The study identified a diverse array of antimicrobial resistance genes in drinking water stored in cisterns from two First Nations communities in Manitoba, Canada. Key findings include the presence of genes such as aac(3')-Ia, aac(6')-Iia, aac(6')-Iic, aph(3')-Ia, acrD, smeB, smeR, FEZ-1, rm3, SPG-1, OXA-21, OXA-119, OXA-205, dfrA14, dfrB6, acrB, acrF, adeF, ceoB, emrA, mexE, mexF, mexI, oprN, oqxB, BRP(MBL), vanSO, axyY, CRP, efrB, macB, mexB, mexC, mexD, mexK, mexQ, mexW, mexY, mtrA, muxB, muxC, oleB, oleC, ompB, oprM, smeD, smeE, golS, mdsB, PER-2, TEM-126, msbA, arnA, bacA, bcrA, MCR-5, rosA, rosB, rpoB2, ugd, mexN, taeA, efpA, rphA, rphB, otr(A), otrC, tetA(48 ), ompH, and triC, which confer resistance to various antibiotics including aminoglycosides, beta-lactams, cephalosporins, carbapenems, fluoroquinolones, macrolides, monobactams, nitroimidazoles, peptides, phenicols, pleuromutilins, rifamycins, tetracyclines, and triclosan.