting the presence of multidrug-resistant and hypervirulent strains in the United States.
Using Targeted Liquid Chromatography-Tandem Mass Spectrometry to Rapidly Detect β-Lactam, Aminoglycoside, and Fluoroquinolone Resistance Mechanisms in Blood Cultures Growing E. coli or K. pneumoniae.
The study developed and validated a targeted LC-MS/MS assay for the rapid detection of β-lactam, aminoglycoside, and fluoroquinolone resistance mechanisms in blood cultures growing E. coli or K. pneumoniae. The assay successfully detected various resistance genes including β-lactamases (SHV, TEM, CTX-M-1-like, OXA-1, CMY-2-like, cAmpC, KPC, OXA-48, NDM, VIM), aminoglycoside-modifying enzymes (AAC(3)-Ia, AAC(3)-II, AAC(3)-IV, AAC(3)-VI, AAC(6′)-Ib, ANT(2′′)-I, APH(3′)-VI), 16S-RMTases (ArmA, RmtB, RmtC, RmtF), and quinolone resistance mechanisms (QnrA, QnrB, AAC(6′)-Ib-cr, and wildtype QRDR of GyrA).
Epidemiology and clinical characteristics of infection/colonization due to carbapenemase-producing Enterobacterales in neonatal patients.
The study identified blaNDM-1, blaKPC-2, and blaIMP-4 as the primary carbapenemase genes responsible for carbapenem resistance in Enterobacterales isolates from neonatal patients. These genes were predominantly found in Klebsiella pneumoniae and Escherichia coli, contributing to high rates of rectal colonization and infections.
Molecular epidemiological characteristics of carbapenem-resistant Klebsiella pneumoniae among children in China.
The study identified KPC-2, NDM-1, and IPM-4 carbapenemase genes as the primary contributors to carbapenem resistance in Klebsiella pneumoniae isolates from children in China. Additionally, qacE and cepA genes were found to confer resistance to disinfectants.
Prevalence of hypervirulent and carbapenem-resistant Klebsiella pneumoniae under divergent evolutionary patterns.
The study identifies rfaH and wcaJ mutations as contributing to carbapenem resistance and reduced capsule production in 'CR-hvKp' strains, highlighting the complex interplay between virulence and antibiotic resistance in hypervirulent carbapenem-resistant Klebsiella pneumoniae (hv-CRKP).
Direct Colorimetry of Imipenem Decomposition as a Novel Cost-Effective Method for Detecting Carbapenemase-Producing Enterobacteria.
The study presents a novel, cost-effective method for detecting carbapenemase-producing enterobacteria (CPE) using direct colorimetry of imipenem decomposition. The method relies on the specific color change induced by carbapenemases, particularly NDM-1, NMC-A, OXA-48, VIM-1, IMP-4, and KPC-2, which were experimentally validated for their ability to hydrolyze imipenem and produce a measurable yellow color. The method demonstrated high sensitivity (98%) and specificity (100%).
Genomic Characterization of a Multidrug-Resistant Aeromonas caviae Isolate Carrying a Novel bla(KPC-2)-Harbouring Plasmid and an IMP-4-Encoding Phage-like Plasmid.
The study characterizes a multidrug-resistant Aeromonas caviae isolate carrying a novel bla(KPC-2)-harboring plasmid and an IMP-4-encoding phage-like plasmid, highlighting the role of mobile genetic elements in the dissemination of antimicrobial resistance genes.
Carbapenemase-Encoding Gene Copy Number Estimator (CCNE): a Tool for Carbapenemase Gene Copy Number Estimation.
The study introduces CCNE, a bioinformatics tool for estimating carbapenemase gene copy numbers, showing that higher bla KPC copy numbers correlate with increased carbapenem MICs and reduced susceptibility to ceftazidime-avibactam.
Transmission of Nonconjugative Virulence or Resistance Plasmids Mediated by a Self-Transferable IncN3 Plasmid from Carbapenem-Resistant Klebsiella pneumoniae.
The study identifies a self-transferable IncN3 plasmid that facilitates the transmission of nonconjugative virulence and resistance plasmids in carbapenem-resistant Klebsiella pneumoniae. The IncN3 plasmid was shown to mobilize nonconjugative plasmids through hybrid formation or cotransfer, highlighting its role in the spread of antimicrobial resistance and virulence traits.
The Gastrointestinal Load of Carbapenem-Resistant Enterobacteriacea Is Associated With the Transition From Colonization to Infection by Klebsiella pneumoniae Isolates Harboring the bla(KPC) Gene.
The study identifies blaKPC as a key gene associated with carbapenem resistance in Klebsiella pneumoniae isolates, highlighting its role in the transition from colonization to infection.
Excreted Antibiotics May Be Key to Emergence of Increasingly Efficient Antibiotic Resistance in Food Animal Production.
The study shows that bla CTX-M-15 provides a significant fitness advantage over bla CMY-2 and bla KPC-3 in high concentrations of ampicillin and ceftiofur, suggesting that excreted antibiotics play a crucial role in the emergence of more efficient antibiotic resistance genes in food animal production.
Dominant Carbapenemase-Encoding Plasmids in Clinical Enterobacterales Isolates and Hypervirulent Klebsiella pneumoniae, Singapore.
The study identifies pKPC2 and pNDM1 as the two dominant carbapenemase-encoding plasmids in clinical Enterobacterales isolates and hypervirulent Klebsiella pneumoniae in Singapore. pKPC2 exhibits higher conjugation frequency and stability compared to pNDM1.
QM/MM Simulations Reveal the Determinants of Carbapenemase Activity in Class A β-Lactamases.
The study identifies key structural and dynamic factors influencing carbapenemase activity in class A β-lactamases through QM/MM simulations, highlighting the roles of the 6α-1R-hydroxyethyl group, Asn132, and the disulfide bridge in determining carbapenem hydrolysis efficiency.
Molecular mechanisms underlying bacterial resistance to ceftazidime/avibactam.
The study identifies various mutations in blaKPC-2, blaKPC-3, blaCTX-M-14, blaCTX-M-15, blaSHV-1, and blaAmpC genes that confer resistance to ceftazidime/avibactam. These mutations include amino acid substitutions and insertions/deletions affecting the enzyme's affinity for CAZ and AVI, leading to increased MIC values and resistance.
Molecular mechanisms underlying bacterial resistance to ceftazidime/avibactam.
The study identifies various mutations in blaKPC-2, blaKPC-3, blaCTX-M-14, blaCTX-M-15, blaSHV-1, and blaAmpC genes that confer resistance to ceftazidime/avibactam. These mutations include amino acid substitutions and insertions/deletions affecting the enzyme's affinity for CAZ and AVI, leading to increased MIC values and resistance.
Increased Expression and Amplification of bla(KPC-2) Contributes to Resistance to Ceftazidime/Avibactam in a Sequence Type 11 Carbapenem-Resistant Klebsiella pneumoniae Strain.
A Selective Medium for Screening Ceftazidime/Avibactam Resistance in Carbapenem-Resistant Enterobacterales.
The study developed an SS CZA medium for screening CZA-resistant CRE isolates, demonstrating 100% sensitivity and specificity. The medium effectively distinguishes between CZA-resistant and -susceptible strains, with resistance determinants including blaNDM, blaKPC, blaCTX-M, blaTEM, blaSHV, blaOXA-23, blaIMP, and ampC.
A novel, magnetic bead-based extraction method for the isolation of antimicrobial resistance genes with a case study in river water in Malawi.
The study presents a novel, magnetic bead-based extraction method called MagnaExtract for isolating antimicrobial resistance genes (ARGs) from river water. The method showed comparable or better performance than commercial kits in terms of DNA yield and detection of ARGs such as bla CTXM-1, bla CTXM-9, bla SHV, bla OXA-48, bla IMP, and bla KPC.
Ceftazidime-avibactam based combinations against carbapenemase producing Klebsiella pneumoniae harboring hypervirulence plasmids.
The study identified several AMR genes, including bla KPC-2, bla OXA-48, and bla NDM-1, in carbapenem-resistant K. pneumoniae isolates. Additionally, the gene ompW was found to be significantly downregulated, potentially affecting antibiotic resistance.
Genomic epidemiological analysis of Klebsiella pneumoniae from Portuguese hospitals reveals insights into circulating antimicrobial resistance.
The study identifies bla KPC-3 and bla CTX-M-15 as the primary carbapenem and extended-spectrum beta-lactamase resistance genes, respectively, in Klebsiella pneumoniae isolates from Portugal. Other notable AMR genes include aac(6')-Ib-cr, qnrB, aac(3)-II, APH(3')-I, ANT(3'')-I, and APH(6)-I, which contribute to resistance against fluoroquinolones and aminoglycosides.
Detecting KPC-2 and NDM-1 Coexpression in Klebsiella pneumoniae Complex from Human and Animal Hosts in South America.
The study identifies the coexpression of KPC-2 and NDM-1 carbapenemases in members of the K. pneumoniae complex from human and animal hosts in South America, highlighting the need for improved detection methods.
Detecting KPC-2 and NDM-1 Coexpression in Klebsiella pneumoniae Complex from Human and Animal Hosts in South America.
Detecting KPC-2 and NDM-1 Coexpression in Klebsiella pneumoniae Complex from Human and Animal Hosts in South America.
Klebsiella pneumoniae Carbapenemase Variants Resistant to Ceftazidime-Avibactam: an Evolutionary Overview.
Klebsiella pneumoniae Carbapenemase Variants Resistant to Ceftazidime-Avibactam: an Evolutionary Overview.
Klebsiella pneumoniae Carbapenemase Variants Resistant to Ceftazidime-Avibactam: an Evolutionary Overview.
Klebsiella pneumoniae Carbapenemase Variants Resistant to Ceftazidime-Avibactam: an Evolutionary Overview.
Klebsiella pneumoniae Carbapenemase Variants Resistant to Ceftazidime-Avibactam: an Evolutionary Overview.
Klebsiella pneumoniae Carbapenemase Variants Resistant to Ceftazidime-Avibactam: an Evolutionary Overview.
Klebsiella pneumoniae Carbapenemase Variants Resistant to Ceftazidime-Avibactam: an Evolutionary Overview.
Klebsiella pneumoniae Carbapenemase Variants Resistant to Ceftazidime-Avibactam: an Evolutionary Overview.
Klebsiella pneumoniae Carbapenemase Variants Resistant to Ceftazidime-Avibactam: an Evolutionary Overview.
Klebsiella pneumoniae Carbapenemase Variants Resistant to Ceftazidime-Avibactam: an Evolutionary Overview.
Klebsiella pneumoniae Carbapenemase Variants Resistant to Ceftazidime-Avibactam: an Evolutionary Overview.
Klebsiella pneumoniae Carbapenemase Variants Resistant to Ceftazidime-Avibactam: an Evolutionary Overview.
Development of Microfluidic Chip-Based Loop-Mediated Isothermal Amplification (LAMP) Method for Detection of Carbapenemase Producing Bacteria.
The study developed a microfluidic chip-based LAMP method for the detection of carbapenemase-producing organisms (CPO) and evaluated its performance in identifying carbapenemase genes such as bla KPC, bla NDM, bla VIM, bla IMP, bla oprD2, bla OXA-23, bla OXA-48, and bla OXA-58.
Cooccurrence of Antibiotic Resistance and Hypervirulence in High-Risk Carbapenem-Resistant K14.K64 and Wzi209 Klebsiella pneumoniae Strains Driven by Plasmids and Their Derivatives.
The study identifies the blaKPC gene as a critical factor in carbapenem resistance in Klebsiella pneumoniae strains, highlighting the role of plasmids in the dissemination of antibiotic resistance and hypervirulence.
Diversity of Ceftazidime-Avibactam Resistance Mechanism in KPC2-Producing Klebsiella pneumoniae Under Antibiotic Selection Pressure.
Diversity of Ceftazidime-Avibactam Resistance Mechanism in KPC2-Producing Klebsiella pneumoniae Under Antibiotic Selection Pressure.
Diversity of Ceftazidime-Avibactam Resistance Mechanism in KPC2-Producing Klebsiella pneumoniae Under Antibiotic Selection Pressure.
Diversity of Ceftazidime-Avibactam Resistance Mechanism in KPC2-Producing Klebsiella pneumoniae Under Antibiotic Selection Pressure.
Diversity of Ceftazidime-Avibactam Resistance Mechanism in KPC2-Producing Klebsiella pneumoniae Under Antibiotic Selection Pressure.
Diversity of Ceftazidime-Avibactam Resistance Mechanism in KPC2-Producing Klebsiella pneumoniae Under Antibiotic Selection Pressure.
Molecular and clinical characterization of hypervirulent Klebsiella pneumoniae isolates from individuals with urinary tract infections.
The study identified the blaKPC-2 gene as a major contributor to carbapenem resistance in hypervirulent Klebsiella pneumoniae (hvKP) isolates from urinary tract infections. The gene was successfully transferred via conjugation and was associated with high resistance rates to carbapenems.
Fingolimod Promotes Antibacterial Effect of Doripenem against Carbapenem-Resistant Escherichia coli.
The study identified the blaKPC gene as responsible for carbapenem resistance in E. coli and demonstrated that fingolimod enhances the antibacterial effect of doripenem against carbapenem-resistant E. coli.
Systematic Analysis of Mobile Genetic Elements Mediating β-Lactamase Gene Amplification in Noncarbapenemase-Producing Carbapenem-Resistant Enterobacterales Bloodstream Infections.
The study identifies bla CTX-M-15, bla OXA-1, bla CTX-M-1, bla TEM-1, and bla CMY as key β-lactamase genes contributing to carbapenem resistance in noncarbapenemase-producing Enterobacterales (non-CP-CRE) bloodstream infections, with gene amplification mediated by IS 26 and IS Ecp1 elements.
Imipenem-Relebactam Susceptibility in Enterobacterales Isolates Recovered from ICU Patients from Spain and Portugal (SUPERIOR and STEP Studies).
The study identified several carbapenemase genes, including blaOXA-48, blaKPC-3, blaOXA-181, blaNDM-1, and blaVIM-2, which confer resistance to carbapenems in Enterobacterales isolates from ICU patients in Spain and Portugal. Additionally, other beta-lactamase genes such as ctxM-15, shv-like, and oxa-1 were found to contribute to resistance against cephalosporins and penicillins. The mobile colistin resistance gene mcr-9.1 was also detected in an Enterobacter hormaechei isolate.
Multidrug-Resistant Gram-Negative Bacteria in Burn Patients.
The study identified blaKPC-31, a carbapenemase gene, in Citrobacter freundii isolates from burn patients, which conferred resistance to ceftazidime-avibactam. The gene was located on two distinct plasmids, and its removal restored susceptibility to the antibiotic.
Whole-Genome Sequencing Reveals Diversity of Carbapenem-Resistant Pseudomonas aeruginosa Collected through CDC's Emerging Infections Program, United States, 2016-2018.
Whole-Genome Sequencing Reveals Diversity of Carbapenem-Resistant Pseudomonas aeruginosa Collected through CDC's Emerging Infections Program, United States, 2016-2018.
Genomic Surveillance of Carbapenem-Resistant Klebsiella pneumoniae from a Major Public Health Hospital in Singapore.
The study identified multiple carbapenemase genes, including blaKPC-2, blaOXA-48-like, and blaNDM, along with 16S rRNA methyltransferases (armA, rmtF, rmtB) and extended-spectrum beta-lactamase blaCTX-M-15, contributing to carbapenem and aminoglycoside resistance in Klebsiella pneumoniae isolates from Singapore.
The Molecular Epidemiology of Prevalent Klebsiella pneumoniae Strains and Humoral Antibody Responses against Carbapenem-Resistant K. pneumoniae Infections among Pediatric Patients in Shanghai.
The study identified the prevalence of carbapenem-resistant Klebsiella pneumoniae (CRKP) strains among pediatric patients in Shanghai, highlighting the dominance of the blaKPC gene as the primary carbapenemase responsible for resistance. Other carbapenemase genes such as blaNDM, blaIMP, and blaOXA-48 were also detected. Additionally, the study found that CRKP strains exhibited high resistance to beta-lactam antibiotics.
Broad-Spectrum Inhibitors against Class A, B, and C Type β-Lactamases to Block the Hydrolysis against Antibiotics: Kinetics and Structural Characterization.
The study identifies three novel broad-spectrum inhibitors (D63, D2148, and D2573) effective against class A, B, and C β-lactamases, demonstrating reduced MIC and improved enzyme inhibition compared to existing inhibitors like avibactam.
A Closer Look at the Laboratory Impact of Utilizing ePlex Blood Culture Identification Panels: a Workflow Analysis Using Rapid Molecular Detection for Positive Blood Cultures.
The ePlex BCID panels accurately detected 5 pan targets and 42 antimicrobial resistance gene markers, including 31 mecA, 4 vanA, 6 CTX-M, and 1 KPC gene.
Hitherto-Unnoticed Self-Transmissible Plasmids Widely Distributed among Different Environments in Japan.
The study identified new subgroups of IncP/P-1 and PromA plasmids, including antibiotic resistance genes such as blaIMP-1, blaKPC-2, mcr-1, and various tetracycline and aminoglycoside resistance genes.
Genomic characteristics of clinical multidrug-resistant Proteus isolates from a tertiary care hospital in southwest China.
The study identifies multiple AMR genes in clinical multidrug-resistant Proteus isolates, including bla CTX-M-65, bla OXA-1, bla KPC-2, bla NDM-1, and others, highlighting the genetic diversity of mobile genetic elements carrying resistance genes.
Multidrug-Resistant Bacteria: Their Mechanism of Action and Prophylaxis.
The paper reviews the mechanisms of multidrug resistance in bacteria, focusing on resistance mechanisms such as beta-lactamases, vancomycin resistance genes, and other resistance determinants in both Gram-positive and Gram-negative bacteria.
Molecular phenotyping approaches for the detection and monitoring of carbapenem-resistant Enterobacteriaceae by mass spectrometry.
The study discusses molecular phenotyping approaches using mass spectrometry for detecting and monitoring carbapenem-resistant Enterobacteriaceae, highlighting the role of carbapenemases like KPC-2 in resistance mechanisms.
Complete Genome Sequence of a Multidrug-Resistant Klebsiella pneumoniae Strain Carrying bla(OXA181) and bla(KPC-125) Carbapenemase.
Complete Genome Sequence of a Multidrug-Resistant Klebsiella pneumoniae Strain Carrying bla(OXA181) and bla(KPC-125) Carbapenemase.
Acquisition of resistance to ceftazidime-avibactam during infection treatment in Pseudomonas aeruginosa through D179Y mutation in one of two bla(KPC-2) gene copies without losing carbapenem resistance.
The study characterizes the acquisition of ceftazidime/avibactam resistance in Pseudomonas aeruginosa through a D179Y mutation in one of two bla(KPC-2) gene copies, without loss of carbapenem resistance.
Acquisition of resistance to ceftazidime-avibactam during infection treatment in Pseudomonas aeruginosa through D179Y mutation in one of two bla(KPC-2) gene copies without losing carbapenem resistance.
The study characterizes the acquisition of ceftazidime/avibactam resistance in Pseudomonas aeruginosa through a D179Y mutation in one of two bla(KPC-2) gene copies, without loss of carbapenem resistance.
Resistance Phenotype and Molecular Epidemiology of Carbapenem-Resistant Klebsiella pneumoniae Isolated from Nanjing Children's Hospital in Jiangsu Province, China.
The study identified several carbapenemase genes, including blaKPC-2, blaNDM-1, blaNDM-5, blaIMP-8, and blaOXA-181, as well as other resistance genes such as blaCTX-M, blaSHV-2, APH(3)-IB, APH(6)-ID, FosA5, tetC, qnrB1, and tetA, contributing to multidrug resistance in CRKP strains isolated from children in Nanjing, China.
Epidemiology, Mechanisms of Resistance and Treatment Algorithm for Infections Due to Carbapenem-Resistant Gram-Negative Bacteria: An Expert Panel Opinion.
The paper discusses the mechanisms of resistance in carbapenem-resistant Gram-negative bacteria, highlighting the role of various beta-lactamases such as blaOXA-51, blaOXA-23, blaOXA-24, blaOXA-48, blaKPC, blaNDM, blaVIM, and blaIMP, as well as aminoglycoside modifying enzymes like aac(6')-Ib and aadA, and quinolone resistance genes such as qnrS1.
Evaluation of the EasyScreen™ ESBL/CPO Detection Kit for the Detection of ß-Lactam Resistance Genes.
The EasyScreen™ ESBL/CPO Detection Kit effectively detects various β-lactam resistance genes, including bla VIM, bla NDM, bla IMP, bla OXA-48, bla KPC, bla OXA-23, bla OXA-51, bla SME, bla IMI, bla GES, bla TEM, bla SHV, bla CTX-M, bla CMY, bla DHA, and the mcr-1 gene, demonstrating high sensitivity and specificity for carbapenemase and ESBL detection in Enterobacterales, Pseudomonas spp., and Acinetobacter spp.
Phage-Plasmids Spread Antibiotic Resistance Genes through Infection and Lysogenic Conversion.
Phage-plasmids (P-Ps) carry a variety of antibiotic resistance genes (ARGs), including beta-lactamases, aminoglycoside-modifying enzymes, and carbapenemases. These genes are often located in integrons and are associated with transposable elements. P-Ps can be induced by mitomycin C and can transfer resistance genes through lysogenic conversion.
Emergence of Extensively Drug-Resistant ST170 Citrobacter portucalensis with Plasmids pK218-KPC, pK218-NDM, and pK218-SHV from a Tertiary Hospital, China.
The study identifies the emergence of an extensively drug-resistant Citrobacter portucalensis strain, K218, which carries multiple resistance genes including bla KPC-2 and bla NDM-1, contributing to its multidrug-resistant phenotype.
Clinical Efficacy, Antibiotic Resistance Genes, Virulence Factors and Outcome of Hospital-Acquired Pneumonia Induced by Klebsiella pneumoniae Carbapenemase 2-Producing with Tigecycline Treatment in the ICU.
The study identified several antibiotic resistance genes, including blaKPC-2, blaSHV, blaCTX-M, blaTEM, and aac3iia, which are associated with resistance to various antibiotics. Additionally, virulence factors such as AREO-iutA and Capsule-wzc were found to be independently associated with mortality in patients with KPC-KP infections.
Transmission of Carbapenem-Resistant Klebsiella pneumoniae in US Hospitals
The study identified bla KPC-2 and bla KPC-3 as the most common carbapenemase genes in carbapenem-resistant Klebsiella pneumoniae isolates, highlighting their role in resistance.
Transmission of Carbapenem-Resistant Klebsiella pneumoniae in US Hospitals
The study identified bla KPC-2 and bla KPC-3 as the most common carbapenemase genes in carbapenem-resistant Klebsiella pneumoniae isolates, highlighting their role in resistance.
Clinical and Molecular Characterizations of Carbapenem-Resistant Klebsiella pneumoniae Causing Bloodstream Infection in a Chinese Hospital.
The study identified blaKPC-2, blaNDM-1, and blaNDM-5 as the primary carbapenem resistance genes in CRKP strains. Additionally, virulence genes such as rmpA2, iucABCD, iutA, and repB were found to be associated with ST11-KL64 strains.
Prevalence of carbapenemase genes among carbapenem-nonsusceptible Enterobacterales collected in US hospitals in a five-year period and activity of ceftazidime/avibactam and comparator agents.
The study identified several carbapenemase genes, including bla KPC, bla OXA-181, bla NDM-1, bla NDM-5, bla VIM-1, and bla IMP-27, in carbapenem-nonsusceptible Enterobacterales isolates. New KPC variants, such as bla KPC-58, were characterized and showed resistance to ceftazidime/avibactam and low meropenem MIC values.
Prevalence of carbapenemase genes among carbapenem-nonsusceptible Enterobacterales collected in US hospitals in a five-year period and activity of ceftazidime/avibactam and comparator agents.
The study identified several carbapenemase genes, including bla KPC, bla OXA-181, bla NDM-1, bla NDM-5, bla VIM-1, and bla IMP-27, in carbapenem-nonsusceptible Enterobacterales isolates. New KPC variants, such as bla KPC-58, were characterized and showed resistance to ceftazidime/avibactam and low meropenem MIC values.
Prevalence of carbapenemase genes among carbapenem-nonsusceptible Enterobacterales collected in US hospitals in a five-year period and activity of ceftazidime/avibactam and comparator agents.
The study identified several carbapenemase genes, including bla KPC, bla OXA-181, bla NDM-1, bla NDM-5, bla VIM-1, and bla IMP-27, in carbapenem-nonsusceptible Enterobacterales isolates. New KPC variants, such as bla KPC-58, were characterized and showed resistance to ceftazidime/avibactam and low meropenem MIC values.
Prevalence of carbapenemase genes among carbapenem-nonsusceptible Enterobacterales collected in US hospitals in a five-year period and activity of ceftazidime/avibactam and comparator agents.
The study identified several carbapenemase genes, including bla KPC, bla OXA-181, bla NDM-1, bla NDM-5, bla VIM-1, and bla IMP-27, in carbapenem-nonsusceptible Enterobacterales isolates. New KPC variants, such as bla KPC-58, were characterized and showed resistance to ceftazidime/avibactam and low meropenem MIC values.
Prevalence of carbapenemase genes among carbapenem-nonsusceptible Enterobacterales collected in US hospitals in a five-year period and activity of ceftazidime/avibactam and comparator agents.
The study identified several carbapenemase genes, including bla KPC, bla OXA-181, bla NDM-1, bla NDM-5, bla VIM-1, and bla IMP-27, in carbapenem-nonsusceptible Enterobacterales isolates. New KPC variants, such as bla KPC-58, were characterized and showed resistance to ceftazidime/avibactam and low meropenem MIC values.
Resistance and virulence features of hypermucoviscous Klebsiella pneumoniae from bloodstream infections: Results of a nationwide Italian surveillance study.
The study identified various AMR genes in hypermucoviscous Klebsiella pneumoniae isolates, including bla KPC-3, bla KPC-2, CMY-16, CTX-M-15, bla SHV-2, bla DHA-1, bla CTX-M-65, qnr, aadA1, aac(6')-Ib, sul1, dfrA1, mph(A), erm(B), floR, and fosA3. These genes conferred resistance to multiple antibiotics, including carbapenems, cephalosporins, fluoroquinolones, aminoglycosides, sulfonamides, trimethoprim, macrolides, and fosfomycin.
Resistance and virulence features of hypermucoviscous Klebsiella pneumoniae from bloodstream infections: Results of a nationwide Italian surveillance study.
The study identified various AMR genes in hypermucoviscous Klebsiella pneumoniae isolates, including bla KPC-3, bla KPC-2, CMY-16, CTX-M-15, bla SHV-2, bla DHA-1, bla CTX-M-65, qnr, aadA1, aac(6')-Ib, sul1, dfrA1, mph(A), erm(B), floR, and fosA3. These genes conferred resistance to multiple antibiotics, including carbapenems, cephalosporins, fluoroquinolones, aminoglycosides, sulfonamides, trimethoprim, macrolides, and fosfomycin.
Emergence of a Fatal ST11-KL64 Tigecycline-Resistant Hypervirulent Klebsiella pneumoniae Clone Cocarrying bla(NDM) and bla(KPC) in Plasmids.
The study identifies a tigecycline-resistant ST11-KL64 hypervirulent Klebsiella pneumoniae isolate that co-carries bla(KPC) and bla(NDM) genes, leading to multidrug resistance and a fatal blood infection.
In vitro activity of ceftazidime/avibactam against carbapenem-nonsusceptible Klebsiella penumoniae isolates collected during the first wave of the SARS-CoV-2 pandemic: a Southern Italy, multicenter, surveillance study.
The study identified three ceftazidime/avibactam-resistant (CAZ/AVI-R) isolates carrying different carbapenemases: KPC-31, VIM-1, and NDM-1. These isolates belonged to high-risk clones (ST101, ST45, and ST147) and showed varying levels of resistance to other antibiotics.
Epidemiological characteristics an outbreak of ST11 multidrug-resistant and hypervirulent Klebsiella pneumoniae in Anhui, China.
Evolution of Virulence, Fitness, and Carbapenem Resistance Transmission in ST23 Hypervirulent Klebsiella pneumoniae with the Capsular Polysaccharide Synthesis Gene wcaJ Inserted via Insertion Sequence Elements.
The study identifies the blaKPC-2 carbapenemase gene as a key factor in carbapenem resistance in ST23 hypervirulent Klebsiella pneumoniae isolates, with wcaJ gene inactivation linked to altered capsule synthesis and virulence.
Insights on the performance of phenotypic tests versus genotypic tests for the detection of carbapenemase-producing Gram-negative bacilli in resource-limited settings.
The study evaluated the performance of phenotypic tests (MHT, mCIM, BCT, CDT) versus genotypic tests for detecting carbapenemase-producing Gram-negative bacilli (CPO). It identified bla KPC, bla NDM, bla VIM, bla GIM, and bla OXA−48 as the most prevalent carbapenemase genes among the isolates.
Evolution of Beta-Lactamases in Urinary Klebsiella pneumoniae Isolates from Croatia; from Extended-Spectrum Beta-Lactamases to Carbapenemases and Colistin Resistance.
The study identified the evolution of beta-lactamases in urinary Klebsiella pneumoniae isolates from Croatia, highlighting the emergence of CTX-M-15, OXA-48, and KPC-2 beta-lactamases, along with the increasing resistance to carbapenems and colistin.
Molecular Mechanisms Mediating Ceftazidime/Avibactam Resistance Amongst Carbapenem-Resistant Klebsiella pneumoniae Isolates from Cancer Patients.
The study identified bla KPC, bla OXA-48, bla NDM-1, and bla IMP as the primary genes responsible for ceftazidime/avibactam resistance in carbapenem-resistant Klebsiella pneumoniae isolates from cancer patients. Additionally, the absence of ompK35 and ompK36 was associated with resistance.
A novel KPC-113 variant conferring carbapenem and ceftazidime-avibactam resistance in a multidrug-resistant Pseudomonas aeruginosa isolate.
A novel KPC-113 variant conferring carbapenem and ceftazidime-avibactam resistance in a multidrug-resistant Pseudomonas aeruginosa isolate.
Identification and characterization of bacteria isolated from patients with cystic fibrosis in Jordan.
The study identified blaKPC, blaNDM, blaVIM, and mecA as the primary resistance genes in bacteria isolated from patients with cystic fibrosis in Jordan. These genes conferred resistance to carbapenems and beta-lactams, highlighting the challenges in treating multidrug-resistant infections.
Klebsiella pneumoniae ST147: and then there were three carbapenemases.
The study reports the first complete genome sequence of a K. pneumoniae isolate harboring three distinct carbapenemases: blaKPC-2, blaNDM-1, and blaOXA-48. These genes were located on different plasmids and were capable of conjugative transfer to E. coli.
Bloodstream Infections in Patients with Rectal Colonization by Carbapenem-Resistant Enterobacteriaceae: A Prospective Cohort Study.
The study identified blaKPC-2 as a prevalent carbapenemase gene in CRE isolates, contributing to resistance against carbapenems.
Emergence of KPC-31, a KPC-3 Variant Associated with Ceftazidime-Avibactam Resistance, in an Extensively Drug-Resistant ST235 Pseudomonas aeruginosa Clinical Isolate.
The study describes the emergence of KPC-31, a variant of KPC-3, in a ceftazidime-avibactam-resistant Pseudomonas aeruginosa isolate, highlighting its association with resistance to ceftazidime-avibactam and ceftolozane-tazobactam.
Clonal Dissemination of Multidrug-Resistant and Hypervirulent Klebsiella pneumoniae Clonal Complex in a Chinese Hospital.
The study identifies multiple lineages of carbapenem-resistant hypervirulent Klebsiella pneumoniae (cr-hvKp) within the CC15 clonal complex, highlighting the dissemination of resistance genes such as bla KPC-2, bla SHV-28, and bla TEM-1B, along with virulence plasmids contributing to hypervirulence.
Emerging and re-emerging KPC-producing hypervirulent Pseudomonas aeruginosa ST697 and ST463 between 2010 and 2021.
The study identified 11 KPC-2-producing P. aeruginosa isolates, highlighting the emergence of hypervirulent ST697 and ST463 clones with multidrug resistance and high virulence.
Clinical Performance of the Osmotic Shock-MALDI MS Method to Detect Klebsiella pneumoniae Carbapenemase in Clinical Isolates.
The study evaluates the OS-MALDI MS method for detecting KPC carbapenemase in clinical isolates, demonstrating high sensitivity and specificity compared to other methods.
Coexistence of tet(A) and bla(KPC-2) in the ST11 hypervirulent tigecycline- and carbapenem-resistant Klebsiella pneumoniae isolated from a blood sample.
The study identifies the coexistence of the tet(A) gene and bla(KPC-2) in a hypervirulent tigecycline- and carbapenem-resistant ST11 Klebsiella pneumoniae strain, XJ-K2, isolated from a blood sample in China.
Hypervirulent carbapenem-resistant Klebsiella pneumoniae causing highly fatal meningitis in southeastern China.
The study identified hypervirulent carbapenem-resistant Klebsiella pneumoniae (Hv-CRKP) strains with high mortality rates, highlighting the presence of resistance genes such as blaKPC-2, blaCTX-M-65, and blaSHV, along with virulence genes like peg-344, iucA, iroB, rmpA, and rmpA2.
Evaluation of phenotypic detection of carbapenemase-producing Pseudomonas spp. from clinical isolates.
The study evaluated the performance of phenotypic tests (Carba NP, Blue Carba, and mCIM/eCIM) for detecting carbapenemase-producing Pseudomonas spp. in Brazil. It identified several carbapenemase genes, including bla VIM-2, bla SPM-1, bla IMP-10, bla VIM-24, bla NDM-1, and bla KPC-2, in clinical isolates.
Whole-Genome Sequence Analysis of Carbapenem-Heteroresistant Klebsiella pneumoniae and Escherichia coli Isolates.
The study identified blaKPC-2 and blaOXA-48 genes as responsible for carbapenem resistance in E. coli and K. pneumoniae isolates, respectively.
Genomic Analysis and Molecular Characteristics in Carbapenem-Resistant Klebsiella pneumoniae Strains.
The study identified several AMR genes, including bla KPC-2, bla SHV-11, bla TEM-1, CTX-M, qnrS, oqxB1, sul1, sul2, int1, iutA, and rmpA2, in carbapenem-resistant Klebsiella pneumoniae strains.
Whole-Genome Sequencing-Based Species Classification, Multilocus Sequence Typing, and Antimicrobial Resistance Mechanism Analysis of the Enterobacter cloacae Complex in Southern China.
The study identified 80 antibiotic resistance genes in 172 Enterobacter cloacae complex isolates, with a focus on beta-lactamases, aminoglycosides, and fluoroquinolones. Key genes included blaACT-2, blaACT-3, blaACT-6, blaACT-9, blaACT-12, blaTEM-1D, blaCTX-M-3, blaSHV-12, blaNDM-1, blaNDM-5, blaIMP-1, blaIMP-4, blaIMP-26, blaKPC-2, qnrS2, qnrE1, aac(6')-Ib, aac(6')-IIc, aph(3')-Ib, aph(6)-Id, aadA, sul2, dfrA12, tetA, ereA, floR, catA2, mcr-10, arr-6, and fosA3.
Genomic Characterization of an Extensively Drug-Resistant Extra-Intestinal Pathogenic (ExPEC) Escherichia coli Clinical Isolate Co-Producing Two Carbapenemases and a 16S rRNA Methylase.
The study describes an extensively drug-resistant (XDR) E. coli ST361 isolate co-carrying bla KPC-3, bla NDM-5, and various other resistance genes on multiple plasmids, showing resistance to nearly all antibiotics except tigecycline, colistin, and fosfomycin.
Dissemination of High-Risk Clones Enterobacterales among Bulgarian Fecal Carriage Isolates.
The study identified several AMR genes, including bla CTX-M-15, bla CTX-M-3, bla CTX-M-27, bla CTX-M-14, bla CTX-M-9, bla SHV-1, bla SHV-11, bla TEM-1, bla NDM-1, bla KPC-2, bla CMY-4, bla DHA-1, and bla CMY-2, which confer resistance to various beta-lactam antibiotics. High-risk clones such as ST11, ST258, and ST15 were found to be prevalent among Klebsiella pneumoniae isolates, while ST131 was the most common among E. coli isolates.
A simple label-free method reveals bacterial growth dynamics and antibiotic action in real-time.
The study identifies the blaKPC gene in Klebsiella pneumoniae 1705, which confers resistance to carbapenems. The gene's presence was determined by comparing growth dynamics between strains with and without the gene.
Identification of KPC-112 from an ST15 Klebsiella pneumoniae Strain Conferring Resistance to Ceftazidime-Avibactam.
The study identifies a novel KPC variant, KPC-112, which confers resistance to ceftazidime-avibactam. Additionally, various other resistance genes and mutations were characterized, contributing to multidrug resistance in the isolate.
Identification of KPC-112 from an ST15 Klebsiella pneumoniae Strain Conferring Resistance to Ceftazidime-Avibactam.
Identification of KPC-112 from an ST15 Klebsiella pneumoniae Strain Conferring Resistance to Ceftazidime-Avibactam.
Emergence of Extensively Drug-Resistant and Hypervirulent KL2-ST65 Klebsiella pneumoniae Harboring bla(KPC-3) in Beijing, China.
