The study identified multiple antimicrobial resistance genes in Vibrio cholerae O1 isolates from Ghana, including strA, strB, catB5, floR, sul2, and dfrA1, which confer resistance to streptomycin, chloramphenicol, florfenicol, sulfonamides, and trimethoprim/sulfamethoxazole. Additionally, mutations in gyrA (S83I) and parC (S85L) were found to contribute to fluoroquinolone resistance.