The study characterizes the role of mecA in oxacillin resistance in Staphylococcus epidermidis and identifies a mutation in mecI that reduces its repressive effect on mecA transcription, leading to increased resistance.

The study evaluated the MRSA-Screen latex agglutination test for detecting mecA-positive coagulase-negative staphylococci, demonstrating high sensitivity and specificity for methicillin resistance.

The study characterizes the genetic organization of the mecA element in methicillin-resistant Staphylococcus aureus isolates, focusing on the downstream region and identifying variations in the genetic structure associated with different polymorphisms of the mecA gene.

The study describes a rapid PCR-based method for detecting the mecA gene, which is responsible for methicillin resistance in Staphylococcus aureus and coagulase-negative staphylococci.

The study identifies the role of IS431-mediated deletion of mecI in the expression of methicillin resistance in mecA-carrying, low-level methicillin-resistant Staphylococcus haemolyticus. The deletion of mecI leads to derepression of mecA, resulting in increased methicillin resistance.

The paper discusses molecular detection methods for antimicrobial resistance, focusing on genes like mecA and blaZ, which confer resistance to beta-lactam antibiotics in staphylococci.

The study analyzed the mecA gene, which encodes PBP 2a, and its role in methicillin resistance in Staphylococcus aureus. It found that mecA-positive strains can exhibit phenotypic susceptibility to methicillin under certain conditions, highlighting the importance of molecular detection methods like PCR for accurate identification of MRSA.

The study describes a multiplex PCR assay for the detection of methicillin and mupirocin resistance genes in Staphylococcus aureus, enabling rapid and specific identification of resistant isolates.

The study identifies a methicillin-resistant Staphylococcus aureus (MRSA) strain responsible for a foodborne outbreak, highlighting the potential for community-acquired MRSA to cause gastroenteritis. The MRSA strain was resistant to penicillin and oxacillin but susceptible to other antibiotics.

The study shows that oxacillin-resistant staphylococci can transition from a heterotypic to a homotypic resistance phenotype under certain growth conditions, and this conversion is dependent on the mecA gene and its transcriptional regulation.

The study describes a rapid PCR-based method for identifying methicillin-resistant Staphylococcus aureus (MRSA) using the mecA and femB genes, which are critical for methicillin resistance.

The study identifies the mecA gene as responsible for methicillin resistance in community-acquired MRSA strains in Finland, highlighting the horizontal acquisition of the mecA gene in previously susceptible S. aureus strains.

The study developed a molecular assay for the rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) by targeting the S. aureus -specific gene and the mecA gene, which encodes the PBP2a protein responsible for methicillin resistance.

The study evaluates techniques for detecting low-level methicillin-resistant Staphylococcus aureus (MRSA), identifying the mecA gene as a key factor in methicillin resistance through PCR analysis.

The study identifies the mecA gene in Staphylococcus sciuri as a native gene that can confer methicillin resistance upon transcriptional activation. Mutations in the promoter region and insertion of IS 256 element were found to enhance mecA expression, leading to methicillin resistance.

The study describes a novel molecular assay for the rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) directly from clinical samples. The assay uses a triplex qPCR targeting the mecA gene, which confers methicillin resistance, along with S. aureus and S. epidermidis specific femA genes to distinguish MRSA from other staphylococcal species.

The study reports the first case of a Staphylococcus lugdunensis infection carrying the mecA gene, which confers resistance to methicillin and oxacillin.

The study characterizes mutations in the mecI gene that affect the repression of mecA, leading to increased oxacillin resistance in Staphylococcus aureus clinical isolates.

The study evaluated the oxacillin susceptibilities of 152 coagulase-negative staphylococcal (CoNS) strains and found that the mecA gene is responsible for oxacillin resistance. The agar screening test with 4 μg of oxacillin per ml and incubation for 48 h was highly effective in detecting oxacillin resistance.