The study identifies the emergence of extensively drug-resistant (XDR) and hypervirulent KL2-ST65 Klebsiella pneumoniae in Beijing, China, harboring the blaKPC-3 gene, which confers resistance to carbapenems, cephalosporins, and penicillins. Additionally, the blaCTX-M-3 gene was detected, contributing to resistance against cephalosporins. Other resistance genes such as blaOXA-1, aac(6')-Ib-cr, and qnrS1 were also identified, providing resistance to penicillins, aminoglycosides, and quinolones, respectively.
Antimicrobial resistance: mechanisms and implications
The review discusses various molecular mechanisms of antibiotic resistance, including beta-lactamases, aminoglycoside-modifying enzymes, tetracycline resistance genes, and efflux pumps, highlighting their roles in conferring resistance to multiple antibiotics.
Subinhibitory antibiotic concentrations promote the horizontal transfer of plasmid-borne resistance genes from Klebsiellae pneumoniae to Escherichia coli.
Subinhibitory concentrations of meropenem, ciprofloxacin, cefotaxime, and amikacin significantly increased the conjugation frequency of the plasmid-borne resistance gene bla KPC from Klebsiella pneumoniae SW1780 to Escherichia coli J53.
Novel insights related to the rise of KPC-producing Enterobacter cloacae complex strains within the nosocomial niche.
The study identifies a novel conjugative IncM1 plasmid, pDCCK1-KPC, carrying the bla KPC-2 gene, which confers resistance to carbapenems and beta-lactams in KPC-producing Enterobacter hormaechei and Klebsiella pneumoniae strains.
Co-Existence of KPC-2, LAP-2, and CTX-M-65 in an ST1469 Multidrug-Resistant Klebsiella pneumoniae Strain in China.
The study identified a multidrug-resistant Klebsiella pneumoniae strain SJ25 co-harboring blaKPC-2, blaCTX-M-65, and blaLAP-2, highlighting the need for continued surveillance of β-lactamase-producing K. pneumoniae.
Clinical Risk Factors and Microbiological and Intestinal Characteristics of Carbapenemase-Producing Enterobacteriaceae Colonization and Subsequent Infection.
The study identifies bla OXA-48-like, bla KPC-2, and bla NDM-1 as the primary carbapenemase genes associated with CRE colonization and subsequent infection, highlighting their role in carbapenem resistance.
Competitive Transmission of Carbapenem-Resistant Klebsiella pneumoniae in a Newly Opened Intensive Care Unit.
The study identified multiple carbapenem-resistant Klebsiella pneumoniae (CRKP) clones, primarily ST11, carrying genes such as blaKPC-2, blaNDM-1, and blaNDM-5, contributing to carbapenem resistance. Some clones showed extensive environmental contamination and transmission dynamics.
Outbreak of Klebsiella pneumoniae ST11 Resistant To Ceftazidime-Avibactam Producing KPC-31 and the Novel Variant KPC-115 during COVID-19 Pandemic in Argentina.
The study describes an outbreak of Klebsiella pneumoniae ST11 producing KPC-31 and a novel variant KPC-115, which are resistant to ceftazidime-avibactam. The KPC-115 variant shows a 6-nucleotide deletion and a substitution compared to KPC-3.
Outbreak of Klebsiella pneumoniae ST11 Resistant To Ceftazidime-Avibactam Producing KPC-31 and the Novel Variant KPC-115 during COVID-19 Pandemic in Argentina.
The study describes an outbreak of Klebsiella pneumoniae ST11 producing KPC-31 and a novel variant KPC-115, which are resistant to ceftazidime-avibactam. The KPC-115 variant shows a 6-nucleotide deletion and a substitution compared to KPC-3.
Clonal Spread of Carbapenem-Resistant Klebsiella pneumoniae Sequence Type 11 in Chinese Pediatric Patients.
The study identifies bla KPC-2 and mcr-9 as key resistance genes in carbapenem-resistant Klebsiella pneumoniae ST11 strains, highlighting their role in the clonal spread of multidrug-resistant strains in a pediatric hospital setting.
Human microbiota drives hospital-associated antimicrobial resistance dissemination in the urban environment and mirrors patient case rates.
The study identifies the blaKPC-2 gene as a key factor in carbapenem resistance, highlighting the role of hospital-associated K. pneumoniae ST-11 clones in the dissemination of antimicrobial resistance in urban environments.
Emergence of Ceftazidime-Avibactam Resistance and Decreased Virulence in Carbapenem-Resistant ST11 Klebsiella pneumoniae During Antibiotics Treatment.
The study reports the emergence of CZA resistance and decreased virulence in ST11 CRKP during antibiotic treatment. The resistance was due to a mutation in the bla KPC-2 gene, resulting in bla KPC-33, which conferred resistance to several antibiotics. Additionally, a frameshift mutation in the rmpA gene led to reduced virulence.
Emergence of Ceftazidime-Avibactam Resistance and Decreased Virulence in Carbapenem-Resistant ST11 Klebsiella pneumoniae During Antibiotics Treatment.
The study reports the emergence of CZA resistance and decreased virulence in ST11 CRKP during antibiotic treatment. The resistance was due to a mutation in the bla KPC-2 gene, resulting in bla KPC-33, which conferred resistance to several antibiotics. Additionally, a frameshift mutation in the rmpA gene led to reduced virulence.
Coexistence of Multidrug Resistance and Virulence in a Single Conjugative Plasmid from a Hypervirulent Klebsiella pneumoniae Isolate of Sequence Type 25.
The study identifies a conjugative plasmid pCY814036-iucA carrying multidrug resistance genes and virulence factors, and another plasmid pCY814036-KPC2 harboring bla KPC-2 and rmtB, contributing to carbapenem resistance and hypervirulence in a Klebsiella pneumoniae isolate.
Molecular Epidemiology of Carbapenem-Resistant Klebsiella pneumoniae in a Tertiary Hospital in Northern China.
The study identified bla KPC-2, bla GES, bla NDM-1, and bla IMP as the main carbapenemase genes in CRKP isolates. Additionally, various ESBL genes, aminoglycoside resistance genes, and PMQR genes were detected.
Characterization of virulence and antimicrobial resistance genes of Aeromonas media strain SD/21-15 from marine sediments in comparison with other Aeromonas spp.
The study characterized multiple antimicrobial resistance (AMR) genes in Aeromonas media strain SD/21-15, including beta-lactamases (bla MOX-9, bla OXA-427), carbapenemases (crp), polymyxin resistance genes (mcr, mcr-3, mcr-3.6), tetracycline resistance (tet(E)), quinolone resistance (qnrS2), and multidrug efflux pumps (mexB).
Horizontal gene transfer via OMVs co-carrying virulence and antimicrobial-resistant genes is a novel way for the dissemination of carbapenem-resistant hypervirulent Klebsiella pneumoniae.
The study identifies several beta-lactamase genes (bla SHV-1, bla CTX-M-65, bla KPC-2, bla NDM-1, and bla CTX-M-30) in the CR-HvKP strain NUHL30457, demonstrating their presence in OMVs and their potential role in horizontal gene transfer.
Detecting Carbapenemases in Animal and Food Samples by Droplet Digital PCR.
The study demonstrates the effectiveness of droplet digital PCR (ddPCR) in detecting bla KPC, bla VIM, and bla OXA-48-like carbapenemase genes in various animal and food samples, highlighting the prevalence of bla OXA-48-like genes.
Genomic Analysis of Klebsiella pneumoniae ST258 Strain Coproducing KPC-2 and CTX-M-14 Isolated from Poultry in the Brazilian Amazon Region.
The study characterizes a multidrug-resistant Klebsiella pneumoniae ST258 strain (KP411) isolated from poultry in the Brazilian Amazon, which carries several AMR genes including blaKPC-2, blaCTX-M-14, and others, highlighting the potential of food-producing animals as reservoirs of MDR clones.
Efficacy of Vaporized Hydrogen Peroxide Combined with Silver Ions against Multidrug-Resistant Gram-Negative and Gram-Positive Clinical Isolates.
The study evaluated the efficacy of 8% vaporized hydrogen peroxide combined with 30 mg/L silver ions against multidrug-resistant (MDR) clinical isolates of Klebsiella pneumoniae, Pseudomonas aeruginosa, and methicillin-resistant Staphylococcus aureus. It identified specific carbapenemase genes (bla KPC, bla VIM, bla IMP, bla NDM, and bla OXA-48) associated with resistance in these isolates.
Molecular Characterization of Carbapenem-Resistant Acinetobacter baumannii with Special Reference to Carbapenemases: A Systematic Review.
The paper reviews the molecular characteristics of carbapenem-resistant Acinetobacter baumannii, focusing on carbapenemases. It identifies several beta-lactamase genes, including OXA-23, OXA-51, OXA-58, VIM, NDM, IMP, KPC, and GES, which confer resistance to carbapenems. The study highlights the role of mobile genetic elements in the dissemination of these resistance genes.
Molecular Characterization of Carbapenem-Resistant Acinetobacter baumannii with Special Reference to Carbapenemases: A Systematic Review.
The paper reviews the molecular characteristics of carbapenem-resistant Acinetobacter baumannii, focusing on carbapenemases. It identifies several beta-lactamase genes, including OXA-23, OXA-51, OXA-58, VIM, NDM, IMP, KPC, and GES, which confer resistance to carbapenems. The study highlights the role of mobile genetic elements in the dissemination of these resistance genes.
Molecular Characterization of Carbapenem-Resistant Acinetobacter baumannii with Special Reference to Carbapenemases: A Systematic Review.
The paper reviews the molecular characteristics of carbapenem-resistant Acinetobacter baumannii, focusing on carbapenemases. It identifies several beta-lactamase genes, including OXA-23, OXA-51, OXA-58, VIM, NDM, IMP, KPC, and GES, which confer resistance to carbapenems. The study highlights the role of mobile genetic elements in the dissemination of these resistance genes.
Molecular Characterization of Carbapenem-Resistant Acinetobacter baumannii with Special Reference to Carbapenemases: A Systematic Review.
The paper reviews the molecular characteristics of carbapenem-resistant Acinetobacter baumannii, focusing on carbapenemases. It identifies several beta-lactamase genes, including OXA-23, OXA-51, OXA-58, VIM, NDM, IMP, KPC, and GES, which confer resistance to carbapenems. The study highlights the role of mobile genetic elements in the dissemination of these resistance genes.
Genetic and Phenotypic Characterization of Multidrug-Resistant Klebsiella pneumoniae from Liver Abscess.
Genetic and Phenotypic Characterization of Multidrug-Resistant Klebsiella pneumoniae from Liver Abscess.
Boronic Acid Transition State Inhibitors as Potent Inactivators of KPC and CTX-M beta-lactamases: Biochemical and Structural Analyses.
The study characterizes the inhibitory effects of BATSIs, S02030 and MB_076, against KPC-2 and CTX-M-96 beta-lactamases, demonstrating their potent activity as beta-lactamase inhibitors.
Carbapenem-resistant hypermucoviscous Klebsiella pneumoniae clinical isolates from a tertiary hospital in China: Antimicrobial susceptibility, resistance phenotype, epidemiological characteristics, microbial virulence, and risk factors.
The study identified bla KPC-2, bla NDM, bla IMP, and bla OXA-48-like as the main carbapenemase genes in carbapenem-resistant K. pneumoniae (CRKP) isolates. These genes conferred resistance to carbapenems, cephalosporins, and fluoroquinolones. The study also highlighted the importance of CZA in treating CRKP infections.
The application of targeted nanopore sequencing for the identification of pathogens and resistance genes in lower respiratory tract infections.
The study utilized targeted nanopore sequencing (TNPseq) to identify pathogens and antimicrobial resistance (AMR) genes in lower respiratory tract infections (LRTIs). TNPseq demonstrated higher sensitivity than conventional culture methods in detecting pathogens and AMR genes, with blaTEM being the most prevalent AMR gene identified.
Characterization of a Conjugative Hybrid Plasmid Coharboring bla(KPC-2) and bla(IMP-4) in a Klebsiella quasipneumoniae Clinical Isolate.
The study characterizes a conjugative hybrid plasmid co-harboring bla(KPC-2) and bla(IMP-4) in a clinical isolate of Klebsiella quasipneumoniae, highlighting the evolution and transmission of resistance genes through recombination events.
Expanded catalogue of metagenome-assembled genomes reveals resistome characteristics and athletic performance-associated microbes in horse.
The study identified a diverse array of antibiotic resistance genes (ARG) in the horse gut microbiome, highlighting the widespread use of antibiotics in horse management. The resistome characteristics were analyzed alongside the identification of microbes associated with athletic performance.
Molecular and Clinical Characteristics of Carbapenem-Resistant Klebsiella pneumoniae Isolates at a Tertiary Hospital in Wuhan, China.
The study identified multiple carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates carrying various resistance genes, including blaKPC-1, blaCTX-M-65, blaTEM-1, blaSHV-182, rmtB, aadA2, APH(3')-Ia, sul1, sul2, QnrS1, mphA, FosA6, floR, and tet(A). These genes confer resistance to carbapenems, beta-lactams, aminoglycosides, sulfonamides, fluoroquinolones, macrolides, fosfomycin, chloramphenicol, and tetracyclines.
An XDR Pseudomonas aeruginosa ST463 Strain with an IncP-2 Plasmid Containing a Novel Transposon Tn6485f Encoding bla(IMP-45) and bla(AFM-1) and a Second Plasmid with Two Copies of bla(KPC-2).
The study identifies a novel transposon Tn6485f containing blaIMP-45 and blaAFM-1, along with a second plasmid with two copies of blaKPC-2, contributing to the XDR phenotype of the P. aeruginosa ST463 strain PA30.
Genomic Characterization of KPC-31 and OXA-181 Klebsiella pneumoniae Resistant to New Generation of β-Lactam/β-Lactamase Inhibitor Combinations.
The study reports the genomic characterization of a multidrug-resistant Klebsiella pneumoniae strain co-harboring blaKPC-31 and blaOXA-181, along with porin modifications (ompK35 truncation and ompK36GD mutation), leading to resistance against carbapenems and other antibiotic classes, including CZA, M/V, and I/R.
Prevalence of Carbapenemase and Extended-Spectrum β-Lactamase Producing Enterobacteriaceae: A Cross-Sectional Study.
The study identified the prevalence of carbapenemase (KPC, NDM, OXA-48) and extended-spectrum beta-lactamase (ESBL) producing Enterobacteriaceae, highlighting the high levels of resistance to carbapenems and third-generation cephalosporins.
A Cross-Validated Feature Selection (CVFS) approach for extracting the most parsimonious feature sets and discovering potential antimicrobial resistance (AMR) biomarkers.
The study presents a Cross-Validated Feature Selection (CVFS) approach for identifying the most parsimonious gene sets for predicting antimicrobial resistance (AMR) from bacterial pan-genomes. The CVFS approach was able to extract both known and novel AMR genes, demonstrating its effectiveness in selecting relevant features for AMR prediction.
Evaluation of in-vitro susceptibility of ß-lactam-resistant Gram-negative bacilli to ceftazidime-avibactam and ceftolozane-tazobactam from clinical samples of a general hospital in southern Brazil.
The study identified bla CTX-M, bla SHV, bla KPC, bla NDM-1, and bla VIM as the primary β-lactamase genes responsible for resistance to ceftazidime-avibactam and ceftolozane-tazobactam in β-lactam-resistant Gram-negative bacilli.
Risk factors and molecular epidemiology of fecal carriage of carbapenem resistant Enterobacteriaceae in patients with liver disease.
The study identified bla KPC-2 and bla NDM as the primary carbapenemase genes in CRE strains, with bla KPC-2 being the most prevalent. Additionally, the polymyxin resistance gene mcr-1 was detected in some E. coli strains.
Development of a novel loop-mediated isothermal amplification assay for ß-lactamase gene identification using clinical isolates of Gram-negative bacteria.
The study developed LAMP assays for four β-lactamase genes (bla KPC, bla NDM-1, bla IMP-1 group, and bla VIM) and validated their specificity and sensitivity using clinical isolates of Gram-negative bacteria.
Emergence and Inter- and Intrahost Evolution of Pandrug-Resistant Klebsiella pneumoniae Coharboring tmexCD1-toprJ1, bla (NDM-1), and bla (KPC-2).
The study identifies the emergence of pandrug-resistant Klebsiella pneumoniae strains coharboring tmexCD1-toprJ1, bla(NDM-1), and bla(KPC-2). These genes contribute to resistance against tigecycline, carbapenems, and other antibiotics.
Rapid culture-independent loop-mediated isothermal amplification detection of antimicrobial resistance markers from environmental water samples.
The study developed and validated LAMP assays for rapid detection of mcr-1, blaKPC, blaOXA-48, blaOXA-23, and blaVIM genes in environmental water samples, demonstrating high sensitivity and specificity.
Ceftazidime-avibactam resistance in Klebsiella pneumoniae sequence type 37: a decade of persistence and concealed evolution.
The study identifies three KPC variants (KPC-31, KPC-70, and KPC-110) in ceftazidime-avibactam-resistant Klebsiella pneumoniae ST37 isolates, demonstrating their role in CZA resistance through experimental validation.
Ceftazidime-avibactam resistance in Klebsiella pneumoniae sequence type 37: a decade of persistence and concealed evolution.
The study identifies three KPC variants (KPC-31, KPC-70, and KPC-110) in ceftazidime-avibactam-resistant Klebsiella pneumoniae ST37 isolates, demonstrating their role in CZA resistance through experimental validation.
Ceftazidime-avibactam resistance in Klebsiella pneumoniae sequence type 37: a decade of persistence and concealed evolution.
The study identifies three KPC variants (KPC-31, KPC-70, and KPC-110) in ceftazidime-avibactam-resistant Klebsiella pneumoniae ST37 isolates, demonstrating their role in CZA resistance through experimental validation.
Evaluation of Xpert Carba-R for detecting carbapenemase-producing organisms in South Africa.
The Xpert Carba-R assay demonstrated high performance in detecting carbapenemase-producing organisms, with 98% sensitivity and 97% specificity. It successfully identified various carbapenemase genes, including bla KPC, bla NDM, bla VIM, bla OXA-48, and bla IMP-1, in both cultured isolates and spiked rectal swabs.
Trends of β-Lactamase Occurrence Among Escherichia coli and Klebsiella pneumoniae in United States Hospitals During a 5-Year Period and Activity of Antimicrobial Agents Against Isolates Stratified by β-Lactamase Type.
The study identified various β-lactamase genes, including bla CTX-M-15, bla CTX-M-55, bla CTX-M-14, bla CTX-M-27, bla SHV-12, bla SHV-7, bla SHV-27, bla TEM-1, bla TEM-12, bla CMY-2, bla DHA-1, bla FOX-5, bla OXA-1, bla OXA-1-like, bla KPC-2, bla KPC-3, bla NDM-1, bla NDM-5, bla OXA-181, bla OXA-48, and bla OXA-232, which confer resistance to β-lactam antibiotics. The study also found that CTX-M-15 was the most prevalent β-lactamase gene, and there was a notable increase in ESBL-producing isolates among K. pneumoniae.
Trends of β-Lactamase Occurrence Among Escherichia coli and Klebsiella pneumoniae in United States Hospitals During a 5-Year Period and Activity of Antimicrobial Agents Against Isolates Stratified by β-Lactamase Type.
The study identified various β-lactamase genes, including bla CTX-M-15, bla CTX-M-55, bla CTX-M-14, bla CTX-M-27, bla SHV-12, bla SHV-7, bla SHV-27, bla TEM-1, bla TEM-12, bla CMY-2, bla DHA-1, bla FOX-5, bla OXA-1, bla OXA-1-like, bla KPC-2, bla KPC-3, bla NDM-1, bla NDM-5, bla OXA-181, bla OXA-48, and bla OXA-232, which confer resistance to β-lactam antibiotics. The study also found that CTX-M-15 was the most prevalent β-lactamase gene, and there was a notable increase in ESBL-producing isolates among K. pneumoniae.
The Effect of β-Lactam Antibiotics on the Evolution of Ceftazidime/Avibactam and Cefiderocol Resistance in KPC-Producing Klebsiella pneumoniae.
The study identifies bla KPC-2 and bla KPC-33 as key genes contributing to resistance against ceftazidime-avibactam and cefiderocol in KPC-producing Klebsiella pneumoniae. Mutations in bla KPC-2, such as G532T (D179Y), 820-825dup (E275-A276dup), G532A (D179N), 721-726del (G242-T243del), and 802-816dup (K269-H273dup), enhance resistance levels.
The Effect of β-Lactam Antibiotics on the Evolution of Ceftazidime/Avibactam and Cefiderocol Resistance in KPC-Producing Klebsiella pneumoniae.
The study identifies bla KPC-2 and bla KPC-33 as key genes contributing to resistance against ceftazidime-avibactam and cefiderocol in KPC-producing Klebsiella pneumoniae. Mutations in bla KPC-2, such as G532T (D179Y), 820-825dup (E275-A276dup), G532A (D179N), 721-726del (G242-T243del), and 802-816dup (K269-H273dup), enhance resistance levels.
Epidemiology and Genetic Characteristics of Carbapenem-Resistant Escherichia coli in Chinese Intensive Care Unit Analyzed by Whole-Genome Sequencing: a Prospective Observational Study.
The study identified bla KPC-2 and bla NDM-5 as the main carbapenem resistance genes in carbapenem-resistant Escherichia coli (CREC) isolates. Additionally, various other resistance genes were detected, including extended-spectrum beta-lactamases (CTX-M-14, CTX-M-15, CTX-M-27, CTX-M-3, OXA-1, OXA-10, TEM-1), aminoglycoside resistance genes (aac(3)-IId, aac(3)-Iva, aac(6′)-Ib-AKT, aac(6′)-Ib-D181Y, aadA1, aadA2, aadA5, aph(3″)-Ib, aph(3′)-Ia, aph(4)-Ia, aph(6)-Id), quinolone resistance genes (qnrS1, qnrS2), tetracycline resistance genes (oqxA10, oqxB17), fosfomycin resistance gene (fosA3), and chloramphenicol resistance genes (cmlA1, cmlA5).
Frequency and diversity of carbapenemase-producing Enterobacterales recovered from untreated wastewater impacted by selective media containing cefotaxime and meropenem in Ohio, USA.
The study identified bla KPC and bla NDM genes as the primary carbapenemase resistance mechanisms in Enterobacterales isolated from untreated wastewater in Ohio, USA.
Isolation, characterization, therapeutic potency, and genomic analysis of a novel bacteriophage vB_KshKPC-M against carbapenemase-producing Klebsiella pneumoniae strains (CRKP) isolated from Ventilator-associated pneumoniae (VAP) infection of COVID-19 patients.
The study identifies the presence of carbapenemase genes blaKPC, blaNDM, and blaOXA-23 in carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates from COVID-19 patients. A novel bacteriophage vB_KshKPC-M was isolated and characterized, showing effective lytic activity against CRKP strains.
Prevalence and Characterization of Beta-Lactam and Carbapenem-Resistant Bacteria Isolated from Organic Fresh Produce Retailed in Eastern Spain.
The study identified various beta-lactamase genes, including bla_VIM, bla_IMP, bla_TEM, bla_OXA-48, bla_SHV, bla_CMY-2, and bla_KPC, in beta-lactam and carbapenem-resistant bacteria isolated from organic fresh produce in Valencia, Spain.
Ertapenem Supplemented Selective Media as a New Strategy to Distinguish β-Lactam-Resistant Enterobacterales: Application to Clinical and Wastewater Samples.
The study identifies blaIMP-4 and blaKPC as the primary carbapenemase genes responsible for carbapenem resistance in Enterobacterales, demonstrating their presence in clinical and environmental samples through experimental validation.
Nanoparticle-Based Plasmonic Biosensor for the Unamplified Genomic Detection of Carbapenem-Resistant Bacteria.
The study presents a nanoparticle-based plasmonic biosensor for the detection of carbapenem-resistant bacteria, specifically targeting the blaKPC gene encoding a carbapenemase. The biosensor successfully detects KPC-producing bacteria with high specificity and sensitivity.
The Impact of Harsh Stratospheric Conditions on Survival and Antibiotic Resistance Profile of Non-Spore Forming Multidrug Resistant Human Pathogenic Bacteria Causing Hospital-Associated Infections.
The study examined the impact of stratospheric conditions on the survival and antibiotic resistance of multidrug-resistant bacteria. It found that exposure to stratospheric conditions led to a decrease in antibiotic resistance, with no loss of resistance genes detected.
Genomic characterization of two carbapenem-resistant Serratia marcescens isolates causing bacteremia: Emergence of KPC-2-encoding IncR plasmids.
Two carbapenem-resistant Serratia marcescens isolates were found to carry blaKPC-2 on IncR plasmids, along with additional resistance genes such as qnrS1, aac6'-Ic, and blaCTX-M-14.
Emergence of Carbapenemase-Producing Hypervirulent Klebsiella pneumoniae in Switzerland.
The study identifies carbapenemase-producing hypervirulent Klebsiella pneumoniae isolates in Switzerland, including those with bla OXA-48, bla KPC-3, bla KPC-41, and bla NDM-1 genes, as well as virulence factors such as aerobactin, yersiniabactin, colibactin, and ferric iron uptake systems.
Emergence of Carbapenemase-Producing Hypervirulent Klebsiella pneumoniae in Switzerland.
The study identifies carbapenemase-producing hypervirulent Klebsiella pneumoniae isolates in Switzerland, including those with bla OXA-48, bla KPC-3, bla KPC-41, and bla NDM-1 genes, as well as virulence factors such as aerobactin, yersiniabactin, colibactin, and ferric iron uptake systems.
Molecular Mechanisms of Resistance to Ceftazidime/Avibactam in Clinical Isolates of Enterobacterales and Pseudomonas aeruginosa in Latin American Hospitals.
The study identifies multiple AMR genes and mutations contributing to CZA resistance in Enterobacterales and P. aeruginosa, including MBLs, blaKPC, blaVIM, blaIMP, blaNDM, blaSPM-1, and various mutations in genes related to efflux pumps, porins, and beta-lactamases.
Clonal transmission of polymyxin B-resistant hypervirulent Klebsiella pneumoniae isolates coharboring bla(NDM-1) and bla(KPC-2) in a tertiary hospital in China.
The study identifies blaKPC-2 and blaNDM-1 as carbapenem resistance genes in polymyxin B-resistant hypervirulent Klebsiella pneumoniae isolates. Mutations in phoQ (D150G), pmrB (R256G, T157P, S85R), and insertion mutations in mgrB contribute to polymyxin B resistance.
Carbapenem-Resistant and ESBL-Producing Enterobacterales Emerging in Central Texas.
The study identifies various carbapenemase genes (blaKPC, blaNDM, blaOXA-48) and the CTX-M-15 beta-lactamase, as well as mutations in gyrA and ompK36 contributing to resistance in Enterobacterales isolates from Central Texas.
Risk factors for infections caused by carbapenem-resistant Enterobacterales: an international matched case-control-control study (EURECA).
The study identified carbapenemase genes such as OXA-48, KPC, and NDM as significant contributors to carbapenem resistance in Enterobacterales, highlighting their role in CRE infections.
Prevalence of silver resistance determinants and extended-spectrum β-lactamases in bacterial species causing wound infection: First report from Bangladesh.
The study reports the first detection of silver resistance genes (silE, silS, silP) and extended-spectrum β-lactamases (CTX-M-1, NDM-1, KPC, OXA-48, VIM-1) in bacterial isolates from wound infections in Bangladesh. These genes were predominantly found in Enterobacterales and were associated with high levels of antimicrobial resistance.
Association between Intestinal Colonization and Extraintestinal Infection with Carbapenem-Resistant Klebsiella pneumoniae in Children.
The study identifies carbapenem-resistant Klebsiella pneumoniae (CRKP) strains with specific resistance genes such as blaKPC-2, blaNDM-1, and blaIMP-4, along with virulence factors like mrk, kfu, iuc, and iro. It also characterizes a novel subclone CR-hvKP KL19-ST15 associated with hypervirulence and poor patient outcomes.
Phenotypic and Genomic Characterization of ST133 Siderophore-Encoding Extensively Drug-Resistant Enterobacter hormaechei.
The study reports an ST133 extensively drug-resistant and virulent Enterobacter hormaechei strain, C210017, carrying mcr-9.1, blaKPC-2, and siderophore-encoding genes. The strain was found to be resistant to multiple antibiotics, including carbapenems and colistin, and showed high virulence in a Galleria mellonella infection model.
Integrative omics identifies conserved and pathogen-specific responses of sepsis-causing bacteria.
The study identified various AMR genes in sepsis-causing bacteria, including beta-lactamases, aminoglycoside-modifying enzymes, tetracycline resistance genes, and others, highlighting the complexity of AMR in these pathogens.
Ceftazidime-Avibactam plus aztreonam synergistic combination tested against carbapenem-resistant Enterobacterales characterized phenotypically and genotypically: a glimmer of hope.
The study identified several carbapenemase genes including bla NDM, bla VIM, bla OXA-48, and bla KPC in carbapenem-resistant Enterobacterales. The combination of ceftazidime-avibactam and aztreonam showed a synergistic effect against these resistant isolates.
Cooperative antibiotic resistance facilitates horizontal gene transfer.
The study demonstrates that plasmid-encoded KPC-2 beta-lactamase enhances cooperative antibiotic resistance, leading to increased rescue of non-producers and efficient plasmid transfer.
Nanopore-based enrichment of antimicrobial resistance genes - a case-based study.
The study identifies NDM, KPC, and VIM carbapenemases in Raoultella ornithinolytica using PCR and nanopore sequencing, highlighting their role in carbapenem resistance.
High prevalence of extensively drug resistant and extended spectrum beta lactamases (ESBLs) producing uropathogenic Escherichia coli isolated from Faisalabad, Pakistan.
The study identified several ESBL genes including TEM-1, CTX-M1, CTX-M15, OXA-1, KPC, VIM, and GES in uropathogenic E. coli isolates from Faisalabad, Pakistan. These genes were associated with resistance to various beta-lactam antibiotics, highlighting the high prevalence of multidrug-resistant strains.
Tautomer-Specific Deacylation and Ω-Loop Flexibility Explain the Carbapenem-Hydrolyzing Broad-Spectrum Activity of the KPC-2 beta-lactamase.
The study characterizes KPC-2, a carbapenemase that hydrolyzes various β-lactam antibiotics, revealing the structural and dynamic basis for its broad-spectrum activity.
One Health Surveillance of Antimicrobial Use and Resistance: Challenges and Successes of Implementing Surveillance Programs in Sri Lanka.
The study identified CTX-M-15, CTX-M-14, and OXA-48 beta-lactamase genes in E. coli isolates from human, poultry, and aquaculture sectors in Sri Lanka, indicating the presence of extended-spectrum beta-lactamase (ESBL) resistance.
Characterization of Third Generation Cephalosporin- and Carbapenem-Resistant Aeromonas Isolates from Municipal and Hospital Wastewater.
The study identified several carbapenemase and ESBL genes in Aeromonas isolates from wastewater, highlighting the presence of bla KPC-2, bla VIM-2, bla OXA-48, bla IMP-13, bla GES-5, and bla MOX as significant contributors to antibiotic resistance.
Direct Detection of KPC Peak from Positive Blood Cultures Using MALDI-TOF MS: Are We There Yet?
The study demonstrates the feasibility of detecting KPC carbapenemase directly from positive blood cultures using MALDI-TOF MS, achieving high sensitivity and specificity.
Antibiotics and Bacterial Resistance-A Short Story of an Endless Arms Race.
The paper discusses the mechanisms of antibiotic actions and bacterial resistance strategies, highlighting the challenges posed by multidrug-resistant microbes and the need for new antimicrobial agents.
In vivo adaptive antimicrobial resistance in Klebsiella pneumoniae during antibiotic therapy.
The study identifies several AMR genes and mutations in Klebsiella pneumoniae that contribute to resistance against carbapenems, ceftazidime/avibactam, tigecycline, and colistin. Key findings include the acquisition of blaKPC and blaNDM harboring plasmids, specific mutations in blaKPC, porin deficiencies (ompK35 and ompK36), overexpression of efflux pumps (acrAB, oqxAB), and mutations in rpsJ, ramR, tetA, mgrB, and pmrB.
First report of coexistence of bla (KPC-2)-, bla (NDM-1)- and mcr-9-carrying plasmids in a clinical carbapenem-resistant Enterobacter hormaechei isolate.
The study reports the first case of a clinical Enterobacter hormaechei isolate carrying three distinct multidrug-resistant plasmids: pE1532-KPC (bla KPC-2), pE1532-NDM (bla NDM-1), and pE1532-MCR (mcr-9). These plasmids confer resistance to a wide range of antibiotics, highlighting the need for monitoring the spread of such resistance mechanisms.
Antimicrobial-Resistant Enterobacterales Recovered from the Environment of Two Zoological Institutions Include Enterobacter cloacae Complex ST171 Producing KPC-4 Carbapenemase.
The study identified Enterobacter cloacae complex ST171 isolates producing KPC-4 carbapenemase in the environment of zoological institutions, highlighting the presence of multidrug-resistant bacteria and the potential for AMR gene spread.
Outbreak report of polymyxin-carbapenem-resistant Klebsiella pneumoniae causing untreatable infections evidenced by synergy tests and bacterial genomes.
The study identifies multiple carbapenemase genes (blaKPC, blaNDM, blaOXA-48-like) and the mcr-1 gene, which confer resistance to carbapenems and polymyxin, respectively. Mutations in maeB and exuT are associated with resistance, survival, or proliferation in K. pneumoniae strains.
Epidemiological and Genetic Characteristics of Clinical Carbapenem-Resistant Pseudomonas aeruginosa Strains in Guangdong Province, China.
The study identified blaIMP-45 as a major determinant of meropenem resistance in P. aeruginosa, and mutations in oprD, mexR, nalD, and armR were associated with meropenem resistance.
Genotyping and molecular investigation of plasmid-mediated carbapenem resistant clinical Klebsiella pneumoniae isolates in Egypt.
The study identified the prevalence of carbapenem resistance genes (blaIMP, blaVIM, blaOXA-48, blaNDM, and blaKPC) in clinical Klebsiella pneumoniae isolates in Egypt, highlighting the high levels of resistance and the potential for horizontal gene transfer.
Genomic and functional characterization of carbapenem-resistant Klebsiella pneumoniae from hospital wastewater.
The study identified the bla KPC-2 gene as a major contributor to carbapenem resistance in Klebsiella pneumoniae isolates from hospital wastewater, demonstrating its successful in vitro transfer through conjugation.
Phylogenomics of Globally Spread Clonal Groups 14 and 15 of Klebsiella pneumoniae.
The study characterizes various AMR genes and mutations in K. pneumoniae clonal groups 14 and 15, highlighting the prevalence of bla CTX-M-15, bla OXA-232, bla NDM-1, and other beta-lactamases, along with quinolone resistance mechanisms.
Phylogenomics of Globally Spread Clonal Groups 14 and 15 of Klebsiella pneumoniae.
The study characterizes various AMR genes and mutations in K. pneumoniae clonal groups 14 and 15, highlighting the prevalence of bla CTX-M-15, bla OXA-232, bla NDM-1, and other beta-lactamases, along with quinolone resistance mechanisms.
Antibiotic Resistance Profiles and ARG Detection from Isolated Bacteria in a Culture-Dependent Study at the Codfish Industry Level.
The study identified various antibiotic resistance genes in bacteria isolated from codfish products and environmental samples, highlighting the presence of tetracycline, clindamycin, and oxacillin resistance genes in Staphylococcus spp., as well as beta-lactam and carbapenem resistance genes in Gram-negative bacteria.
Emergence and clonal dissemination of KPC-3-producing Pseudomonas aeruginosa in China with an IncP-2 megaplasmid.
The study identifies the first occurrence of KPC-3-producing Pseudomonas aeruginosa in China, highlighting the emergence of a multidrug-resistant IncP-2 megaplasmid carrying blaKPC-3.
Carbapenemase investigation with rapid phenotypic test (RESIST-4 O.K.N.V) and comparison with PCR in carbapenem-resistant Enterobacterales strains.
The study evaluated the RESIST-4 O.K.N.V test for detecting carbapenemases and found that it had high sensitivity and specificity for OXA-48, KPC, and VIM, but lower sensitivity for NDM. The most prevalent carbapenemase was OXA-48, followed by NDM, KPC, and VIM.
Whole-genome sequencing reveals high-risk clones of Pseudomonas aeruginosa in Guangdong, China.
The study identified multiple AMR genes, including aac(6')-IIa, aac(6')-Ib4, aac(6')-Ib7, aac(6')-Ib9, aac(6')-Ib10, aac(3)-IId, aadA, aadA2, aadA3, aadA7, aadA13, bla OXA-50-Like, bla OXA-1-Like, bla OXA-10-Like, bla OXA-21-Like, bla VIM-2, bla IMP-9, bla IMP-45, bla KPC-2, bla CTX-M-13, bla CARB-1, bla CARB-3, qnrVC1, qnrVC6, bla PDC-3, and bla PDC-5, in Pseudomonas aeruginosa isolates from Guangdong, China, highlighting the high prevalence of multidrug-resistant and carbapenem-resistant strains.
Phenotypic and Genetic Analysis of KPC-49, a KPC-2 Variant Conferring Resistance to Ceftazidime-Avibactam and Maintaining Resistance to Imipenem and Meropenem.
The study identifies KPC-49, a novel KPC-2 variant that confers resistance to ceftazidime-avibactam and maintains resistance to imipenem and meropenem. The variant exhibits a single nucleotide change (C487A) leading to an R163S amino acid substitution.
High-resolution characterization of short-term temporal variability in the taxonomic and resistome composition of wastewater influent.
