dnaA

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DNA replication initiator protein

NCBI Pathogen Isolates

Bacterial genomes carrying this gene

NCBI Reference Gene Catalog

Known alleles and reference sequences

UniProt

Protein entries specific to dnaA

AMR Rules

Browse AMR rules for dnaA

Validation StatusCandidate

Overview

Mechanism: mutation in the gene leads to resistance to streptomycin
Validation Method: machine learning and structural analysis
Drug Classes: streptomycin, isoniazid
Organisms: Mycobacterium tuberculosis
Geographic Location: Belarus|South Africa|South Korea|India, China|Vietnam|Peru|Netherlands
Papers: 2
Databases: Reslit
Mutations: 3

Mutations

Protein Change	Nucleotide Change	Mechanism	Organism	Resistance To	Database	Validation Status
I282T	T845C	-	-	isoniazid	Reslit	Candidate
R400H	C1200T	-	-	isoniazid	Reslit	Candidate
M484I	G1452A	-	-	isoniazid	Reslit	Candidate

Papers2

Machine learning and structural analysis of Mycobacterium tuberculosis pan-genome identifies genetic signatures of antibiotic resistance.

PMID 30333483 · 2018

The study identifies 33 known AMR genes and 24 new genetic signatures of AMR in Mycobacterium tuberculosis using machine learning and structural analysis. It also reveals 97 epistatic interactions across 10 resistance classes and provides mechanistic insights into the selection of these genes.

Gene Information

Gene Name: dnaA
Allele: DnaA
Encodes: DNA replication initiator protein
Source Database: Reslit
Mechanism: mutation in the gene leads to resistance to streptomycin
Confers Resistance To: streptomycin
Organisms Tested In: • Mycobacterium tuberculosis
Geographic Location: Belarus|South Africa|South Korea|India
Validation Method: machine learning and structural analysis

Mutations in dnaA and a cryptic interaction site increase drug resistance in Mycobacterium tuberculosis.

PMID 33253310 · 2020

Non-synonymous mutations in dnaA are associated with drug resistance in clinical M. tuberculosis strains, particularly isoniazid resistance. These mutations lead to decreased katG expression, which contributes to reduced susceptibility to isoniazid.

Gene Information

Gene Name: dnaA
Allele: DnaA
Encodes: essential and highly conserved regulator of DNA replication
Source Database: Reslit
Mechanism: non-synonymous mutations in dnaA are associated with drug resistance in clinical M. tuberculosis strains
Confers Resistance To: isoniazid
Organisms Tested In: • Mycobacterium tuberculosis
Geographic Location: China|Vietnam|Peru|Netherlands
Validation Method: genomic associations, isogenic strain construction, competition assays, transcriptional profiling
Sequence Accession: PRJNA355614|PRJNA268900