The study identifies mecA as a gene that confers beta-lactam resistance in Staphylococcus aureus by encoding PBP2a, a penicillin-binding protein that is resistant to beta-lactam antibiotics. The research shows that the expression of mecA is regulated by mecR1-mecI and that the presence of regulatory genes influences the stability and expression of mecA in different genetic backgrounds.

The study describes a multiplex PCR assay for detecting nine clinically relevant antibiotic resistance genes in Staphylococcus aureus, including mecA, aacA-aphD, erm(A), erm(C), tetK, tetM, and vat genes, which confer resistance to methicillin, aminoglycosides, macrolides, lincosamides, streptogramins, tetracyclines, and quinupristin-dalfopristin.

The study identified methicillin (oxacillin)-resistant Staphylococcus aureus (MRSA) strains from food animals, with the mecA gene being responsible for resistance to methicillin and oxacillin.

The study reports the second VRSA isolate in the United States, which contains the vanA gene responsible for vancomycin resistance and other resistance genes such as mecA, erm(A), and aac(6')-aph(2").

The study identifies different variants of the staphylococcal cassette chromosome mec (SCC mec) in methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase-negative staphylococci (MR-CoNS), highlighting the presence of mecA gene and its association with beta-lactam resistance.

The study describes a real-time multiplex PCR assay for the rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) directly from clinical specimens containing a mixture of staphylococci. The assay detects the mecA gene and the S. aureus chromosomal orfX gene, enabling the identification of MRSA strains with high specificity and sensitivity.

The study identifies the mecA gene as responsible for low-level resistance to oxacillin in the epidemic Berlin methicillin-resistant Staphylococcus aureus clone in the Netherlands.

The study developed a quadriplex PCR assay to detect methicillin and mupirocin resistance in Staphylococcus aureus and coagulase-negative staphylococci, targeting the mecA and mupA genes.

The study reports the first known case of human-to-animal transmission of methicillin-resistant Staphylococcus aureus (MRSA) in the Netherlands, highlighting the potential for MRSA to spread between humans and pets.

The study identified seven novel variants of the staphylococcal chromosomal cassette mec (SCC mec) in methicillin-resistant Staphylococcus aureus (MRSA) isolates from Ireland. These variants include SCC mec IIA, IIB, IIC, IID, IIE, IVE, and IVF, which exhibit distinct genomic structures and differences in the mec complex and associated regions.

The study identified the mecA gene in Staphylococcus sciuri isolates, which confers resistance to oxacillin. The presence of mecA was detected in 38.1% of the strains tested.

The study identified two major clonal groups of methicillin-resistant Staphylococcus aureus (MRSA) in Saskatchewan, Canada. Clonal group A was associated with a long-term care facility and exhibited multidrug resistance, while clonal group B was community-associated and showed susceptibility to most antimicrobial agents except for high-level mupirocin resistance. Both groups harbored the mecA gene, which confers resistance to beta-lactam antibiotics.

The study correlated PBP2a detection with oxacillin resistance in Staphylococcus aureus and discovered a novel PBP2a mutation (M483I) in an oxacillin-susceptible isolate, highlighting the complexity of resistance mechanisms.

The study presents a multiplex molecular beacon platform for the rapid detection of methicillin (mecA) and vancomycin (vanA) resistance genes, as well as the lukF gene encoding the Panton-Valentine leucocidin in Staphylococcus aureus.

The study demonstrates that the mecA homologue from Staphylococcus sciuri, when introduced into Staphylococcus aureus, confers high-level beta-lactam resistance through the expression of a transpeptidase domain similar to high-molecular-weight penicillin-binding proteins.

The study characterizes the mecA gene and specific mutations in the quinolone resistance-determining regions (QRDRs) of gyrA, grlA, and grlB genes associated with levofloxacin resistance in Staphylococcus aureus.

The study characterizes a community-associated methicillin-resistant Staphylococcus aureus strain (USA300-0114) that exhibits resistance to multiple antibiotics, including penicillin, oxacillin, erythromycin, tetracycline, and fluoroquinolones. Key resistance genes identified include blaZ, msrA, mecA, tet(K), and ermC, along with the Panton-Valentine leucocidin toxin genes lukF-PV and lukS-PV.