The study highlights the importance of 24 h composite sampling over single grab sampling for capturing the full diversity of AMR gene families in wastewater influent, particularly for carbapenemase genes like bla VIM, bla KPC, bla IMP, and bla NDM, which were intermittently missed by grab sampling but reliably detected in composites.
International Epidemiology of Carbapenemase-Producing Escherichia coli.
The study identifies various carbapenemase genes, including bla NDM-5, bla KPC-2, and others, in carbapenemase-producing Escherichia coli isolates from different geographical regions.
Molecular Characterization of the Convergent Carbapenem-Resistant and Hypervirulent Klebsiella pneumoniae Strain K1-ST23, Collected in Chile during the COVID-19 Pandemic.
The study identified carbapenem-resistant hypervirulent Klebsiella pneumoniae strain K-2157, which harbors multiple AMR genes including blaSHV-190, blaTEM-1, blaOXA-9, blaKPC-2, fosA, oqxA, and oqxB, along with virulence-associated plasmids and an integrative and conjugative element (ICE).
Epidemiological and Antimicrobial Resistant Patterns, and Molecular Mechanisms of Carbapenem-Resistant Klebsiella pneumoniae Infections in ICU Patients.
The study identified bla KPC, bla OXA-23, and bla NDM as the top carbapenemase-encoding genes in CRKP strains. The virulence-associated gene irp 2 was dominant in the regional CRKP population, and nearly half of the CRKP isolates had the capsular polysaccharide serotype K14.K64 (wzi-64).
Comparison of In Vitro Activity of Ceftazidime-Avibactam and Imipenem-Relebactam against Clinical Isolates of Pseudomonas aeruginosa.
The study identified blaKPC-2 as a gene conferring resistance to ceftazidime and imipenem, and oprD mutations as a mechanism of imipenem resistance in Pseudomonas aeruginosa. Overexpression of ampC was also linked to resistance mechanisms.
Development and evaluation of a centrifugal disk system for the rapid detection of multiple pathogens and their antibiotic resistance genes in urinary tract infection.
The study developed a centrifugal disk system (LCD) for the rapid detection of multiple pathogens and their antibiotic resistance genes (ARGs) in urinary tract infections (UTIs). The LCD platform demonstrated high specificity and sensitivity for detecting pathogens and ARGs, including beta-lactamases (SHV, TEM, CTX-M-1 group, CTX-M-9 group), carbapenemases (KPC, NDM, IMP, OXA-23, OXA-24, OXA-48), ampC beta-lactamases (DHA, CMY), glycopeptide resistance (VanA), and methicillin resistance (mecA).
Genomic surveillance of multidrug-resistant Klebsiella in Wales reveals persistent spread of Klebsiella pneumoniae ST307 and adaptive evolution of pOXA-48-like plasmids.
The study identifies various carbapenemase genes, including bla_OXA-244, bla_KPC-2, bla_OXA-48, bla_VIM-4, bla_IMP-4, and bla_CTX-M-15, which contribute to multidrug resistance in Klebsiella isolates in Wales.
Multicenter Evaluation of the BIOFIRE Blood Culture Identification 2 Panel for Detection of Bacteria, Yeasts, and Antimicrobial Resistance Genes in Positive Blood Culture Samples.
The BIOFIRE BCID2 Panel demonstrated high sensitivity and specificity for detecting bacteria, yeasts, and antimicrobial resistance genes in positive blood culture samples. It effectively identified various AMR genes such as CTX-M, IMP, KPC, NDM, OXA-48-like, VIM, mecA/C, mcr-1, and vanA/B in Enterobacterales and other pathogens.
Characterization of bla(KPC-2) and bla(NDM-1) Plasmids of a K. pneumoniae ST11 Outbreak Clone.
The study characterizes bla(KPC-2) and bla(NDM-1) plasmids in a K. pneumoniae ST11 outbreak clone, highlighting their role in carbapenem resistance and conjugation capabilities.
Surveillance of carbapenem-resistant organisms using next-generation sequencing.
The study highlights the importance of next-generation sequencing (NGS) in detecting carbapenem-resistant organisms (CRO) and characterizing resistance genes such as blaKPC, blaNDM, blaVIM, blaIMP, and blaOXA-48. These genes were identified through PCR testing and sequencing, demonstrating their role in carbapenem resistance in Enterobacterales, Pseudomonas aeruginosa, and Acinetobacter baumannii.
Emergence of Aeromonas veronii strain co-harboring bla(KPC-2), mcr-3.17, and tmexC3.2-tmexD3.3-toprJ1b cluster from hospital sewage in China.
The study identifies a novel Aeromonas veronii strain co-harboring bla(KPC-2), mcr-3.17, and tmexC3.2-tmexD3.3-toprJ1b gene cluster, demonstrating that mcr-3.17 confers low-level resistance to colistin and tmexC3.2-tmexD3.3-toprJ1b confers low-level resistance to tigecycline.
Clinical and Bacterial Characteristics Associated with Glove and Gown Contamination by Carbapenem-Resistant Klebsiella pneumoniae in the Health Care Setting.
The study identified various carbapenem resistance genes such as bla KPC, bla KPC-3, bla KPC-2, bla NDM, and bla OXA-232, as well as aminoglycoside resistance genes like aph(6)-Id, aph(3″)-Ib, and others. It also found sulfonamide resistance genes (sul1, sul2, sul3), beta-lactam resistance genes (bla TEM, bla OXA), and quinolone resistance genes (qnrS1).
Clinical and Bacterial Characteristics Associated with Glove and Gown Contamination by Carbapenem-Resistant Klebsiella pneumoniae in the Health Care Setting.
The study identified various carbapenem resistance genes such as bla KPC, bla KPC-3, bla KPC-2, bla NDM, and bla OXA-232, as well as aminoglycoside resistance genes like aph(6)-Id, aph(3″)-Ib, and others. It also found sulfonamide resistance genes (sul1, sul2, sul3), beta-lactam resistance genes (bla TEM, bla OXA), and quinolone resistance genes (qnrS1).
Clinical and Bacterial Characteristics Associated with Glove and Gown Contamination by Carbapenem-Resistant Klebsiella pneumoniae in the Health Care Setting.
The study identified various carbapenem resistance genes such as bla KPC, bla KPC-3, bla KPC-2, bla NDM, and bla OXA-232, as well as aminoglycoside resistance genes like aph(6)-Id, aph(3″)-Ib, and others. It also found sulfonamide resistance genes (sul1, sul2, sul3), beta-lactam resistance genes (bla TEM, bla OXA), and quinolone resistance genes (qnrS1).
The carbapenem inoculum effect provides insight into the molecular mechanisms underlying carbapenem resistance in Enterobacterales.
The study identified that various carbapenemase genes, including blaKPC-3, blaSME-2, blaIMP-4, blaNDM-1, blaVIM-27, blaCMY-10, and blaOXA-48, confer a meropenem inoculum effect when expressed in E. coli K-12. These genes were experimentally validated to show increased resistance with higher inocula.
Isolation of Hv-CRKP with co-production of three carbapenemases (bla(KPC), bla(OXA-181) or (OXA-232), and bla(NDM-1)) and a virulence plasmid: a study from a Chinese tertiary hospital.
The study identifies the co-production of three carbapenemases (bla(KPC), bla(OXA-181) or (OXA-232), and bla(NDM-1)) in hypervirulent carbapenem-resistant Klebsiella pneumoniae (Hv-CRKP) isolates, highlighting the emergence of multidrug-resistant strains with hypervirulence traits.
Wastewater treatment plants, an "escape gate" for ESCAPE pathogens.
The study identifies several AMR genes and mutations in various pathogens found in wastewater treatment plants, highlighting the role of these facilities as reservoirs for antibiotic-resistant bacteria and genes.
Genomic Characteristics of a Multidrug-Resistant ST648 Escherichia coli Isolate Co-Carrying bla(KPC-2) and bla(CTX-M-15) Genes Recovered from a Respiratory Infection in China.
The study identifies a multidrug-resistant ST648 Escherichia coli isolate carrying bla(KPC-2) and bla(CTX-M-15) genes, along with other resistance genes such as tet(B), mdf(A), mph(A), dfrA17, aadA5, and sul1.
Emergence of ST463 exoU-Positive, Imipenem-Nonsusceptible Pseudomonas aeruginosa Isolates in China.
The study identified bla PDC, bla OXA-50, bla OXA-50-like, bla KPC-2, and bla OXA-486 as key resistance genes in imipenem-nonsusceptible Pseudomonas aeruginosa isolates. Additionally, mutations in PBP3 (F533L) and disruptions in oprD were associated with resistance. The ST463 lineage was highlighted as a multidrug-resistant and hypervirulent strain.
Whole-Genome Sequencing Revealed the Fusion Plasmids Capable of Transmission and Acquisition of Both Antimicrobial Resistance and Hypervirulence Determinants in Multidrug-Resistant Klebsiella pneumoniae Isolates.
The study identified multiple antimicrobial resistance genes and hypervirulence determinants in multidrug-resistant Klebsiella pneumoniae isolates, highlighting the role of fusion plasmids in the transmission of these traits.
Large-scale analysis of putative plasmids in clinical multidrug-resistant Escherichia coli isolates from Vietnamese patients.
The study identified several beta-lactamase genes, including bla KPC-2, bla NDM-5, bla OXA-1, bla OXA-48, and bla OXA-181, which are associated with carbapenem resistance in multidrug-resistant E. coli isolates from Vietnam.
Modulation of Klebsiella pneumoniae Outer Membrane Vesicle Protein Cargo under Antibiotic Treatment.
The study identified several AMR genes in the XDR K. pneumoniae strain HCD1, including beta-lactamases (blaTEM-1, blaKPC-2, blaCTX-M-15), an aminoglycoside modifying enzyme (aac(6')-Ib-cr), and other resistance determinants, highlighting the role of outer membrane vesicles in resistance dissemination.
Coexistence of bla(IMP-4), bla(NDM-1) and bla(OXA-1) in bla(KPC-2)-producing Citrobacter freundii of clinical origin in China.
The study identifies the coexistence of bla(IMP-4), bla(NDM-1), bla(OXA-1), and bla(KPC-2) in a multidrug-resistant Citrobacter freundii isolate, highlighting the complex mechanisms of carbapenem resistance.
Antimicrobial activity of cinnamaldehyde against multidrug-resistant Klebsiella pneumoniae: an in vitro and in vivo study.
The study identified the bla KPC-2 gene and mutations in the mgrB gene as contributors to carbapenem and polymyxin resistance in multidrug-resistant Klebsiella pneumoniae strains.
Multiplicity of bla(KPC) Genes and pKpQIL Plasmid Plasticity in the Development of Ceftazidime-Avibactam and Meropenem Coresistance in Klebsiella pneumoniae Sequence Type 307.
The study identifies blaKPC-31 and blaKPC-3 as key genes responsible for ceftazidime-avibactam and meropenem resistance in Klebsiella pneumoniae ST307, highlighting the role of plasmid plasticity in resistance development.
Multiplicity of bla(KPC) Genes and pKpQIL Plasmid Plasticity in the Development of Ceftazidime-Avibactam and Meropenem Coresistance in Klebsiella pneumoniae Sequence Type 307.
The study identifies blaKPC-31 and blaKPC-3 as key genes responsible for ceftazidime-avibactam and meropenem resistance in Klebsiella pneumoniae ST307, highlighting the role of plasmid plasticity in resistance development.
Expansion of healthcare-associated hypervirulent KPC-2-producing Klebsiella pneumoniae ST11/KL64 beyond hospital settings.
Two carbapenem-resistant K. pneumoniae strains (PINH-4250 and PINH-4900) were recovered from the Pinheiros River, carrying the blaKPC-2 carbapenemase gene and other resistance determinants. They exhibited a broad resistome and hypervirulent behavior.
Propranolol restores susceptibility of XDR Gram-negative pathogens to meropenem and Meropenem combination has been evaluated with either tigecycline or amikacin.
The study identifies blaKPC, blaOXA-48, and blaVIM as the主要 carbapenemase genes in XDR CR-GNB isolates, which contribute to resistance against meropenem.
Characterization of the two tandem repeats for the KPC-2 core structures on a plasmid from hospital-derived Klebsiella pneumoniae.
The study characterizes the tandem repeats of two KPC-2 core structures on a plasmid from hospital-derived Klebsiella pneumoniae, highlighting the role of these repeats in carbapenem resistance.
Global emergence of carbapenem-resistant Klebsiella pneumoniae co-carrying multiple carbapenemases.
The study identifies the global emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) strains co-carrying multiple carbapenemases, particularly bla NDM-1 and bla KPC-2. These strains show increased resistance to carbapenems and other antibiotics, highlighting the need for further research on the mechanisms behind multiple carbapenemase gene occurrences.
Genomics and Antimicrobial Susceptibility of Clinical Pseudomonas aeruginosa Isolates from Hospitals in Brazil.
The study identified several AMR genes, including blaCTX-M-2, blaKPC-2, and tetG, in P. aeruginosa isolates from Brazil. These genes conferred resistance to various beta-lactam and tetracycline antibiotics.
The optimized carbapenem inactivation method for objective and accurate detection of carbapenemase-producing Acinetobacter baumannii.
The study identifies and characterizes several carbapenemase-encoding genes, including bla TEM, bla SHV, bla KPC, bla OXA-23-like, and bla OXA-51-like, in carbapenem-resistant Acinetobacter baumannii isolates.
Carbapenem resistance in Enterobacterales bloodstream infections among children with cancer or post-haematopoietic stem cell transplant: a retrospective cohort study.
The study identified bla KPC and bla NDM as the primary carbapenem resistance mechanisms in Enterobacterales bloodstream infections among children with cancer or post-HSCT. These genes were experimentally validated using Xpert ® Carba-R.
Surface water in Lower Saxony: A reservoir for multidrug-resistant Enterobacterales.
The study identified multidrug-resistant Enterobacterales in surface water in Lower Saxony, including carbapenemase genes bla KPC-2, bla VIM-1, and bla OXA-181, as well as the colistin resistance gene mcr-9. It also discovered the novel ESBL gene bla CTX-M-32 in surface water.
Comparing antimicrobial resistant genes and phenotypes across multiple sequencing platforms and assays for Enterobacterales clinical isolates.
The study compared antimicrobial resistance genes and phenotypes across different sequencing platforms and assays for Enterobacterales clinical isolates, highlighting the detection of various AMR genes such as blaKPC-2, qnrB, oqxA, oqxB, sul1, sul2, drfA*, aac(3)-IIe, and blaCTX-M-1, which conferred resistance to multiple antibiotics.
Clonal dissemination of Klebsiella pneumoniae resistant to cefiderocol, ceftazidime/avibactam, meropenem/vaborbactam and imipenem/relebactam co-producing KPC and OXA-181 carbapenemase.
The study identifies KPC and OXA-181 carbapenemase co-producing Klebsiella pneumoniae strains resistant to multiple β-lactam/β-lactamase inhibitor combinations and cefiderocol, highlighting the clonal dissemination of these multidrug-resistant strains.
Clonal dissemination of Klebsiella pneumoniae resistant to cefiderocol, ceftazidime/avibactam, meropenem/vaborbactam and imipenem/relebactam co-producing KPC and OXA-181 carbapenemase.
The study identifies KPC and OXA-181 carbapenemase co-producing Klebsiella pneumoniae strains resistant to multiple β-lactam/β-lactamase inhibitor combinations and cefiderocol, highlighting the clonal dissemination of these multidrug-resistant strains.
Clonal dissemination of Klebsiella pneumoniae resistant to cefiderocol, ceftazidime/avibactam, meropenem/vaborbactam and imipenem/relebactam co-producing KPC and OXA-181 carbapenemase.
The study identifies KPC and OXA-181 carbapenemase co-producing Klebsiella pneumoniae strains resistant to multiple β-lactam/β-lactamase inhibitor combinations and cefiderocol, highlighting the clonal dissemination of these multidrug-resistant strains.
Clonal dissemination of Klebsiella pneumoniae resistant to cefiderocol, ceftazidime/avibactam, meropenem/vaborbactam and imipenem/relebactam co-producing KPC and OXA-181 carbapenemase.
The study identifies KPC and OXA-181 carbapenemase co-producing Klebsiella pneumoniae strains resistant to multiple β-lactam/β-lactamase inhibitor combinations and cefiderocol, highlighting the clonal dissemination of these multidrug-resistant strains.
Clonal dissemination of Klebsiella pneumoniae resistant to cefiderocol, ceftazidime/avibactam, meropenem/vaborbactam and imipenem/relebactam co-producing KPC and OXA-181 carbapenemase.
The study identifies KPC and OXA-181 carbapenemase co-producing Klebsiella pneumoniae strains resistant to multiple β-lactam/β-lactamase inhibitor combinations and cefiderocol, highlighting the clonal dissemination of these multidrug-resistant strains.
Clonal dissemination of Klebsiella pneumoniae resistant to cefiderocol, ceftazidime/avibactam, meropenem/vaborbactam and imipenem/relebactam co-producing KPC and OXA-181 carbapenemase.
The study identifies KPC and OXA-181 carbapenemase co-producing Klebsiella pneumoniae strains resistant to multiple β-lactam/β-lactamase inhibitor combinations and cefiderocol, highlighting the clonal dissemination of these multidrug-resistant strains.
Screening for Resistant Bacteria, Antimicrobial Resistance Genes, Sexually Transmitted Infections and Schistosoma spp. in Tissue Samples from Predominantly Vaginally Delivered Placentae in Ivory Coast and Ghana.
The study identified multiple beta-lactamase genes, including bla CTX-M, bla IMP, bla GES, bla VIM, bla OXA-58-like, bla NDM, bla OXA-23-like, bla OXA-48-like, and bla KPC, in placental tissue samples from Ivory Coast and Ghana. These genes confer resistance to various beta-lactam antibiotics, indicating a high prevalence of antimicrobial resistance in the studied region.
Neonatal Bloodstream Infection with Ceftazidime-Avibactam-Resistant bla(KPC-2)-Producing Klebsiella pneumoniae Carrying bla(VEB-25).
The study identifies bla(KPC-2) and bla(VEB-25) as resistance genes in a neonatal bloodstream infection caused by Klebsiella pneumoniae, highlighting the importance of molecular diagnostics in detecting novel resistance mechanisms.
Impact of acquired broad-spectrum β-lactamases on susceptibility to oral penems/carbapenems (tebipenem, sulopenem, and faropenem) alone or in combination with avibactam and taniborbactam β-lactamase inhibitors in Escherichia coli.
The study evaluates the impact of various β-lactamases on the susceptibility of Escherichia coli to oral penems/carbapenems (tebipenem, sulopenem, and faropenem) and their combinations with β-lactamase inhibitors avibactam and taniborbactam.
Impact of acquired broad-spectrum beta-lactamases on susceptibility to oral penems/carbapenems (tebipenem, sulopenem, and faropenem) alone or in combination with avibactam and taniborbactam beta-lactamase inhibitors in Escherichia coli.
The study evaluates the impact of various beta-lactamases on the susceptibility of Escherichia coli to oral penems/carbapenems (tebipenem, sulopenem, and faropenem) and their combinations with beta-lactamase inhibitors avibactam and taniborbactam.
Dynamic evolution of ceftazidime-avibactam resistance due to interchanges between bla(KPC-2) and bla(KPC-145) during treatment of Klebsiella pneumoniae infection.
The study reports the first case of CZA resistance due to the blaKPC-145 gene, which emerged during CZA treatment of a KPC-2-producing K. pneumoniae infection. The blaKPC-145 gene conferred resistance to ceftazidime/avibactam and restored susceptibility to carbapenems.
Dynamic evolution of ceftazidime-avibactam resistance due to interchanges between bla(KPC-2) and bla(KPC-145) during treatment of Klebsiella pneumoniae infection.
Dynamic evolution of ceftazidime-avibactam resistance due to interchanges between bla(KPC-2) and bla(KPC-145) during treatment of Klebsiella pneumoniae infection.
Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America.
Two novel KPC variants, KPC-113 and KPC-114, were identified in ceftazidime-avibactam-resistant Klebsiella pneumoniae strains belonging to high-risk clones ST11 and ST16 in South America.
Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America.
Two novel KPC variants, KPC-113 and KPC-114, were identified in ceftazidime-avibactam-resistant Klebsiella pneumoniae strains belonging to high-risk clones ST11 and ST16 in South America.
CpxR promotes the carbapenem antibiotic resistance of Klebsiella pneumoniae by directly regulating the expression and the dissemination of bla(KPC) on the IncFII conjugative plasmid.
CpxR directly regulates the expression and dissemination of bla(KPC) on the IncFII conjugative plasmid, enhancing carbapenem resistance in Klebsiella pneumoniae.
Emergence of Tigecycline and Carbapenem-Resistant Citrobacter freundii Co-Carrying tmexCD1-toprJ1, bla(KPC-2), and bla(NDM-1) from a Sepsis Patient.
The study identifies the first report of a tigecycline and carbapenem-resistant Citrobacter freundii strain, CF10, carrying tmexCD1-toprJ1, bla(KPC-2), and bla(NDM-1).
Characterization of ST11 and ST15 Carbapenem-Resistant Hypervirulent Klebsiella pneumoniae from Patients with Ventilator-Associated Pneumonia.
The study identifies blaKPC-2, blaOXA-232, and blaNDM-1 as the主要 carbapenemase genes in hv-CRKP isolates, highlighting their role in carbapenem resistance.
Antimicrobial resistance, pathogenic potential, and genomic features of carbapenem-resistant Klebsiella pneumoniae isolated in Chile: high-risk ST25 clones and novel mobile elements.
The study identified multiple carbapenemase genes, including bla KPC-2, bla NDM-1, and bla NDM-7, as well as other resistance genes such as bla CTX-M-15, armA, mph(2')-I, and brp(MBL). Mutations in ompK35, ompK36, gyrA, parC, pmrB, and mgrB were associated with resistance to various antibiotics.
Pipeline validation for the identification of antimicrobial-resistant genes in carbapenem-resistant Klebsiella pneumoniae.
The study validated a bioinformatics pipeline for identifying antimicrobial resistance genes in carbapenem-resistant K. pneumoniae, focusing on the detection of bla KPC-2 as the sole carbapenem resistance gene.
Pseudomonas aeruginosa High-Risk Sequence Type 463 Co-Producing KPC-2 and AFM-1 Carbapenemases, China, 2020-2022.
The study reports the clonal spread of Pseudomonas aeruginosa sequence type 463 co-producing KPC-2 and AFM-1 carbapenemases in China, highlighting their extensive drug resistance, disinfectant and desiccation resilience, and strong biofilm formation capabilities.
Rapid Detection of bla(KPC) in Carbapenem-Resistant Enterobacterales Based on CRISPR/Cas13a.
The study developed a rapid RPA-Cas13a assay for detecting the bla(KPC) gene, which encodes a carbapenemase responsible for carbapenem resistance in Enterobacterales.
Rapid Detection of bla(KPC) in Carbapenem-Resistant Enterobacterales Based on CRISPR/Cas13a.
The study developed a rapid RPA-Cas13a assay for detecting the bla(KPC) gene, which encodes a carbapenemase responsible for carbapenem resistance in Enterobacterales.
Exploring avibactam and relebactam inhibition of Klebsiella pneumoniae carbapenemase D179N variant: role of the Ω loop-held deacylation water.
The study characterizes the D179N variant of KPC-2 beta-lactamase and its interaction with avibactam and relebactam, highlighting the role of the Ω loop in resistance mechanisms.
Characterization of Beta-Lactam Resistome of Escherichia coli Causing Nosocomial Infections.
The study characterized the beta-lactam resistome of Escherichia coli causing nosocomial infections, identifying several beta-lactamase genes including blaTEM, blaCTX, blaSHV, blaBIL, blaDHA, blaCMY, blaIMP, blaLAP, blaP, blaVIM, and blaKPC, which confer resistance to various beta-lactam antibiotics.
Characterization of Beta-Lactam Resistome of Escherichia coli Causing Nosocomial Infections.
The study characterized the beta-lactam resistome of Escherichia coli causing nosocomial infections, identifying several beta-lactamase genes including blaTEM, blaCTX, blaSHV, blaBIL, blaDHA, blaCMY, blaIMP, blaLAP, blaP, blaVIM, and blaKPC, which confer resistance to various beta-lactam antibiotics.
Isolation and Characterisation of Human-Derived bla(KPC-3)-Producing Salmonella enterica Serovar Rissen in 2018.
The study identifies a Salmonella enterica serovar Rissen strain carrying the blaKPC-3 gene, which confers resistance to meropenem, imipenem, and ertapenem.
Characterization of Carbapenemase- and ESBL-Producing Gram-Negative Bacilli Isolated from Patients with Urinary Tract and Bloodstream Infections.
The study identified multiple carbapenemase and ESBL genes, including bla KPC-2, bla KPC-3, bla NDM, bla CTX-M-15, bla CTX-M-27, bla CTX-M-14, bla SHV-187, bla SHV-12, bla OXA-181, and others, highlighting the diversity of beta-lactam resistance mechanisms in Gram-negative bacteria from urinary tract and bloodstream infections.
Characterization of Carbapenemase- and ESBL-Producing Gram-Negative Bacilli Isolated from Patients with Urinary Tract and Bloodstream Infections.
The study identified multiple carbapenemase and ESBL genes, including bla KPC-2, bla KPC-3, bla NDM, bla CTX-M-15, bla CTX-M-27, bla CTX-M-14, bla SHV-187, bla SHV-12, bla OXA-181, and others, highlighting the diversity of beta-lactam resistance mechanisms in Gram-negative bacteria from urinary tract and bloodstream infections.
Comparative Genomics Reveals Novel Species and Insights into the Biotechnological Potential, Virulence, and Resistance of Alcaligenes.
The study identifies multiple antimicrobial resistance genes in Alcaligenes species, particularly in clinical isolates, highlighting the presence of genes conferring resistance to β-lactams, aminoglycosides, sulfonamides, and other antibiotics.
A Whole-Genome Sequencing-Based Approach for the Characterization of Klebsiella pneumoniae Co-Producing KPC and OXA-48-like Carbapenemases Circulating in Sardinia, Italy.
The study identified K. pneumoniae strains co-producing KPC and OXA-48-like carbapenemases, with blaKPC-31 and blaKPC-3 variants showing resistance to carbapenems and beta-lactams, alongside porin mutations affecting carbapenem susceptibility.
A Whole-Genome Sequencing-Based Approach for the Characterization of Klebsiella pneumoniae Co-Producing KPC and OXA-48-like Carbapenemases Circulating in Sardinia, Italy.
The study identified K. pneumoniae strains co-producing KPC and OXA-48-like carbapenemases, with blaKPC-31 and blaKPC-3 variants showing resistance to carbapenems and beta-lactams, alongside porin mutations affecting carbapenem susceptibility.
Emergence of KPC-134, a KPC-2 variant associated with ceftazidime-avibactam resistance in a ST11 Klebsiella pneumoniae clinical strain.
The study reports the first characterization of a novel KPC variant, KPC-134, which confers resistance to ceftazidime-avibactam and restores susceptibility to imipenem in a ST11 Klebsiella pneumoniae clinical strain.
Secondary Infection Surveillance with Metagenomic Next-Generation Sequencing in COVID-19 Patients: A Cross-Sectional Study.
The study identified several AMR genes, including blaKPC, blaSHV, blaCTX-M, blaTEM, blaOXA-23, blaOXA-51, blaNDM, mecA, and ErmB, which confer resistance to various antibiotics in clinically relevant pathogens.
SHIP: identifying antimicrobial resistance gene transfer between plasmids.
SHIP identifies horizontally transferred antimicrobial resistance (AMR) gene regions between plasmids, including a multi-resistant complex class 1 integron in Escherichia coli and Klebsiella pneumoniae, and a tetracycline resistance region in Enterococcus faecalis.
In vitro potency of xeruborbactam in combination with multiple β-lactam antibiotics in comparison with other β-lactam/β-lactamase inhibitor (BLI) combinations against carbapenem-resistant and extended-spectrum β-lactamase-producing Enterobacterales.
Xeruborbactam (XER) showed superior in vitro potency against carbapenem-resistant and extended-spectrum β-lactamase-producing Enterobacterales when combined with various β-lactam antibiotics compared to other β-lactam/β-lactamase inhibitor combinations. XER effectively inhibited a wide range of β-lactamases, including metallo-β-lactamases (MBLs) and serine β-lactamases, enhancing the activity of antibiotics such as meropenem, cefepime, ceftolozane, ceftriaxone, aztreonam, piperacillin, and ertapenem.
In vitro potency of xeruborbactam in combination with multiple β-lactam antibiotics in comparison with other β-lactam/β-lactamase inhibitor (BLI) combinations against carbapenem-resistant and extended-spectrum β-lactamase-producing Enterobacterales.
Xeruborbactam (XER) showed superior in vitro potency against carbapenem-resistant and extended-spectrum β-lactamase-producing Enterobacterales when combined with various β-lactam antibiotics compared to other β-lactam/β-lactamase inhibitor combinations. XER effectively inhibited a wide range of β-lactamases, including metallo-β-lactamases (MBLs) and serine β-lactamases, enhancing the activity of antibiotics such as meropenem, cefepime, ceftolozane, ceftriaxone, aztreonam, piperacillin, and ertapenem.
bla (KPC-2) overexpression and bla (GES-5) carriage as major imipenem/relebactam resistance mechanisms in Pseudomonas aeruginosa high-risk clones ST463 and ST235, respectively, in China.
The study identifies blaKPC-2 overexpression and blaGES-5 carriage as major mechanisms of imipenem/relebactam resistance in Pseudomonas aeruginosa high-risk clones ST463 and ST235, respectively.
Clinical and molecular epidemiology of carbapenem-resistant Enterobacteriaceae in pediatric inpatients in South China.
The study identified blaNDM-5, blaNDM-1, blaIMP-4, and blaKPC-2 as the most prevalent carbapenemase genes in CRE isolates from pediatric inpatients in South China.
KPC-2-producing Pseudomonas aeruginosa isolated from wild animals in Brazil.
The study identified the blaKPC-2 gene in Pseudomonas aeruginosa isolates from wild animals in Brazil, highlighting the presence of this carbapenem-resistance gene in a wild environment.
Genomics analysis of KPC-2 and NDM-5-producing Enterobacteriaceae in migratory birds from Qinghai Lake, China.
The study identified bla KPC-2 and bla NDM-5 genes in Enterobacteriaceae from migratory birds in Qinghai Lake, China, highlighting the role of these birds in the spread of carbapenem-resistant strains.
Genotypic Evolution of Klebsiella pneumoniae Sequence Type 512 during Ceftazidime/Avibactam, Meropenem/Vaborbactam, and Cefiderocol Treatment, Italy.
The study identifies a novel KPC-154 beta-lactamase and a nonsense mutation in the cirA gene contributing to cefiderocol resistance in Klebsiella pneumoniae ST512. Additionally, a deletion in the ompK36 gene was linked to reduced meropenem/vaborbactam susceptibility.
Genotypic Evolution of Klebsiella pneumoniae Sequence Type 512 during Ceftazidime/Avibactam, Meropenem/Vaborbactam, and Cefiderocol Treatment, Italy.
Genotypic Evolution of Klebsiella pneumoniae Sequence Type 512 during Ceftazidime/Avibactam, Meropenem/Vaborbactam, and Cefiderocol Treatment, Italy.
Multicenter evaluation of the BIOFIRE Joint Infection Panel for the detection of bacteria, yeast, and AMR genes in synovial fluid samples.
The BIOFIRE Joint Infection Panel demonstrated high sensitivity and specificity for detecting bacteria and antimicrobial resistance (AMR) genes in synovial fluid samples. It effectively identified various AMR genes, including mecA/C and MREJ (MRSA), vanA/B, CTX-M, OXA-48-like, IMP, KPC, NDM, and VIM, in multiple bacterial species.
Rapid Isolation of Low-Level Carbapenem-Resistant E. coli from Water and Foods Using Glycan-Coated Magnetic Nanoparticles.
The study demonstrates the use of glycan-coated magnetic nanoparticles (gMNPs) for the rapid and cost-effective extraction of carbapenem-resistant E. coli (KPC and NDM producers) from water and food samples.
Prioritization of Critical Factors for Surveillance of the Dissemination of Antibiotic Resistance in Pseudomonas aeruginosa: A Systematic Review.
The study identifies 25 critical mobile antibiotic resistance genes (ARGs) in Pseudomonas aeruginosa, including genes such as sul1, qacEΔ1, aac(6′)-Ib, bla VIM-1, and others, which are associated with various antibiotic classes and are linked to mobile genetic elements (MGEs).
Molecular characteristics and antimicrobial resistance profiles of Carbapenem-Resistant Klebsiella pneumoniae isolates at a tertiary hospital in Nanning, China.
The study identified NDM-1, NDM-5, KPC-2, OXA-181, and IMP-4 as the primary carbapenemase genes in CRKP isolates, with NDM-1 being the most prevalent.
Co-carriage of Plasmid NDM and Chromosomal KPC in Klebsiella pneumoniae ST255 Human Wound Isolate in Brazil.
The study reports a multidrug-resistant Klebsiella pneumoniae ST255 isolate carrying chromosomal blaKPC and plasmid-mediated blaNDM and blaOXA-9, highlighting the co-carriage of multiple β-lactamase genes.
A dual-process of targeted and unbiased Nanopore sequencing enables accurate and rapid diagnosis of lower respiratory infections.
The study identified several AMR genes, including blaOXA-50, blaOXA-66, blaOXA-23, blaKPC-2, and mecA, which confer resistance to various antibiotics in different bacterial species.
High prevalence of antibiotic resistance and biofilm formation in Salmonella Gallinarum.
The study identified multiple antibiotic resistance genes in Salmonella Gallinarum isolates, including GES, IMP, VIM, NDM, SHV, KPC, FOX, qnrB, qnrS, and sdiA, which are associated with resistance to various antibiotics. Additionally, all isolates were found to form biofilms, indicating a potential link between biofilm formation and antibiotic resistance.
Dissemination and characteristics of carbapenem-resistant Klebsiella pneumoniae in nine district hospitals in southwestern China.
The study identified carbapenem-resistant Klebsiella pneumoniae (CRKP) strains in nine district hospitals in Chongqing, China, with high levels of resistance to carbapenem antibiotics. The primary resistance mechanisms involved carbapenemase production, including bla KPC, bla NDM, and bla IMP genes, as well as co-expression of multiple carbapenemases. The study also highlighted the presence of hypervirulent CRKP (HV-CRKP) strains.
Infection with Carbapenem-resistant Hypervirulent Klebsiella Pneumoniae: clinical, virulence and molecular epidemiological characteristics.
The study identifies bla_kpc-2, bla_ndm-1, and bla_oxa-48 as the主要 carbapenemase genes in CR-hvKP isolates, with bla_kpc-2 being the most prevalent. It also highlights the association of ST11 with CR-hvKP and the increased risk factors for infection such as tracheal intubation and intracranial infection.
Aromatic Diboronic Acids as Effective KPC/AmpC Inhibitors.
The study identified ortho-phenylenediboronic acid 3a as an effective inhibitor of KPC-3 beta-lactamase, enhancing the efficacy of carbapenems against KPC-producing bacteria.
Epidemiology and characterization of Providencia stuartii isolated from hospitalized patients in southern Brazil: a possible emerging pathogen.
The study identified the presence of bla CTX-M-2, bla CTX-M-9, and bla KPC-2 genes in Providencia stuartii isolates, which confer resistance to multiple antibiotics, including beta-lactams and carbapenems.
Decoding the genetic structure of conjugative plasmids in international clones of Klebsiella pneumoniae: A deep dive into blaKPC, blaNDM, blaOXA-48, and blaGES genes.
The study characterizes the genetic structure of plasmids harboring major carbapenemase genes (blaKPC, blaNDM, blaOXA-48, and blaGES) in Klebsiella pneumoniae, identifying the most prevalent allele types and their co-occurrence with other resistance genes.
Klebsiella pneumoniae TolC contributes to antimicrobial resistance, exopolysaccellar production, and virulence.
TolC is required for antimicrobial resistance, capsule production, and virulence in K. pneumoniae. Mutations in tolC lead to increased susceptibility to multiple antibiotics and impair capsule production and biofilm formation.
Involvement of Tn3 transposon in formation and transmission of hypervirulent and carbapenem-resistant Klebsiella pneumoniae.
The study identifies the blaKPC-2 gene as a key factor in carbapenem resistance in hypervirulent Klebsiella pneumoniae, highlighting the role of Tn3 transposon in the formation and transmission of resistance and virulence traits.
Evaluation of Antibiotic Resistance Mechanisms in Gram-Negative Bacteria.
The paper discusses various antibiotic resistance mechanisms in Gram-negative bacteria, highlighting the role of beta-lactamases, aminoglycoside-modifying enzymes, and efflux pumps in conferring resistance to multiple antibiotics.
Evaluation of Antibiotic Resistance Mechanisms in Gram-Negative Bacteria.
The paper discusses various antibiotic resistance mechanisms in Gram-negative bacteria, highlighting the role of beta-lactamases, aminoglycoside-modifying enzymes, and efflux pumps in conferring resistance to multiple antibiotics.
Klebsiella pneumoniae carbapenemase variant 44 acquires ceftazidime-avibactam resistance by altering the conformation of active-site loops.
The KPC-44 variant of the Klebsiella pneumoniae carbapenemase (KPC) confers resistance to ceftazidime/avibactam (CAZ-AVI) by altering the conformation of active-site loops, resulting in reduced AVI carbamylation efficiency and increased MIC values compared to KPC-2.
