mgrB

transmembrane protein conferring colistin resistance

Validation StatusConfirmed2+ DBs

Overview

Mechanism: resistance by absence
Validation Method: PCR and sequencing
Drug Classes: polymyxin b, colistin, polymyxins, ceftriaxone, polymyxin b|colistin, tigecycline, trimethoprim
Organisms: Escherichia coli str. K-12 substr. MG1655, Klebsiella pneumoniae, Escherichia coli, E. coli, Citrobacter werkmanii, Enterobacter cloacae, Staphylococcus aureus, Salmonella enterica
Alleles: 2
Papers: 59
Databases: Card DatabaseReslit
Mutations: 93

Mutations

Protein Change	Nucleotide Change	Mechanism	Organism	Resistance To	Database	Validation Status
C28F	-	PhoP/PhoQ regulatory lipoprotein MgrB	Escherichia coli, Klebsiella pneumoniae	colistinpolymyxinsceftriaxone	ReslitReference Gene Catalog	Candidate
V32G	-	deleterious, PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	ReslitReference Gene Catalog	Candidate
L17R	-	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	ReslitReference Gene Catalog	Candidate
C28Y	-	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistinpolymyxins	ReslitReference Gene Catalog	Candidate
C28W	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
M1V	-	-	Escherichia coli, Klebsiella pneumoniae, Citrobacter werkmanii	colistin	Reslit	Candidate
V8A	-	-	Escherichia coli	colistin	Reslit	Candidate
R256G	-	-	Klebsiella pneumoniae	colistinpolymyxins	Reslit	Candidate
T157P	A469C	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
R248P	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
G53S	-	-	Klebsiella pneumoniae	colistinpolymyxins	Reslit	Candidate
G119T	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
C28G	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
N358Y	-	-	E. coli	colistin	Reslit	Candidate
R36S	-	-	E. coli	colistin	Reslit	Candidate
S43N	-	-	E. coli	colistin	Reslit	Candidate
W20S	-	-	Klebsiella pneumoniae	polymyxin b\|colistinpolymyxin b	Reslit	Candidate
Q22P	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
C16S	-	-	Enterobacter cloacae	colistin	Reslit	Candidate
R114A	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
R128A	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
T21P	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
C39Y	G116A	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	ReslitReference Gene Catalog	Candidate
W47R	-	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	ReslitReference Gene Catalog	Candidate
G37C	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
N25T	-	-	-	polymyxins	Reslit	Candidate
E57G	-	-	-	polymyxins	Reslit	Candidate
T246A	-	-	-	polymyxins	Reslit	Candidate
W20L	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
Q33R	-	-	Escherichia coli	colistin	Reslit	Candidate
T21N	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
W20*	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
V1A	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
L24H	-	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	ReslitReference Gene Catalog	Candidate
K2E	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
K105E	-	inactivation	Klebsiella pneumoniae	colistin	Reslit	Candidate
K3*	-	-	-	polymyxin b	Reslit	Candidate
L4*	-	-	-	polymyxin b	Reslit	Candidate
W6*	-	-	-	polymyxin b	Reslit	Candidate
Q30P	-	-	-	polymyxin b	Reslit	Candidate
S36R	-	-	-	polymyxin b	Reslit	Candidate
F44C	-	-	-	polymyxin b	Reslit	Candidate
P46S	-	-	-	polymyxin b	Reslit	Candidate
G37S	G109A	deleterious, PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	ReslitReference Gene Catalog	Candidate
G164S	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
S257L	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
D86E	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
L322Q	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
Q435H	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
T134P	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
G53V	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
T89P	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
L54H	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
R33Q	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
D31N	-	deleterious, PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	ReslitReference Gene Catalog	Candidate
N42Y	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
K43I	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
W47G	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
G3A	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
A7T	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
G60A	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
K2R	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
K3T	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
C28R	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
M27K	-	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	ReslitReference Gene Catalog	Candidate
A14S	-	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	Reference Gene Catalog	Candidate
W20R	-	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	Reference Gene Catalog	Candidate
Q30Ter	-	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	Reference Gene Catalog	Candidate
F35I	-	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	Reference Gene Catalog	Candidate
C39Ter	-	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	Reference Gene Catalog	Candidate
I45T	-	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	Reference Gene Catalog	Candidate
-	123del	-	Staphylococcus aureus	tigecycline	Reslit	Candidate
-	123ins	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	19del	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	62%fs	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	75ins	-	Klebsiella pneumoniae	polymyxin b\|colistin	Reslit	Candidate
-	7del	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	A315G	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	A41G	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
M1*	A7T	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	A91T	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	A98T	compromises MgrB activity	Escherichia coli	trimethoprim	Reslit	Candidate
-	C64T	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	C88T	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	C96T	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
V37G	G110T	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	G123A	-	Klebsiella pneumoniae, Salmonella enterica	colistin	Reslit	Candidate
Q29*	G29T	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	G405A	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	GAA->TGA	-	-	polymyxins	Reslit	Candidate
-	T-35C	-	Klebsiella pneumoniae	polymyxins	Reslit	Candidate
-	T11A	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
C28S	TGC->AGC	-	Klebsiella pneumoniae	colistin	Reslit	Candidate