The study identifies methicillin-resistant (mecA-positive) and biofilm-producing (icaAB-positive) Staphylococcus epidermidis strains as contributors to postoperative endophthalmitis.

The study identified the presence of the mecA gene, which confers methicillin resistance, and tetracycline resistance genes tet(M) and tet(K) in Staphylococcus aureus isolates from phase 3 clinical trials of tigecycline.

The study identified the mecA gene in methicillin-resistant staphylococcal isolates from cats and dogs, demonstrating that the gene is identical to that found in human MRSA strains, suggesting potential zoonotic transfer.

The study identified methicillin-resistant Staphylococcus aureus (MRSA) strains in dogs and hospital staff, including the human epidemic strain EMRSA-15. It also found distinct MRSA strains in horses, highlighting potential zoonotic transmission risks.

The study identifies various methicillin-resistant Staphylococcus aureus (MRSA) clones in Western Australia, highlighting the prevalence of SCC mec type IV and V, and the presence of the mecA gene conferring resistance to oxacillin.

The study evaluated methods for detecting oxacillin resistance in the Staphylococcus sciuri group, identifying the mecA gene as a key marker for resistance.

A new methicillin-resistant Staphylococcus aureus clone (ST377) was identified, carrying the mecA gene and SCC mec type V cassette, associated with necrotizing pneumonia.

The study identifies and characterizes bacterial pathogens causing bloodstream infections using a DNA microarray. It highlights the presence of resistance genes such as mecA, blaZ, ermA, aacA-aphD, blaTEM-106, and aacC2, which confer resistance to various antibiotics.

The study identified the presence of mecA gene conferring resistance to oxacillin, methicillin, and cefoxitin in 26.9% of S. aureus isolates, and mupA gene associated with mupirocin resistance in 7% of isolates.

The study identifies new variants of the mecA and mph(C) genes in Staphylococcus species isolated from horses, which confer resistance to beta-lactam and macrolide antibiotics, respectively. Additionally, the aac(6')-Ie-aph(2')-Ia gene was found to confer resistance to gentamicin.

The study identified methicillin-resistant Staphylococcus aureus strains carrying the mecA gene, which confers resistance to methicillin and oxacillin. No vancomycin resistance was detected.

The study evaluated the accuracy of cefoxitin disk testing and oxacillin-salt agar screening (MHOX) for detecting oxacillin resistance mediated by the mecA gene in coagulase-negative staphylococci (CoNS). The mecA gene, encoding penicillin-binding protein 2a, was found to confer oxacillin resistance in various CoNS species.

Two cases of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) were identified in Colombia, with the isolates showing resistance to specific antibiotics and carrying the mecA and msrA genes.

The study identifies a methicillin-resistant Staphylococcus aureus strain (2884D) that exhibits beta-lactam dependency due to constitutive expression of the mecA gene, which encodes penicillin-binding protein 2a. Mutations in regulatory systems (mecI and mecR1) lead to loss of regulation of mecA.

The study identifies the SCCmecN1 element in MRSA isolates from injection drug users, which contains the mecA gene for methicillin resistance, dfrA for trimethoprim resistance, and fusB1 for low-level fusidic acid resistance. Variability in SCCmecN1 was observed, affecting resistance profiles.

The study identified the deletion of the mecA, ant(4′)Ia, and aacA-aphD genes in Staphylococcus aureus isolates leading to the emergence of methicillin-susceptible, gentamicin-resistant and methicillin-resistant, gentamicin-susceptible strains.

The study developed a DNA microarray for the simultaneous detection of antibiotic resistance genes in staphylococcal clinical isolates, identifying genes such as mecA, blaZ, aac(6')-Ie-aph(2''), ermA, ermC, and msrA, which confer resistance to methicillin, penicillin, gentamicin, erythromycin, clindamycin, and macrolides/streptogramin B.