Klebsiella pneumoniae carbapenemase variant 44 acquires ceftazidime-avibactam resistance by altering the conformation of active-site loops.
The KPC-44 variant of the Klebsiella pneumoniae carbapenemase (KPC) confers resistance to ceftazidime/avibactam (CAZ-AVI) by altering the conformation of active-site loops, resulting in reduced AVI carbamylation efficiency and increased MIC values compared to KPC-2.
The impact of three carbapenems at a single-day dose on intestinal colonization resistance against carbapenem-resistant Klebsiella pneumoniae.
The study identifies blaKPC-2 as a carbapenem-resistance gene in CRKP strain 140731, demonstrating its role in conferring resistance to carbapenems.
Molecular Epidemiology of Carbapenem Resistant Klebsiella Pneumoniae in Northern China: Clinical Characteristics, Antimicrobial Resistance, Virulence and Geographic Distribution.
The study identified several carbapenemase genes, including bla NDM-1, bla NDM-5, bla KPC-2, bla OXA-23, bla SME, and bla IMI, as well as extended-spectrum beta-lactamase genes such as bla CTX-M, bla SHV, and bla TEM, and AmpC beta-lactamase gene bla DHA, which contribute to carbapenem resistance in CRKP isolates from Shanxi Province.
Within-Host Resistance and Virulence Evolution of a Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae ST11 Under Antibiotic Pressure.
The study identified the acquisition of the rmtB gene as a mechanism for aminoglycoside resistance in hv-CRKP strains, and internal mutations in rmpA and rmpA2 genes were linked to reduced hypermucoviscosity and virulence.
Mobilization of the bla(KPC-14) gene among heterogenous plasmids in extensively drug-resistant hypervirulent Klebsiella pneumoniae.
The study identifies the bla(KPC-14) gene as a significant contributor to ceftazidime/avibactam, meropenem, and ertapenem resistance in extensively drug-resistant hypervirulent Klebsiella pneumoniae. The gene was found on non-conjugative IncFII/IncR plasmids and could be transferred to E. coli through conjugation experiments.
Clinical and molecular characteristics of Klebsiella pneumoniae infection in a tertiary general hospital of Wuhan, China.
The study identified bla KPC-2 as a major carbapenem resistance gene in Klebsiella pneumoniae isolates, along with virulence genes rmpA, rmpA2, iroN, and iucA. ST11- KPC-2 was responsible for most of the K. pneumoniae infections.
Comparison of the inoculum effect of in vitro antibacterial activity of Imipenem/relebactam and Ceftazidime/avibactam against ESBL-, KPC- and AmpC-producing Escherichia coli and Klebsiella pneumoniae.
The study evaluated the inoculum effect of Imipenem/relebactam (IMR) and Ceftazidime/avibactam (CZA) against ESBL-, KPC-, and AmpC-producing E. coli and K. pneumoniae. It identified several beta-lactamase genes, including bla TEM-1, bla AmpC, bla CTX-M-14, bla OXA-10, bla CTX-M-55, bla CMY-42, bla CTX-M-15, bla KPC-2, bla OXA-9, bla SHV-182, bla SHV-18, bla OXA-2, bla OKP-B-7, bla DHA-1, bla SHV-27, bla CTX-M-65, bla SHV-11, bla SHV-145, bla SHV-12, bla SHV-106, bla SHV-110, bla SHV106, and bla CTX-M-14, which confer resistance to ceftazidime and/or imipenem.
Predominant transmission of KPC-2 carbapenemase in Germany by a unique IncN plasmid variant harboring a novel non-transposable element (NTE (KPC) -Y).
The study identifies a unique IncN[pMLST15] plasmid variant harboring a novel non-transposable element (NTE KPC-Y) that carries the bla KPC-2 gene along with other antibiotic resistance genes, contributing to the widespread transmission of KPC-2 carbapenemase in Germany.
Microbiological profile of patients treated for postoperative peritonitis: temporal trends 1999-2019.
The study identifies the presence of carbapenemase genes (blaVIM, blaKPC, blaOXA-48, blaNDM, and blaIMP) in Escherichia coli isolates, indicating resistance to carbapenems. It also notes an increase in multidrug-resistant Enterobacterales, particularly ESBL-producing strains, over time.
Comparative Meropenem Pharmacodynamics and Emergence of Resistance against Carbapenem-Susceptible Non-Carbapenemase-Producing and Carbapenemase-Producing Enterobacterales: A Pharmacodynamic Study in a Hollow-Fiber Infection Model.
The study identified that meropenem-susceptible carbapenemase-producing strains, such as those carrying blaOXA-48 and blaKPC-2, may not respond effectively to meropenem therapy, highlighting the importance of considering carbapenemase production in susceptibility testing.
Contamination of a Water Stream and Water Drainage Reaching Matosinhos Beach by Antibiotic-Resistant Bacteria.
The study identified carbapenemase-producing bacteria, including Escherichia coli and KESC group, carrying bla KPC and bla CTX-M-group1 genes, which confer resistance to meropenem and extended-spectrum beta-lactams.
Clinical, microbiological and genomic characterization of Gram-negative bacteria with dual carbapenemases as identified by rapid molecular testing.
The study characterizes dual carbapenemase-producing organisms (DCPOs) and identifies various carbapenemase genes including bla NDM, bla OXA-48-like, and bla KPC. These genes were found to confer resistance to carbapenems and were located on distinct plasmids, except for one case where both bla NDM and bla KPC were on a single plasmid.
A preliminary exploration on the mechanism of the carbapenem-resistance transformation of Serratia marcescens in vivo.
The study identifies the mechanisms of carbapenem resistance in Serratia marcescens, highlighting the role of bla KPC gene acquisition, increased expression of bla KPC, and loss of a porin gene leading to higher expression of bla CTX-M-14.
Descriptive analysis of targeted carbapenemase genes and antibiotic susceptibility profiles among carbapenem-resistant Acinetobacter baumannii tested in the Antimicrobial Resistance Laboratory Network-United States, 2017-2020.
The study analyzed carbapenem-resistant Acinetobacter baumannii (CRAB) isolates and identified the presence of various carbapenemase genes, including bla KPC, bla NDM, bla OXA-23-like, and bla OXA-24/40-like. These genes contribute to extensive drug resistance in CRAB isolates.
Detection of clinically relevant antibiotic-resistant bacteria in shared fomites, waste water and municipal solid wastes disposed near residential areas of a Nigerian city.
The study identified several antibiotic resistance genes (ARGs) including bla KPC, bla NDM-1, bla CMY-2, and bla IMP in various environmental samples. It also detected two variants of the bla OXA-51-like gene (bla OXA-66 and bla OXA-180) in Acinetobacter baumannii. Additionally, methicillin-resistant Staphylococcus aureus (MRSA) and extended-spectrum beta-lactamase (ESBL)-producing bacteria were found in sewage, sludge, and shared fomites.
A growing battlefield in the war against biofilm-induced antimicrobial resistance: insights from reviews on antibiotic resistance.
The review discusses various mechanisms of biofilm-induced antimicrobial resistance, including the role of efflux pumps such as AcrAB-TolC, MexAB-OprM, AdeFGH, and AcrD in conferring resistance to multiple classes of antibiotics.
Metagenomic identification of pathogens and antimicrobial-resistant genes in bacterial positive blood cultures by nanopore sequencing.
The study identified various antimicrobial resistance (AMR) genes in bacterial positive blood cultures using nanopore sequencing, demonstrating the effectiveness of this method in detecting resistance mechanisms and pathogens quickly.
Emergence of KPC-2 and NDM-5-coproducing hypervirulent carbapenem-resistant Klebsiella pneumoniae with high-risk sequence types ST11 and ST15.
The study identifies two hypervirulent carbapenem-resistant Klebsiella pneumoniae isolates, FK3122 and FK3127, that co-harbored bla KPC-2, bla NDM-5, and virulence plasmids, demonstrating high-level resistance to multiple antibiotics and significant pathogenicity.
Genome sequencing unveils bla(KPC-2)-harboring plasmids as drivers of enhanced resistance and virulence in nosocomial Klebsiella pneumoniae.
The study identifies blaKPC-2, blaSHV-12, and blaCTX-M-65 as key genes contributing to carbapenem and ceftazidime/avibactam resistance in nosocomial Klebsiella pneumoniae, highlighting the role of plasmid-mediated resistance and gene amplification in clinical settings.
Concurrent transmission of multiple carbapenemases in a long-term acute-care hospital.
The study identified bla VIM and bla KPC genes in Pseudomonas aeruginosa and various Enterobacterales, respectively, contributing to carbapenem resistance in a long-term acute-care hospital setting.
Proteomic analysis of meropenem-induced outer membrane vesicles released by carbapenem-resistant Klebsiella pneumoniae.
The study identifies the blaKPC-2 gene as a key determinant of carbapenem resistance in a clinical isolate of carbapenem-resistant Klebsiella pneumoniae (CRKP).
Clinical use of tigecycline may contribute to the widespread dissemination of carbapenem-resistant hypervirulent Klebsiella pneumoniae strains.
The study identified the bla KPC-2 gene as a critical factor in carbapenem resistance in Klebsiella pneumoniae, highlighting its role in the dissemination of carbapenem-resistant hypervirulent strains.
Drug Discovery in the Field of β-Lactams: An Academic Perspective.
This review discusses the current state of beta-lactamase inhibitor research, focusing on novel chemotypes and their mechanisms of action against various beta-lactamase classes, highlighting the importance of structure-based design and the challenges in overcoming resistance.
The mechanism of ceftazidime and cefiderocol hydrolysis by D179Y variants of KPC carbapenemases is similar and involves the formation of a long-lived covalent intermediate.
The study characterizes the D179Y variants of KPC carbapenemases (KPC-31 and KPC-33) and shows that they confer resistance to ceftazidime and cefiderocol by altering the rate-limiting step of the hydrolysis mechanism, leading to the formation of a long-lived covalent intermediate.
The mechanism of ceftazidime and cefiderocol hydrolysis by D179Y variants of KPC carbapenemases is similar and involves the formation of a long-lived covalent intermediate.
The study characterizes the D179Y variants of KPC carbapenemases (KPC-31 and KPC-33) and shows that they confer resistance to ceftazidime and cefiderocol by altering the rate-limiting step of the hydrolysis mechanism, leading to the formation of a long-lived covalent intermediate.
The mechanism of ceftazidime and cefiderocol hydrolysis by D179Y variants of KPC carbapenemases is similar and involves the formation of a long-lived covalent intermediate.
The study characterizes the D179Y variants of KPC carbapenemases (KPC-31 and KPC-33) and shows that they confer resistance to ceftazidime and cefiderocol by altering the rate-limiting step of the hydrolysis mechanism, leading to the formation of a long-lived covalent intermediate.
The mechanism of ceftazidime and cefiderocol hydrolysis by D179Y variants of KPC carbapenemases is similar and involves the formation of a long-lived covalent intermediate.
The study characterizes the D179Y variants of KPC carbapenemases (KPC-31 and KPC-33) and shows that they confer resistance to ceftazidime and cefiderocol by altering the rate-limiting step of the hydrolysis mechanism, leading to the formation of a long-lived covalent intermediate.
Applicability of Bronchoalveolar Lavage Fluid and Plasma Metagenomic Next-Generation Sequencing Assays in the Diagnosis of Pneumonia.
The study evaluated the applicability of bronchoalveolar lavage fluid (BALF) and plasma metagenomic next-generation sequencing (mNGS) assays in diagnosing pneumonia and detecting antibiotic resistance genes. It found that BALF mNGS had high sensitivity but lower specificity for pneumonia diagnosis, and identified resistance genes such as bla KPC and bla NDM in Klebsiella pneumoniae.
Multiplex Microarrays in 96-Well Plates Photoactivated with 4-Azidotetrafluorobenzaldehyde for the Identification and Quantification of beta-lactamase Genes and Their RNA Transcripts.
The study developed a novel microarray technique using photoactivated 96-well plates to identify and quantify beta-lactamase genes and their RNA transcripts. The method successfully detected various beta-lactamase genes, including ESBLs, inhibitor-resistant beta-lactamases, and carbapenemases, demonstrating high specificity and reproducibility.
Enhancing the activity of beta-lactamase inhibitory protein-II with cell-penetrating peptide against KPC-2-carrying Klebsiella pneumoniae.
The study demonstrates that tBLIP-II and tBLIP-II-CPP effectively inhibit KPC-2 carbapenemase, significantly reducing the MIC of meropenem against KPC-2-positive Klebsiella pneumoniae.
Molecular Characteristics and Antimicrobial Susceptibility Profiles of bla(KPC)-Producing Escherichia Coli Isolated from a Teaching Hospital in Shanghai, China.
The study identified blaKPC as a gene responsible for carbapenem resistance in E. coli isolates from a teaching hospital in Shanghai, China. The gene was found to be associated with various plasmid types and was linked to Tn4401 structures.
KPC-2 allelic variants in Klebsiella pneumoniae isolates resistant to ceftazidime-avibactam from Argentina: bla(KPC-80), bla(KPC-81), bla(KPC-96) and bla(KPC-97).
The study identifies four novel KPC-2 allelic variants (bla KPC-80, bla KPC-81, bla KPC-96, and bla KPC-97) in ceftazidime-avibactam-resistant Klebsiella pneumoniae isolates from Argentina, which confer resistance to multiple beta-lactam antibiotics.
KPC-2 allelic variants in Klebsiella pneumoniae isolates resistant to ceftazidime-avibactam from Argentina: bla(KPC-80), bla(KPC-81), bla(KPC-96) and bla(KPC-97).
The study identifies four novel KPC-2 allelic variants (bla KPC-80, bla KPC-81, bla KPC-96, and bla KPC-97) in ceftazidime-avibactam-resistant Klebsiella pneumoniae isolates from Argentina, which confer resistance to multiple beta-lactam antibiotics.
KPC-2 allelic variants in Klebsiella pneumoniae isolates resistant to ceftazidime-avibactam from Argentina: bla(KPC-80), bla(KPC-81), bla(KPC-96) and bla(KPC-97).
The study identifies four novel KPC-2 allelic variants (bla KPC-80, bla KPC-81, bla KPC-96, and bla KPC-97) in ceftazidime-avibactam-resistant Klebsiella pneumoniae isolates from Argentina, which confer resistance to multiple beta-lactam antibiotics.
KPC-2 allelic variants in Klebsiella pneumoniae isolates resistant to ceftazidime-avibactam from Argentina: bla(KPC-80), bla(KPC-81), bla(KPC-96) and bla(KPC-97).
The study identifies four novel KPC-2 allelic variants (bla KPC-80, bla KPC-81, bla KPC-96, and bla KPC-97) in ceftazidime-avibactam-resistant Klebsiella pneumoniae isolates from Argentina, which confer resistance to multiple beta-lactam antibiotics.
KPC-2 allelic variants in Klebsiella pneumoniae isolates resistant to ceftazidime-avibactam from Argentina: bla(KPC-80), bla(KPC-81), bla(KPC-96) and bla(KPC-97).
The study identifies four novel KPC-2 allelic variants (bla KPC-80, bla KPC-81, bla KPC-96, and bla KPC-97) in ceftazidime-avibactam-resistant Klebsiella pneumoniae isolates from Argentina, which confer resistance to multiple beta-lactam antibiotics.
Antimicrobial resistance in aeromonads and new therapies targeting quorum sensing.
The paper discusses the prevalence of antimicrobial resistance in Aeromonas species, highlighting the presence of various beta-lactamase genes such as blaTEM-24, blaIMP-19, blaVIM-4, blaKPC-2, blaNDM-1, blaVIM-2, blaOXA-48, blaIMP-13, blaGES-5, blaTEM-1, blaSHV-12, blaVEB-9, blaMOX, blaFOX, blaACC, and others. It also identifies genes like cphA, vat, mcr-3.41, mcr-7.1, sul, dfr, tetA, rsmA, and adeF associated with resistance to sulfonamides, trimethoprim, tetracycline, polymyxin, and other antibiotics. The study emphasizes the role of horizontal gene transfer and mobile genetic elements in the dissemination of these resistance genes.
ESKAPE in China: epidemiology and characteristics of antibiotic resistance.
The study characterizes various AMR genes and mutations in ESKAPE pathogens in China, highlighting the prevalence of carbapenem-resistant K. pneumoniae (CRKP) and the emergence of resistance mechanisms such as blaKPC-2, blaNDM-1, blaNDM-5, rmtB, armA, fosA3, optrA, and poxtA.
The Biofilm Lifestyle Shapes the Evolution of beta-lactamases.
The study identifies mutations in the beta-lactamase KPC-2 that reverse its inhibitory effect on biofilm formation in Vibrio cholerae while maintaining ampicillin resistance.
Difference analysis and characteristics of incompatibility group plasmid replicons in gram-negative bacteria with different antimicrobial phenotypes in Henan, China.
The study identified bla KPC and bla NDM as the primary carbapenemase genes associated with carbapenem-resistant organisms (CROs) in Henan, China. These genes were frequently found on plasmid replicons such as IncFII, IncR, IncX3, and IncFIB, highlighting their role in the spread of carbapenem resistance.
Whole genome sequence-based molecular characterization of blood isolates of carbapenem-resistant Enterobacter cloacae complex from ICU patients in Kolkata, India, during 2017-2022: emergence of phylogenetically heterogeneous Enterobacter hormaechei subsp. xiangfangensis.
The study identified several carbapenemase and extended-spectrum beta-lactamase genes, including bla NDM-1, bla NDM-4, bla NDM-5, bla NDM-7, bla OXA-181, bla OXA-232, bla KPC-3, bla CTX-M-15, bla SFO-1, bla ACT, and bla CMH-3, in carbapenem-resistant Enterobacter cloacae complex isolates from Kolkata, India. Novel integrons (In180, In4874, In4887, and In4888) were also discovered.
Comparison of the Antibiotic Resistance of Escherichia coli Populations from Water and Biofilm in River Environments.
The study identified several beta-lactamase genes, including bla CTX-M-15, bla CTX-M-1, bla CTX-M-14, bla SHV-12, bla TEM-1, and bla KPC-2, which confer resistance to various antibiotics in E. coli isolates from river environments.
Bioactive Compounds from Plant Origin as Natural Antimicrobial Agents for the Treatment of Wound Infections.
The review discusses the antimicrobial activity of plant-derived compounds such as berberine, catechin, chelerythrine, cinnamaldehyde, ellagic acid, proanthocyanidin, and sanguinarine against various bacteria, highlighting their potential as natural antimicrobial agents.
In-depth characterization of multidrug-resistant NDM-1 and KPC-3 co-producing Klebsiella pneumoniae bloodstream isolates from Italian hospital patients.
The study characterized multidrug-resistant NDM-1 and KPC-3 co-producing Klebsiella pneumoniae bloodstream isolates, identifying several AMR genes and mutations associated with resistance to various antibiotics.
Genomic insights and antimicrobial resistance profiles of CRKP and non-CRKP isolates in a Beijing geriatric medical center: emphasizing the bla(KPC-2) carrying high-risk clones and their spread.
The study identifies blaKPC-2 as a key gene in carbapenem resistance among CRKP isolates, along with other resistance genes such as rmtB, APH(3')-Ia, and QnrB4. It also highlights the prevalence of ST11-KL47-OL101 clones and the role of plasmid pKpnR03_2 in the spread of resistance.
Evolution of ceftazidime-avibactam and cefiderocol resistance in ST131-H30R1-Escherichia coli isolates with KPC-3 mutants and application of FTIR biotyping.
Evolution of ceftazidime-avibactam and cefiderocol resistance in ST131-H30R1-Escherichia coli isolates with KPC-3 mutants and application of FTIR biotyping.
Evolution of ceftazidime-avibactam and cefiderocol resistance in ST131-H30R1-Escherichia coli isolates with KPC-3 mutants and application of FTIR biotyping.
Biofilm formation and antimicrobial resistance pattern of uropathogenic E. coli ST131 isolated from children with malignant tumors.
The study identified carbapenem resistance genes blaVIM, blaNDM, blaKPC, and blaIMP in E. coli ST131 isolates, along with biofilm-related genes lasR, pelA, and lecA. ST131 isolates showed higher antimicrobial resistance and biofilm-forming capabilities compared to non-ST131 isolates.
Carbapenemase-producing Enterobacterales strains causing infections in companion animals-Portugal.
The study identified five carbapenemase-producing Enterobacterales strains in companion animals in Portugal, including three KPC-3-producing K. pneumoniae, one OXA-181-producing K. pneumoniae, and one OXA-48-producing E. coli. These strains were found to carry resistance genes on various plasmids, highlighting the potential for horizontal transmission of carbapenem resistance between human and animal settings.
Prospective observational pilot study of the T2Resistance panel in the T2Dx system for detection of resistance genes in bacterial bloodstream infections.
The T2Resistance panel effectively detected various resistance genes in bacterial bloodstream infections, including bla KPC, bla NDM /bla IMP /bla VIM, bla CTXM-14/15, bla AmpC, and mec A/ mec C, demonstrating high sensitivity and rapid detection times.
Carbapenem-resistant hypervirulent ST23 Klebsiella pneumoniae with a highly transmissible dual-carbapenemase plasmid in Chile.
The study identifies a novel dual-carbapenemase plasmid in a hypervirulent ST23 Klebsiella pneumoniae strain from Chile, encoding KPC-2 and VIM-1 carbapenemases, which confer resistance to multiple beta-lactam antibiotics.
First report of multidrug-resistant carbapenemase-producing Aeromonas caviae co-harboring mcr-3.43 and mcr-7.2.
The study reports the first identification of mcr-3.43 and mcr-7.2, along with bla NDM-1 and bla KPC-2, in a single Aeromonas caviae strain from hospital sewage, highlighting the potential for multidrug resistance in this genus.
Prevalence and characteristics of ertapenem-mono-resistant isolates among carbapenem-resistant Enterobacterales in China.
The study identified carbapenemase genes (bla KPC, bla NDM, bla IMP-4, bla OXA-181, and bla VIM-1) and porin mutations contributing to ertapenem-mono-resistance in carbapenem-resistant Enterobacterales (CRE) in China. ETP-mono-resistant CRE strains showed lower carbapenemase positivity and higher susceptibility to certain antibiotics compared to MEM/IPM-resistant strains.
Activity of Epsilon-poly-L-lysine against Multidrug-Resistant Pseudomonas aeruginosa and Klebsiella pneumoniae Isolates of Urinary Tract Infections.
The study identified various beta-lactamase genes, including blaSPM, blaKPC, blaSHV, blaCTX-M, blaOXA, blaTEM, blaPER, blaVIM, and blaVIM-2 in Pseudomonas aeruginosa, and blaCTX-M, blaTEM, blaKPC, blaNDM, and blaOXA in Klebsiella pneumoniae. Additionally, aac(3)-IV, aadA1, aac(3)-II, sul2, sul1, sul3, dfrA, cmlA, and tetA were found to confer resistance to aminoglycosides, sulfonamides, trimethoprim, chloramphenicol, and tetracyclines.
Effect of Temperature on Carbapenemase-Encoding Plasmid Transfer in Klebsiella pneumoniae.
The study shows that the transfer of plasmids carrying blaKPC and blaNDM genes in Klebsiella pneumoniae is influenced by temperature, with optimal conjugation temperatures of 25°C for blaKPC and 30°C for blaNDM.
Molecular characterization of hybrid virulence plasmids in ST11-KL64 KPC-2-producing multidrug-resistant hypervirulent Klebsiella pneumoniae from China.
The study identified two novel hybrid virulence plasmids in ST11-KL64 KPC-2-producing multidrug-resistant hypervirulent Klebsiella pneumoniae strains, which co-harbored blaKPC-2 and virulence factors, contributing to carbapenem resistance and enhanced virulence.
Genomic insights into the evolution and mechanisms of carbapenem-resistant hypervirulent Klebsiella pneumoniae co-harboring bla(KPC) and bla(NDM): implications for public health threat mitigation.
The study identified bla(KPC) and bla(NDM) genes in carbapenem-resistant hypervirulent Klebsiella pneumoniae strains, highlighting their role in resistance and the potential for horizontal gene transfer through hybrid plasmids.
Emergence of pandrug-resistant carbapenemase-producing Enterobacterales in dogs and cats: a cross-sectional study in Egypt.
The study identified multiple carbapenemase genes, including bla_OXA-181, bla_IMP, bla_OXA-48-like, bla_KPC, bla_VIM, and bla_NDM, in carbapenem-resistant Enterobacterales isolated from dogs and cats in Egypt.
Characteristics and phylogenetic distribution of megaplasmids and prediction of a putative chromid in Pseudomonas aeruginosa.
The study identifies multiple beta-lactamase genes, including blaIMP-45, blaVIM-2, and blaKPC, which confer resistance to carbapenems in Pseudomonas aeruginosa megaplasmids.
Characteristics and phylogenetic distribution of megaplasmids and prediction of a putative chromid in Pseudomonas aeruginosa.
The study identifies multiple beta-lactamase genes, including blaIMP-45, blaVIM-2, and blaKPC, which confer resistance to carbapenems in Pseudomonas aeruginosa megaplasmids.
Characteristics and phylogenetic distribution of megaplasmids and prediction of a putative chromid in Pseudomonas aeruginosa.
The study identifies multiple beta-lactamase genes, including blaIMP-45, blaVIM-2, and blaKPC, which confer resistance to carbapenems in Pseudomonas aeruginosa megaplasmids.
Hybrid Sequencing-Based Genomic Analysis of Klebsiella pneumoniae from Urinary Tract Infections Among Inpatients at a Tertiary Hospital in Beijing.
The study identified the blaKPC-2 gene in 35% of K. pneumoniae strains, which confers resistance to imipenem and meropenem. Additionally, an insertion of an IS5-like sequence in the mgrB gene was found to increase resistance to Polymyxin B.
Molecular characterization and descriptive analysis of carbapenemase-producing Gram-negative rod infections in Bogota, Colombia.
The study identified blaKPC-2 and blaKPC-3 as the most prevalent carbapenemase-encoding genes in carbapenem-resistant Gram-negative rods in Bogota, Colombia. These genes were frequently found in co-occurrence with blaVIM-2 and blaNDM-1 in healthcare-acquired infections.
Molecular characterization and descriptive analysis of carbapenemase-producing Gram-negative rod infections in Bogota, Colombia.
The study identified blaKPC-2 and blaKPC-3 as the most prevalent carbapenemase-encoding genes in carbapenem-resistant Gram-negative rods in Bogota, Colombia. These genes were frequently found in co-occurrence with blaVIM-2 and blaNDM-1 in healthcare-acquired infections.
Proton pump inhibitors increase the risk of carbapenem-resistant Enterobacteriaceae colonization by facilitating the transfer of antibiotic resistance genes among bacteria in the gut microbiome.
The study identifies that PPIs increase the risk of CRE colonization by enhancing the transfer of carbapenem resistance genes (CRGs) among gut bacteria, particularly highlighting the role of KPC and OXA carbapenemases.
The emergence of highly resistant and hypervirulent Klebsiella pneumoniae CC14 clone in a tertiary hospital over 8 years.
The study identifies the emergence of highly resistant and hypervirulent Klebsiella pneumoniae ST2096 clone carrying bla OXA-48 and bla OXA-232 genes on plasmids, along with the iuc locus for hypervirulence.
Biodiversity of carbapenem-resistant bacteria in clinical samples from the Southwest Amazon region (Rondônia/Brazil).
The study identified various carbapenemase-encoding genes, including bla KPC -like, bla NDM -like, bla OXA-23 -like, bla OXA-58 -like, bla OXA-143 -like, bla OXA-48 -like, bla SPM -like, bla VIM -like, and bla IMP -like, in carbapenem-resistant bacteria from Rondônia, Brazil.
Detection of bla(KPC) gene among carbapenemase producing Klebsiella pneumoniae isolated from different clinical specimens at tertiary care hospital of Nepal.
The study detected the blaKPC gene in 8.3% of meropenem-resistant Klebsiella pneumoniae isolates, highlighting the presence of carbapenemase-producing strains in Nepal.
Antimicrobial activity of ceftazidime-avibactam against KPC-2-producing Enterobacterales: a cross-combination and dose-escalation titration study with relebactam and vaborbactam.
The study found that ceftazidime-avibactam was highly effective against KPC-2-producing Enterobacterales, with only 1.1% of isolates showing resistance. The presence of KPC-2 was the primary determinant of resistance to carbapenems.
Deciphering the Coevolutionary Dynamics of L2 beta-lactamases via Deep Learning.
The study identifies and characterizes several L2 beta-lactamase variants (L2a, L2b, L2c, L2d) along with SME-1 and KPC-2, highlighting their structural and functional dynamics in relation to β-lactam resistance.
First report of KPC variants conferring ceftazidime-avibactam resistance in Colombia: introducing KPC-197.
The study reports the first occurrence of KPC-197, a novel variant of the KPC carbapenemase, which confers resistance to ceftazidime-avibactam in Colombia. Additionally, KPC-31 was identified in another isolate.
First report of KPC variants conferring ceftazidime-avibactam resistance in Colombia: introducing KPC-197.
The study reports the first occurrence of KPC-197, a novel variant of the KPC carbapenemase, which confers resistance to ceftazidime-avibactam in Colombia. Additionally, KPC-31 was identified in another isolate.
First report of KPC variants conferring ceftazidime-avibactam resistance in Colombia: introducing KPC-197.
First report of KPC variants conferring ceftazidime-avibactam resistance in Colombia: introducing KPC-197.
Emergence of carbapenem-resistant Pseudomonas aeruginosa ST179 producing both IMP-16 and KPC-2: a case study of introduction from Peru to Spain.
The study reports the emergence of carbapenem-resistant Pseudomonas aeruginosa ST179 carrying blaIMP-16, blaKPC-2, and blaKPC-35 genes, highlighting the global threat of multidrug-resistant clones and the importance of monitoring resistance mechanisms.
Emergence of carbapenem-resistant Pseudomonas aeruginosa ST179 producing both IMP-16 and KPC-2: a case study of introduction from Peru to Spain.
The study reports the emergence of carbapenem-resistant Pseudomonas aeruginosa ST179 carrying blaIMP-16, blaKPC-2, and blaKPC-35 genes, highlighting the global threat of multidrug-resistant clones and the importance of monitoring resistance mechanisms.
Assessment of three antibiotic combination regimens against Gram-negative bacteria causing neonatal sepsis in low- and middle-income countries.
The study identifies multiple AMR genes and mutations in Gram-negative bacteria causing neonatal sepsis in LMICs, including ESBLs, carbapenemases, and aminoglycoside-modifying enzymes, highlighting the high prevalence of multidrug resistance and the need for effective antibiotic combinations.
Sporadic clone Escherichia coli ST615 as a vector and reservoir for dissemination of crucial antimicrobial resistance genes.
The study identifies several antimicrobial resistance genes, including bla CTX-M-15, bla KPC-2, bla NDM-5, bla NDM-1, aadB, and mcr-1, which were successfully acquired by the sporadic clone Escherichia coli ST615 through conjugation and transformation.
Long-term occurrence of multiple antimicrobial drug resistant Klebsiella pneumoniae isolates harboring virulent potential in a tertiary hospital from Brazil.
The study identified multiple antimicrobial resistance genes, including bla KPC, bla OXA-1-like, and bla CTX-M-8, in Klebsiella pneumoniae isolates from a Brazilian hospital. These isolates also exhibited virulence factors and multidrug-resistant (MDR) and extensively drug-resistant (XDR) phenotypes.
In vitro mimicry of in vivo KPC mutations by ceftazidime-avibactam: phenotypes, mechanisms, genetic structure and kinetics of enzymatic hydrolysis.
The study characterizes several KPC variants (bla KPC-25, bla KPC-127, bla KPC-100, bla KPC-128, bla KPC-136, bla KPC-137, bla KPC-144, and bla KPC-180) that emerge during ceftazidime-avibactam treatment, demonstrating their role in conferring resistance to ceftazidime-avibactam and altering carbapenem susceptibility.
In vitro mimicry of in vivo KPC mutations by ceftazidime-avibactam: phenotypes, mechanisms, genetic structure and kinetics of enzymatic hydrolysis.
The study characterizes several KPC variants (bla KPC-25, bla KPC-127, bla KPC-100, bla KPC-128, bla KPC-136, bla KPC-137, bla KPC-144, and bla KPC-180) that emerge during ceftazidime-avibactam treatment, demonstrating their role in conferring resistance to ceftazidime-avibactam and altering carbapenem susceptibility.
In vitro mimicry of in vivo KPC mutations by ceftazidime-avibactam: phenotypes, mechanisms, genetic structure and kinetics of enzymatic hydrolysis.
The study characterizes several KPC variants (bla KPC-25, bla KPC-127, bla KPC-100, bla KPC-128, bla KPC-136, bla KPC-137, bla KPC-144, and bla KPC-180) that emerge during ceftazidime-avibactam treatment, demonstrating their role in conferring resistance to ceftazidime-avibactam and altering carbapenem susceptibility.
In vitro mimicry of in vivo KPC mutations by ceftazidime-avibactam: phenotypes, mechanisms, genetic structure and kinetics of enzymatic hydrolysis.
The study characterizes several KPC variants (bla KPC-25, bla KPC-127, bla KPC-100, bla KPC-128, bla KPC-136, bla KPC-137, bla KPC-144, and bla KPC-180) that emerge during ceftazidime-avibactam treatment, demonstrating their role in conferring resistance to ceftazidime-avibactam and altering carbapenem susceptibility.
In vitro mimicry of in vivo KPC mutations by ceftazidime-avibactam: phenotypes, mechanisms, genetic structure and kinetics of enzymatic hydrolysis.
The study characterizes several KPC variants (bla KPC-25, bla KPC-127, bla KPC-100, bla KPC-128, bla KPC-136, bla KPC-137, bla KPC-144, and bla KPC-180) that emerge during ceftazidime-avibactam treatment, demonstrating their role in conferring resistance to ceftazidime-avibactam and altering carbapenem susceptibility.
In vitro mimicry of in vivo KPC mutations by ceftazidime-avibactam: phenotypes, mechanisms, genetic structure and kinetics of enzymatic hydrolysis.
The study characterizes several KPC variants (bla KPC-25, bla KPC-127, bla KPC-100, bla KPC-128, bla KPC-136, bla KPC-137, bla KPC-144, and bla KPC-180) that emerge during ceftazidime-avibactam treatment, demonstrating their role in conferring resistance to ceftazidime-avibactam and altering carbapenem susceptibility.
In vitro mimicry of in vivo KPC mutations by ceftazidime-avibactam: phenotypes, mechanisms, genetic structure and kinetics of enzymatic hydrolysis.
The study characterizes several KPC variants (bla KPC-25, bla KPC-127, bla KPC-100, bla KPC-128, bla KPC-136, bla KPC-137, bla KPC-144, and bla KPC-180) that emerge during ceftazidime-avibactam treatment, demonstrating their role in conferring resistance to ceftazidime-avibactam and altering carbapenem susceptibility.
In vitro mimicry of in vivo KPC mutations by ceftazidime-avibactam: phenotypes, mechanisms, genetic structure and kinetics of enzymatic hydrolysis.
The study characterizes several KPC variants (bla KPC-25, bla KPC-127, bla KPC-100, bla KPC-128, bla KPC-136, bla KPC-137, bla KPC-144, and bla KPC-180) that emerge during ceftazidime-avibactam treatment, demonstrating their role in conferring resistance to ceftazidime-avibactam and altering carbapenem susceptibility.
In vitro mimicry of in vivo KPC mutations by ceftazidime-avibactam: phenotypes, mechanisms, genetic structure and kinetics of enzymatic hydrolysis.
The study characterizes several KPC variants (bla KPC-25, bla KPC-127, bla KPC-100, bla KPC-128, bla KPC-136, bla KPC-137, bla KPC-144, and bla KPC-180) that emerge during ceftazidime-avibactam treatment, demonstrating their role in conferring resistance to ceftazidime-avibactam and altering carbapenem susceptibility.
Patient outcomes by baseline pathogen resistance phenotype and genotype in CERTAIN-1, a Phase 3 study of cefepime-taniborbactam versus meropenem in adults with complicated urinary tract infection.
The study characterizes various AMR genes and mutations in Enterobacterales and Pseudomonas aeruginosa, including bla CTX-M-15, bla OXA-1, bla OXA-181, bla OXA-48, bla NDM-1, bla KPC-3, bla VIM-2, ampC, cmrA, mexAB-OprM, mexXY-OprM, oprD, ompK35, ompK36, and ftsI, which confer resistance to cefepime and carbapenems.
Carbapenem-Resistant and Extended-Spectrum β-Lactamase-Producing Enterobacterales in Children, United States, 2016-2020.
The study identified bla NDM, bla OXA-48-like, and bla KPC genes as the primary carbapenemase producers in carbapenem-resistant Enterobacterales (CRE) cases among children in the United States from 2016 to 2020.
Molecular mechanisms responsible KPC-135-mediated resistance to ceftazidime-avibactam in ST11-K47 hypervirulent Klebsiella pneumoniae.
The study identifies KPC-135, a novel KPC variant with a deletion in the Ω-loop and a tandem repeat in the 270-loop, which confers resistance to ceftazidime-avibactam.
Comparison of three colloidal gold immunoassays and GeneXpert Carba-R for the detection of Klebsiella pneumoniae blaKPC-2 variants.
The study evaluates the performance of three colloidal gold immunoassays and GeneXpert Carba-R in detecting KPC-2 and its variants, showing that GeneXpert Carba-R is highly effective in detecting all KPC-2 variants.