Allele Variants2

Allele	Database	Papers	Drug Classes	Organisms	Countries	Years	Sequence Accession	Protein Accession
mgrB	Card Database	1	-	Escherichia coli str. K-12 substr. MG1655	-	-	NC_000913.3	NP_416340.1
mgr B	Reslit	1	polymyxin b	Klebsiella pneumoniae	-	2021	-	-

Papers59

PMID N/A

Gene Information

Gene Name: mgrB
Allele: mgrB
Encodes: transmembrane protein conferring colistin resistance
Source Database: Card Database
Mechanism: resistance by absence
Organisms Tested In: • Escherichia coli str. K-12 substr. MG1655
Sequence Accession: NC_000913.3
Protein Accession: NP_416340.1
Notes: ARO:3003820

In vitro and in vivo antibacterial activity assays of carvacrol: A candidate for development of innovative treatments against KPC-producing Klebsiella pneumoniae.

PMID 33617571 · 2021

The study identified several AMR genes, including bla KPC-2, bla OXA-48, bla NDM-1, bla CTX-M-8, and alterations in the mgr B gene, which contribute to carbapenem and polymyxin resistance in K. pneumoniae strains.

Gene Information

Gene Name: mgrB
Allele: mgr B
Encodes: regulator
Source Database: Reslit
Confers Resistance To: polymyxin b
Organisms Tested In: • Klebsiella pneumoniae
Validation Method: PCR and sequencing