The study evaluated the accuracy of various methods for detecting methicillin resistance in Staphylococcus aureus, highlighting the importance of the mecA gene in mediating resistance and the effectiveness of Etest and oxacillin agar screening plates.

The study identifies the mecA gene as the primary determinant of methicillin resistance in Staphylococcus aureus, located within the SCCmec genomic island. It highlights the importance of the attB site for SCCmec integration and the variability of the attB region among different S. aureus isolates.

The study characterizes new SCCmec elements and mutations in topoisomerase genes associated with fluoroquinolone and methicillin resistance in Staphylococcus pseudintermedius.

The study identified that the mecA gene, which encodes penicillin-binding protein 2A, is responsible for resistance to oxacillin and ceftobiprole in methicillin-resistant Staphylococcus aureus (MRSA) strains. The gene was experimentally validated through purification of PBP 2A and binding assays.

The study identifies the mecA gene as a key determinant of methicillin resistance in Staphylococcus aureus and coagulase-negative staphylococci through PCR detection.

The study identifies a novel SCC mec element in a methicillin-resistant Staphylococcus aureus strain, carrying a class C1 mec complex and a type 5 ccr gene complex, which confers resistance to oxacillin.

The study found that 67.2% of S. aureus isolates were MRSA, with 74.5% of these being mecA positive. mecA-negative BORSA strains were also identified, highlighting the importance of accurate MRSA typing for appropriate antibiotic selection.

The study identified the presence of antibiotic resistance genes including tetO, ermB, mecA, blaSHV-5, ampC, and vanA in reclaimed water from three European artificial groundwater recharge systems. These genes were detected using real-time qPCR, indicating their potential impact on the environment.

The study identifies the mecA gene as a key determinant of methicillin resistance in non-epidermidis coagulase-negative staphylococci, with 71% of isolates showing resistance via mecA gene detection.

The study identifies a novel SCC mec type (type VIII) in a Canadian MRSA strain, characterized by a unique combination of class A mec and type 4 ccr gene complexes. The mecA gene was found to confer resistance to beta-lactam antibiotics.

The study identifies the presence of the mecA gene in Staphylococcus epidermidis strains isolated from breast milk of women with infectious mastitis, which confers resistance to oxacillin.

The study identifies mecA as a gene that confers resistance to oxacillin in methicillin-resistant Staphylococcus aureus (MRSA) and shows that its expression is induced by beta-lactam antibiotics, leading to high-level homotypic resistance.

The study developed a PCR- and microarray-based assay for the rapid identification of bacterial pathogens, including the detection of the methicillin resistance gene mecA in Staphylococcus species.

The study describes a rapid and high-throughput method for molecular characterization of Staphylococcus aureus using PCR coupled with electrospray ionization-mass spectrometry (ESI-MS). The method successfully identified genes associated with antibiotic resistance, including mecA, ermA, ermC, and mupA, which confer resistance to methicillin, erythromycin, and mupirocin.

The study highlights the emergence of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) and characterizes the genetic elements, such as blaZ and mecA, that confer resistance. It discusses the role of genomic sequencing in understanding the evolution and virulence of CA-MRSA.

The study identifies several AMR genes and mutations in the MRSA strain TW20, including mecA for methicillin resistance, qacA for antiseptic resistance, Tn554 and ermA1 for streptomycin and erythromycin resistance, BlaZ for beta-lactam resistance, TetM for tetracycline resistance, and DfrG for trimethoprim resistance. Additionally, a point mutation in GyrA (Ser84Leu) confers quinolone resistance.

The study identifies multiple antimicrobial resistance genes and mutations in MRSA strains causing a skin lesion in a Spanish patient, including mecA, tetK, tetL, tetM, ermA, ermB, ermC, msrA, aph(2')-acc(6'), ant(4')-Ia, aph(3')-III, and dfrK, along with quinolone resistance mutations in grlA and gyrA.

The study highlights the significance of the mecA gene in conferring methicillin and oxacillin resistance in Staphylococcus aureus, emphasizing its role in the emergence of hospital and public health threats in Brazil.