Characterizing carbapenemase-producing Escherichia coli isolates from Spain: high genetic heterogeneity and wide geographical spread.
The study identifies various carbapenemase genes such as bla OXA-48, bla VIM-1, bla NDM-1, bla KPC-3, and bla NDM-5 in carbapenem-resistant E. coli isolates from Spain, highlighting their genetic diversity and geographic distribution.
Molecular Prevalence and Geographical Variations of Carbapenem-Resistant Klebsiella pneumoniae ST15 Isolates in a Tertiary Hospital in Ningbo, China.
The study identified blaKPC-2 as the primary carbapenem resistance gene in 14 out of 18 ST15 CRKP isolates, highlighting its significance in carbapenem resistance within this population.
A comparative evaluation of five phenotypic methods for identification of carbapenemase-producing Enterobacteriaceae: a modified carbapenemase detection test.
The study evaluates five phenotypic methods for detecting carbapenemase-producing Enterobacteriaceae, highlighting the modified Carba NP (mCNP) test as having the highest sensitivity (95.06%) for carbapenemase detection.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
The prevalence and mechanisms of heteroresistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
Prevalence of ST1049-KL5 carbapenem-resistant Klebsiella pneumoniae with a bla(KPC-2) and bla(NDM-1) co-carrying hypertransmissible IncM1 plasmid.
The study identifies a hypertransmissible IncM1 plasmid, pKPC_NDM, co-carrying bla KPC-2 and bla NDM-1 genes in ST1049-KL5 KPC_NDM_CRKP isolates, highlighting its role in carbapenem resistance and efficient transferability between bacterial hosts.
Prevalence of ST1049-KL5 carbapenem-resistant Klebsiella pneumoniae with a bla(KPC-2) and bla(NDM-1) co-carrying hypertransmissible IncM1 plasmid.
The study identifies a hypertransmissible IncM1 plasmid, pKPC_NDM, co-carrying bla KPC-2 and bla NDM-1 genes in ST1049-KL5 KPC_NDM_CRKP isolates, highlighting its role in carbapenem resistance and efficient transferability between bacterial hosts.
Tracing the possible evolutionary trends of Morganella morganii: insights from molecular epidemiology and phylogenetic analysis.
The study identified several antimicrobial resistance genes in Morganella morganii, including bla KPC-2, mcr-1, tet(X4), and tmexCD-toprJ. Additionally, a novel bla KPC-2-bearing plasmid was discovered, highlighting the role of mobile genetic elements in the spread of resistance.
Genomic and immunocyte characterisation of bloodstream infection caused by Klebsiella pneumoniae.
The study identifies blaKPC as a major carbapenemase gene in CRKP isolates and highlights the association of clpV, ybtQ, and qacE with increased 30-day mortality in BSI-Kpn patients.
Complete Genome Sequence of a Klebsiella pneumoniae Strain Carrying Novel Variant bla(KPC-203), Cross-Resistant to Ceftazidime/Avibactam and Cefiderocol, but Susceptible to Carbapenems, Isolated in Italy, 2023.
The study identified a novel KPC variant, KPC-203, which confers resistance to ceftazidime/avibactam and cefiderocol but maintains susceptibility to carbapenems in a Klebsiella pneumoniae strain.
Complete Genome Sequence of a Klebsiella pneumoniae Strain Carrying Novel Variant bla(KPC-203), Cross-Resistant to Ceftazidime/Avibactam and Cefiderocol, but Susceptible to Carbapenems, Isolated in Italy, 2023.
Complete Genome Sequence of a Klebsiella pneumoniae Strain Carrying Novel Variant bla(KPC-203), Cross-Resistant to Ceftazidime/Avibactam and Cefiderocol, but Susceptible to Carbapenems, Isolated in Italy, 2023.
Detection of KPC-216, a Novel KPC-3 Variant, in a Clinical Isolate of Klebsiella pneumoniae ST101 Co-Resistant to Ceftazidime-Avibactam and Cefiderocol.
The study identifies a novel KPC-3 variant, KPC-216, in a clinical isolate of Klebsiella pneumoniae ST101 that shows resistance to ceftazidime-avibactam and cefiderocol.
Detection of KPC-216, a Novel KPC-3 Variant, in a Clinical Isolate of Klebsiella pneumoniae ST101 Co-Resistant to Ceftazidime-Avibactam and Cefiderocol.
Detection of KPC-216, a Novel KPC-3 Variant, in a Clinical Isolate of Klebsiella pneumoniae ST101 Co-Resistant to Ceftazidime-Avibactam and Cefiderocol.
Characteristic of KPC-12, a KPC Variant Conferring Resistance to Ceftazidime-Avibactam in the Carbapenem-Resistant Klebsiella pneumoniae ST11-KL47 Clone Background.
The study identifies KPC-12, a novel KPC variant in a carbapenem-resistant Klebsiella pneumoniae ST11-KL47 strain, which confers resistance to ceftazidime-avibactam. The variant shows reduced carbapenemase activity compared to KPC-2 and KPC-3 but exhibits increased resistance to CZA upon induction.
Molecular characterization and epidemiological investigation of colistin resistance in carbapenem-resistant Klebsiella pneumoniae in a tertiary care hospital in Tehran, Iran.
The study identified mcr-1 as a cause of colistin resistance in CRKP isolates and characterized multiple carbapenemase genes, including bla OXA−48, bla KPC, bla VIM, bla IMP, and bla NDM.
Detection of hidden antibiotic resistance through real-time genomics.
The study highlights the effectiveness of real-time genomics in detecting low-abundance plasmid-mediated antibiotic resistance, particularly the bla KPC-14 gene variant, which was missed by traditional diagnostics but identified through nanopore sequencing.
Detection of hidden antibiotic resistance through real-time genomics.
The study highlights the effectiveness of real-time genomics in detecting low-abundance plasmid-mediated antibiotic resistance, particularly the bla KPC-14 gene variant, which was missed by traditional diagnostics but identified through nanopore sequencing.
Co-colonization of different species harboring KPC or NDM carbapenemase in the same host gut: insight of resistance evolution by horizontal gene transfer.
The study identified blaNDM-5, blaNDM-1, and blaKPC-2 carbapenemase genes in 10 CRE isolates from five patients, demonstrating their transferability between different species in the gut through horizontal gene transfer.
Study of the Epidemiological and Mechanistic Differences Between Carbapenem-Resistant Klebsiella pneumoniae Infections in Children and Adults.
The study identifies blaNDM-5 and blaKPC-2 as the predominant carbapenemase genes in pediatric and adult CRKP strains, respectively. It also highlights differences in ESBL and AmpC gene profiles between the two groups.
A large-scale surveillance revealed that KPC variants mediated ceftazidime-avibactam resistance in clinically isolated Klebsiella pneumoniae.
The study identified KPC variants, including bla KPC-33, bla KPC-86, and a novel variant bla KPC-129, which mediate ceftazidime-avibactam resistance in Klebsiella pneumoniae.
A large-scale surveillance revealed that KPC variants mediated ceftazidime-avibactam resistance in clinically isolated Klebsiella pneumoniae.
The study identified KPC variants, including bla KPC-33, bla KPC-86, and a novel variant bla KPC-129, which mediate ceftazidime-avibactam resistance in Klebsiella pneumoniae.
A large-scale surveillance revealed that KPC variants mediated ceftazidime-avibactam resistance in clinically isolated Klebsiella pneumoniae.
The study identified KPC variants, including bla KPC-33, bla KPC-86, and a novel variant bla KPC-129, which mediate ceftazidime-avibactam resistance in Klebsiella pneumoniae.
A large-scale surveillance revealed that KPC variants mediated ceftazidime-avibactam resistance in clinically isolated Klebsiella pneumoniae.
The study identified KPC variants, including bla KPC-33, bla KPC-86, and a novel variant bla KPC-129, which mediate ceftazidime-avibactam resistance in Klebsiella pneumoniae.
Genomic characterization of Klebsiella pneumoniae carbapenemase-producing Klebsiella pneumoniae (KPC-Kp) strains circulating in three university hospitals in Northern Italy over three years.
The study identified blaKPC-2, blaKPC-3, and blaKPC-33 as the primary carbapenemase genes in KPC-Kp strains, along with porin mutations in ompK35 and ompK36 contributing to reduced susceptibility to ceftazidime/avibactam and meropenem. Additionally, mgrB and pmrB truncations were associated with increased colistin susceptibility.
Genomic characterization of Klebsiella pneumoniae carbapenemase-producing Klebsiella pneumoniae (KPC-Kp) strains circulating in three university hospitals in Northern Italy over three years.
The study identified blaKPC-2, blaKPC-3, and blaKPC-33 as the primary carbapenemase genes in KPC-Kp strains, along with porin mutations in ompK35 and ompK36 contributing to reduced susceptibility to ceftazidime/avibactam and meropenem. Additionally, mgrB and pmrB truncations were associated with increased colistin susceptibility.
Genomic characterization of Klebsiella pneumoniae carbapenemase-producing Klebsiella pneumoniae (KPC-Kp) strains circulating in three university hospitals in Northern Italy over three years.
The study identified blaKPC-2, blaKPC-3, and blaKPC-33 as the primary carbapenemase genes in KPC-Kp strains, along with porin mutations in ompK35 and ompK36 contributing to reduced susceptibility to ceftazidime/avibactam and meropenem. Additionally, mgrB and pmrB truncations were associated with increased colistin susceptibility.
Double bla(KPC-2) copies quadrupled minimum inhibitory concentration of ceftazidime-avibactam in hospital-derived Klebsiella pneumoniae.
The study identifies double copies of bla KPC-2 in a hospital-derived Klebsiella pneumoniae strain, which significantly increases the minimum inhibitory concentration of ceftazidime-avibactam.
Citrobacter spp. and Enterobacter spp. as reservoirs of carbapenemase blaNDM and blaKPC resistance genes in hospital wastewater.
The study identifies Citrobacter spp. and Enterobacter spp. as significant reservoirs of carbapenemase genes blaKPC and blaNDM in hospital wastewater, highlighting their role in the spread of antibiotic resistance.
Starvation resilience of Klebsiella species in oral and nasal microbiomes
The study identifies the presence of beta-lactamase genes in Klebsiella pneumoniae isolates from healthy human oral and nasal cavities, highlighting their multidrug resistance properties.
Antimicrobial Resistance in Carbapenem-Resistant Acinetobacter baumannii and Pseudomonas aeruginosa: Mechanisms, Spread, and Environmental Impacts
The paper discusses the prevalence of carbapenem-resistant Acinetobacter baumannii (CRAB) and Pseudomonas aeruginosa (CRPA) in hospital and municipal wastewater, highlighting the role of antibiotic resistance genes (ARGs) in the spread of carbapenem resistance. It emphasizes the environmental impact of antibiotic resistance and the need for better management strategies.
Performance of Flow Cytometry-Based Rapid Assay in Detection of Carbapenemase-Producing Enterobacterales.
The study evaluated a flow cytometry-based rapid assay for detecting and differentiating carbapenemase-producing Enterobacterales. It identified blaVIM-1, blaOXA-48, and blaKPC-2 as the most prevalent carbapenemase genes in the isolates analyzed.
Detection of Klebsiella pneumoniae Carbapenem Resistance Genes by qPCR: Choosing the Right Method for Total DNA Extraction.
The study evaluates various DNA extraction methods for the detection of carbapenem resistance genes in Klebsiella pneumoniae using qPCR, highlighting the importance of selecting the right method for reliable resistance gene detection.
16S rRNA amplicon sequencing and antimicrobial resistance profile of intensive care units environment in 41 Brazilian hospitals.
The study identified the frequent presence of antimicrobial resistance genes such as mecA, bla KPC-like, bla NDM-like, and bla OXA-23-like in ICU surfaces and sanitizers in Brazilian hospitals.
Correlation between Antibiotics-Resistance, Virulence Genes and Genotypes among Klebsiella pneumoniae Clinical Strains Isolated in Guangzhou, China.
The study identified carbapenemase genes KPC, VIM, and NDM, along with virulence genes entB, mrkD, ybtS, kfu, iutA, rmpA, and allS in Klebsiella pneumoniae clinical isolates. High resistance rates to penicillin and cephalosporins were observed, with KPC being the most prevalent carbapenemase gene.
Limited transmission of carbapenem-resistant Klebsiella pneumoniae between animals and humans: a study in Qingdao.
The study identifies the presence of carbapenem-resistant Klebsiella pneumoniae (CRKP) in hospital inpatients, farm animals, and meat products, highlighting the limited transmission of CRKP between animals and humans. It characterizes the carbapenemase genes blaNDM-1, blaNDM-5, and blaKPC-2, as well as the colistin resistance gene mcr-9, in various isolates.
Characterization of a KPC-84 harboring Klebsiella pneumoniae ST11 clinical isolate with ceftazidime-avibactam resistance.
The study identifies a novel KPC variant, KPC-84, in a Klebsiella pneumoniae ST11 clinical isolate from China, which exhibits resistance to ceftazidime-avibactam. KPC-84 shows slightly higher hydrolysis of ceftazidime and reduced affinity to avibactam compared to KPC-2, but restores susceptibility to carbapenems.
Characterization of GQA as a novel beta-lactamase inhibitor of CTX-M-15 and KPC-2 enzymes.
GQA was identified as a novel beta-lactamase inhibitor that effectively reduces the MICs of β-lactam antibiotics against strains producing CTX-M-15 and KPC-2 beta-lactamases.
Phenotypic and molecular characterization of multidrug-resistant Enterobacterales isolated from clinical samples in Palestine: a focus on extended-spectrum β-lactamase- and carbapenemase-producing isolates.
The study identified several AMR genes, including blaTEM, blaSHV, blaCTXm, blaNDM, blaOXA48, and blaKPC, which confer resistance to various beta-lactam antibiotics. These genes were detected in multidrug-resistant Enterobacterales isolates from clinical samples in Palestine.
Coexistence of plasmid-mediated tmexCD2-toprJ2, bla(IMP-4), and bla(NDM-1) in Klebsiella quasipneumoniae.
The study identifies the coexistence of plasmid-mediated tmexCD2-toprJ2, blaIMP-4, and blaNDM-1 in Klebsiella quasipneumoniae strain FK8966, highlighting the emergence of multidrug-resistant strains with resistance to tigecycline and carbapenems.
Proteomic analysis of carbapenem-resistant Klebsiella pneumoniae outer membrane vesicles under the action of phages combined with tigecycline.
The study identifies blaNDM-1 and blaKPC-2 as the primary carbapenem resistance genes in K. pneumoniae 0692, which were experimentally validated through PCR and whole-genome sequencing.
In vivo evolution to hypermucoviscosity and ceftazidime/avibactam resistance in a liver abscess caused by Klebsiella pneumoniae sequence type 512.
The study identifies a blaKPC-31 gene variant conferring resistance to ceftazidime/avibactam and a F557S mutation in the wzc gene associated with hypermucoviscosity in a Klebsiella pneumoniae ST512 strain.
Retrospective analysis of molecular characteristics, risk factors, and outcomes in carbapenem-resistant Klebsiella pneumoniae bloodstream infections.
The study identified blaKPC-2 and blaKPC-12 as the predominant carbapenemase genes in CRKP strains, contributing to carbapenem resistance. It also found that ST11 was the most prevalent sequence type among CRKP isolates.
Retrospective analysis of molecular characteristics, risk factors, and outcomes in carbapenem-resistant Klebsiella pneumoniae bloodstream infections.
The study identified blaKPC-2 and blaKPC-12 as the predominant carbapenemase genes in CRKP strains, contributing to carbapenem resistance. It also found that ST11 was the most prevalent sequence type among CRKP isolates.
Relationship between virulence and carbapenem resistance phenotype of Klebsiella pneumoniae from blood infection: identification of a carbapenem-resistant and hypervirulent strain.
The study identified a carbapenem-resistant and hypervirulent Klebsiella pneumoniae strain (CR-HVKP) carrying various resistance genes including bla KPC-2, bla SHV-145, bla TEM-1, bla CTX-M-3, fosA6, oqxA5, oqxB26, and aac(3)-IId. This strain also exhibited high virulence characteristics such as biofilm formation and serum resistance.
Evaluation of the Xpert Carba-R assay for quantifying carbapenemase-producing bacterial load in stool samples.
The study evaluated the Xpert Carba-R assay for quantifying carbapenemase-producing bacterial load in stool samples, demonstrating the ability to estimate bacterial loads for bla NDM, bla KPC, and bla OXA-48 with acceptable accuracy, while bla IMP-1 and bla VIM showed higher limits of detection.
Plasmid-Mediated Spread of Carbapenem Resistance in Enterobacterales: A Three-Year Genome-Based Survey.
The study identified various carbapenemase genes, including KPC-2, KPC-3, OXA-48, NDM-1, NDM-5, VIM-1, OXA-23, and OXA-72, which contribute to carbapenem resistance in Enterobacterales. These genes were found in multiple species and were associated with plasmid-mediated resistance.
Plasmid-Mediated Spread of Carbapenem Resistance in Enterobacterales: A Three-Year Genome-Based Survey.
The study identified various carbapenemase genes, including KPC-2, KPC-3, OXA-48, NDM-1, NDM-5, VIM-1, OXA-23, and OXA-72, which contribute to carbapenem resistance in Enterobacterales. These genes were found in multiple species and were associated with plasmid-mediated resistance.
Molecular characteristics and pathogenic mechanisms of KPC-3 producing hypervirulent carbapenem-resistant Klebsiella pneumoniae (ST23-K1).
The study identified a single hypervirulent carbapenem-resistant Klebsiella pneumoniae strain, CR-hvKP57, which harbors the blaKPC-3 carbapenem resistance gene on a resistance plasmid. This strain exhibited high-level resistance to most antibiotics except polymyxin, tigecycline, and ceftazidime/avibactam.
Ginkgolic Acid as a carbapenem synergist against KPC-2 positive Klebsiella pneumoniae.
Ginkgolic Acid (GA) effectively inhibits KPC-2, a beta-lactamase responsible for carbapenem resistance in Klebsiella pneumoniae, and enhances the bactericidal effect of carbapenems by disrupting bacterial membranes.
In vitro Synergistic and Bactericidal Effects of Aztreonam in Combination with Ceftazidime/ Avibactam, Meropenem/Vaborbactam and Imipenem/Relebactam Against Dual-Carbapenemase-Producing Enterobacterales.
The study identified multiple carbapenemase genes, including bla KPC-2, bla NDM-1, bla NDM-5, bla IMP-26, and bla IMP-4, in dual-carbapenemase-producing Enterobacterales. These genes conferred resistance to carbapenems and other beta-lactam antibiotics. The combinations of aztreonam with ceftazidime/avibactam, meropenem/vaborbactam, and imipenem/relebactam showed synergistic and bactericidal effects against these strains.
Methodological Evaluation of Carbapenemase Detection by Different Methods.
The study evaluated different methods for detecting carbapenemases, identifying blaKPC-2 and NDM-1 as the main carbapenemase genes in the strains analyzed.
Evolution of ceftazidime-avibactam resistance driven by mutations in double-copy bla(KPC-2) to bla(KPC-189) during treatment of ST11 carbapenem-resistant Klebsiella pneumoniae.
The study identifies blaKPC-2 and blaKPC-189 as responsible for ceftazidime-avibactam resistance in carbapenem-resistant Klebsiella pneumoniae isolates, demonstrating their functionality through cloning and MIC testing.
Evolution of ceftazidime-avibactam resistance driven by mutations in double-copy bla(KPC-2) to bla(KPC-189) during treatment of ST11 carbapenem-resistant Klebsiella pneumoniae.
The study identifies blaKPC-2 and blaKPC-189 as responsible for ceftazidime-avibactam resistance in carbapenem-resistant Klebsiella pneumoniae isolates, demonstrating their functionality through cloning and MIC testing.
Evolution of ceftazidime-avibactam resistance driven by mutations in double-copy bla(KPC-2) to bla(KPC-189) during treatment of ST11 carbapenem-resistant Klebsiella pneumoniae.
Evolution of ceftazidime-avibactam resistance driven by mutations in double-copy bla(KPC-2) to bla(KPC-189) during treatment of ST11 carbapenem-resistant Klebsiella pneumoniae.
Geneticand phenotypic characterization of a novel ST45-K43 carbapenem-resistant Klebsiella pneumoniae strain causing bloodstream infection: a potential clinical threat.
The study identifies a novel ST45-K43 carbapenem-resistant Klebsiella pneumoniae strain, 18SHX166, carrying multiple antimicrobial resistance genes including bla KPC-2, oqxA, oqxB, fosA7, bla SHV26, aph(3′)-Ia, bleO, tet(A), dfrA14, and bla TEM1B. The strain exhibits multidrug resistance and high transmissibility.
Cefepime-taniborbactam activity against antimicrobial-resistant clinical isolates of Enterobacterales and Pseudomonas aeruginosa: GEARS global surveillance programme 2018-22.
Cefepime-taniborbactam showed potent in vitro activity against Enterobacterales and P. aeruginosa, particularly effective against isolates with carbapenemase genes such as blaIMP, blaNDM, and blaVIM, as well as those with mutations in ftsI, ompK35, and ompK36.
Evaluation of an expanded antibiotic resistance gene panel on prediction of antimicrobial susceptibility results for Gram-negative bacteria in blood cultures.
The study evaluated the QIAstat-Dx BCID panel for detecting antibiotic resistance genes in Gram-negative bacteria from blood cultures. It found that the panel effectively identified several resistance genes, including bla CTX-M, bla KPC, bla NDM, and others, which are crucial for predicting antimicrobial susceptibility. The panel showed high sensitivity and specificity for identifying resistance genes and predicting susceptibility, especially for beta-lactam antibiotics.
Molecular epidemiology and carbapenem resistance mechanisms of Pseudomonas aeruginosa isolated from a hospital in Fujian, China.
The study identifies several carbapenem resistance mechanisms in Pseudomonas aeruginosa, including carbapenemase genes (blaIMP, blaVIM, blaNDM, blaKPC), mutations in the outer membrane porins oprD and opdP, and overexpression of multidrug efflux pumps.
Molecular epidemiology and carbapenem resistance mechanisms of Pseudomonas aeruginosa isolated from a hospital in Fujian, China.
The study identifies several carbapenem resistance mechanisms in Pseudomonas aeruginosa, including carbapenemase genes (blaIMP, blaVIM, blaNDM, blaKPC), mutations in the outer membrane porins oprD and opdP, and overexpression of multidrug efflux pumps.
Detection of cefiderocol and aztreonam/avibactam resistance in epidemic Escherichia coli ST-361 carrying bla(NDM-5) and bla(KPC-3) from foreign fighters evacuated from Ukraine.
The study identifies bla(NDM-5), bla(KPC-3), and bla(CMY-145) as key contributors to carbapenem and cephalosporin resistance in E. coli ST-361 isolates, alongside a PBP3 YRIN insertion that enhances resistance to cefiderocol and aztreonam/avibactam.
Analysis of the Association Between Antimicrobial Resistance Genes and Virulence Factors in ST11 and Non-ST11 CR-KP Bloodstream Infections in the Intensive Care Unit.
The study identifies bla KPC-2 as the primary carbapenemase in ST11 CR-KP, with higher carriage rates compared to non-ST11 CR-KP. It also detects bla NDM-5 in non-ST11 CR-KP and bla OXA-23 in some strains. The study highlights the higher resistance rates of ST11 CR-KP to various antibiotics and its increased virulence gene carriage.
Antimicrobial susceptibility to last-resort antibiotics in carbapenemase-producing bacteria from Ukrainian patients.
The study identified multiple carbapenemase genes, including blaNDM, blaOXA-48, blaKPC, blaIMP, blaVIM, and blaGES, which confer resistance to last-resort antibiotics in carbapenemase-producing bacteria from Ukrainian patients.
Genetic landscape of ESBL producing international clone ST410 of Escherichia coli from pediatric infections in Shenzhen, China.
The study identifies multiple ESBL genes, including bla CTX-M, bla TEM, and bla SHV, along with other resistance genes such as bla OXA-1, bla KPC-2, bla NDM-1, and others, contributing to multidrug resistance in E. coli ST410 isolates from pediatric infections in Shenzhen, China.
Genetic landscape of ESBL producing international clone ST410 of Escherichia coli from pediatric infections in Shenzhen, China.
The study identifies multiple ESBL genes, including bla CTX-M, bla TEM, and bla SHV, along with other resistance genes such as bla OXA-1, bla KPC-2, bla NDM-1, and others, contributing to multidrug resistance in E. coli ST410 isolates from pediatric infections in Shenzhen, China.
Prevalence and molecular characterization of carbapenem-resistant Enterobacterales in patients from a public referral hospital in a non-metropolitan region of Brazil during and post the SARS-CoV-2 pandemic.
The study identified the bla KPC and bla NDM genes as the primary carbapenem resistance genes in carbapenem-resistant Enterobacterales (CRE) isolates from a Brazilian hospital, with bla KPC being the most prevalent.
Metabolic Reprogramming of Klebsiella pneumoniae Exposed to Serum and Its Potential Implications in Host Immune System Evasion and Resistance.
The study identifies the blaKPC gene as a determinant of carbapenem resistance in Klebsiella pneumoniae ACH2, highlighting its role in serum resistance and metabolic reprogramming.
Whole-genome sequencing of Klebsiella pneumoniae MDR circulating in a pediatric hospital setting: a comprehensive genome analysis of isolates from Guayaquil, Ecuador.
The study identified several AMR genes and mutations in K. pneumoniae isolates from Ecuador, including bla KPC-3, bla OXA-9, aadA1, aac(6')-Ib-AKT, and mutations in ompK35, ompK36, ompK37, gyrA, parC, and acrR, contributing to resistance against beta-lactams, aminoglycosides, fluoroquinolones, and other antibiotics.
Whole-genome sequencing of Klebsiella pneumoniae MDR circulating in a pediatric hospital setting: a comprehensive genome analysis of isolates from Guayaquil, Ecuador.
The study identified several AMR genes and mutations in K. pneumoniae isolates from Ecuador, including bla KPC-3, bla OXA-9, aadA1, aac(6')-Ib-AKT, and mutations in ompK35, ompK36, ompK37, gyrA, parC, and acrR, contributing to resistance against beta-lactams, aminoglycosides, fluoroquinolones, and other antibiotics.
First report of carbapenems encoding multidrug-resistant gram-negative bacteria from a pediatric hospital in Gaza Strip, Palestine.
The study identified multiple carbapenem resistance genes, including bla KPC-2, bla PDC-36/12, and bla POM-1, in multidrug-resistant Gram-negative bacteria isolated from a pediatric hospital in the Gaza Strip. These genes were found in various bacterial species such as Escherichia coli and Klebsiella pneumoniae.
In vivo development of resistance to novel β-lactam/β-lactamase inhibitor combinations in KPC-producing Klebsiella pneumoniae infections: a case series.
The study identified specific mutations in the blaKPC carbapenemase gene (D179Y mutation [KPC-33] and deletion Δ242-GT-243 [KPC-14]) associated with resistance to ceftazidime/avibactam, and porin loss (truncated OmpK35 and OmpK36 porins) linked to resistance to meropenem/vaborbactam and imipenem/cilastatin/relebactam in KPC-producing Klebsiella pneumoniae.
In vivo development of resistance to novel β-lactam/β-lactamase inhibitor combinations in KPC-producing Klebsiella pneumoniae infections: a case series.
The study identified specific mutations in the blaKPC carbapenemase gene (D179Y mutation [KPC-33] and deletion Δ242-GT-243 [KPC-14]) associated with resistance to ceftazidime/avibactam, and porin loss (truncated OmpK35 and OmpK36 porins) linked to resistance to meropenem/vaborbactam and imipenem/cilastatin/relebactam in KPC-producing Klebsiella pneumoniae.
Clinical and genomic characterization of carbapenem-resistant Enterobacterales bloodstream infections in patients with hematologic malignancies.
The study identified bla KPC-2 and bla NDM-5 as the主要 carbapenemase genes in carbapenem-resistant Enterobacterales (CRE) bloodstream infections among patients with hematologic malignancies.
Blood-rsCDM: a new rapid and simplified carbapenemase detection method for detecting carbapenemases in Enterobacterales directly from positive blood cultures.
The study presents a new rapid and simplified method, Blood-rsCDM, for detecting and characterizing carbapenemases in Enterobacterales directly from positive blood cultures. It effectively identifies various carbapenemase types, including KPC, NDM, IMP, VIM, and OXA-181, with high sensitivity and specificity.
Development and Validation of a Point-of-Care Platform for Rapid Detection of ESBL and Carbapenemase Genes Using URECA-LAMP
The study presents a novel machine-learning-aided platform called URECA-LAMP for the rapid detection of ESBLs and carbapenemases in clinical isolates and urine samples. The platform uses LAMP technology combined with a smartphone application for automated interpretation of results, achieving high agreement rates with WGS results.
Prevalence, Characterization, and Epidemiological Relationships between ESBL and Carbapenemase-Producing Escherichia coli, Klebsiella pneumoniae, and Acinetobacter spp. Isolated from Humans and the Kitchen Environment of Two Greek Hospitals.
The study identified various beta-lactamase genes, including bla SHV, bla CTX-M, bla TEM, bla KPC, bla NDM, bla OXA-48, bla OXA-23, and bla OXA-51, in ESBL and carbapenemase-producing E. coli, K. pneumoniae, and Acinetobacter spp. isolates from hospital kitchens and staff.
High prevalence of carbapenem-resistant Enterobacter cloacae complex in a tertiary hospital over a decade.
The study identified blaNDM-1, blaNDM-5, blaIMP-4, and blaKPC-2 as the main carbapenem resistance genes in carbapenem-resistant Enterobacter cloacae complex (CRECC) isolates. Additionally, various extended-spectrum beta-lactamase (ESBL) and AmpC beta-lactamase genes were detected, contributing to multidrug resistance.
Carbapenem-resistant Klebsiella oxytoca transmission linked to preoperative shaving in emergency neurosurgery, tracked by rapid detection via chromogenic medium and whole genome sequencing.
The study identified carbapenem-resistant Klebsiella oxytoca (CRKO) isolates carrying bla KPC-2 and bla OXY-5 resistance genes, which conferred resistance to multiple carbapenems and other beta-lactam antibiotics. Contaminated razors were found to be a source of CRKO transmission in emergency neurosurgery.
Longitudinal genomic surveillance of a UK intensive care unit shows a lack of patient colonisation by multi-drug-resistant Gram-negative bacterial pathogens.
The study found a lack of multi-drug resistance in the QEHB ICU, with only one carbapenemase-producing organism, a Citrobacter carrying bla KPC-2, isolated. No evidence of nosocomial acquisition or circulation of colonising E. coli was found.
Myroides species, pathogenic spectrum and clinical microbiology sight in Mexican isolates.
The study identified multiple AMR genes in Myroides spp. isolates, including beta-lactamases (blaIMP-27, blaIMP-35, blaGOB-16, blaMUS-1, blaOXA-229, blaOXA-351, blaOXA-97), erythromycin esterase (ereB), and polymyxin resistance genes (mcr-3.6, mcr-3.7, mcr-3.10), indicating a high level of multidrug resistance.
Detection of carbapenemases in Enterobacterales and other Gram-negative bacilli recovered from hospital and municipal wastewater in Mexico City.
The study identified several carbapenemase genes, including bla KPC, bla NDM, bla VIM−2, bla OXA−48, bla NDM−1, and bla NDM−5, in various Gram-negative bacteria from hospital and municipal wastewater in Mexico City.
The characterization of an IncN-IncR fusion plasmid co-harboring bla(TEM-40), bla(KPC-2), and bla(IMP-4) derived from ST1393 Klebsiella pneumoniae.
The study characterizes a novel IncN-IncR fusion plasmid carrying bla(KPC-2), bla(IMP-4), and bla(TEM-40) genes, along with qnrS1, in a ST1393 Klebsiella pneumoniae strain. The plasmid was found to confer resistance to multiple carbapenems and other antibiotics.
First documentation of a clinical multidrug-resistant Enterobacter chuandaensis ST2493 isolate co-harboring bla(NDM-1) and two bla(KPC-2) bearing plasmids.
The study reports the first clinical multidrug-resistant Enterobacter chuandaensis ST2493 isolate co-harboring bla(NDM-1) and two bla(KPC-2) bearing plasmids, highlighting the potential for the dissemination of carbapenemase-encoding genes in novel species.
Public health concern of antimicrobial resistance and virulence determinants in E. coli isolates from oysters in Egypt.
The study identified multiple AMR genes in E. coli isolates from oysters in Egypt, including bla TEM, bla CTX-M, bla SHV, bla OXA-1, bla CMY-2, bla KPC, bla NDM, bla OXA-48, and bla VIM, as well as virulence genes such as papC, sfa, exhA, eaeA, and estA.
Ceftazidime-avibactam treatment dilemma of bla(KPC-2)-containing Klebsiella pneumoniae due to the development of co-existence of mixed strains carrying bla(KPC-2) or bla(KPC-33) in lung transplant recipients.
The study identifies the co-existence of mixed strains carrying bla(KPC-2) or bla(KPC-33) in lung transplant recipients treated with ceftazidime-avibactam, demonstrating the dynamic shift in resistance phenotypes due to the emergence of bla(KPC-33) from bla(KPC-2).
Ceftazidime-avibactam treatment dilemma of bla(KPC-2)-containing Klebsiella pneumoniae due to the development of co-existence of mixed strains carrying bla(KPC-2) or bla(KPC-33) in lung transplant recipients.
The study identifies the co-existence of mixed strains carrying bla(KPC-2) or bla(KPC-33) in lung transplant recipients treated with ceftazidime-avibactam, demonstrating the dynamic shift in resistance phenotypes due to the emergence of bla(KPC-33) from bla(KPC-2).
Emergence of hypervirulent and carbapenem-resistant Klebsiella pneumoniae from 2014 - 2021 in Central and Eastern China: a molecular, biological, and epidemiological study.
The study identified blaKPC-2, blaNDM-5, blaOXA-232, qnrS, and fosA as the primary resistance genes in hypervirulent and carbapenem-resistant Klebsiella pneumoniae strains, highlighting their role in multidrug resistance.
Prevalence of bla(OXA-48) and other carbapenemase encoding genes among carbapenem-resistant Pseudomonas aeruginosa clinical isolates in Egypt.
The study identified blaVIM, blaOXA-48, blaKPC, blaIMP, blaGES, and blaNDM as prevalent carbapenemase encoding genes among carbapenem-resistant P. aeruginosa isolates in Egypt. blaOXA-48 was frequently associated with the Tn1999 transposon.
Limited Evidence of Spillover of Antimicrobial-Resistant Klebsiella pneumoniae from Animal/Environmental Reservoirs to Humans in Vellore, India.
The study found that clinical and hospital sewage isolates of Klebsiella pneumoniae had a higher number of AMR genes compared to livestock isolates, suggesting that AMR is more prevalent in human-associated settings. Several beta-lactamase genes, including blaCTX-M, blaNDM, blaKPC, and blaOXA-48, were detected in clinical isolates, contributing to resistance against carbapenems and other antibiotics. Other AMR genes such as qnrS1, aac(6')-Ib, aadA, ermB, mefA, tet(A), mph(A), cat, cfr, vanA, mcr-1, fosA, sul1, and dfrA1 were also identified, highlighting the diversity of resistance mechanisms in K. pneumoniae.
A nosocomial outbreak of colistin and carbapenem-resistant hypervirulent Klebsiella pneumoniae in a large teaching hospital.
Carbapenemase-producing bacteria recovered from Nairobi River, Kenya surface water and from nearby anthropogenic and zoonotic sources.
The study identified multiple carbapenemase-encoding genes, including bla NDM, bla KPC, bla VIM, bla OXA-48-like, bla IMP, and bla GES, in various bacterial species from Nairobi River and surrounding environments.
Mutant prevention concentrations, in vitro resistance evolution dynamics, and mechanisms of resistance to imipenem and imipenem/relebactam in carbapenem-susceptible Klebsiella pneumoniae isolates showing ceftazidime/avibactam resistance.
The study identifies several KPC variants, including KPC-31, KPC-3, KPC-3 D179N, KPC-35, KPC-2, KPC-2 L169A, and KPC-2 L169T, which contribute to resistance to ceftazidime/avibactam. Additionally, mutations in the outer membrane porin OmpK36 are associated with reduced internalization of carbapenems, contributing to resistance.
Mutant prevention concentrations, in vitro resistance evolution dynamics, and mechanisms of resistance to imipenem and imipenem/relebactam in carbapenem-susceptible Klebsiella pneumoniae isolates showing ceftazidime/avibactam resistance.
The study identifies several KPC variants, including KPC-31, KPC-3, KPC-3 D179N, KPC-35, KPC-2, KPC-2 L169A, and KPC-2 L169T, which contribute to resistance to ceftazidime/avibactam. Additionally, mutations in the outer membrane porin OmpK36 are associated with reduced internalization of carbapenems, contributing to resistance.
Mutant prevention concentrations, in vitro resistance evolution dynamics, and mechanisms of resistance to imipenem and imipenem/relebactam in carbapenem-susceptible Klebsiella pneumoniae isolates showing ceftazidime/avibactam resistance.
The study identifies several KPC variants, including KPC-31, KPC-3, KPC-3 D179N, KPC-35, KPC-2, KPC-2 L169A, and KPC-2 L169T, which contribute to resistance to ceftazidime/avibactam. Additionally, mutations in the outer membrane porin OmpK36 are associated with reduced internalization of carbapenems, contributing to resistance.