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Mutations

Protein Change	Nucleotide Change	Mechanism	Organism	Resistance To	Database	Validation Status
C28F	-	PhoP/PhoQ regulatory lipoprotein MgrB	Escherichia coli, Klebsiella pneumoniae	colistinpolymyxinsceftriaxone	ReslitReference Gene Catalog	Candidate
V32G	-	deleterious, PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	ReslitReference Gene Catalog	Candidate
L17R	-	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	ReslitReference Gene Catalog	Candidate
C28Y	-	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistinpolymyxins	ReslitReference Gene Catalog	Candidate
C28W	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
M1V	-	-	Escherichia coli, Klebsiella pneumoniae, Citrobacter werkmanii	colistin	Reslit	Candidate
V8A	-	-	Escherichia coli	colistin	Reslit	Candidate
R256G	-	-	Klebsiella pneumoniae	colistinpolymyxins	Reslit	Candidate
T157P	A469C	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
R248P	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
G53S	-	-	Klebsiella pneumoniae	colistinpolymyxins	Reslit	Candidate
G119T	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
C28G	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
N358Y	-	-	E. coli	colistin	Reslit	Candidate
R36S	-	-	E. coli	colistin	Reslit	Candidate
S43N	-	-	E. coli	colistin	Reslit	Candidate
W20S	-	-	Klebsiella pneumoniae	polymyxin b\|colistinpolymyxin b	Reslit	Candidate
Q22P	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
C16S	-	-	Enterobacter cloacae	colistin	Reslit	Candidate
R114A	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
R128A	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
T21P	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
C39Y	G116A	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	ReslitReference Gene Catalog	Candidate
W47R	-	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	ReslitReference Gene Catalog	Candidate
G37C	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
N25T	-	-	-	polymyxins	Reslit	Candidate
E57G	-	-	-	polymyxins	Reslit	Candidate
T246A	-	-	-	polymyxins	Reslit	Candidate
W20L	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
Q33R	-	-	Escherichia coli	colistin	Reslit	Candidate
T21N	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
W20*	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
V1A	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
L24H	-	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	ReslitReference Gene Catalog	Candidate
K2E	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
K105E	-	inactivation	Klebsiella pneumoniae	colistin	Reslit	Candidate
K3*	-	-	-	polymyxin b	Reslit	Candidate
L4*	-	-	-	polymyxin b	Reslit	Candidate
W6*	-	-	-	polymyxin b	Reslit	Candidate
Q30P	-	-	-	polymyxin b	Reslit	Candidate
S36R	-	-	-	polymyxin b	Reslit	Candidate
F44C	-	-	-	polymyxin b	Reslit	Candidate
P46S	-	-	-	polymyxin b	Reslit	Candidate
G37S	G109A	deleterious, PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	ReslitReference Gene Catalog	Candidate
G164S	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
S257L	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
D86E	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
L322Q	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
Q435H	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
T134P	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
G53V	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
T89P	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
L54H	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
R33Q	-	deleterious	Klebsiella pneumoniae	colistin	Reslit	Candidate
D31N	-	deleterious, PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	ReslitReference Gene Catalog	Candidate
N42Y	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
K43I	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
W47G	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
G3A	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
A7T	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
G60A	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
K2R	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
K3T	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
C28R	-	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
M27K	-	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	ReslitReference Gene Catalog	Candidate
A14S	-	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	Reference Gene Catalog	Candidate
W20R	-	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	Reference Gene Catalog	Candidate
Q30Ter	-	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	Reference Gene Catalog	Candidate
F35I	-	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	Reference Gene Catalog	Candidate
C39Ter	-	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	Reference Gene Catalog	Candidate
I45T	-	PhoP/PhoQ regulatory lipoprotein MgrB	Klebsiella pneumoniae	colistin	Reference Gene Catalog	Candidate
-	123del	-	Staphylococcus aureus	tigecycline	Reslit	Candidate
-	123ins	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	19del	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	62%fs	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	75ins	-	Klebsiella pneumoniae	polymyxin b\|colistin	Reslit	Candidate
-	7del	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	A315G	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	A41G	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
M1*	A7T	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	A91T	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	A98T	compromises MgrB activity	Escherichia coli	trimethoprim	Reslit	Candidate
-	C64T	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	C88T	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	C96T	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
V37G	G110T	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	G123A	-	Klebsiella pneumoniae, Salmonella enterica	colistin	Reslit	Candidate
Q29*	G29T	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	G405A	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
-	GAA->TGA	-	-	polymyxins	Reslit	Candidate
-	T-35C	-	Klebsiella pneumoniae	polymyxins	Reslit	Candidate
-	T11A	-	Klebsiella pneumoniae	colistin	Reslit	Candidate
C28S	TGC->AGC	-	Klebsiella pneumoniae	colistin	Reslit	Candidate

Allele

Database

Papers

Drug Classes

Organisms

Countries

Years

Sequence Accession

Protein Accession

mgrB

Card Database

Escherichia coli str. K-12 substr. MG1655

NC_000913.3

NP_416340.1

mgr B

Reslit

polymyxin b

Klebsiella pneumoniae

2021

mgrB

Mutations

Gene Information

Gene Information

Browse AMR Genes

mgrB

Mutations

Gene Information

Gene Information