The study identified four mecA-positive Staphylococcus intermedius strains, indicating methicillin resistance, and highlighted the association between methicillin resistance and multidrug resistance in canine isolates.

The study identified three MRSA strains carrying the mecA gene, which confers resistance to oxacillin. These strains were related to USA700 and USA300 clones and exhibited characteristics of community-associated MRSA (CA-MRSA).

The study identifies the mecA gene as a key determinant of oxacillin resistance in a hypervariable Staphylococcus epidermidis strain causing persistent infection in an immunocompromised patient.

The study identifies an unstable methicillin-resistant Staphylococcus aureus strain that rapidly loses the mecA gene upon subculturing, highlighting the importance of gene amplification-based methods like GeneXpert for accurate detection.

The study identifies tetracycline resistance genes tet(M) and tet(K), as well as the methicillin resistance gene mecA in the ST398 MRSA isolate S0385, highlighting its multidrug resistance profile.

The study demonstrates the transfer of the SCC mec element carrying the mecA gene from Staphylococcus epidermidis to methicillin-susceptible Staphylococcus aureus, resulting in methicillin resistance.

The study introduces a PNA-based assay for identifying S. aureus and detecting methicillin resistance via the mecA gene, demonstrating high specificity and sensitivity for rapid pathogen detection and resistance profiling.

The study identifies mecA as a gene that confers beta-lactam resistance in Staphylococcus aureus and shows that tarO is essential for maintaining cell wall integrity and resistance to beta-lactams.

The study characterized a new 35-kb SCC mec element in a Staphylococcus cohnii isolate, carrying a class A mec gene complex and new variants of ccrA and ccrB.

The study identifies and characterizes the presence of antibiotic resistance genes (blaTEM, blaCTX-M9, and mecA) in bacteriophage DNA from environmental samples, demonstrating their potential to confer resistance when transferred to bacterial hosts.

The study identified several antibiotic resistance genes in Staphylococcus aureus isolates from Nigeria, including aacA-aphD, ermA, mecA, tetK, and tetM, which confer resistance to gentamicin, erythromycin, oxacillin, and tetracycline. High levels of resistance to tetracycline and trimethoprim/sulfamethoxazole were observed.

The study identifies the presence of methicillin-resistant Staphylococcus aureus (MRSA) in slaughter pigs in Switzerland, highlighting the emergence of the unusual sequence type ST49. It reports various AMR genes such as mecA, blaZ, tet(M), tet(K), erm(A), erm(C), ant(9)-Ia, str, dfr(G), and vga(A)v, which confer resistance to β-lactams, tetracycline, macrolides, lincosamides, streptogramins B, spectinomycin, streptomycin, trimethoprim, and tiamulin.

The study shows that overexpression of mecI does not significantly alter oxacillin resistance levels in most MRSA strains, indicating that other factors may influence mecA regulation and β-lactam resistance.

The study identified a high prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in the surgical units of Mulago hospital in Kampala, Uganda. It also characterized several AMR genes including mecA, blaZ, and aminoglycoside-modifying enzymes such as aac(6')-Ie-aph(2'')-Ia, aph(3')-IIIa, and ant(4')-Ia.

The study shows that the mecA gene, which confers methicillin resistance, reduces the virulence of healthcare-associated MRSA by interfering with the agr quorum sensing system. The S403A mutation in mecA results in a catalytically inactive PBP2a, affecting the ability of MRSA to respond to extracellular AIP.

The study identified the presence of the mecA gene in 11.3% of Staphylococcus aureus isolates, which is associated with oxacillin resistance.

The study shows that the acquisition of homogeneous oxacillin resistance in Staphylococcus aureus leads to a switch from PNAG-dependent to protein-mediated biofilm formation and attenuates virulence. The mecA gene, encoding PBP2a, plays a central role in this process, and mutations in the gdpP gene, particularly R602H, are linked to the development of homogeneous oxacillin resistance.

The study evaluated the effectiveness of colorimetric methods (nitrate reductase analysis and resazurin microplate assay) for rapid determination of methicillin resistance in Staphylococcus aureus clinical isolates, demonstrating high agreement with the reference microdilution method.