Mutant prevention concentrations, in vitro resistance evolution dynamics, and mechanisms of resistance to imipenem and imipenem/relebactam in carbapenem-susceptible Klebsiella pneumoniae isolates showing ceftazidime/avibactam resistance.
The study identifies several KPC variants, including KPC-31, KPC-3, KPC-3 D179N, KPC-35, KPC-2, KPC-2 L169A, and KPC-2 L169T, which contribute to resistance to ceftazidime/avibactam. Additionally, mutations in the outer membrane porin OmpK36 are associated with reduced internalization of carbapenems, contributing to resistance.
Mutant prevention concentrations, in vitro resistance evolution dynamics, and mechanisms of resistance to imipenem and imipenem/relebactam in carbapenem-susceptible Klebsiella pneumoniae isolates showing ceftazidime/avibactam resistance.
The study identifies several KPC variants, including KPC-31, KPC-3, KPC-3 D179N, KPC-35, KPC-2, KPC-2 L169A, and KPC-2 L169T, which contribute to resistance to ceftazidime/avibactam. Additionally, mutations in the outer membrane porin OmpK36 are associated with reduced internalization of carbapenems, contributing to resistance.
Mutant prevention concentrations, in vitro resistance evolution dynamics, and mechanisms of resistance to imipenem and imipenem/relebactam in carbapenem-susceptible Klebsiella pneumoniae isolates showing ceftazidime/avibactam resistance.
The study identifies several KPC variants, including KPC-31, KPC-3, KPC-3 D179N, KPC-35, KPC-2, KPC-2 L169A, and KPC-2 L169T, which contribute to resistance to ceftazidime/avibactam. Additionally, mutations in the outer membrane porin OmpK36 are associated with reduced internalization of carbapenems, contributing to resistance.
Mutant prevention concentrations, in vitro resistance evolution dynamics, and mechanisms of resistance to imipenem and imipenem/relebactam in carbapenem-susceptible Klebsiella pneumoniae isolates showing ceftazidime/avibactam resistance.
The study identifies several KPC variants, including KPC-31, KPC-3, KPC-3 D179N, KPC-35, KPC-2, KPC-2 L169A, and KPC-2 L169T, which contribute to resistance to ceftazidime/avibactam. Additionally, mutations in the outer membrane porin OmpK36 are associated with reduced internalization of carbapenems, contributing to resistance.
Molecular characteristics and antibiotic resistance mechanisms of multidrug-resistant Pseudomonas aeruginosa in Nanning, China.
The study identified NDM-1, IMP-9, VIM-2, and KPC-2 carbapenemase genes as major contributors to multidrug resistance in Pseudomonas aeruginosa strains in Nanning, China.
Phenotypic and genomic characterization of ST11-K1 CR-hvKP with highly homologous bla(KPC-2)-bearing plasmids in China.
Seven ST11-K1 CR-hvKP isolates were found to carry highly homologous blaKPC-2-bearing plasmids, demonstrating multidrug resistance and hypervirulence. Key resistance genes included blaKPC-2, blaSHV-11, blaSHV-12, blaCTX-M-65, blaTEM-1, rmtB, oqxB, tetA/B, sul4, mcr-8, fosA, and mupB.
Antimicrobial Resistance in Acinetobacter baumannii and Carbapenem-Resistant Enterobacteriaceae
The paper discusses the mechanisms of carbapenem resistance in various bacterial species, highlighting the roles of beta-lactamases such as KPC, NDM, VIM, IMP, and OXA-48. These genes are plasmid-encoded and facilitate horizontal gene transfer, contributing to multidrug resistance.
Development of a Recombinase Polymerase Amplification-Coupled CRISPR/Cas12a Platform for Rapid Detection of Antimicrobial-Resistant Genes in Carbapenem-Resistant Enterobacterales.
The study developed a rapid and accurate RPA-CRISPR/Cas12a platform (RCCS) for detecting carbapenemase genes blaKPC and blaNDM in carbapenem-resistant Enterobacterales, achieving high sensitivity and specificity within 50 minutes.
Multidrug-resistant ESBL-producing Klebsiella pneumoniae complex in Czech hospitals, wastewaters and surface waters.
The study identified multidrug-resistant Klebsiella pneumoniae complex isolates carrying various beta-lactamase genes such as bla CTX-M-15, bla GES-51, bla OXA-48, bla NDM-1, and bla KPC-3, along with other resistance genes like oqxA, oqxB, sul2, aph(6)-Id, dfr14, qnrB1, and aac(6')-Ib-cr.
A Novel Variant of KPC-179 Conferring Ceftazidime-Avibactam Resistance in a Carbapenem-Resistant Klebsiella pneumoniae Isolate.
Genomic characterization of a bla (KPC-2)-producing IncM2 plasmid harboring transposon ΔTn6296 in Klebsiella michiganensis.
The study characterizes a bla KPC-2 -producing IncM2 plasmid harboring transposon ΔTn6296 in Klebsiella michiganensis, highlighting the role of this plasmid in the dissemination of carbapenem resistance.
Evaluation of DNA extraction kits for long-read shotgun metagenomics using Oxford Nanopore sequencing for rapid taxonomic and antimicrobial resistance detection.
The study evaluated DNA extraction kits for long-read metagenomics and identified several AMR genes, including ctx-m-65, kpc-2, oxa-66, pdc-167, oxa-486, vanA, and mecA, in the ESKAPE Mock community using the QIAamp PowerFecal Pro DNA kit.
Clinical evaluation of advanced MALDI-TOF MS for carbapenemase subtyping in Gram-negative isolates.
The study evaluates an advanced MALDI-TOF MS method (A-MALDI) for the accurate identification and subtyping of carbapenemases in Gram-negative clinical isolates, demonstrating 100% accuracy and precision in detecting various carbapenemase subtypes including KPC, NDM, OXA, and GES.
Two outbreak cases involving ST65-KL2 and ST11-KL64 hypervirulent carbapenem-resistant Klebsiella pneumoniae: similarity and diversity analysis.
The study identifies two outbreak cases of hypervirulent carbapenem-resistant Klebsiella pneumoniae (hvCRKP) strains, ST65-KL2 and ST11-KL64, highlighting their distinct resistance mechanisms and virulence profiles.
Rapid detection of carbapenemase production in Aeromonas using phenotypic tests based on colorimetric microtube assay.
The study evaluates phenotypic tests for rapid detection of carbapenemase production in Aeromonas, focusing on genes such as bla CphA, bla KPC, and bla NDM.
Multispecies emergence of dual bla(KPC/NDM) carbapenemase-producing Enterobacterales recovered from invasive infections in Chile.
The study reports the emergence of multiple species of dual carbapenemase-producing Enterobacterales (CP-CRE) in Chile, which simultaneously harbor bla KPC and bla NDM genes, leading to resistance against most β-lactam antibiotics except cefiderocol.
Adaptive evolution of extensive drug resistance and persistence in epidemic ST11 KPC-producing Klebsiella pneumoniae during antimicrobial chemotherapy.
The study identifies the integration of IS Kpn26 into the mgrB gene, leading to mgrB inactivation and colistin resistance, as well as novel bla KPC-2 variants (bla KPC-71 and bla KPC-179 ) that confer resistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
Adaptive evolution of extensive drug resistance and persistence in epidemic ST11 KPC-producing Klebsiella pneumoniae during antimicrobial chemotherapy.
The study identifies the integration of IS Kpn26 into the mgrB gene, leading to mgrB inactivation and colistin resistance, as well as novel bla KPC-2 variants (bla KPC-71 and bla KPC-179 ) that confer resistance to ceftazidime/avibactam in KPC-producing Klebsiella pneumoniae.
Adaptive evolution of extensive drug resistance and persistence in epidemic ST11 KPC-producing Klebsiella pneumoniae during antimicrobial chemotherapy.
Adaptive evolution of extensive drug resistance and persistence in epidemic ST11 KPC-producing Klebsiella pneumoniae during antimicrobial chemotherapy.
The association between the genetic structures of commonly incompatible plasmids in Gram-negative bacteria, their distribution and the resistance genes.
The study characterizes various resistance genes carried by incompatible plasmids in Gram-negative bacteria, highlighting their role in the spread of antibiotic resistance. Key genes include beta-lactamases like bla VIM-1, bla SHV-12, bla TEM-1B, and bla CTX-M-15, as well as sulfonamide resistance genes sul1 and sul2, tetracycline resistance gene tetA, and polymyxin resistance gene mcr-1.
The association between the genetic structures of commonly incompatible plasmids in Gram-negative bacteria, their distribution and the resistance genes.
The study characterizes various resistance genes carried by incompatible plasmids in Gram-negative bacteria, highlighting their role in the spread of antibiotic resistance. Key genes include beta-lactamases like bla VIM-1, bla SHV-12, bla TEM-1B, and bla CTX-M-15, as well as sulfonamide resistance genes sul1 and sul2, tetracycline resistance gene tetA, and polymyxin resistance gene mcr-1.
Frequencies and mechanisms of mutational resistance to ceftibuten/avibactam in Enterobacterales.
Frequencies and mechanisms of mutational resistance to ceftibuten/avibactam in Enterobacterales.
Frequencies and mechanisms of mutational resistance to ceftibuten/avibactam in Enterobacterales.
Molecular Epidemiology and Clinical Characterization of Carbapenemase-Producing Enterobacter Species From an International Cohort.
The study identifies bla KPC, bla NDM, bla IMP, bla IMI, and bla VIM as the primary carbapenemase genes in carbapenemase-producing Enterobacter species, along with fluoroquinolone resistance mutations in gyrA and parC, and porin gene mutations in ompF and ompC.
Activity of ceftolozane/tazobactam and comparators against gram-negative bacilli: Results from the Study for Monitoring Antimicrobial Resistance Trends (SMART - Brazil), 2018‒2021.
The study identified several beta-lactamase genes, including bla KPC-2, bla CTX-M variants, and bla NDM-1, which confer resistance to carbapenems and cephalosporins in Gram-negative bacilli in Brazil. These genes were detected in Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolates.
Activity of ceftolozane/tazobactam and comparators against gram-negative bacilli: Results from the Study for Monitoring Antimicrobial Resistance Trends (SMART - Brazil), 2018‒2021.
The study identified several beta-lactamase genes, including bla KPC-2, bla CTX-M variants, and bla NDM-1, which confer resistance to carbapenems and cephalosporins in Gram-negative bacilli in Brazil. These genes were detected in Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolates.
Activity of ceftolozane/tazobactam and comparators against gram-negative bacilli: Results from the Study for Monitoring Antimicrobial Resistance Trends (SMART - Brazil), 2018‒2021.
The study identified several beta-lactamase genes, including bla KPC-2, bla CTX-M variants, and bla NDM-1, which confer resistance to carbapenems and cephalosporins in Gram-negative bacilli in Brazil. These genes were detected in Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolates.
Activity of ceftolozane/tazobactam and comparators against gram-negative bacilli: Results from the Study for Monitoring Antimicrobial Resistance Trends (SMART - Brazil), 2018‒2021.
The study identified several beta-lactamase genes, including bla KPC-2, bla CTX-M variants, and bla NDM-1, which confer resistance to carbapenems and cephalosporins in Gram-negative bacilli in Brazil. These genes were detected in Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolates.
Emergent Escherichia coli of the highly virulent B2-ST1193 clone producing KPC-2 carbapenemase in ready-to-eat vegetables.
The study identifies the KPC-2 carbapenemase-producing E. coli ST1193 clone in ready-to-eat vegetables, highlighting its multidrug resistance and acid tolerance mechanisms.
A single-center retrospective study of the molecular epidemiological characteristics of different Klebsiella pneumoniae infections in northern China.
The study identified multidrug-resistant ST11 and hypervirulent ST23 as prevalent Klebsiella pneumoniae strains in northern China, highlighting the co-harborance of resistance genes such as rmtB, TEM-1, and KPC-2 in ST11 strains.
Antibacterial and Anti-biofilm Effects of Thymoquinone Against Carbapenem-Resistant Uropathogenic Escherichia coli.
Thymoquinone inhibits the expression of the carbapenemase gene bla KPC and efflux pump genes in carbapenem-resistant uropathogenic Escherichia coli, reducing motility and biofilm formation.
Effect of Meropenem on Conjugative Plasmid Transfer in Klebsiella pneumoniae.
The study identifies bla KPC and bla OXA-48 carbapenemase genes as critical in mediating resistance to meropenem in Klebsiella pneumoniae, highlighting their role in plasmid-mediated resistance and the impact of meropenem concentrations on conjugative plasmid transfer.
Epidemiological and genetic characteristics of clinical carbapenemase-producing Enterobacterales isolates from Batna hospitals in Algeria.
The study identified bla OXA−48−like, bla NDM, bla VIM, and bla KPC genes as the primary carbapenemase genes in carbapenem-resistant Enterobacterales isolates from Batna hospitals in Algeria. It also reported novel combinations of these genes, highlighting the complexity of resistance mechanisms.
In vitro activity and genomic characterization of KPC-producing Klebsiella pneumoniae clinical blood culture isolates resistant to ceftazidime/avibactam, meropenem/vaborbactam, imipenem/relebactam: an Italian nationwide multicentre observational study (2022-23).
In vitro activity and genomic characterization of KPC-producing Klebsiella pneumoniae clinical blood culture isolates resistant to ceftazidime/avibactam, meropenem/vaborbactam, imipenem/relebactam: an Italian nationwide multicentre observational study (2022-23).
In vitro activity and genomic characterization of KPC-producing Klebsiella pneumoniae clinical blood culture isolates resistant to ceftazidime/avibactam, meropenem/vaborbactam, imipenem/relebactam: an Italian nationwide multicentre observational study (2022-23).
In vitro activity and genomic characterization of KPC-producing Klebsiella pneumoniae clinical blood culture isolates resistant to ceftazidime/avibactam, meropenem/vaborbactam, imipenem/relebactam: an Italian nationwide multicentre observational study (2022-23).
Emergence and clonal dissemination of KPC-2- and NDM-1-coharboring Citrobacter freundii in China with an IncR plasmid.
The study identifies the presence of blaKPC-2 and blaNDM-1 on an IncR plasmid in eight KPC-2-NDM-1-Citrobacter freundii strains, highlighting their carbapenem resistance and clonal dissemination in a children's hospital in China.
Evaluation of the effect of BioFire FilmArray nested multiplex polymerase chain reaction method on rapid pathogen identification and antimicrobial stewardship in sepsis.
The study evaluated the BioFire FilmArray Blood Culture Identification 2 panel for rapid pathogen identification and antimicrobial resistance detection in sepsis patients, demonstrating high concordance with conventional methods and improved turnaround time for antimicrobial stewardship.
Decadal Evolution of KPC-related plasmids in Pseudomonas aeruginosa high-risk clone ST463 in Zhejiang, China.
The study identifies blaKPC-2 as a key gene responsible for carbapenem resistance in Pseudomonas aeruginosa ST463 clone, highlighting the evolutionary advantages of type I plasmids with fragment deletions.
Characterization of a novel KPC-2 variant, KPC-228, conferring resistance to ceftazidime-avibactam in an ST11-KL64 hypervirulent Klebsiella pneumoniae.
Overexpression of KPC contributes to ceftazidime-avibactam heteroresistance in clinical isolates of carbapenem-resistant Klebsiella pneumoniae.
The study identifies that overexpression of bla KPC contributes to ceftazidime-avibactam heteroresistance in carbapenem-resistant Klebsiella pneumoniae isolates.
Identification of beta-lactamase genes and molecular genotyping of multidrug-resistant clinical isolates of Klebsiella pneumoniae.
The study identified the presence of beta-lactamase genes bla NDM, bla KPC, bla TEM, bla SHV, and bla CTX−M in multidrug-resistant K. pneumoniae isolates, highlighting the high prevalence of bla CTX−M and the significance of these genes in antibiotic resistance.
Emergence and clinical challenges of ST11-K64 carbapenem-resistant Klebsiella pneumoniae: molecular insights and implications for antimicrobial resistance and virulence in Southwest China.
The study identifies blaKPC-2 as the predominant carbapenemase gene in ST11-K64 CRKP, along with virulence genes rmpA, rmpA2, iucA, and iutA, highlighting the strain's high antimicrobial resistance and virulence.
National Multicenter Study on the Prevalence of Carbapenemase-Producing Enterobacteriaceae in the Post-COVID-19 Era in Argentina: The RECAPT-AR Study.
The study identified bla NDM, bla KPC, and bla OXA-163 as the main carbapenemase genes in Enterobacterales isolates from Argentina, with NDM and KPC being the most prevalent.
National Cohort of Compassionate Use of Meropenem-Vaborbactam: No Benefit over Meropenem for Pseudomonas aeruginosa.
The study found that vaborbactam did not provide any advantage in treating P. aeruginosa infections, as the addition of vaborbactam to meropenem did not modify the MIC. However, MEM-VAB was effective against KPC-3-producing K. pneumoniae and B. multivorans, significantly reducing the MIC of meropenem.
Emerging carbapenem-resistant Klebsiella pneumoniae in a tertiary care hospital in Lima, Peru.
The study identifies blaNDM-1, blaKPC-2, and blaIMP-74 as the primary carbapenem resistance genes in CRKP isolates from Lima, Peru. Additionally, aac(6')-Ib and sul1 were found to confer resistance to aminoglycosides and sulfonamides, respectively.
ESKAPE pathogens rapidly develop resistance against antibiotics in development in vitro.
The study identifies that ESKAPE pathogens rapidly develop resistance against antibiotics in development in vitro, with resistance mutations already present in natural populations and mobile resistance genes prevalent in clinical isolates, soil, and human gut microbiomes.
Comparative analysis of salivary antimicrobial resistance genes in dental students: A PCR and questionnaire study.
The study identified several antimicrobial resistance (AMR) genes in the saliva of dental students, including blaCTX-M grp 1, blaCTX-M grp 9, blaCTX-M grp 8, blaOXA-48, blaKPC-1, blaVIM, DHA, ACC, MOX, armA, and rmtB. These genes are associated with resistance to beta-lactams, carbapenems, and aminoglycosides.
Multidrug-resistant ST11-KL64 hypervirulent Klebsiella pneumoniae with multiple bla- genes isolated from children's blood.
Global phylogeography and genetic characterization of carbapenem and ceftazidime-avibactam resistant KPC-33-producing Pseudomonas aeruginosa.
The study identifies blaKPC-33 as a gene responsible for ceftazidime-avibactam resistance in Pseudomonas aeruginosa, highlighting its role in the emergence of resistance under drug pressure.
Carbapenem-resistant Enterobacterales infection and colonization in patients with severe burns: a retrospective cohort study in a single burn center.
The study identified KPC and NDM carbapenemase-producing CRE strains in patients with severe burns, highlighting the prevalence of carbapenem resistance in this population.
Intricate interplay of CRISPR-Cas systems, anti-CRISPR proteins, and antimicrobial resistance genes in a globally successful multi-drug resistant Klebsiella pneumoniae clone.
The study identifies the intricate interplay between CRISPR-Cas systems, anti-CRISPR proteins, and antimicrobial resistance genes in a globally successful multidrug-resistant Klebsiella pneumoniae clone, highlighting the prevalence of carbapenemase genes such as blaNDM, blaOXA-48-like, blaKPC, and extended-spectrum beta-lactamase CTX-M-15.
Intricate interplay of CRISPR-Cas systems, anti-CRISPR proteins, and antimicrobial resistance genes in a globally successful multi-drug resistant Klebsiella pneumoniae clone.
The study identifies the intricate interplay between CRISPR-Cas systems, anti-CRISPR proteins, and antimicrobial resistance genes in a globally successful multidrug-resistant Klebsiella pneumoniae clone, highlighting the prevalence of carbapenemase genes such as blaNDM, blaOXA-48-like, blaKPC, and extended-spectrum beta-lactamase CTX-M-15.
Clinical evaluation of a multiplex droplet digital PCR for diagnosing suspected bloodstream infections: a prospective study.
The study evaluated a multiplex droplet digital PCR (ddPCR) assay for diagnosing bloodstream infections, identifying key AMR genes such as bla KPC, bla NDM, bla VIM, bla IMP, bla OXA48, vanA, vanM, and mecA in clinical isolates.
Capture of mobile genetic elements following intercellular conjugation promotes the production of ST11-KL64 CR-hvKP.
The study characterizes the AMR genes and mutations involved in the conjugation process between hvKP and CRKP strains, highlighting the role of mobile genetic elements in the emergence of ST11-KL64 CR-hvKP strains.
Double carbapenemases in Klebsiella pneumoniae blood isolates: dissemination in a single medical center via multiple plasmids and a variety of highly efficient clones.
The study identifies bla KPC-2, bla KPC-3, and bla VIM-1 genes as the primary carbapenemase genes responsible for resistance in Klebsiella pneumoniae blood isolates. These genes were found on separate plasmids and contributed to extensive drug resistance.
Double carbapenemases in Klebsiella pneumoniae blood isolates: dissemination in a single medical center via multiple plasmids and a variety of highly efficient clones.
The study identifies bla KPC-2, bla KPC-3, and bla VIM-1 genes as the primary carbapenemase genes responsible for resistance in Klebsiella pneumoniae blood isolates. These genes were found on separate plasmids and contributed to extensive drug resistance.
Prevalence of β-lactam antibiotic resistance of Escherichia coli isolated from a neonatal intensive care unit.
The study identified several β-lactam resistance genes, including bla KPC-2, bla CTX-M-64, bla CTX-M-65, bla TEM-1, bla OXA-1, and bla DHA-1, in Escherichia coli isolates from a neonatal intensive care unit. These genes were found to confer resistance to various β-lactam antibiotics.
Comparative analysis of selected methods of carbapenemase determination among clinical Klebsiella pneumoniae.
The study evaluated methods for detecting carbapenemase-producing Klebsiella pneumoniae strains, identifying NDM, KPC, OXA-48, and VIM carbapenemases as the most prevalent.
Cefepime-taniborbactam and ceftibuten-ledaborbactam maintain activity against KPC variants that lead to ceftazidime-avibactam resistance.
Cefepime-taniborbactam (FEP-TAN) and ceftibuten-ledaborbactam (CTB-LED) maintain activity against KPC variants that lead to ceftazidime-avibactam (CAZ-AVI) resistance. Mutations in KPC-2 and KPC-3, including D179Y, V240G, and T243M, reduce the effectiveness of CAZ-AVI but not FEP-TAN or CTB-LED.
Cefepime-taniborbactam and ceftibuten-ledaborbactam maintain activity against KPC variants that lead to ceftazidime-avibactam resistance.
Cefepime-taniborbactam (FEP-TAN) and ceftibuten-ledaborbactam (CTB-LED) maintain activity against KPC variants that lead to ceftazidime-avibactam (CAZ-AVI) resistance. Mutations in KPC-2 and KPC-3, including D179Y, V240G, and T243M, reduce the effectiveness of CAZ-AVI but not FEP-TAN or CTB-LED.
Dissemination of bla(NDM)-harboring plasmids in carbapenem-resistant and hypervirulent Klebsiella pneumoniae.
The study identifies bla KPC-2, bla NDM-5, and bla NDM-1 as key carbapenemase genes in carbapenem-resistant Klebsiella pneumoniae (CRKP) strains, highlighting their role in resistance to carbapenems and cephalosporins.
Rapid Simultaneous Detection of the Clinically Relevant Carbapenemase Resistance Genes blaKPC, blaOXA48, blaVIM and blaNDM with the Newly Developed Ready-to-Use qPCR CarbaScan LyoBead.
The study introduces the qPCR CarbaScan LyoBead assay, a robust and efficient tool for detecting clinically relevant carbapenemase resistance genes, including blaKPC, blaOXA48, blaVIM, and blaNDM. The assay demonstrated 100% specificity and sensitivity across a diverse range of bacterial strains.
Small Molecules from Medicinal Plant Iris tectorum as Histidine Kinase Inhibitor to Resensitize β-Lactam-Resistant Escherichia coli.
Iris tectorum extract resensitized β-lactam-resistant E. coli by downregulating ESBL gene expression and reducing histidine kinase phosphorylation levels.
Comparison of the in vitro activities and resistance mechanisms against imipenem-relebactam and ceftazidime-avibactam in clinical KPC-producing Klebsiella pneumoniae isolated in China.
The study identified KPC mutations as a key mechanism of resistance to ceftazidime-avibactam (CAZ-AVI) in KPC-producing Klebsiella pneumoniae, while OmpK36 mutations were associated with resistance to imipenem-relebactam (IMI-REL).
Genomic characteristics and phylogenetic analyses of colonization and infection with carbapenem-resistant Klebsiella pneumoniae in multicenter intensive care units: a cohort study.
The study identified blaKPC-2 and blaNDM-1 as the primary carbapenemase genes in carbapenem-resistant Klebsiella pneumoniae (CRKp) isolates, highlighting their significant role in carbapenem resistance.
Characterization of Klebsiella pneumoniae Isolates Resistant to Cefiderocol from Hospitals and Outpatient Settings in Croatia.
The study characterizes AMR genes in FDC-resistant K. pneumoniae isolates, identifying bla OXA-48, bla KPC, bla NDM, bla CTX-M, aac(6')-Ib, aadA1, aadA2, qnrB, shv, and tem as significant contributors to resistance.
Characterization of Klebsiella pneumoniae Isolates Resistant to Cefiderocol from Hospitals and Outpatient Settings in Croatia.
The study characterizes AMR genes in FDC-resistant K. pneumoniae isolates, identifying bla OXA-48, bla KPC, bla NDM, bla CTX-M, aac(6')-Ib, aadA1, aadA2, qnrB, shv, and tem as significant contributors to resistance.
Global emergence of Carbapenem-resistant Hypervirulent Klebsiella pneumoniae driven by an IncFII(K34) KPC-2 plasmid.
The study identifies an IncFII K34 KPC-2 plasmid as a major driver of carbapenem resistance in hypervirulent Klebsiella pneumoniae (CR-hvKp), highlighting its high conjugation frequency and increased bla KPC-2 expression compared to other plasmids.
Molecular characterization of resistance and biofilm genes of ESKAPE pathogens isolated from clinical samples: examination of the effect of boric acid on biofilm ability by cell culture method.
The study identified various AMR genes in ESKAPE pathogens, including vanA and vanC for vancomycin resistance, blaKPC and blaOXA-48 for carbapenem resistance, and others related to tetracycline, sulfonamide, quinolone, and aminoglycoside resistance. Additionally, biofilm-related genes such as icaA, icaD, gelE, asa1, and esp were detected in Staphylococcus and Enterococcus spp. Quorum sensing genes LasI and LasR were found in Pseudomonas aeruginosa, and biofilm production genes like mrkA, wbbM, wzm, and luxS were identified in Klebsiella pneumoniae.
Carbapenem-resistant Morganella morganii carrying bla(KPC-2) or bla(NDM-1) in the clinic: one-decade genomic epidemiology analysis.
The study identifies bla(KPC-2) and bla(NDM-1) as the primary carbapenem resistance genes in carbapenem-resistant Morganella morganii (CRMM) isolates, highlighting their role in the spread of resistance through plasmid-mediated mechanisms.
Carbapenem-resistant Morganella morganii carrying bla(KPC-2) or bla(NDM-1) in the clinic: one-decade genomic epidemiology analysis.
The study identifies bla(KPC-2) and bla(NDM-1) as the primary carbapenem resistance genes in carbapenem-resistant Morganella morganii (CRMM) isolates, highlighting their role in the spread of resistance through plasmid-mediated mechanisms.
Wastewater based genomic surveillance key to population level monitoring of AmpC/ESBL producing Escherichia coli.
The study identified various AMR genes and mutations in AmpC/ESBL-producing E. coli from wastewater samples in Finland, highlighting the prevalence of blaCTX-M-15, blaCTX-M-27, and other resistance determinants.
Identification of CMY-190, a novel chromosomally encoded AmpC β-lactamase, and plasmid-encoded KPC-2 in a clinical isolate of Citrobacter youngae.
The study identifies a novel chromosomally encoded AmpC β-lactamase gene, bla CMY-190, and a plasmid-encoded carbapenemase gene, bla KPC-2, in a clinical isolate of Citrobacter youngae.
Phenotype and genotype of carbapenem-resistant hypervirulent Klebsiella pneumoniae in a teaching hospital in Shanghai, China.
The study identified carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) strains carrying blaKPC-2, blaCTX-M-65, and blaTEM-1B genes, which conferred resistance to carbapenems and beta-lactams. These strains also exhibited high virulence.
Diverse evolutionary trajectories of Klebsiella pneumoniae carbapenemase: unraveling the impact of amino acid substitutions on β-lactam susceptibility and the role of avibactam in driving resistance.
The study characterizes several KPC variants, including blaKPC-2, blaKPC-3, blaKPC-4, blaKPC-5, blaKPC-6, blaKPC-14, blaKPC-28, and blaKPC-31, which confer resistance to ceftazidime and ceftazidime-avibactam. Specific mutations such as P104R, V240G, and H274Y were identified as contributing to increased resistance.
Carbapenem-Resistant, Virulence Plasmid-Harboring Klebsiella pneumoniae, United States.
The study identifies six pVir-CRKP isolates from the United States, highlighting their multidrug resistance and enhanced virulence traits. These isolates exhibit resistance to various antibiotics, including carbapenems, cephalosporins, fluoroquinolones, and aminoglycosides, due to the presence of specific AMR genes and mutations.
Evaluating the Performance of Two Rapid Immunochromatographic Techniques for Detecting Carbapenemase in Carbapenem-Resistant Enterobacterales Clinical Isolates.
The study identified a novel bla KPC gene, bla KPC-145, which contains a point mutation (A to G) at nucleotide position 787, resulting in the amino acid substitution of threonine to alanine (T263A). This variant was accurately detected by the K-Set but not by Carba 5.
Next-generation diagnostics of bloodstream infections enabled by rapid whole-genome sequencing of bacterial cells purified from blood cultures.
The study presents a rapid whole-genome sequencing workflow (LC-WGS) for diagnosing bloodstream infections, demonstrating accurate identification of bacterial pathogens and detection of clinically relevant resistance markers within 4.2 hours. The workflow successfully identified various AMR genes, including bla CTX-M-15, bla DHA-1, bla KPC-2, bla KPC-3, bla NDM-1, bla OXA-23, armA, mecA, vanRSHAXYZ, aac(6')-Ie/aph(2'')-Ia, aph(3')-IIIa, aac(6')-I, sul1, and dfrA17.
Next-generation diagnostics of bloodstream infections enabled by rapid whole-genome sequencing of bacterial cells purified from blood cultures.
The study presents a rapid whole-genome sequencing workflow (LC-WGS) for diagnosing bloodstream infections, demonstrating accurate identification of bacterial pathogens and detection of clinically relevant resistance markers within 4.2 hours. The workflow successfully identified various AMR genes, including bla CTX-M-15, bla DHA-1, bla KPC-2, bla KPC-3, bla NDM-1, bla OXA-23, armA, mecA, vanRSHAXYZ, aac(6')-Ie/aph(2'')-Ia, aph(3')-IIIa, aac(6')-I, sul1, and dfrA17.
Characterization of carbapenem-resistant Klebsiella pneumoniae in bloodstream infections: antibiotic resistance, virulence, and treatment strategies.
The study identified bla KPC and bla NDM as the primary carbapenem resistance genes in CRKP isolates, with bla KPC being the most prevalent. Additionally, virulence genes uge and wabG were highly prevalent, suggesting their role in persistence and transmission.
Rapid prediction of carbapenemases in Pseudomonas aeruginosa by imipenem/relebactam and MALDI-TOF MS.
The study characterizes various carbapenemase genes such as blaIMP-13, blaIMP-94, blaNDM-1, blaNDM-5, blaNDM-7, blaNDM-23, blaVIM-1, blaVIM-2, blaVIM-20, blaKPC-2, blaKPC-3, blaGES-1, blaGES-5, blaGES-7, blaGES-20, blaPER-1, blaVEB-1, blaCTX-M-15, blaCTX-M-9, blaSHV-12, blaFOX-4, blaCMY-2, blaDHA-1, blaOXA-2, blaOXA-10, blaOXA-14, blaOXA-15, and blaOXA-48 in Pseudomonas aeruginosa using MALDI-TOF MS hydrolysis assays.
Rapid prediction of carbapenemases in Pseudomonas aeruginosa by imipenem/relebactam and MALDI-TOF MS.
The study characterizes various carbapenemase genes such as blaIMP-13, blaIMP-94, blaNDM-1, blaNDM-5, blaNDM-7, blaNDM-23, blaVIM-1, blaVIM-2, blaVIM-20, blaKPC-2, blaKPC-3, blaGES-1, blaGES-5, blaGES-7, blaGES-20, blaPER-1, blaVEB-1, blaCTX-M-15, blaCTX-M-9, blaSHV-12, blaFOX-4, blaCMY-2, blaDHA-1, blaOXA-2, blaOXA-10, blaOXA-14, blaOXA-15, and blaOXA-48 in Pseudomonas aeruginosa using MALDI-TOF MS hydrolysis assays.
Whole-Genome Sequencing of Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Isolated from Human Bloodstream Infections.
The study identified multiple AMR genes in ESBL-producing K. pneumoniae isolates, including blaCTX-M-15, blaKPC-3, and others conferring resistance to β-lactams, aminoglycosides, fluoroquinolones, sulfonamides, tetracyclines, and chloramphenicol.
Emergence of Tigecycline-Nonsusceptible Carbapenem-Resistant Klebsiella pneumoniae with Metallo-β-Lactamase and Transferable Ceftazidime-Avibactam Resistance in China.
The study identifies multiple AMR genes and mutations in MBL-CRKP strains, including blaIMP-4, blaNDM-1, blaNDM-29, and tmexCD2-toprJ2, which confer resistance to carbapenems, CZA, and tigecycline. Mutations in rpsJ, tet(A), ompK35, and ompK36, along with upregulation of efflux pump genes, contribute to tigecycline resistance.
Impact of the Technical Snow Production Process on Bacterial Community Composition, Antibacterial Resistance Genes, and Antibiotic Input-A Dual Effect of the Inevitable.
The study identified several antibiotic resistance genes (ARGs) in water and snow samples from ski resorts, including blaTEM, blaCTX-M, mecA, ereA, ermB, strA, tetK, and sulIII. These genes were associated with resistance to beta-lactams, macrolides, aminoglycosides, tetracyclines, and sulfonamides. The presence of these ARGs highlights the potential environmental impact of technical snow production on antimicrobial resistance.
In-depth characterization of Klebsiella pneumoniae carbapenemase (KPC)-encoding plasmids points at transposon-related transmission of resistance genes.
The study identifies a blaKPC-3 gene encoded on an IncF plasmid in K. pneumoniae isolates, highlighting the role of transposons in the transmission of carbapenem resistance.
Polyclonal carbapenemase-producing Escherichia coli in Northern Italy: the emergence of NDM-7.
The study identifies the emergence of NDM-7 in polyclonal carbapenemase-producing E. coli in Northern Italy, highlighting the presence of various carbapenemase genes such as bla KPC-3, bla VIM-1, and bla NDM-7, along with other resistance genes.
Unraveling the genomic epidemiology and plasmid-mediated carbapenem resistance of Klebsiella pasteurii.
The study identified the carbapenem resistance gene blaKPC-2 in a Klebsiella pasteurii strain, K1134, which was resistant to meropenem and imipenem. The gene was found on a plasmid pK1134-KPC belonging to the IncFII pCP020359 group.
Spectrum of cefepime-taniborbactam coverage against 190 β-lactamases defined in engineered isogenic Escherichia coli strains.
Spectrum of cefepime-taniborbactam coverage against 190 β-lactamases defined in engineered isogenic Escherichia coli strains.
Spectrum of cefepime-taniborbactam coverage against 190 β-lactamases defined in engineered isogenic Escherichia coli strains.
Spectrum of cefepime-taniborbactam coverage against 190 β-lactamases defined in engineered isogenic Escherichia coli strains.
Spectrum of cefepime-taniborbactam coverage against 190 β-lactamases defined in engineered isogenic Escherichia coli strains.
Spectrum of cefepime-taniborbactam coverage against 190 β-lactamases defined in engineered isogenic Escherichia coli strains.
Spectrum of cefepime-taniborbactam coverage against 190 β-lactamases defined in engineered isogenic Escherichia coli strains.
Evaluation of the multiplex PCR combined with capillary electrophoresis technique for detecting pathogenic bacteria and antibiotic resistance genes in bone infections.
The study evaluated the performance of mPCR-CE for detecting pathogens and antibiotic resistance genes in bone infections, highlighting the effectiveness of the method in identifying multidrug-resistant organisms such as MRSA, ESBL-producing bacteria, and carbapenem-resistant Enterobacteriaceae.
Evaluation of phenotypic and genotypic methods for detecting KPC variants.
The study characterizes 45 KPC variants, identifying different resistance profiles and evaluating the effectiveness of various detection methods. Key findings include the identification of 'KPC-like', 'ESBL-like', 'ceftazidimase', and 'mixed' profiles, with variations in resistance to beta-lactams and carbapenems.
Evaluation of phenotypic and genotypic methods for detecting KPC variants.
The study characterizes 45 KPC variants, identifying different resistance profiles and evaluating the effectiveness of various detection methods. Key findings include the identification of 'KPC-like', 'ESBL-like', 'ceftazidimase', and 'mixed' profiles, with variations in resistance to beta-lactams and carbapenems.
Use of MALDI-TOF VITEK MS for rapid and efficient identification of KPC-type carbapenemases in Enterobacterales carrying the Tn4401a transposon.
The study validated the use of MALDI-TOF VITEK MS for detecting KPC-type carbapenemases in Enterobacterales carrying the Tn4401a transposon by identifying the 11,109 Da peak in mass spectra.
