ResLit - Antimicrobial Resistance Gene & Mutation Database

Geneddl6

D-alanine-D-alanine ligase

Protein ChangeW259C

Nucleotide ChangeG777T

Validation StatusCandidate

Databases

Reslit

Overview

Mechanism: The mutation at c.777 (G>T) leads to a Trp259Cys substitution, which increases the MIC of D-cycloserine by fourfold.
Resistance To: cycloserine
Organisms: Escherichia coli, Treponema denticola
Papers: 1

Paper Information1

Integron gene cassettes harboring novel variants of D-alanine-D-alanine ligase confer high-level resistance to D-cycloserine.

PMID 33244063 · 2020

The study demonstrates that the ddl6 and ddl7 genes, when expressed in E. coli, confer resistance to D-cycloserine. The resistance is attributed to a single nucleotide polymorphism at c.777, which results in a Trp259Cys substitution in the Ddl6 protein.

Mutation Information

ddl6: p.W259C / c.G777T

Protein Change: W259C
Nucleotide Change: G777T
Organisms: Escherichia coli, Treponema denticola
Confers Resistance To: cycloserine
Effect on Function: The mutation at c.777 (G>T) leads to a Trp259Cys substitution, which increases the MIC of D-cycloserine by fourfold.
Validation Method: Site-directed mutagenesis and MIC testing
Source Database: Reslit

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