The study identified several antibiotic resistance genes in staphylococcal isolates, including mecA for methicillin resistance, aac(6')/aph(2"), aph(3')-IIIa, and ant(4')-Ia for gentamicin resistance, ermA, ermB, ermC, and msrA for erythromycin resistance, tetK and tetM for tetracycline resistance, and blaZ for penicillin resistance.

The study found that the mecA gene was present in 41.4% of S. aureus isolates and conferred resistance to methicillin, while the PVL gene was present in 24.1% of isolates but was not associated with mecA.

The study identified the presence of mecA, which confers methicillin resistance, and PVL genes (lukF-PV and lukS-PV) in Staphylococcus aureus nasal isolates from medical students in Cartagena, Colombia.

The study identified the presence of the ermC gene in three out of five Staphylococcus lugdunensis isolates, which conferred resistance to erythromycin, clindamycin, and penicillin. Additionally, the mecA gene was found in one isolate, indicating resistance to cefoxitin and penicillin.

The FilmArray BC panel accurately identifies pathogens and detects resistance genes such as mecA, vanA, and blaKPC from positive blood cultures.

The study compared PCR/ESI-TOF-MS with traditional clinical microbiology for detecting organisms and resistance genes in healthcare environments. It identified mecA, vanA, and KPC-3 resistance genes in various samples.

The study identified the mecA gene as responsible for methicillin resistance in Staphylococcus aureus and coagulase-negative staphylococci (CNS). It also found that 72.5% of CNS and 82.3% of S. aureus isolates were resistant to cotrimoxazole. The study highlights the importance of species-level identification of staphylococci for understanding their clinical relevance and resistance patterns.

The study evaluated the LightCycler MRSA advanced test for detecting methicillin-resistant Staphylococcus aureus (MRSA) and found that the mecA gene was responsible for methicillin resistance.

The study identifies a novel 51-kb pseudo SCC mec -SCC-SCC CRISPR composite element in ST779/t878 MRSA isolates, containing mecA, fusC, copB, copC, and novel ccrAB4 and ccrC alleles.

The study identifies a novel MRSA variant carrying the mecA LGA251 gene in companion animals, highlighting the potential zoonotic risk and the need for updated detection methods.

The study reports the isolation of methicillin-resistant Staphylococcus pseudintermedius (MRSP) from wild Norway rats, highlighting the potential for zoonotic transmission and the presence of multiple antimicrobial resistances.

The study identified multidrug-resistant Enterococcus spp., including high-level aminoglycoside-resistant (HLAR) strains, and methicillin-resistant Staphylococcus aureus (MRSA) in dog fecal samples, highlighting the potential public health risks posed by environmental contamination.

The study identified several AMR genes and mutations associated with drug resistance in S. aureus, including blaZ, mecA, tet, tetM, aacA-aphD, and mutations in rpsL, rpsJ, grlA, and gyrA.

The study identifies key AMR genes and mutations in the EMRSA-15 pandemic clone, including blaZ, ermC, mecA, and mutations in grlA and gyrA that confer fluoroquinolone resistance.

The study found that the mecA gene is correlated with methicillin resistance in Staphylococcus epidermidis isolates, with a better correlation observed at a lower MIC breakpoint of 0.5 µg/ml.

The study identified the presence of methicillin-resistant Staphylococcus aureus (MRSA) in retail meat products and human isolates, highlighting the prevalence of mecA gene-mediated resistance.

The study developed a PCR-reverse blot hybridization assay (REBA Sepsis-ID test) for the rapid identification of pathogens in sepsis, including detection of antibiotic resistance genes mecA, vanA, and vanB.

The study reports the second case of methicillin-resistant Staphylococcus pseudintermedius (MRSP) infection in a human host, highlighting the need for molecular techniques for accurate identification and the importance of considering MRSP in immunocompromised patients.