Study on the invitro synergistic susceptibility and biofilm inhibition mechanism of ceftazidime-avibactam combined with aztreonam against carbapenem-resistant Klebsiella pneumoniae.
The study identifies blaKPC-2 and blaNDM-1 as the predominant carbapenemase genes in CRKP isolates, demonstrating the synergistic antibacterial effect of CZA and ATM against CRKP strains with various enzyme types, especially those carrying blaKPC-2.
Genomic characteristics, virulence potential, antimicrobial resistance profiles, and phylogenetic insights into Nocardia cyriacigeorgica.
The study identified several antimicrobial resistance genes in Nocardia cyriacigeorgica, including VanSO and VanRO for vancomycin resistance, erm(O)-Irm, srmB, and ermH for macrolide resistance, bla1 and bcl for beta-lactam resistance, CTX-M and KPC for cephalosporin and carbapenem resistance, tetA(58) and tetB(58) for tetracycline resistance, folC and folP for sulfonamide resistance, rbpA and rpoB2 for rifamycin resistance, murA and AbaF for fosfomycin resistance, cmlR for chloramphenicol resistance, and VatF and VatE for streptogramin resistance.
Assessment of the Diagnostic Accuracy of the Modified Hodge Test and Modified Carbapenem Inactivation Method for Identifying Carbapenem Resistance Mechanisms in Klebsiella pneumoniae: A Whole Genome Sequencing-Based Exploratory Study.
The study identified bla OXA-48-like, bla NDM-1, bla NDM-5, and bla KPC genes as the main carbapenem resistance mechanisms in Klebsiella pneumoniae isolates, with bla OXA-48-like being the most prevalent.
The importance of monitoring a new antibiotic: ceftazidime/avibactam usage and resistance experience from England, 2016 to 2020.
The study identified the presence of ceftazidime/avibactam resistance in Enterobacterales in England, with nearly 90% of resistance cases associated with metallo-beta-lactamase (MBL) genes such as bla KPC, bla OXA-48-like, bla NDM, bla VIM, and bla IMP. These genes were experimentally validated as conferring resistance to ceftazidime/avibactam.
Characterization of phenotypic and genotypic traits of Klebsiella pneumoniae strains resistant to 3rd generation cephalosporins in hospital settings: A case study in Ho Chi Minh City, Vietnam.
The study identifies the prevalence of AmpC beta-lactamase, ESBL, and carbapenemase genes in Klebsiella pneumoniae isolates from Ho Chi Minh City, Vietnam, highlighting the high resistance rates to third-generation cephalosporins and carbapenems.
Diagnostic algorithm for the detection of carbapenemases and extended-spectrum β-lactamases in carbapenem-resistant Pseudomonas aeruginosa.
The study identifies several carbapenemase and extended-spectrum beta-lactamase genes, including bla(VIM-2), bla(VIM-4), bla(IMP-1), bla(NDM-1), bla(GES-5), and bla(KPC-2), which confer resistance to carbapenems and other beta-lactam antibiotics in carbapenem-resistant Pseudomonas aeruginosa.
Diagnostic algorithm for the detection of carbapenemases and extended-spectrum β-lactamases in carbapenem-resistant Pseudomonas aeruginosa.
The study identifies several carbapenemase and extended-spectrum beta-lactamase genes, including bla(VIM-2), bla(VIM-4), bla(IMP-1), bla(NDM-1), bla(GES-5), and bla(KPC-2), which confer resistance to carbapenems and other beta-lactam antibiotics in carbapenem-resistant Pseudomonas aeruginosa.
The ongoing antibiotic resistance and carbapenemase encoding genotypes surveillance. The first quarter report of the INVIFAR network for 2024.
The study identifies bla NDM, bla KPC, bla OXA24, and bla GES as carbapenemase-encoding genes in various bacterial species, highlighting their role in carbapenem resistance.
Emergence and characterization of IncFII/IncR plasmids with multiple 5,692 bp- bla (KPC-2)-bearing tandem repeats in ceftazidime/avibactam non-susceptible Klebsiella pneumoniae strains.
The study identifies the blaKPC-2 gene as a critical factor in carbapenem and ceftazidime/avibactam resistance in Klebsiella pneumoniae strains, highlighting the role of plasmid-mediated gene duplication in resistance development.
Phenotype Detection and Drug Resistance Analysis of Carbapenem-Resistant Gram-Negative Bacilli.
The study identified NDM, KPC, and OXA-23 carbapenemase genes in various Gram-negative bacilli, demonstrating their role in carbapenem resistance through PCR detection.
Clinical and Microbiological characteristics of patients with ceftazidime/avibactam-resistant Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae strains.
The study identified KPC variants, including KPC-33, KPC-35, and others, which are associated with ceftazidime/avibactam resistance and restored meropenem susceptibility in K. pneumoniae strains.
Emergence of KPC-8-producing K. pneumoniae infection without prior exposure to ceftazidime/avibactam: the threat of de novo infections by ceftazidime/avibactam-resistant KPC variants.
The study characterizes a KPC-8-producing Klebsiella pneumoniae strain that exhibits resistance to ceftazidime/avibactam without prior exposure to the drug, highlighting the emergence of de novo infections by ceftazidime/avibactam-resistant KPC variants.
Comparison of CRISPR-Cas9, CRISPR-Cas12f1, and CRISPR-Cas3 in eradicating resistance genes KPC-2 and IMP-4.
The study demonstrates that CRISPR-Cas9, CRISPR-Cas12f1, and CRISPR-Cas3 can effectively eradicate the carbapenem resistance genes KPC-2 and IMP-4 in E. coli, restoring bacterial susceptibility to antibiotics.
Identification of pandemic ST147, ESBL-type β-lactamases, carbapenemases, and virulence factors in Klebsiella pneumoniae isolated from southern Peru.
The study identified ESBL genes (blaCTX-M, blaTEM, blaSHV) and carbapenemase genes (blaKPC-2, blaNDM-1) in K. pneumoniae isolates from Cusco, Peru. Additionally, aminoglycoside resistance genes (aadA1, aph(3')-Ib) and mutations in porin-coding genes (ompK36) and gyrA were found, contributing to multidrug resistance.
Mobile genetic elements in Klebsiella pneumoniae.
The paper characterizes several AMR genes including bla KPC, bla NDM-5, mcr-1, mcr-7, mcr-8, tet(X), and tmexCD1-toprJ1, highlighting their role in carbapenem, colistin, and tigecycline resistance in Klebsiella pneumoniae.
Identification of a Potential High-Risk Clone and Novel Sequence Type of Carbapenem-Resistant Pseudomonas aeruginosa in Metro Manila, Philippines.
The study identifies OprD mutations as a significant mechanism of carbapenem resistance in Pseudomonas aeruginosa isolates from the Philippines, highlighting the importance of porin dysfunction in resistance development.
First Detection of International High-Risk bla (KPC-2)-Harbouring Escherichia coli Pandemic Lineage ST648 in Pet Food Packages.
The study detected blaKPC-2-harboring E. coli ST648 in pet food, highlighting the potential transmission of multidrug-resistant pathogens through pet food.
Wild Birds as Drivers of Salmonella Braenderup and Multidrug Resistant Bacteria in Wetlands of Northern Italy.
The study identifies several AMR genes in bacterial isolates from wild aquatic birds in Northern Italy, including beta-lactamases (blaTEM, blaCMY-1, blaCMY-2, blaNDM, blaKPC), colistin resistance genes (mcr-2, mcr-3, mcr-4), tetracycline resistance genes (tetA, tetB, tetC, tetL, tetM, tetK), macrolide resistance genes (ermB, vatD, vgA, msrC), vancomycin resistance genes (vanC1, vanC2, vanM, vanG), sulfonamide resistance genes (sul1, sul2, sul3), aminoglycoside resistance genes (aac(3), aac(6')-Ib, aph(3')-Ia, armA, rmtB, rmtC, rmtF), and nitrofurantoin resistance genes (nfsA, nfsB).
In vitro Evaluation of the Antibacterial Activity of the Combination of Avibactam and β-Lactams Against Highly Virulent Carbapenem-Resistant Klebsiella pneumoniae.
The study identified blaKPC-2 and blaNDM as the primary carbapenem resistance genes in CRKP strains, highlighting their significance in resistance mechanisms.
Genomic insights into the rapid rise of Pseudomonas aeruginosa ST463: A high-risk lineage's adaptive strategy in China.
The study identifies blaKPC-2, blaKPC-33, and blaKPC-90 as key carbapenem resistance genes in Pseudomonas aeruginosa ST463, along with mutations in ftsL and gyrA contributing to carbapenem and fluoroquinolone resistance.
Genomic insights into the rapid rise of Pseudomonas aeruginosa ST463: A high-risk lineage's adaptive strategy in China.
The study identifies blaKPC-2, blaKPC-33, and blaKPC-90 as key carbapenem resistance genes in Pseudomonas aeruginosa ST463, along with mutations in ftsL and gyrA contributing to carbapenem and fluoroquinolone resistance.
Genomic insights into the rapid rise of Pseudomonas aeruginosa ST463: A high-risk lineage's adaptive strategy in China.
The study identifies blaKPC-2, blaKPC-33, and blaKPC-90 as key carbapenem resistance genes in Pseudomonas aeruginosa ST463, along with mutations in ftsL and gyrA contributing to carbapenem and fluoroquinolone resistance.
Emerging Resistance to Novel β-Lactam beta-lactamase Inhibitor Combinations in Klebsiella pneumoniae bearing KPC Variants.
This study identifies emerging resistance to novel β-lactam beta-lactamase inhibitor combinations in Klebsiella pneumoniae bearing KPC variants, highlighting the development of collateral resistance to cefiderocol (FDC) and cefepime/zidebactam (FPZ).
Impact of the double deletion ΔG242-T243 in KPC-2 in the effectiveness of ceftazidime-avibactam and imipenem-relebactam.
The study identifies the KPC-14 variant, which has a double deletion (ΔG242-T243) that increases resistance to ceftazidime-avibactam and reduces the effectiveness of imipenem-relebactam.
Impact of the double deletion ΔG242-T243 in KPC-2 in the effectiveness of ceftazidime-avibactam and imipenem-relebactam.
The study identifies the KPC-14 variant, which has a double deletion (ΔG242-T243) that increases resistance to ceftazidime-avibactam and reduces the effectiveness of imipenem-relebactam.
Simultaneous detection of pathogens and antimicrobial resistance genes with the open source, cloud-based, CZ ID platform.
The study highlights the integration of pathogen detection and AMR profiling using the CZ ID platform, demonstrating the ability to detect various AMR genes such as bla, aac(6')-Ic, mecA, mcr, blaCTX-M, blaNDM, blaKPC, and ampC in clinical and environmental samples.
REL/DPA/AVI method: a novel approach for rapid detection of carbapenemase-producing Enterobacterales directly from positive blood cultures based on optical density.
The REL/DPA/AVI method was developed for rapid detection of carbapenemase-producing Enterobacterales directly from positive blood cultures. It showed high sensitivity and specificity for detecting class A, B, and D carbapenemases, with improved time efficiency compared to traditional methods.
REL/DPA/AVI method: a novel approach for rapid detection of carbapenemase-producing Enterobacterales directly from positive blood cultures based on optical density.
The REL/DPA/AVI method was developed for rapid detection of carbapenemase-producing Enterobacterales directly from positive blood cultures. It showed high sensitivity and specificity for detecting class A, B, and D carbapenemases, with improved time efficiency compared to traditional methods.
Antifungal agent tavaborole as a potential broad-spectrum serine and metallo-β-lactamases inhibitor.
Tavaborole was identified as a broad-spectrum inhibitor of serine and metallo-beta-lactamases, enhancing the efficacy of beta-lactam antibiotics against resistant strains.
Characterization of a bla(KPC-3)-carrying plasmid in a clinical isolate of Klebsiella pneumoniae belonging to the emerging successful clone ST147.
The study characterizes a bla(KPC-3)-carrying plasmid in a clinical isolate of Klebsiella pneumoniae ST147, highlighting its resistance to carbapenems and other antibiotics.
Emergence and characterization of a ST852 Klebsiella quasipneumoniae clinical isolate coharboring bla (NDM-1) and bla (KPC-2) in China.
The study reports the first characterization of a ST852 Klebsiella quasipneumoniae isolate co-harboring bla NDM-1 and bla KPC-2, highlighting its extensive drug-resistant profile and the presence of resistance genes on plasmids.
Microbiological Risks to Health Associated with the Release of Antibiotic-Resistant Bacteria and β-Lactam Antibiotics Through Hospital Wastewater.
The study identifies the presence of β-lactam resistance genes such as bla KPC, bla OXA-48, bla NDM, and bla VIM in various bacterial isolates from hospital wastewater, highlighting the risk of antibiotic-resistant pathogens and the need for improved wastewater management.
Inhibitory activity of meso-dimercaptosuccinic acid against IMP metallo-β-lactamase variants in Pseudomonas aeruginosa.
The study identifies that meso-dimercaptosuccinic acid (DMSA) enhances the efficacy of ceftazidime (CAZ) and cefepime (FEP) against IMP-producing Pseudomonas aeruginosa by inhibiting IMP metallo-β-lactamase variants, demonstrating significant synergistic effects.
Molecular characterization of carbapenem resistance mechanisms and phenotypic correlations in clinical Klebsiella pneumoniae isolates from Ningbo, China.
The study identifies KPC-2, NDM-5, IMP-4, OXA-232, and OXA-181 as the main carbapenemase genes in CRKP isolates from Ningbo, China. It also highlights the prevalence of CTX-M-14, SHV-11, and SHV-12 beta-lactamase genes.
Analysis of rectal carbapenem-resistant Enterobactericeae colonization results first report in Istanbul/Turkiye: Klebsiella pneumoniae co-producing bla(KPC) + bla(NDM) + bla(OXA-48) in a single strain.
The study identified the co-production of bla(KPC), bla(NDM), and bla(OXA-48) in three patients, highlighting the emergence of multidrug-resistant Klebsiella pneumoniae in Istanbul, Turkey.
Diverse modes of ceftazidime/avibactam resistance acquisition in carbapenem-resistant Klebsiella pneumoniae and Pseudomonas aeruginosa from a Chinese intensive care unit.
The study identifies bla KPC-87, bla AFM-2, and bla PER-1 as key genes responsible for ceftazidime/avibactam resistance in carbapenem-resistant Pseudomonas aeruginosa and Klebsiella pneumoniae. Additionally, the GT241A substitution in KPC-87 and the presence of Δ bla TEM-1 contribute to resistance mechanisms.
Clonal dissemination and persistence of Carbapenem-resistant bla (KPC-2) harbouring Klebsiella pneumoniae ST307 in a Tertiary Hospital in the Republic of Korea.
Carbapenem-resistance in Acinetobacter baumannii: prevalence, antibiotic resistance profile and carbapenemase genes in clinical and hospital environmental strains.
The study identified bla KPC, bla VIM, and bla IMP carbapenemase genes in Acinetobacter baumannii isolates from clinical and hospital environmental samples, highlighting the prevalence of multidrug-resistant strains and the importance of monitoring carbapenem resistance.
Prevalence and molecular characterisation of multi-drug resistant ST11 hypervirulent Klebsiella pneumoniae in a teaching hospital.
The study identifies several AMR genes in CRKP strains, including bla KPC-2, bla NDM-1, bla VIM, bla SHV-12, bla TEM-1b, aac(6')-Ib-cr, qnrS, qnrB, rmtB, ant(3'')-I, armA, sul1, sul2, iucA, iutA, iroN, ybtS, mrkD, fimH, and p rmpA. These genes confer resistance to carbapenems, quinolones, aminoglycosides, and sulfonamides. Additionally, the study highlights the transferability of these genes through conjugation.
Diversity of mobile genetic elements in carbapenem-resistant Enterobacterales isolated from the intensive care units of a tertiary care hospital in Northeast India.
The study identified various carbapenemase genes, including bla NDM-1, bla NDM-5, bla NDM-7, bla KPC-2, bla OXA-181, and bla OXA-232, in carbapenem-resistant Enterobacterales isolated from intensive care units in Northeast India.
Diversity of mobile genetic elements in carbapenem-resistant Enterobacterales isolated from the intensive care units of a tertiary care hospital in Northeast India.
The study identified various carbapenemase genes, including bla NDM-1, bla NDM-5, bla NDM-7, bla KPC-2, bla OXA-181, and bla OXA-232, in carbapenem-resistant Enterobacterales isolated from intensive care units in Northeast India.
Detection of KPC-producing Enterobacterales species in wastewater samples from the Gran Concepción Metropolitan area, Chile.
The study identified three carbapenem-resistant Enterobacterales isolates carrying the bla KPC-2 gene, which confers resistance to carbapenems. These isolates were found in wastewater samples from the Gran Concepción Metropolitan area, Chile.
Evaluation of QuickMIC system for rapid antimicrobial susceptibility testing of Gram-negative pathogens from positive blood cultures, including strains producing extended-spectrum β-lactamases and carbapenemases.
The study evaluated the QuickMIC system for rapid antimicrobial susceptibility testing of Gram-negative pathogens, including strains producing extended-spectrum β-lactamases (ESBLs) and carbapenemases. It identified several β-lactamase genes such as bla CTX-M, bla KPC, bla NDM, bla VIM, bla OXA-23-like, bla FIM, and bla VEB, which confer resistance to various beta-lactam antibiotics.
Antimicrobial resistance and phylogenetic lineages of KPC-2-producing blood-borne Klebsiella pneumoniae subsp. pneumoniae from Kolkata, India during 2015-2024: Emergence of Klebsiella pneumoniae subsp. pneumoniae with bla(KPC-2), bla(NDM), and bla(OXA-48-like) triple carbapenemases.
The study identifies blaKPC-2, blaNDM-1, blaNDM-5, blaOXA-181, and blaOXA-232 as key carbapenemase genes in KPC-2-producing Klebsiella pneumoniae subsp. pneumoniae isolates from Kolkata, India. Triple carbapenemase production was observed in some isolates, contributing to multidrug resistance.
From genomics to treatment: overcoming pan-drug-resistant Klebsiella pneumoniae in clinical settings.
The study identifies bla SHV-231 and bla NDM-5 as key drivers of pan-β-lactam resistance in K. pneumoniae, along with mutations in PBP2, PBP3, PmrB, and rpsJ that contribute to resistance against various antibiotics.
Detection of β-lactam resistance genes in Gram-negative bacteria from positive blood cultures using a microchip-based molecular assay.
The study evaluated the Alifax GNR microchip assay for detecting β-lactam resistance genes in Gram-negative bacteria from positive blood cultures, demonstrating high accuracy and broader coverage compared to existing methods.
Molecular and epidemiological characterization of carbapenem-resistant hypervirulent Klebsiella pneumoniae in Huaian, China (2022-2024): a retrospective study.
The study identified blaKPC-2 as the most common carbapenemase in carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) isolates, with all CR-hvKP strains carrying this gene. The ST11 lineage harboring blaKPC-2 was found to be a dominant high-risk clone in the region.
Detection of KPC-Producing Carbapenem-Resistant Klebsiella pneumoniae Based on CRISPR Cas12a.
The study developed a fluorescence-CRISPR Cas12a assay for detecting the KPC gene in carbapenem-resistant Klebsiella pneumoniae, demonstrating high sensitivity and specificity.
Evolution of ceftazidime-avibactam resistance driven by variation in bla (KPC-2) to bla (KPC-190) during treatment of ST11-K64 hypervirulent Klebsiella pneumoniae.
The study identifies a novel KPC-190 variant in a hypervirulent ST11-K64 K. pneumoniae strain during CZA therapy, which confers resistance to CZA while partially restoring carbapenem susceptibility.
Dissemination of KPC-2-producing carbapenem-resistant Klebsiella pneumoniae ST792 in Southern China.
The study identifies multiple AMR genes, including blaKPC-2, qnrS1, blaCTX-M-3, blaTEM-1B, and blaSHV-1, in KPC-2-producing carbapenem-resistant Klebsiella pneumoniae ST792 isolates, highlighting their multidrug-resistant phenotype and plasmid-mediated transmission.
Application of targeted next-generation sequencing for pathogens diagnosis and drug resistance prediction in bronchoalveolar lavage fluid of pulmonary infections.
The study evaluated the application of targeted next-generation sequencing (tNGS) for diagnosing pathogens and predicting drug resistance in bronchoalveolar lavage fluid (BALF) specimens. tNGS showed higher sensitivity in detecting pathogens and resistance genes compared to conventional microbiological tests (CMTs). Key resistance genes identified include blaNDM, blaKPC, mecA, and 23S rRNA:A2063G.
CRISPR-Cas Dynamics in Carbapenem-Resistant and Carbapenem-Susceptible Klebsiella pneumoniae Clinical Isolates from a Croatian Tertiary Hospital.
The study identified bla OXA-48, bla NDM-1, and bla KPC-2 carbapenemase genes in K. pneumoniae isolates and found that CRISPR-Cas systems were associated with specific carbapenemase profiles, suggesting pressure against bla OXA-48 acquisition.
Genomic evolution and dissemination of non-conjugative virulence plasmid of ST65 carbapenem-resistant and hypervirulent Klebsiella pneumoniae strains in a Chinese hospital.
The study identifies the presence of multiple AMR genes, including blaKPC-2, aac(3)-IId, blaTEM-1, and blaCTX-M-15, in ST65 carbapenem-resistant and hypervirulent Klebsiella pneumoniae strains. It also characterizes virulence genes such as iucABCD-iutA, iroBCD-iroN, rmpA, and rmpA2. The research highlights the role of IncM2 plasmids in mobilizing non-conjugative virulence plasmids and the occurrence of IS 26-mediated plasmid fusion.
Skilled nursing facility wastewater surveillance: a SARS-CoV-2 and antimicrobial resistance detection pilot study.
The study detected bla KPC as the main carbapenemase gene in wastewater samples from a skilled nursing facility, indicating the presence of carbapenem-resistant Enterobacterales.
Analysis of clinical characteristics of patients with coronavirus disease 2019 based on a superior carbapenem-resistant Enterobacterales identification strategy.
The study evaluated the performance of GeneXpert Carba-R and immunochromatography for detecting carbapenemases in 115 gram-negative isolates, focusing on bla KPC, bla NDM, bla OXA-48, bla IMP, and bla VIM genes. It highlighted the importance of accurate detection of carbapenemase-producing Enterobacterales to guide antibiotic treatment in patients with COVID-19.
Validation of immunochromatographic test in broth-enriched rectal swab specimens.
The study validated the O.K.N.V.I. RESIST-5 immunochromatographic test for detecting five common carbapenemases (KPC, NDM, OXA-48, VIM, and IMP) in broth-enriched rectal swabs with 100% sensitivity and specificity after 6 hours of incubation without meropenem.
Potential role of avibactam in restoring susceptibility in Escherichia coli with two copies of bla(KPC-3) and PBP3 mutations.
The study identifies blaKPC-3 as a gene conferring resistance to carbapenems in Escherichia coli and Klebsiella pneumoniae. It also characterizes PBP3 mutations (A233T and I332V) that contribute to ceftazidime resistance.
Cooperative resistance varies among beta-lactamases in E. coli, with some enabling cross-protection and sustained extracellular activity.
The study identifies seven beta-lactamase genes (bla ampC, bla TEM-1, bla CTX-M-15, bla CMY-2, bla NDM-5, bla KPC-2, and bla OXA-181) in E. coli that contribute to cooperative resistance, with varying levels of efficacy in rescuing susceptible cells and facilitating cross-protection against β-lactam antibiotics.
Emergence of ST11-KL64 carbapenem-resistant hypervirulent Klebsiella Pneumoniae isolates harboring bla(KPC-2) and iucA from a tertiary teaching hospital in Western China.
The study identifies the emergence of ST11-KL64 carbapenem-resistant hypervirulent Klebsiella pneumoniae isolates harboring bla(KPC-2) and iucA in a tertiary teaching hospital in Western China.
Nosocomial transmission, adaption and clinical outcomes of carbapenem-resistant hypervirulent Klebsiella pneumoniae.
The study identifies blaKPC-2 and blaNDM-1 as the most prevalent carbapenemase genes in CR-HvKp strains, along with virulence genes rmpA, rmpA2, iroB, and iucA. It highlights the dominance of ST11-KL64 hv-CRKP in nosocomial transmission and its association with higher mortality rates.
A comparative analysis of clinical outcomes in hematological patients afflicted with bacteremia attributable to carbapenem-resistant Klebsiella pneumoniae versus Escherichia coli.
The study identified blaKPC and blaNDM genes as the primary carbapenemase genes in carbapenem-resistant Klebsiella pneumoniae (CRKP) and Escherichia coli (CREC) strains, respectively. These genes confer resistance to carbapenems.
Identification of two KPC variants, KPC-204 and KPC-227, in ST11-K64 Klebsiella pneumoniae during prolonged hospitalization of a single patient.
Identification of two KPC variants, KPC-204 and KPC-227, in ST11-K64 Klebsiella pneumoniae during prolonged hospitalization of a single patient.
Antimicrobial resistance and beta-lactamase gene distribution among clinical isolates: a two-year cohort study.
The study identified several beta-lactamase genes, including blaCTX-M, blaSHV, blaTEM, blaOXA-48, blaKPC, blaVIM, and blaNDM, which are associated with resistance to various antibiotics in E. coli isolates.
Molecular epidemiology and clinical characteristics of carbapenem-resistant Klebsiella pneumoniae bloodstream and pneumonia isolates.
The study identified various carbapenemase genes (blaKPC-2, blaKPC-3, blaOXA-181, blaOXA-232, blaOXA-48, blaNDM-1, blaNDM-4, blaNDM-7, blaIMP-1) and extended-spectrum beta-lactamase genes (ampC, ctx-M-15, shv-9) in carbapenem-resistant Klebsiella pneumoniae isolates. Additionally, mutations in porin genes ompK35 and ompK36 were found to contribute to carbapenem resistance in isolates lacking carbapenemases.
Molecular epidemiology and clinical characteristics of carbapenem-resistant Klebsiella pneumoniae bloodstream and pneumonia isolates.
The study identified various carbapenemase genes (blaKPC-2, blaKPC-3, blaOXA-181, blaOXA-232, blaOXA-48, blaNDM-1, blaNDM-4, blaNDM-7, blaIMP-1) and extended-spectrum beta-lactamase genes (ampC, ctx-M-15, shv-9) in carbapenem-resistant Klebsiella pneumoniae isolates. Additionally, mutations in porin genes ompK35 and ompK36 were found to contribute to carbapenem resistance in isolates lacking carbapenemases.
Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic.
The study characterizes the plasmid diversity in Klebsiella pneumoniae ST307 isolates co-producing KPC and NDM carbapenemases, identifying multiple resistance genes and plasmid types involved in carbapenem resistance.
Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic.
The study characterizes the plasmid diversity in Klebsiella pneumoniae ST307 isolates co-producing KPC and NDM carbapenemases, identifying multiple resistance genes and plasmid types involved in carbapenem resistance.
Transcriptomic Insights into Adaptive Strategies of Klebsiella pneumoniae Co-Producing KPC-2 and NDM-5 Carbapenemases Under Meropenem Stress.
The study identifies bla KPC-2 and bla NDM-5 as key carbapenemase genes in K. pneumoniae KP4, contributing to pan-drug resistance. The strain exhibits high meropenem MIC (512 mg/L) and enhanced biofilm formation under meropenem stress, indicating dual resistance mechanisms.
Emerging Trends of Beta-Lactamases in Western Nepal: A Growing Public Health Concern.
The study identified the prevalence of ESBL, AmpC, and carbapenemase-producing clinical isolates of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. ESBL production was the predominant mechanism of resistance to beta-lactam drugs, followed by AmpC and carbapenemase production.
Evolution of XDR Pseudomonas aeruginosa ST463 strains with two plasmids harboring multiple antimicrobial resistance genes.
The study identifies multiple antimicrobial resistance genes, including blaIMP-45, blaAFM-1, and blaKPC-2, in XDR Pseudomonas aeruginosa ST463 strains, highlighting their role in carbapenem resistance.
Carbapenem-Resistant Gram-Negative Bacteria in Hospitalized Patients: A Five-Year Surveillance in Italy.
The study identifies the presence of carbapenem-resistant Klebsiella pneumoniae isolates carrying bla KPC, bla NDM, bla VIM, and bla IMP genes, which confer resistance to carbapenems.
Comparative genomics of Pseudomonas paraeruginosa.
The study identifies various AMR genes and mutations in Pseudomonas paraeruginosa, including carbapenemases like blaVIM-2, blaVIM-6, blaVIM-28, and blaKPC-2, as well as efflux pump genes (mexAB-oprM, mexCD-oprJ, etc.), and mutations in oprD, mexS, mexR, mexZ, lasR, mvfR, and vqsM that contribute to antibiotic resistance.
Study on Antibacterial Activity of Water Extract of Galla chinensis Against Carbapenemase-Producing Enterobacteriaceae.
The study evaluated the antibacterial activity of Galla chinensis (GC) water extract against carbapenemase-producing Enterobacteriaceae (CPE) and found that it significantly inhibited bacterial growth and reduced the activity of carbapenemase genes.
A network analysis of carbapenem-resistant Klebsiella pneumoniae among healthcare facilities.
The study identifies bla KPC-2 and bla KPC-3 as the most common carbapenemase genes in carbapenem-resistant Klebsiella pneumoniae isolates, highlighting their role in resistance.
A network analysis of carbapenem-resistant Klebsiella pneumoniae among healthcare facilities.
The study identifies bla KPC-2 and bla KPC-3 as the most common carbapenemase genes in carbapenem-resistant Klebsiella pneumoniae isolates, highlighting their role in resistance.
The hospital sink drain microbiome as a melting pot for AMR transmission to nosocomial pathogens.
The study highlights the hospital sink drain microbiome as a significant reservoir for antimicrobial resistance genes (ARGs), including bla KPC, bla NDM-1, bla OXA-48, and bla IMP-8, which confer resistance to carbapenems. These genes are found in various opportunistic pathogens and are transmitted through horizontal gene transfer, emphasizing the role of sink drains in the spread of multidrug-resistant organisms.
Exploring Virulence Characteristics of Clinical Escherichia coli Isolates from Greece.
The study identified several beta-lactamase genes (bla CTX-M-15, bla CTX-M-14, bla CTX-M-27, bla NDM-1, bla KPC-2) and the colistin resistance gene mcr1.1 in E. coli isolates, highlighting the presence of multidrug resistance.
Genomic characterization of a multidrug-resistant Citrobacter portucalensis isolate co-harboring bla (KPC-2) and bla (NDM-1) on distinct plasmids.
The study identifies a multidrug-resistant Citrobacter portucalensis isolate co-harboring bla KPC-2 and bla NDM-1 on distinct plasmids, along with additional resistance genes on the chromosome and plasmids, highlighting the potential for horizontal gene transfer and the need for enhanced surveillance.
Genomic characterization of a multidrug-resistant Citrobacter portucalensis isolate co-harboring bla (KPC-2) and bla (NDM-1) on distinct plasmids.
The study identifies a multidrug-resistant Citrobacter portucalensis isolate co-harboring bla KPC-2 and bla NDM-1 on distinct plasmids, along with additional resistance genes on the chromosome and plasmids, highlighting the potential for horizontal gene transfer and the need for enhanced surveillance.
Metagenomic analysis after selective culture enrichment of hospital and community wastewater enhances antimicrobial resistance gene detection.
The study identifies various beta-lactamase genes such as bla CTX-M, bla KPC, bla NDM, and bla VIM, along with other resistance genes like erm(TR), lsa, efrA, efrB, and optrA, which were detected through selective culture enrichment of wastewater samples.
Epidemiological and biological characteristics of IncR plasmids as multihost antibiotic resistance carriers.
The study characterizes various AMR genes carried by IncR plasmids, highlighting their role in the dissemination of resistance to carbapenems, cephalosporins, fluoroquinolones, aminoglycosides, and tetracyclines.
Plasmid genomic epidemiology of bla (NDM) carbapenemase-producing Enterobacterales in Canada from 2010 to 2023.
The study characterizes various bla NDM and bla OXA-48-type carbapenemase genes in Enterobacterales isolates from Canada, highlighting their diversity and co-harbouring on plasmids.
Incidence, clinical and genomic trends of hospital- and Non-hospital-onset KPC-producing Klebsiella pneumoniae infections before and during the COVID-19 era: a ten-year interrupted time series study.
The study identifies the blaKPC-3 gene as a major contributor to carbapenem resistance in KPC-producing Klebsiella pneumoniae, highlighting the clonal spread of the ST512/KPC-3 lineage across hospital and community settings.
Microbiome-mediated colonization resistance to carbapenem-resistant Klebsiella pneumoniae in ICU patients.
The study identifies blaKPC-2 and blaNDM-1 as carbapenem resistance genes in CRKP isolates, highlighting their role in resistance to meropenem.
ST11 carbapenem-resistant Klebsiella pneumoniae integrates virulence plasmid fragments into the chromosome via insertion sequence.
The study identifies the integration of virulence plasmid fragments into the chromosome of ST11 carbapenem-resistant Klebsiella pneumoniae, highlighting the role of IS26 and ISKpn1 in this process. It characterizes blaKPC-2 and rmpA2 as key genes involved in resistance and virulence.
The clinical impact and dissemination of carbapenemase-producing Enterobacter: a genome-based study in China.
The study identifies blaNDM-1, blaNDM-5, blaIMP-4, blaFRI-11, and blaKPC-2 as the primary carbapenemase genes in carbapenemase-producing Enterobacter (CPEn) isolates in China, predominantly found in E. xiangfangensis and E. hoffmannii. These genes confer resistance to carbapenems and are widespread across various Enterobacter species.
CRISPR-Cas9 targeting the blaKPC gene in a clinical isolate of Klebsiella michiganensis: Reduction of imipenem resistance and changes in genomic carbapenem resistance determinants.
CRISPR-Cas9 targeting the blaKPC gene in Klebsiella michiganensis reduced imipenem resistance through plasmid copy number reduction and decreased blaKPC gene expression. Mutations in ompK36, acrB, and recN were observed, contributing to resistance mechanisms.
Emergence and molecular evolution of carbapenem-resistant hypervirulent ST23 Klebsiella pneumoniae: The superbug phenomenon in China.
The study characterizes the emergence and molecular evolution of carbapenem-resistant hypervirulent ST23 Klebsiella pneumoniae in China, highlighting the presence of various carbapenemase genes such as bla KPC-2, bla NDM, and bla OXA-48, along with virulence factors like rmpA, rmpA2, iroN, iucA, and pagO.
Molecular and virulence differences of Klebsiella pneumoniae isolated from blood.
The study identified the blaKPC-2 gene as a key factor in carbapenem resistance among Klebsiella pneumoniae isolates from blood, highlighting the diversity of resistance mechanisms and plasmid profiles among the strains.
High transconjugation efficiency of fusion plasmid pNDM_KPC in carbapenem-resistant Citrobacter freundii and its formation driven by IS26-mediated integration.
The study identified the presence of a fusion plasmid pNDM_KPC co-harboring bla KPC-2 and bla NDM-1 in carbapenem-resistant Citrobacter freundii isolates, highlighting the potential for rapid dissemination of multidrug resistance.
A novel broad host range phage phiA85 displays a synergistic effect with antibiotics targeting carbapenem-resistant Klebsiella pneumoniae.
The study identified a novel broad host range phage phiA85 that effectively targets multiple carbapenem-resistant Klebsiella pneumoniae (CRKP) strains. Additionally, the phage phiA85 in combination with antibiotics showed synergistic effects against CRKP in vitro and in vivo.
Occurrence of KPC-71 or the presence of NDM combined with a high expression of bla(SHV) contributes to low-level cefiderocol resistance in carbapenem-resistant Klebsiella pneumoniae in China.
The study identifies KPC-71 and NDM, in combination with high expression of bla(SHV), as contributors to low-level cefiderocol resistance in carbapenem-resistant Klebsiella pneumoniae. KPC-71 was shown to significantly decrease cefiderocol susceptibility, while NDM and bla(SHV) also played roles in resistance.
Occurrence of KPC-71 or the presence of NDM combined with a high expression of bla(SHV) contributes to low-level cefiderocol resistance in carbapenem-resistant Klebsiella pneumoniae in China.
The study identifies KPC-71 and NDM, in combination with high expression of bla(SHV), as contributors to low-level cefiderocol resistance in carbapenem-resistant Klebsiella pneumoniae. KPC-71 was shown to significantly decrease cefiderocol susceptibility, while NDM and bla(SHV) also played roles in resistance.
Meropenem/vaborbactam activity against carbapenem-resistant Klebsiella pneumoniae from catheter-related bloodstream infections.
The study identifies blaKPC-3 and blaOXA-1 as resistance determinants in carbapenem-resistant Klebsiella pneumoniae isolates, demonstrating susceptibility to meropenem/vaborbactam.
Prevalence and determinants of multidrug-resistant uropathogenic Klebsiella species and associated antimicrobial resistance genes in Port Harcourt, Nigeria.
The study identified a high prevalence of multidrug-resistant Klebsiella species in Port Harcourt, with blaCTX-M, blaTEM-1, blaSHV, and KPC genes being the primary contributors to resistance.
Phenotypic characterisation and prevalence of carbapenem-resistant Enterobacterales in a tertiary care centre in Bihar India.
The study identifies the prevalence of carbapenem-resistant Enterobacterales (CRE) in Bihar, India, highlighting the dominance of Class B carbapenemases (blaNDM, blaOXA-48, blaIMP, blaKPC, blaVIM) and the co-expression of Class A and Class B carbapenemases in E. coli and K. pneumoniae.
