The study reveals significant transitions in the antibiotic resistome during municipal wastewater treatment, highlighting the presence of various ARGs in untreated wastewater and their reduced abundance in treated samples. Notably, certain ARGs like blaOXA, mef(A), and msr(D) were prevalent in untreated wastewater, while others like aph(3")-Ib, aph(6)-Id, mef(C), mph(A), and mph(G) were more prominent in activated sludge samples.