The study characterizes the genetic context of mecA in Staphylococcus haemolyticus isolate WCH1, identifying a 40-kb region containing mecA, which is bracketed by two copies of IS 431 and flanked by an 8-bp direct repeat sequence. The absence of ccr genes suggests alternative mechanisms for mecA mobilization.

The study identified mecA, ermC, and ermB genes associated with methicillin and erythromycin resistance in Staphylococcus aureus isolates from pig tonsils and cow's milk in China.

The study identified mecA, vanA, blaSHV, and blaCTX-M genes in bone marrow transplant patients with bloodstream infections using real-time PCR.

The study identified methicillin resistance in 81% of S. hominis isolates, primarily mediated by the mecA gene. High resistance rates to various antibiotics were observed, highlighting the significance of S. hominis as a pathogen in immunocompromised patients.

The study identifies a novel ST9-SCCmecIX MRSA clone in Thailand, which carries the mecA gene conferring resistance to beta-lactam antibiotics.

The study identified the presence of antibiotic resistance genes mecA and qac in airborne Staphylococcus isolates from metro stations, indicating a higher level of resistance compared to park samples.

The study identifies a single point mutation in gyrB (G431T) that correlates with novobiocin resistance in S. hominis subsp. novobiosepticus. It also characterizes the presence of mecA and ccrAB4 genes associated with oxacillin resistance in this subspecies.

The study identified the mecA gene as the primary determinant of oxacillin resistance in Staphylococcus aureus isolates from neonatal and pediatric units. The mecA gene was detected in 18% of the isolates, and its presence correlated with oxacillin resistance.

The study identified the mecA gene as a key determinant of methicillin resistance in Staphylococcus aureus isolates from surgical site infections. The pvl gene was also detected, indicating the presence of virulent strains.

The study evaluated the Verigene Gram-Positive Blood Culture Test (BC-GP) for the detection of Gram-positive bacteria and resistance determinants directly from positive blood cultures. The test showed high sensitivity and specificity for identifying Staphylococcus aureus, Staphylococcus epidermidis, and Enterococcus species, as well as detecting the mecA gene for methicillin resistance and the vanA gene for vancomycin resistance.

The study characterizes the mecA and mecA_LGA251 genes, which confer methicillin resistance in Staphylococcus aureus isolates. Both genes were experimentally validated through PCR and sequencing.

The study demonstrates the transduction of SCC mec elements, particularly mecA, between Staphylococcus aureus strains, highlighting the role of penicillinase plasmids and the need for extended selection times for mecA expression.

The study identifies genes involved in the metabolic adaptation of MRSA strains under β-lactam pressure, highlighting the role of the TCA cycle and specific genes like mecA, citB, citZ, acsA, pbp2, and femA in mediating resistance to oxacillin.

The study identified several genes associated with clinical outcomes in Staphylococcus aureus bloodstream infections, including mecA, bla, ermC, aadD, sed/sej/ser, and ssl11. These genes were found to be significantly associated with mortality, sepsis, and other clinical endpoints.

The study identifies a novel pseudo-staphylococcal cassette chromosome mec element (ψSCCmec57395) in methicillin-resistant Staphylococcus pseudintermedius CC45, along with various AMR genes such as mecA, blaZ, aac(6')-Ie–aph(2')-Ia, aph(3')-III, ant(6)-Ia, sat4, erm(B), tet(M), dfr(G), and cat pC221. Additionally, fluoroquinolone resistance was linked to specific mutations in gyrA and grlA.

The study identifies the presence of mecA-harboring Staphylococcus isolates in the nasal microbiota of companion animals, highlighting the occurrence of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) in South America.

The study identified mecA as the primary gene responsible for methicillin and oxacillin resistance in Staphylococcus isolates from farm animals in South Africa. No vancomycin resistance genes (vanA, vanB) were detected.

The study identifies mecA and blaZ as genes responsible for methicillin resistance in MRSA, demonstrating their role in conferring resistance to beta-lactam antibiotics.

The study identified the mecA gene in 80% of oxacillin-resistant S. aureus isolates, confirming its role in beta-lactam resistance. Most isolates were susceptible to vancomycin.