Impact of Dietary Inputs on Carbapenem Resistance Gene Dynamics and Microbial Safety During Bioconversion of Agri-Food Waste and Anaerobic Digestate by Hermetia illucens Larvae.
The study identifies the presence of carbapenem resistance genes (bla VIM, bla OXA-48, bla GES, and bla KPC) in Hermetia illucens larvae and frass, primarily associated with legume-based diets. These genes were not detected in young larvae or in diets containing onion or digestate.
Evaluation of the rapid lateral flow assay (LFA) for detection of five major carbapenemase enzyme families in genotypically characterised bacterial isolates.
The study evaluates a rapid lateral flow assay (LFA) for the detection of five major carbapenemase enzyme families (KPC, NDM, VIM, IMP, and OXA-48-like) in genotypically characterized bacterial isolates. The LFA showed high sensitivity and specificity for detecting these carbapenemases, with some limitations noted for NDM detection.
Evaluation of the rapid lateral flow assay (LFA) for detection of five major carbapenemase enzyme families in genotypically characterised bacterial isolates.
The study evaluates a rapid lateral flow assay (LFA) for the detection of five major carbapenemase enzyme families (KPC, NDM, VIM, IMP, and OXA-48-like) in genotypically characterized bacterial isolates. The LFA showed high sensitivity and specificity for detecting these carbapenemases, with some limitations noted for NDM detection.
Investigation of carbapenemase-encoding genes in Burkholderia cepacia and Aeromonas sobria isolates from nosocomial infections in Iraqi patients.
The study identified bla KPC and bla GES carbapenemase genes in Burkholderia cepacia and Aeromonas sobria isolates, which confer resistance to meropenem and imipenem.
The bacterial etiology and antimicrobial susceptibility of lower respiratory tract infections in Vietnam.
The study identified several AMR genes, including bla CTX-M-1, bla CTX-M-9, bla TEM, bla SHV, bla OXA-23, bla OXA-48, bla NDM, bla KPC, and mecA, which were associated with resistance to various antibiotics in different bacterial pathogens.
Emergence of carbapenem-resistant Serratia marcescns co-harboring bla(NDM-1), bla(KPC-2), and bla(SRT-2) in bloodstream infection.
The study identifies the first case of a carbapenem-resistant Serratia marcescens strain co-harboring bla(NDM-1), bla(KPC-2), and bla(SRT-2) in China, highlighting the potential for horizontal gene transfer and the challenge of managing multidrug-resistant infections.
Rapid detection of carbapenemases in multiresistant Gram-negative strains: evaluation of two tests.
The study evaluated the performance of the NG CARBA-5 test and the Allplex Entero-DR assay for detecting carbapenemases in multidrug-resistant Gram-negative bacteria. Both tests were able to detect various carbapenemase genes, including blaKPC, blaNDM, blaOXA-48, blaVIM, and blaIMP.
High-throughput screening of monoclonal antibodies against carbapenemases using a multiplex protein microarray platform.
The study identifies and characterizes monoclonal antibodies targeting various carbapenemases (bla KPC, bla NDM, bla IMP-1, bla VIM, bla OXA-23, bla OXA-48, bla OXA-58) and the mcr-1 gene, which confers resistance to colistin. These antibodies show high specificity and sensitivity in detecting resistance determinants using a protein microarray platform.
High-throughput screening of monoclonal antibodies against carbapenemases using a multiplex protein microarray platform.
The study identifies and characterizes monoclonal antibodies targeting various carbapenemases (bla KPC, bla NDM, bla IMP-1, bla VIM, bla OXA-23, bla OXA-48, bla OXA-58) and the mcr-1 gene, which confers resistance to colistin. These antibodies show high specificity and sensitivity in detecting resistance determinants using a protein microarray platform.
High-throughput screening of monoclonal antibodies against carbapenemases using a multiplex protein microarray platform.
The study identifies and characterizes monoclonal antibodies targeting various carbapenemases (bla KPC, bla NDM, bla IMP-1, bla VIM, bla OXA-23, bla OXA-48, bla OXA-58) and the mcr-1 gene, which confers resistance to colistin. These antibodies show high specificity and sensitivity in detecting resistance determinants using a protein microarray platform.
High-throughput screening of monoclonal antibodies against carbapenemases using a multiplex protein microarray platform.
The study identifies and characterizes monoclonal antibodies targeting various carbapenemases (bla KPC, bla NDM, bla IMP-1, bla VIM, bla OXA-23, bla OXA-48, bla OXA-58) and the mcr-1 gene, which confers resistance to colistin. These antibodies show high specificity and sensitivity in detecting resistance determinants using a protein microarray platform.
Genomic features and fitness cost of co-existence of bla (KPC-2) and bla (VIM-2) plasmids in ICU-derived pan-drug resistant Pseudomonas aeruginosa.
The study identified the coexistence of bla KPC-2 and bla VIM-2 plasmids in a pan-drug resistant Pseudomonas aeruginosa strain, highlighting their role in carbapenem resistance and stability.
Genomic features and fitness cost of co-existence of bla (KPC-2) and bla (VIM-2) plasmids in ICU-derived pan-drug resistant Pseudomonas aeruginosa.
The study identified the coexistence of bla KPC-2 and bla VIM-2 plasmids in a pan-drug resistant Pseudomonas aeruginosa strain, highlighting their role in carbapenem resistance and stability.
Integrated Sample to Detection of Carbapenem-Resistant Bacteria Extracted from Water Samples Using a Portable Gold Nanoparticle-Based Biosensor.
The study developed a portable biosensor using gold nanoparticles to detect carbapenem-resistant genes bla KPC-3, bla NDM-1, and bla OXA-1 in water samples. The biosensor successfully identified these genes in various water matrices with a limit of detection of 2.5 ng/L.
Towards the integration of antibiotic resistance gene mobility into environmental surveillance and risk assessment.
The study highlights the importance of incorporating antibiotic resistance gene (ARG) mobility into environmental surveillance and risk assessment. It identifies specific ARGs such as bla OXA, bla KPC-2, bla TEM-1, bla GES-2, bla GES-5, sul 1, and sul 2 as highly mobile and potentially risky in various environmental settings.
Coexistence of carbapenem resistance and hypervirulence in clinical Klebsiella pneumoniae isolates: a molecular and phenotypic analysis.
The study identified the presence of carbapenemase genes bla OXA-48, bla NDM, and bla KPC in carbapenem-resistant Klebsiella pneumoniae isolates, highlighting the coexistence of carbapenem resistance and hypervirulence in clinical isolates.
An NGS-assisted diagnostic workflow for culture-independent detection of bloodstream pathogens and prediction of antimicrobial resistances in sepsis.
The study evaluated the diagnostic performance of PISTE™ technology, an NGS-based workflow for detecting bloodstream pathogens and predicting antimicrobial resistance. It showed high accuracy in identifying pathogens and predicting resistance genes, including beta-lactamases, carbapenemases, aminoglycoside modifying enzymes, tetracycline efflux pumps, and quinolone resistance proteins.
Evaluation of expert rules for carbapenemase class identification in Enterobacterales isolates using the VITEK2 susceptibility testing platform.
The study evaluated the performance of bioMérieux Advanced Reporting Tool (bioART) expert rules for identifying carbapenemase classes in Enterobacterales isolates using the VITEK2 platform. It found that the bioART rules, when combined with the VITEK2 Advanced Expert System (AES), improved the detection of carbapenemase-producing isolates, particularly for KPC, MBL, and OXA-48-like enzymes.
Rapid replacement of blaKPC variant in ST11 carbapenem-resistant and hypervirulent Klebsiella pneumoniae contributed to ceftazidime/avibactam resistance during severe in vivo infection.
The study identifies blaKPC-144 as a novel variant that confers resistance to ceftazidime/avibactam in ST11 carbapenem-resistant and hypervirulent Klebsiella pneumoniae, demonstrating its rapid replacement of blaKPC-2 during in vivo evolution.
Klebsiella pneumoniae with Two Carbapenemases: Where Molecular Research Stands Now.
The paper discusses the prevalence and genetic environments of various carbapenemases, including KPC-2, NDM-5, IMP-4, NDM-1, and OXA-48, in Klebsiella pneumoniae, highlighting their roles in multidrug resistance and the importance of plasmid-mediated transmission.
Navigating an evolving microbial landscape: emerging antimicrobial resistance trends and precision stewardship in Tianjin tertiary hospitals (2021-2023).
The study identified significant trends in antimicrobial resistance (AMR) patterns among clinical isolates from hospitals in Tianjin, highlighting the increasing resistance of Klebsiella pneumoniae to various antibiotics, including carbapenems, and the notable decline in ceftazidime/avibactam resistance in E. coli. Additionally, it noted the emergence of resistance in Acinetobacter baumannii and Pseudomonas aeruginosa to several antimicrobials.
Molecular Epidemiology of tet(A)-v1-Positive Carbapenem-Resistant Klebsiella pneumoniae in Pediatric Patients in a Chinese Hospital.
The study identifies the tet(A)-v1 gene as a significant factor in tigecycline resistance among carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates from pediatric patients. It also characterizes various carbapenemase genes, including bla IMP-4, bla NDM-5, bla NDM-1, and bla KPC-2, contributing to carbapenem resistance.
Epidemiological and Microbiological Characterization of Carbapenemase-Producing Klebsiella pneumoniae Isolates in a Regional Greek Hospital: A Retrospective Study.
The study identified multiple carbapenemase-producing Klebsiella pneumoniae isolates, including blaNDM-1, blaVIM-1, blaKPC-2, and blaCTX-M-15, highlighting the prevalence of multidrug-resistant strains in a regional Greek hospital.
Antibiotic resistance in white stork cloaca and environmental samples
The study identified various antibiotic resistance genes in bacterial isolates from white stork cloaca and environmental samples, highlighting the presence of resistance mechanisms against beta-lactams, aminoglycosides, quinolones, and polymyxins.
The carbapenem inoculum effect provides insights into the molecular mechanisms underlying carbapenem resistance in the Enterobacterales.
The study identified that various carbapenemase genes, including blaKPC-3, blaSME-2, blaIMP-4, blaNDM-1, blaVIM-27, blaCMY-10, and blaOXA-48, when expressed in E. coli, significantly increased meropenem MICs and exhibited an inoculum effect. These findings highlight the role of carbapenemases in carbapenem resistance and the importance of the inoculum effect in diagnosing carbapenemase-producing CRE.
In vitro activity of cefiderocol against nosocomial Acinetobacter baumannii.
The study assessed the in vitro activity of cefiderocol against multidrug-resistant Acinetobacter baumannii isolates and found that cefiderocol exhibited high efficacy, with very low resistance rates. However, the presence of various beta-lactamase genes, including bla OXA-23G, bla OXA-24G, bla OXA-48G, bla OXA-58G, bla VEB, bla PER, bla GES, bla KPC, bla NDM, bla VIM, bla IMP, and bla IMI, was associated with resistance to other antibiotics.
Emergence of ST11 Klebsiella pneumoniae co-carrying bla(KPC-2) and bla(IMP-8) on conjugative plasmids.
The study identifies a clinical ST11 Klebsiella pneumoniae strain co-carrying bla(KPC-2) and bla(IMP-8) on conjugative plasmids, highlighting the potential for horizontal gene transfer and multidrug-resistant dissemination.
Clinical, microbiological characteristics and outcomes of carbapenemase-producing enterobacterales bloodstream infections in a pediatric hospital in Brazil.
The study identified KPC and NDM carbapenemases as the主要 resistance mechanisms in carbapenemase-producing Enterobacterales (CPE) bloodstream infections in a pediatric hospital in Brazil.
Emergence and dissemination of bla(KPC-31) and bla(PAC-2) among different species of Enterobacterales in Colombia: a new challenge for the microbiological laboratories.
The study identifies blaKPC-31, blaKPC-33, blaKPC-8, and blaPAC-2 as key genes responsible for ceftazidime/avibactam resistance in Enterobacterales isolates in Colombia, highlighting the emergence of these resistance mechanisms and their potential for dissemination.
Emergence and dissemination of bla(KPC-31) and bla(PAC-2) among different species of Enterobacterales in Colombia: a new challenge for the microbiological laboratories.
The study identifies blaKPC-31, blaKPC-33, blaKPC-8, and blaPAC-2 as key genes responsible for ceftazidime/avibactam resistance in Enterobacterales isolates in Colombia, highlighting the emergence of these resistance mechanisms and their potential for dissemination.
Emergence and dissemination of bla(KPC-31) and bla(PAC-2) among different species of Enterobacterales in Colombia: a new challenge for the microbiological laboratories.
The study identifies blaKPC-31, blaKPC-33, blaKPC-8, and blaPAC-2 as key genes responsible for ceftazidime/avibactam resistance in Enterobacterales isolates in Colombia, highlighting the emergence of these resistance mechanisms and their potential for dissemination.
Bio-assessments of the antibiofilm, antioxidant, and plasmid inhibitory effects of zinc selenide nanoparticles synthesized using Stachys lavandulifolia extract.
The study identified the presence of resistance genes blaKPC, NDM-1, and mecA in multidrug-resistant pathogens, demonstrating the effectiveness of ZnSe nanoparticles in inhibiting these resistance mechanisms.
Geographic containment and virulence-resistance trade-offs drive the evolution of hypervirulent Klebsiella pneumoniae.
The study identifies bla KPC-2, bla NDM-1, bla NDM-5, and bla OXA-48 as key carbapenemase genes in hypervirulent Klebsiella pneumoniae, highlighting their role in resistance and the trade-off with virulence.
Rapid detection of gram-negative antimicrobial resistance determinants directly from positive blood culture broths using a multiplex PCR system.
The study evaluated the BIOFIRE FILMARRAY Antimicrobial Resistance (AMR) Panel for rapid detection of AMR genes in gram-negative bacteria from positive blood culture broths. It identified several AMR genes, including beta-lactamases (TEM-1, SHV-1, CTX-M, KPC, NDM, OXA-1, OXA-23), AmpC beta-lactamases (CMY/MOX, DHA), aminoglycoside modifying enzymes (AAC(6′)-Ib-cr), and fluoroquinolone resistance mutations (ParC-S80I, GyrA-S83F/I/L).
Whole-genome sequencing reveals resistance mechanisms and molecular epidemiology of carbapenem-resistant Pseudomonas aeruginosa bloodstream infections.
The study identified oprD mutations and efflux pumps hyperexpression as dominant drivers of carbapenem resistance in CRPA strains, with carbapenemase emerging as a clinically significant pathway.
Whole-genome sequencing reveals resistance mechanisms and molecular epidemiology of carbapenem-resistant Pseudomonas aeruginosa bloodstream infections.
The study identified oprD mutations and efflux pumps hyperexpression as dominant drivers of carbapenem resistance in CRPA strains, with carbapenemase emerging as a clinically significant pathway.
Mechanistic insights into the formation and spread of carbapenem-resistant hypervirulent Klebsiella pneumoniae via an IncHI1B/repB hybrid virulence plasmid.
The study identifies a novel conjugative IncHI1B/repB virulence plasmid (pHvKP51-Vir) that fuses with a KPC-type resistance plasmid (pKPC) to form a hybrid plasmid (pJ53-KPC_Vir), which confers both carbapenem resistance and hypervirulence in Klebsiella pneumoniae.
Mechanistic insights into the formation and spread of carbapenem-resistant hypervirulent Klebsiella pneumoniae via an IncHI1B/repB hybrid virulence plasmid.
The study identifies a novel conjugative IncHI1B/repB virulence plasmid (pHvKP51-Vir) that fuses with a KPC-type resistance plasmid (pKPC) to form a hybrid plasmid (pJ53-KPC_Vir), which confers both carbapenem resistance and hypervirulence in Klebsiella pneumoniae.
Unravelling tandem repeat-mediated mutagenesis drive rapid diversification of KPC enzymes: emergence of bla(KPC-263) and enhanced resistance to ceftazidime-avibactam.
The study identifies two novel KPC variants, bla(KPC-263) and bla(KPC-90), which emerged through tandem repeat-mediated mutagenesis and confer enhanced resistance to ceftazidime-avibactam.
Unravelling tandem repeat-mediated mutagenesis drive rapid diversification of KPC enzymes: emergence of bla(KPC-263) and enhanced resistance to ceftazidime-avibactam.
The study identifies two novel KPC variants, bla(KPC-263) and bla(KPC-90), which emerged through tandem repeat-mediated mutagenesis and confer enhanced resistance to ceftazidime-avibactam.
Unravelling tandem repeat-mediated mutagenesis drive rapid diversification of KPC enzymes: emergence of bla(KPC-263) and enhanced resistance to ceftazidime-avibactam.
The study identifies two novel KPC variants, bla(KPC-263) and bla(KPC-90), which emerged through tandem repeat-mediated mutagenesis and confer enhanced resistance to ceftazidime-avibactam.
Unravelling tandem repeat-mediated mutagenesis drive rapid diversification of KPC enzymes: emergence of bla(KPC-263) and enhanced resistance to ceftazidime-avibactam.
The study identifies two novel KPC variants, bla(KPC-263) and bla(KPC-90), which emerged through tandem repeat-mediated mutagenesis and confer enhanced resistance to ceftazidime-avibactam.
A beta-lactamase inhibitory protein mutant displays high potency and a broad inhibition profile due to an altered binding mode with beta-lactamases.
The BLIP E73W mutant exhibits high potency and a broad inhibition profile against class A beta-lactamases, achieved through a distinct binding mode that allows it to inhibit multiple beta-lactamase variants effectively.
Antimicrobial resistance among agents of hospital-acquired lower respiratory tract infection in the UK and Ireland: trends from 2008/2009 to 2018/2019.
The study identifies bla OXA-23 and bla OXA-58 as key carbapenem resistance genes in Acinetobacter baumannii and related species, highlighting their role in multidrug resistance.
Characterization of Carbapenem-Resistant Gram-Negative Bacilli Isolates in Multispecialty Private Hospitals in Lagos, Nigeria.
The study identified bla NDM and bla KPC genes in carbapenem-resistant Gram-negative bacilli, highlighting the prevalence of carbapenemase-producing organisms in Lagos, Nigeria.
Molecular epidemiology of carbapenem-resistant Escherichia coli in a tertiary hospital located in the Dabie Mountains region, China.
The study identifies blaNDM-5 as the primary carbapenem resistance gene in CREc isolates, alongside other resistance genes such as blaNDM-13, blaNDM-6, blaKPC-2, and fosA3. These genes contribute to multidrug resistance and are associated with various virulence factors.
Correlation between antimicrobial resistance and virulence in carbapenem-resistant Klebsiella pneumoniae at colonization and infection stages.
All 72 isolates carried the blaKPC-2 resistance gene, indicating stable resistance during colonization-infection transitions. Virulence gene dynamics were observed, with some strains showing increased or decreased virulence gene expression.
Multidrug-resistant Pseudomonas aeruginosa: Pathogenesis, resistance mechanisms, and novel therapeutic strategies.
The paper discusses the multidrug resistance mechanisms of Pseudomonas aeruginosa, including beta-lactamases, aminoglycoside modifying enzymes, efflux pumps, and mutations in porin genes. It highlights the role of these mechanisms in antibiotic resistance and the challenges they pose in treating infections.
Resistome profiling and bacterial community structure of semi-urban gutter ecosystems of India.
The study identified the presence of beta-lactamase genes, specifically blaKPC, in gutter microbiomes, contributing to resistance against penicillins and cephalosporins. High levels of antibiotic-resistant bacteria were found in certain gutter samples, highlighting the potential environmental spread of antimicrobial resistance.
Genomic diversity, antimicrobial resistance and dissemination of Serratia marcescens complex in patients admitted to ICUs.
The study identified multiple AMR genes in Serratia marcescens complex isolates, including beta-lactamases (bla_SRT, bla_CTX-M-14, bla_CTX-M-3, bla_CTX-M-15), carbapenemases (bla_KPC-2, bla_NDM-5), and aminoglycoside-modifying enzymes (aac(6')_Serra). These genes were associated with resistance to various antibiotics, including cephalosporins, carbapenems, and aminoglycosides.
Emergence of co-resistance to imipenem/relebactam and ceftazidime/avibactam in clinical Klebsiella pneumoniae ST11 clone due to KPC-2 N132S and CTX-M-65 S130G/P167S substitutions.
The study identifies the emergence of co-resistance to imipenem/relebactam and ceftazidime/avibactam in clinical Klebsiella pneumoniae ST11 clones due to KPC-2 N132S and CTX-M-65 S130G/P167S substitutions.
Revisiting the Metallo-beta-lactamase-Mediated Antibiotic Resistance: Exploring Novel Mechanisms and Therapeutic Strategies.
The paper discusses the role of metallo-beta-lactamases (MβLs) such as NDM, VIM, and IMP in mediating resistance to carbapenems and other β-lactam antibiotics. It highlights the importance of these enzymes in clinical settings and explores potential therapeutic strategies to combat their resistance mechanisms.
Antibiotic resistance in mastitis-causing bacteria: Exploring antibiotic-resistance genes, underlying mechanisms, and their implications for dairy animal and public health.
The study identifies several AMR genes and mutations in Staphylococcus aureus and coagulase-negative staphylococci associated with mastitis, including blaZ, mecA, tetK, tetM, aphA3, aacA-aphD, aadD, ermA, msrA, mphC, lnuB, and vanA, which confer resistance to various antibiotics such as β-lactams, tetracyclines, aminoglycosides, macrolides, and glycopeptides.
Global genomic insights into the antimicrobial resistance of Escherichia marmotae.
The study identifies bla KPC-2 and bla CTX-M-14 as significant antimicrobial resistance genes in Escherichia marmotae, highlighting its potential as a reservoir for resistance determinants.
Genomic characterization of Klebsiella pneumoniae clinical isolates from cancer patients: resistance profiles, virulence factors, and sequence typing.
The study identified several AMR genes in K. pneumoniae isolates from cancer patients, including genes conferring resistance to trimethoprim-sulfamethoxazole, tobramycin, levofloxacin, and carbapenems. Key resistance genes included sul1, sul2, dfrA1, dfrA12, dfrA14, dfrA27, aadA16, aadA2, aph(3')-Ia, aph(3'')-Ib, aph(6)-Id, aph(4)-Ia, ACC(3)-IId, ACC(3)-IIV, AAC(6')-Ib-cr, QnrS1, QnrB17, QnrB20, QnrB4, CTX-M, SHV, TEM, DHA-1, LAP-2, bla_kpc-1, bla_ndm-5, and bla_oxa-10.
Virulence and Genomic Profiles of Klebsiella pneumoniae Isolated from Pediatric Patients in Henan, China (2021-2023).
The study identified blaKPC-2 as the predominant carbapenemase in pediatric CRKP isolates, along with blaNDM-1 and blaIMP-4. It also found a high prevalence of hypervirulence-associated genes iuc and rmpA2 in CRKP isolates.
Comparative assessment of annotation tools reveals critical antimicrobial resistance knowledge gaps in Klebsiella pneumoniae.
The study identifies various AMR genes and mutations in Klebsiella pneumoniae, highlighting knowledge gaps in existing annotation tools and databases for accurate resistance prediction.
New resistance threat in difficult-to-treat resistance Pseudomonas aeruginosa co-producing AFM and KPC carbapenemases: plasmid dynamic transfer and global phylogeography perspective.
The study identifies bla AFM-2 and bla KPC-2 as critical resistance genes in Pseudomonas aeruginosa isolates, highlighting their role in carbapenem resistance and the potential for plasmid-mediated spread in hospital settings.
The emergence and spread of bla (NDM-1), bla(KPC-2), mcr-10 genes, and the tmexCD2-toprJ2 gene cluster in extensively drug-resistant clinical Raoultella ornithinolytica.
The study identifies the coexistence of bla NDM-1, bla KPC-2, mcr-10, and tmexCD2-toprJ2 genes in an extensively drug-resistant Raoultella ornithinolytica strain, highlighting the complex resistance mechanisms and potential for horizontal gene transfer.
Monitoring of Antimicrobial Resistance Genes and Susceptibility Profiles in Bacterial Isolates From Animal-Origin Meat.
The study identified several AMR genes, including bla_KPC, bla_NDM, bla_OXA-48, bla_SPM, bla_VIM, bla_TEM, bla_SHV, bla_CTX-M, and mcr-1, in bacterial isolates from animal-origin meat samples. These genes conferred resistance to various antibiotics, including carbapenems, beta-lactams, and polymyxins.
Active screening and molecular epidemiological characteristics of fecal colonization by carbapenem-resistant Enterobacterales in intensive care unit wards of a tertiary hospital in Shanghai, China.
The study identified bla KPC-2, bla OXA-1, bla NDM-1, and bla OXA-9 as the主要 carbapenemase genes in CRE-FC strains, with bla KPC-2 being the most prevalent. ST11-KL64 CRKP was found to be the most prevalent fecal colonizer of CRKP and may contribute to hospital outbreaks of infection.
Polyphenols as Potential beta-lactamase Inhibitors: An Integrated Computational and Experimental Study.
This study identifies quercetin, kaempferol, caffeic acid, and 3,4-dihydroxybenzoic acid as potential beta-lactamase inhibitors, demonstrating their ability to inhibit beta-lactamase activity and enhance the efficacy of β-lactam antibiotics against carbapenem-resistant bacteria.
Characterisation of Plasmid-Associated Antimicrobial Resistance Genes in Coastal Marine Enterobacterales from the Central Adriatic Sea: De Novo Assembly and Bioinformatic Profiling.
The study identified nine clinically relevant antimicrobial resistance genes (ARGs) in plasmid-like assemblies from marine Enterobacterales, including blaKPC, blaTEM, aacA4, folP, and tetA, highlighting the potential for horizontal gene transfer in coastal environments.
Detection of K. pneumoniae Hospital-Acquired Strains That Produce Carbapenemases in Thrace Tertiary Hospital.
The study identified the presence of various carbapenemase genes, including blaKPC, blaOXA-48_like, blaVIM, and blaNDM, in K. pneumoniae strains, highlighting the prevalence of these resistance mechanisms in hospital-acquired isolates.
Diversity in Carbapenemases in Enterobacterales in Southeastern Austria Before and During the COVID-19 Pandemic.
The study identified various carbapenemase genes, including blaVIM-1, blaNDM-1, blaOXA-48, blaOXA-181, blaOXA-244, blaKPC-2, blaKPC-3, and blaGES, in carbapenem-resistant Enterobacterales isolates from Southeastern Austria. The prevalence of CRE decreased during the COVID-19 pandemic, and dual-carbapenemase-producing isolates were observed for the first time.
Diversity in Carbapenemases in Enterobacterales in Southeastern Austria Before and During the COVID-19 Pandemic.
The study identified various carbapenemase genes, including blaVIM-1, blaNDM-1, blaOXA-48, blaOXA-181, blaOXA-244, blaKPC-2, blaKPC-3, and blaGES, in carbapenem-resistant Enterobacterales isolates from Southeastern Austria. The prevalence of CRE decreased during the COVID-19 pandemic, and dual-carbapenemase-producing isolates were observed for the first time.
Aeromonas Infections in Humans-Antibiotic Resistance and Treatment Options.
The paper discusses the emergence of multidrug-resistant Aeromonas strains, highlighting the presence of various beta-lactamases (blaCphA, blaKPC, blaNDM, blaVIM), polymyxin resistance genes (mcr-3, mcr-7), and fluoroquinolone resistance (qnrA).
Emergence of mutH (V76G) among longitudinal carbapenem resistant Klebsiella pneumoniae causing long-term colonization and recurrent infection disrupts DNA mismatch repair and results in a hypermutator phenotype.
The study identifies a hypermutator phenotype in K. pneumoniae due to a V76G substitution in the mutH gene, which disrupts DNA mismatch repair and leads to increased resistance to ceftazidime-avibactam and meropenem-vaborbactam.
Genomics, population dynamics, immune evasion and resistance determinants foster the competence and global dissemination of Klebsiella pneumoniae.
The paper identifies several beta-lactamase genes, including blaKPC, blaNDM-1, blaOXA-48, blaCTX-M-15, blaSHV-11, blaKPC-2, blaKPC-3, blaOXA-9, blaOXA-48, blaOXA-181, blaOXA-232, and blaNDM, which confer resistance to carbapenems and other antibiotics in Klebsiella pneumoniae.
Genomics, population dynamics, immune evasion and resistance determinants foster the competence and global dissemination of Klebsiella pneumoniae.
The paper identifies several beta-lactamase genes, including blaKPC, blaNDM-1, blaOXA-48, blaCTX-M-15, blaSHV-11, blaKPC-2, blaKPC-3, blaOXA-9, blaOXA-48, blaOXA-181, blaOXA-232, and blaNDM, which confer resistance to carbapenems and other antibiotics in Klebsiella pneumoniae.
Genomics, population dynamics, immune evasion and resistance determinants foster the competence and global dissemination of Klebsiella pneumoniae.
The paper identifies several beta-lactamase genes, including blaKPC, blaNDM-1, blaOXA-48, blaCTX-M-15, blaSHV-11, blaKPC-2, blaKPC-3, blaOXA-9, blaOXA-48, blaOXA-181, blaOXA-232, and blaNDM, which confer resistance to carbapenems and other antibiotics in Klebsiella pneumoniae.
APC24-7, a covalent combination of boronic acid and chelator moieties, restores β-lactam efficiency against metallo-β-lactamase-producers.
APC24-7, a compound combining a boronic acid and a Zn2+-chelator, effectively restores meropenem susceptibility in metallo-beta-lactamase (MBL)-producing bacteria, including those resistant to taniborbactam.
Novel KPC-2 variants and epidemic ST463 clones underlie ceftazidime/avibactam resistance in carbapenem-resistant Pseudomonas aeruginosa.
The study identifies three novel KPC variants (KPC-14, KPC-33, and KPC-86) in carbapenem-resistant Pseudomonas aeruginosa, which confer high-level resistance to ceftazidime/avibactam. These variants are associated with the epidemic ST463 clone and show potential for horizontal transfer.
Novel KPC-2 variants and epidemic ST463 clones underlie ceftazidime/avibactam resistance in carbapenem-resistant Pseudomonas aeruginosa.
The study identifies three novel KPC variants (KPC-14, KPC-33, and KPC-86) in carbapenem-resistant Pseudomonas aeruginosa, which confer high-level resistance to ceftazidime/avibactam. These variants are associated with the epidemic ST463 clone and show potential for horizontal transfer.
Novel KPC-2 variants and epidemic ST463 clones underlie ceftazidime/avibactam resistance in carbapenem-resistant Pseudomonas aeruginosa.
The study identifies three novel KPC variants (KPC-14, KPC-33, and KPC-86) in carbapenem-resistant Pseudomonas aeruginosa, which confer high-level resistance to ceftazidime/avibactam. These variants are associated with the epidemic ST463 clone and show potential for horizontal transfer.
Prevalence and characterization of carbapenem-resistant gram-negative bacteria from poultry in Tamil Nadu, India.
The study identified bla NDM-1, bla KPC-3, and bla OXA-48-like carbapenemase genes in various carbapenem-resistant gram-negative bacteria isolated from chicken meat samples in Tamil Nadu, India. These genes were found to confer resistance to meropenem.
PanARGMiner (Pan-Genomic Antimicrobial Resistance Gene Miner): An advanced feature selection framework for extracting key resistance genes from pan-genomic datasets.
PanARGMiner effectively identifies key resistance genes from pan-genomic datasets, including both known and novel AMR genes, across multiple bacterial species.
PanARGMiner (Pan-Genomic Antimicrobial Resistance Gene Miner): An advanced feature selection framework for extracting key resistance genes from pan-genomic datasets.
PanARGMiner effectively identifies key resistance genes from pan-genomic datasets, including both known and novel AMR genes, across multiple bacterial species.
A quantitative characterization of antibiotic resistance and its influencing factors in hospital wastewaters across Lebanon.
The study identifies multiple antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) in hospital wastewaters across Lebanon, highlighting the prevalence of resistance to beta-lactams, aminoglycosides, tetracyclines, sulfonamides, and glycopeptides.
Antimicrobial resistance, virulence defects, and evolutionary dynamics of multidrug-resistant Klebsiella pneumoniae from human and animal hosts in Central China.
The study identifies multiple AMR genes and mutations in multidrug-resistant Klebsiella pneumoniae isolates from human and animal hosts in Central China, highlighting the role of plasmid-borne resistance genes and genetic variations affecting virulence.
Molecular characterization of a clinical ST145 Klebsiella oxytoca strain co-producing KPC-2 and IMP-96 carbapenemases.
The study identified a clinical ST145 Klebsiella oxytoca strain co-producing KPC-2 and IMP-96 carbapenemases, which exhibit high-level resistance to carbapenems and ceftazidime-avibactam. Both resistance genes are located on conjugative plasmids, facilitating the spread of antimicrobial resistance.
First report of an Escherichia coli ST131 clinical isolate co-harboring bla(KPC-2) and bla(NDM-13) on an IncB/O/K/Z plasmid in China.
The study reports the first case of an Escherichia coli ST131 isolate co-harboring bla(KPC-2) and bla(NDM-13) on an IncB/O/K/Z plasmid in China, highlighting the emergence of multidrug-resistant plasmids with high transferability and stability.
A mechanism-based pharmacokinetic/pharmacodynamic analysis of polymyxin B-based combination therapy against carbapenem-resistant Klebsiella pneumoniae isolates with diverse phenotypic and genotypic resistance mechanisms.
The study identified bla KPC-2 and bla NDM-4 as resistance genes in carbapenem-resistant Klebsiella pneumoniae isolates. Mutations in mgrB were found to confer resistance to polymyxin B.
Antimicrobial Resistance Profiles of Pseudomonas aeruginosa in a Korean Community Hospital: In Vitro Activity of Ceftazidime-Avibactam and Other Agents.
The study identified IMP- and KPC-type carbapenemase genes in 14.8% of carbapenem-resistant P. aeruginosa isolates, contributing to resistance against carbapenems.
Analysis of the Concordance Between the Use of Phenotypic Screening Tests with the β-Lactamase Gene Profile in Selected Gram-Negative Bacteria.
The study identified several β-lactamase genes, including NDM, KPC, OXA-48, TEM, SHV, CTX-M-15, OXA-24/40, OXA-51, CMY-2, and DHA, which are associated with resistance to carbapenems and β-lactam antibiotics in Gram-negative bacteria.
Elucidation of population-based bacterial adaptation to antimicrobial treatment by single-cell sequencing analysis of the gut microbiome of a hospital patient.
The study identified 29 ARG subtypes across eight types in 13 known, five unknown, and 18 unclassified species, highlighting the complex and dynamic nature of antimicrobial resistance in the gut microbiome. Notably, the cfr(C) gene was detected in 11 bacterial species following antimicrobial treatment, with mutation patterns characterized in several species.
Emergence of KPC-2 and NDM-13-coproducing carbapenem-resistant hypervirulent Klebsiella pneumoniae with high-risk sequence type ST11.
The study identifies a clinical strain of ST11-K64 CR-hvKP co-producing KPC-2 and NDM-13, which carries multiple resistance genes on two plasmids, leading to near-pan-drug resistance.
In-host co-colonization and bloodstream infection by distinct classical and hypervirulent CRKP clones harboring a homologous bla (KPC-2)-harboring plasmid.
The study identifies two distinct CRKP clones, ST268 and ST4496, both harboring the blaKPC-2 gene, which confers carbapenem resistance. The ST268 clone also carries a pK2044-like plasmid, contributing to its hypervirulent phenotype.
Molecular epidemiology of carbapenem-resistant hypervirulent Klebsiella pneumoniae: risk factors and resistance mechanism of ceftazidime/avibactam in China.
The study identifies blaKPC-2, blaOXA-1, blaCTX-M-15, SHV-182, ompK35, and ompK36 as key resistance genes in carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) isolates in China, highlighting their role in resistance to carbapenems and other antibiotics.
Genetic diversity and occurrence of antibiotic resistance genes from community wastewater in Dhaka and Cox's Bazar, Bangladesh.
The study identified nine clinically important antibiotic resistance genes (ARGs) in wastewater samples from Dhaka and Cox's Bazar, Bangladesh. The most prevalent ARGs were blaTEM, blaCTX-M, blaOXA, tolC, and acrA, with blaKPC and blaNDM also being frequently detected. Mutational analysis of KPC revealed unique missense mutations that may alter binding affinity to β-lactam antibiotics.
Cross-regional dynamics of antibiotic resistance (AR): Genomic evidence of transmission for Escherichia coli carrying last-resort AR gene between food animals and humans in China.
The study identifies the transmission of LARG-harboring E. coli between food animals and humans in China, highlighting the role of cross-regional transmission in the spread of antibiotic resistance.
Insights into Klebsiella pneumoniae carbapenem resistance-a two-year retrospective study from a Romanian tertiary care hospital.
The study identifies blaNDM, blaOXA-48, and blaKPC as the primary carbapenemase genes responsible for carbapenem resistance in Klebsiella pneumoniae isolates from a Romanian tertiary care hospital.
Molecular resistance mechanisms to newly approved antibiotics (2017-2025) in WHO priority pathogens.
The paper reviews molecular resistance mechanisms to newly approved antibiotics in WHO priority pathogens, identifying various beta-lactamases, efflux pumps, and target site modifications that confer resistance.
Real-world usage, effectiveness, and microbiological features of ceftazidime-avibactam in clinical practice in China.
The study identified blaKPC genes in carbapenem-resistant Klebsiella pneumoniae isolates, which confer resistance to carbapenems.
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