The study identified the mecA gene as a major determinant of β-lactam resistance in various staphylococcal species isolated from pigs with exudative epidermitis, including Staphylococcus hyicus, Staphylococcus aureus, and others. The majority of methicillin-resistant isolates carried SCC mec type V.

The study identified the mecA gene as a key determinant of oxacillin resistance in coagulase-negative staphylococci (CoNS) strains isolated from neonatal blood cultures. The mecA gene was detected in 69 out of 100 CoNS strains, with varying prevalence among different species.

The study identified multidrug-resistant strains of Staphylococcus xylosus and Staphylococcus equorum in Slovak Bryndza cheese, with oxacillin resistance primarily mediated by the mecA gene.

The study identifies promoter mutations in the mecA gene that significantly influence oxacillin resistance levels in Staphylococcus aureus by modulating PBP 2a production.

The study identifies three different homologues of the mecA gene, including mecA, mecA1, and mecC, in Staphylococcus sciuri subsp. carnaticus isolates. Both mecA and mecC were found to be expressed under laboratory conditions with low levels of oxacillin, contributing to the resistance phenotype.

The study identified multiple antibiotic resistance genes in coagulase-negative staphylococci (CNS) isolated from very low birth weight neonates, including mecA, ermC, msrA, aac(6')/aph(2''), and aph(3')-IIIa, which conferred resistance to methicillin, erythromycin, clindamycin, gentamicin, and amikacin.

The study identified the presence of methicillin-resistant Staphylococcus aureus (MRSA) and high-level mupirocin-resistant strains in nasal swabs of emergency department patients using PCR/ESI-MS technology.

The study characterizes the SCC composite island in Staphylococcus haemolyticus SH32, identifying the mecA gene conferring methicillin resistance and the ccrA5 and ccrB3 genes involved in SCC element integration.

The study identifies a high prevalence of oxacillin-susceptible mecA-positive Staphylococcus aureus (OS-MRSA) in bovine mastitis cases in China, highlighting the importance of genetic testing alongside phenotypic assays for accurate MRSA identification.

The study identified the presence of methicillin-resistant Staphylococcus aureus (MRSA) and PVL-positive methicillin-sensitive S. aureus (MSSA) in Indonesian hospitals, highlighting the significance of mecA and pvl genes in the epidemiology of these strains.

The study evaluates the use of mecA PCR as an adjunct to routine susceptibility testing for detecting methicillin resistance in coagulase-negative staphylococci, demonstrating high detection rates of the mecA gene among various species.

The study developed a multiplex real-time PCR assay for the rapid detection of methicillin-resistant staphylococci, including MRSA and MRCoNS, directly from positive blood cultures. The assay targets the mecA gene, which confers methicillin resistance.

The study identified the blaZ and mecA genes in ophthalmic Staphylococcus pseudintermedius isolates from dogs, with blaZ conferring resistance to penicillin and mecA conferring resistance to oxacillin and penicillin.

The study identified the presence of methicillin-resistant Staphylococcus aureus (MRSA) in traditional cheeses in the Northwest of Iran, with 21% of S. aureus isolates showing resistance to methicillin via the mecA gene.

The study identified several antibiotic resistance genes, including aac(6')-aph(2"), ermC, msrA, tetK, tetM, and mecA, in faecal isolates of coagulase-negative staphylococci from Nigeria. These genes were associated with resistance to gentamicin, erythromycin, and tetracycline, as well as methicillin resistance.

The study developed a real-time PCR method for differentiating Staphylococcus aureus and coagulase-negative Staphylococcus species, and detecting methicillin resistance through the mecA gene.

The study identified methicillin-resistant coagulase-negative staphylococci (MRCoNS) in milk from cows with mastitis in Brazil, highlighting the presence of various antimicrobial resistance genes including mecA, tet(K), str, ant(4')-I, aac(6')-aph(2"), lnu(B), lsa(E), and a modified erm(C) gene with an 89-bp deletion.